Abstract
We have examined the effect of Pen, an aqueous extract of the dry mycelium of Penicillium chrysogenum, on plant–pathogen interactions. Pen controlled a broad range of pathogens on several crop plants under greenhouse and field conditions. Pen protected grapevine from downy and powdery mildew (caused by Plasmopara viticola and Uncinula necator), tomato from early blight (caused by Phytophthora infestans), onion from downy mildew (Peronospora destructor) and apple trees from apple scab (caused by Venturia inaequalis) to a similar extent as fungicides such as copper and sulphur or well-known inducers such as benzothiadiazole or β-aminobutyric acid. Pen had no major direct fungicidal effect and is thus supposed to protect plants by activating their defense mechanisms. The raw material for extraction of Pen was available in constant quality, a prerequisite for commercial application. Under certain conditions, Pen caused phytotoxic side effects. The symptoms mostly consisted of small necrotic spots or, more rarely, of larger necrotic areas. The development of the symptoms was dependent on several parameters, including concentration of Pen, the number of applications, the persistence on the plant tissue, the plant species and variety and environmental conditions. In grapevine, a partially purified fraction of Pen was much less toxic than the crude Pen extract, but protected the plants to a similar extent against P. viticola. Our data show that Pen has interesting and unique properties as a plant protection agent, but more research is needed to further reduce its phytotoxic side effects.
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Abbreviations
- Pen:
-
an aqueous extract of the dry mycelium of Penicillium chrysogenum
- Pen2000 :
-
a size fraction of Pen (>2000 Da) gained by dialysis
References
WS Abbott (1925) ArticleTitleA method for computing the effectiveness of an insecticide Journal of Economic Entomology 18 265–267 Occurrence Handle1:CAS:528:DyaB2MXhvFGlsQ%3D%3D
GN Agrios (1997) Plant Pathology Academic Press San Diego
Codex Alimentarius Commission (1999) Guidelines for the production, processing, labelling and marketing of organically produced foods. CAC/GL 32-1999, Rev. 1-2001.
Y Cohen U Gisi T Niderman (1993) ArticleTitleLocal and systemic protection against Phytophthora infestans induced in potato and tomato plants by jasmonic acid and jasmonic methyl ester Phytopathology 83 1054–1062 Occurrence Handle1:CAS:528:DyaK2cXitFaktb4%3D
Y Cohen T Niderman E Mösinger R Fluhr (1994) ArticleTitleβ-Aminobutyric acid induces the accumulation of pathogenesis-related proteins in tomato (Lycopersicon esculentum L.) plants and resistance to late blight infection caused by Phytophthora infestans Plant Physiology 104 59–66 Occurrence Handle1:CAS:528:DyaK2cXht12kt7Y%3D Occurrence Handle12232061
F Daayf A Schmitt RR Bélanger (1997) ArticleTitleEvidence of phytoalexins in cucumber leaves infected with powdery mildew following treatment with leaf extracts of Reynoutria sachalinensis Plant Physiology 113 719–727 Occurrence Handle12223638
H Dong TP Delaney DW Bauer SV Beer (1999) ArticleTitleHarpin induces disease resistance in Arabidopsis through the systemic acquired resistance pathway mediated by salicylic acid and the NIM1 gene The Plant Journal 20 207–215 Occurrence Handle1:CAS:528:DyaK1MXotFait7g%3D Occurrence Handle10571880 Occurrence Handle10.1046/j.1365-313x.1999.00595.x
RW Emmet TJ Wicks PA Magarey (1992) Downy mildew of grapes J Kumar HS Chanbe US Singh AN Mukhopadhyay (Eds) Diseases of fruit crops NumberInSeriesVol. 3 Prentice Hall NJ 90–128
EU (1991) Council Regulation (EEC) No. 2092/91 of 24 June 1991 on organic production of agricultural products and indications referring thereto on agricultural products and foodstuffs as amended.
EU (1997) Council Regulation (EEC) No. 1488/97 of 29 July 1997 on organic production of agricultural products and indications referring thereto on agricultural products and foodstuffs as amended.
European and mediterranean plant protection organization (EPPO) (1999) Guideline for the efficacy evaluation of plant protection products.
L Friedrich K Lawton W Ruess P Masner N Specker M Gut Rella B Meier S Dincher T Staub S Uknes J-P Métraux H Kessmann J Ryals (1996) ArticleTitleA benzothiadiazole derivative induces systemic acquired resistance in tobacco The Plant Journal 10 61–70 Occurrence Handle1:CAS:528:DyaK28XltVamsbc%3D Occurrence Handle10.1046/j.1365-313X.1996.10010061.x
J Gorlach S Volrath G Knauf-Beiter G Hengy U Beckhove KH Kogel M Oostendorp T Staub E Ward H Kessmann J Ryals (1996) ArticleTitleBenzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat The Plant Cell 8 629–643 Occurrence Handle1:STN:280:BymB3cnotFA%3D Occurrence Handle8624439
R Hammerschmidt (1995) Induced Resistance to Disease in Plants. Kluwer Academic Publishers Dordrecht
IFOAM (2000) Basic Standards for Organic Agriculture. D-Tholey-Theley.
J Kuc (1983) Induced systemic resistance in plants to diseases caused by fungi and bacteria JA Bailey BJ Deverall (Eds) The Dynamics of Host Defense Academic Press New York 191–221
J Kuc (2001) ArticleTitleConcepts and direction of induced systemic resistance in plants and its application European Journal of Plant Pathology 107 7–12
OMRI (2001) Generic Materials List with the National Organic Program Final Rule Listings. Eugene.
B Räz H Schüepp W Siegfried (1987) ArticleTitleHundert Jahre Plasmopara-Bekämpfung und Kupfereintrag in die Rebberge Schweizerische Zeitschrift für Obst- und Weinbau 123 272–277
AF Ross (1961) ArticleTitleSystemic acquired resistance induced by localized virus infections in plants Virology 14 340–358 Occurrence Handle1:STN:280:CC%2BD28zislA%3D Occurrence Handle13743578
S Schneider WR Ullrich (1994) ArticleTitleDifferential induction of resistance and enhanced enzyme activities in cucumber and tobacco caused by treatment with various abiotic and biotic inducers Physiological and Molecular Plant Pathology 45 291–304 Occurrence Handle1:CAS:528:DyaK2MXis1Ortrc%3D Occurrence Handle10.1016/S0885-5765(05)80060-8
Y Sekizawa S Mase (1980) ArticleTitleRecent progress in studies on non-fungicidal controlling agent Probenazole, with reference to the induced resistance mechanisms of rice plants Review of Plant Protection Research 13 114–121 Occurrence Handle1:CAS:528:DyaL3MXkslGhs7w%3D
RS Singh (2000) Diseases of Fruit Crops Science Publishers Enfield (NH)
B Speiser A Berner A Häseli L Tamm (2000) ArticleTitleControl of downy mildew of grapevine with potassium phosphonate: Effectivity and phosphonate residues in wine Biological Agriculture and Horticulture 17 305–312
Speiser B, Tamm L, Maurer V, Berner A and Walkenhorst M (2004) Hilfsstoffliste: Zugelassene und empfohlene Hilfsstoffe für den biologischen Landbau. Research Institute of Organic Agriculture (FiBL), Frick.
L Tamm (2001) ArticleTitleOrganic agriculture: development and state of the art Journal of Environmental Monitoring 3 92–96
Thuerig B, Felix G, Binder A, Boller T and Tamm L (submitted) An extract of Penicillium chrysogenum elicits early defense-related responses and induces resistance in Arabidopsis thaliana independently of known signalling pathways.
LC Loon Particlevan PAHM Bakker CMJ Pieterse (1998) ArticleTitleSystemic resistance induced by rhizosphere bacteria Annual Review of Phytopathology 36 453–483 Occurrence Handle15012509
E Ward SJ Uknes SC Williams SS Dincher DL Wiederhold DC Alexander P Ahl Goy JP Métraux J Ryals (1991) ArticleTitleCoordinate gene activity in response to agents that induce systemic acquired resistance The Plant Cell 3 1085–1094 Occurrence Handle1:CAS:528:DyaK38XjvVSkug%3D%3D Occurrence Handle12324583
RF White (1979) ArticleTitleAcetylsalicylic acid (aspirin) induces resistance to tobacco mosaic virus in tobacco Virology 99 410–412 Occurrence Handle1:CAS:528:DyaL3cXntVantA%3D%3D Occurrence Handle10.1016/0042-6822(79)90019-9
JH Zar (1996) Biostatistical Analysis Prentice-Hall Inc Upper Saddle River, NJ
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Thuerig, B., Binder, A., Boller, T. et al. An Aqueous Extract of the Dry Mycelium of Penicillium chrysogenum Induces Resistance in Several Crops under Controlled and Field Conditions. Eur J Plant Pathol 114, 185–197 (2006). https://doi.org/10.1007/s10658-005-4512-6
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DOI: https://doi.org/10.1007/s10658-005-4512-6