Abstract
Genetic diversity of 70 populations of niger (Guizotia abyssinica) representing all its growing regions in Ethiopia was investigated using random amplified polymorphic DNA (RAPD) to reveal the extent of its populations genetic diversity. Ninety-seven percent of the loci studied was revealed to be polymorphic for the whole data set. The within population diversity estimated by Shannon diversity index and Nei gene diversity estimates was revealed to be 0.395 and 0.158, respectively. The extent of genetic variation of populations from major niger producing regions was significantly lower than that of populations from other regions; however, it is distributed regardless of altitude of growth. Genetic differentiation between populations was estimated with Shannon index as G′ ST (0.432), Nei’s G ST (0.242) and AMOVA based F ST (0.350) and appears to be equivalent to the average values calculated from various RAPD based studies on outcrossing species. Higher proportion of the variation detected by AMOVA resided within populations (64.58%) relative to the amount of variation among populations (35.42%). UPGMA cluster analysis showed that most of the populations were clustered according to their region of origin. However, some populations were genetically distant from the majority and seem to have unique genetic properties. It is concluded that the crop has a wide genetic basis that may be used for the improvement of the species through conventional breeding and/or marker assisted selection. Collection of germplasm from areas not yet covered and/or underrepresented is the opportunity to broaden the genetic basis of genebank collection.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Aga E, Bryngelsson T, Bekele E, Salomon B (2003) Genetic diversity of forest arabica coffee (Coffea arabica L.) in Ethiopia as revealed by random amplified polymorphic DNA (RAPD) analysis. Hereditas 138:36–46
Alemaw G, Teklewold A (1995) An agronomic and seed-quality evaluation of noog (Guizotia abyssinica Cass.) germplasm from Ethiopia. Plant Breed 114:375–376
Baagøe J (1974) The genus Guizotia (Compositae). A taxonomic revision. Bot Tidsskr 69:1–39
Birmeta G, Nybom H, Bekele E (2004) Distinction between wild and cultivated enset (Ensete ventricosum) gene pools in Ethiopia using RAPD markers. Hereditas 140:139–148
Buckler ES IV, Thornsberry JM (2002) Plant molecular diversity and applications to genomics. Curr Opin Plant Biol 5:107–111
Bussell JD (1999) The distribution of random amplified polymorphic DNA (RAPD) diversity amongst populations of Isotoma petraea (Lobeliaceae). Mol Ecol 8:775–789
Dagne K (1994) Meiosis in interspecific hybrids and genomic interrelationships in Guizotia Cass. (Compositae). Hereditas 121:119–129
Dagne K (1995) Karyotypes, C-banding and nucleolar numbers in Guizotia (Compositae). Plant Syst Evol 195:121–135
Dagne K (2001) Cytogenetics of new Guizotia Cass. (Compositae), interspecific hybrids pertaining to genomic and phylogenetic affinities. Plant Syst Evol 230:1–11
Dagne K, Jonsson A (1997) Oil content and fatty acid composition of seeds of Guizotia Cass. (Compositae). J Sci Food Agric 73:274–278
Dutta PC, Helmersson S, Kebedu E, Alemaw G (1994) Variation in lipid composition of niger seed (Guizotia abyssinica Cass.) samples collected from different regions in Ethiopia. JAOCS 71:839–843
Geleta M, Asfaw Z, Bekele E, Teshome A (2002) Edible oil crops and their integration with the major cereals in North Shewa and South Welo, central Highlands of Ethiopia: an ethnobotanical perspective. Hereditas 137:29–40
Genet T, Belete K (2000) Phenotypic diversity in the Ethiopian noug germplasm. Afr Crop Sci J 8:137–143
Getinet A, Sharma SM (1996) Niger (Guizotia abyssinica (L. f.) Cass. Promoting the conservation and use of underutilized and neglected crops. 5. Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute, Rome
Hamrick JL, Godt MJW (1996) Effects of life history traits on genetic diversity in plant species. Philos T Roy Soc B 351:1291–1298
Harlan JR (1969) Ethiopia: a centre of diversity. Econ Bot 23:309–314
Hiremath SC, Murthy HN (1988) Domestication of niger (Guizotia abyssinica). Euphytica 37:225–228
Hiremath SC, Murthy HN (1992) Cytological studies in Guizotia (Asteraceae). Caryologia 45:69–82
Huff DR, Peakall R, Smouse PE (1993) RAPD variation within and among natural populations of outcrossing buffalograss [Buchloe dactyloides (Nutt.) Engelm.]. Theor Appl Genet 7:927–934
Kandel H, Porter P (eds) (2002) Niger (Guizotia abyssinica) (L. f.) Cass. Production in northwest Minnesota. University of Minnesota Extension Service
Lynch M, Milligan BG (1994) Analysis of population genetic structure with RAPD markers. Mol Ecol 3:91–99
Mohammadi SA, Prasanna BM (2003) Analysis of genetic diversity in crop plants-salient statistical tools and considerations. Crop Sci 43:1235–1248
Murthy HN, Hiremath SC, Salimath SS (1993) Origin, evolution and genome differentiation in Guizotia abyssinica and its wild species. Theor Appl Genet 87:587–592
Nayakar NY (1976) Genetic variability and heritability for six quantitative characters in niger (Guizotia abyssinica Cass). Mysore J Agric Sci 10:553–558
Nei M (1972) Genetic distance between populations. Am Nat 106:283–292
Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70:3321–3323
Nei M (1978) Estimation of average hetrozygosity and genetic distance from a small number of individuals. Genetics 89:583–590
Nemomissa S, Bekele E, Dagne K (1999) Self-incompatibility system in Ethiopian populations of Guizotia abyssinica (L. f.) Cass. (niger). Sinet: Ethiop J Sci 22:67–88
Nybom H (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Mol Ecol 13:1143–1155
Nybom H, Bartish IV (2000) Effects of life history traits and sampling strategies on genetic diversity estimates obtained from RAPD markers in plants. Perspect Plant Ecol 3:93–114
Nybom H, Werlemark G, Olsson ÅME (2001) Between and within population diversity in dogrose species. Acta Hortic 546:139–144
Page RDM (1996) TREEVIEW. An application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
Pavlicek A, Hrda S, Flegr J (1999) FreeTree – Freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap/jackknife analysis of the tree robustness. Application in the RAPD analysis of the genus Frenkelia. Folia Biol, Prague 45:97–99
Pradhan K, Mishra RC, Paikary RK (1995) Genetic variability and character association in niger. Indian J Genet Pl Br 55:457–459
Ramadan MF, Mörsel J-T (2002) Proximate neutral lipid composition of niger (Guizotia abyssinica Cass.) seed. Czech J Food Sci 20:98–104
Riley KW, Belayneh H (1989) Niger. In: Röbbelen G, Downey RK, Shri A (eds) Oil crops of the world. McGraw Hill Publishing Company, New York, pp 394–403
Rohlf FJ (2000) NTSYSpc, Numerical taxonomy and multivariate analysis system. Version 2.1. Exeter Software, Setauket, New York, USA
Rohlf FJ, Fisher DR (1968) Tests for hierarchical structure in random data sets. Syst Zool 17:407–412
Schneider S, Roessli D, Excoffier L (2000) Arlequin: a software for population genetics data analysis. Ver 2.000. Genetics and Biometry Lab, Dept. of Anthropology, University of Geneva
Sivolap YM, Solodenko AE, Burlov VV (1998) RAPD analysis of molecular-genetic polymorphism in sunflower Helianthus annuus. Genetika 34:266–271
Skroch P, Neinhuis J (1995) Impact of scoring error and reproducibility of RAPD data on RAPD based estimates of genetic distance. Theor Appl Genet 91:1086–1091
Snowdon RJ, Friedt W (2004) Molecular markers in Brassica oilseed breeding: current status and future possibilities. Plant Breed 123:1–8
Yeh FC, Boyle TJB (1997) Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belg J Bot 129:157
Zeven AC, DeWet JMJ (1982) Dictionary of cultivated plants and their regions of diversity. International Book Distributors, Dehra Dun, India
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Geleta, M., Bryngelsson, T., Bekele, E. et al. Genetic diversity of Guizotia abyssinica (L. f.) Cass. (Asteraceae) from Ethiopia as revealed by random amplified polymorphic DNA (RAPD). Genet Resour Crop Evol 54, 601–614 (2007). https://doi.org/10.1007/s10722-006-0018-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10722-006-0018-0