Abstract
Six sequential field experiments were conducted from 1999 to 2002 to evaluate virus reinfection in garlic cv. Amarante and its effect on bulb production. The treatments in the year 1999 were: T1 – virus-free garlic-seed obtained by thermotherapy and meristem-tip culture and indexed for virus by immuno-sorbent electron microscopy (ISEM) under its first field cycle; T2, T3 and T4 – garlic-seed in the second, third and fourth field cycles, respectively; and C – standard garlic-seed from the grower (with no control of virus infection) as a control. In the years 2000 to 2002, a new plot of virus-free seed was added to the experiment and cloves from the previous treatments were again grown under field conditions. In the fourth year of experiments, the treatments comprised T1 (virus-free garlic seeds under the first field cycle) to T7 (garlic-seed in the sixth field cycle) and C. Two experiments were conducted in the years 1999 and 2000, at two locations and in the years 2001 and 2002 only one experiment per year at one location. The combined analysis of variance for all experiments indicated a significant difference between the treatments for plant height and yield. The bulbs were classified into commercial classes according the Brazilian regulation and the commercial classes 4–7 were 72 %, 60%, 59%, 53% and 35% of the total number of bulbs harvested, from T1 to T5, respectively. Virus reinfection at the end of the second field cycle, determined by serology using antisera against the most common viruses of garlic in Brazil reached 83%. Treatments T1–T7 yielded 118%, 79%, 57%, 51%, 39%, 33% and 31% higher than the control.
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Acknowledgements
This work was partially funded by CBAB/CNPq, Brazil. We also tank Dr. David Bertioli from the Universidade Católica de Brasília for the critical review of both English and content of the manuscript.
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Filho, P.d.A.M., Resende, R.O., Cordeiro, C.M.T. et al. Viral reinfection affecting bulb production in garlic after seven years of cultivation under field conditions. Eur J Plant Pathol 116, 95–101 (2006). https://doi.org/10.1007/s10658-006-9042-3
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DOI: https://doi.org/10.1007/s10658-006-9042-3