Abstract
In order to improve corn ethanol profitability and energy efficiency, a natural gum consisting of primarily arabinoxylan and crude protein (CP) was extracted from distillers’ grains (DG), a major byproduct from the dry grind corn ethanol production. DG was fractionated into an alkali-soluble gum fraction and an alkali-insoluble residue fraction by extracting with 1–5 % NaOH at 25–75 °C for 1–5 h. The extraction conditions, which significantly affect the yields and compositions of DG gum and residue, were statistically modeled to optimize yields and compositions. DG gum had 8–22 % CP, which could all be reduced to about 8 % by purification with bentonite clay. The isolated gums (purified and unpurified) were made into emulsifying agents, whereas residues were characterized as animal feed. The results demonstrated that the purification process affects the emulsifying properties of the DG gum-derived emulsifying agents. In parallel, the DG residue was found to have increased fiber digestibility and metabolizable energy compared to the original DG. An economic analysis determined that concurrent productions and utilizations of DG gum and DG residue could improve the cost and energy balance of the current corn ethanol process.
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Introduction
Bioethanol is a potential substitute for fossil fuels and its use in the transportation sector has become a strategy for the United States to reduce its energy dependence on imported petroleum and greenhouse gases (GHG) emission. Leading the world in bioethanol production, the annual output of the United States was approximately 56 billion liters in 2012 using corn grain as the chief feedstock (RFA 2013). However, the rising of corn price has reduced the profits and increased ethanol cost of manufacture (Brown and Brown 2012). Additionally, the energy balance to produce corn ethanol (the ratio of biofuel energy output to the fossil energy input) is about 1.3 (Shapouri et al. 2003), which is much lower than sugarcane ethanol (~8.0), biodiesel (~3) and cellulosic ethanol (~2–36) (Brown and Brown 2012). However, this ratio can be improved by considering byproducts’ energy credits (Shapouri et al. 2003). Consequently, if extra high value products with low energy input, are produced from corn ethanol biorefinery system, the corn ethanol profitability and energy efficiency will be greatly enhanced.
Distillers’ grains (DG) are major byproducts from dry grind corn ethanol production. The DG product has roughly one-third of its weight being crude protein (CP), and has abundant levels of fat, cellulose and hemicelluloses (Kim et al. 2010). DG is primarily used as low-value animal feed for protein source and its high fiber content limits its utilization by non-ruminants (Lamsal et al. 2012). Thus, converting this polysaccharide portion of DG into high-valued bio-products while still retaining the CP for animal feed utilization would be an effective way of valorizing corn ethanol byproducts.
Extensive studies have been carried out valorizing DG by converting the carbohydrates into ethanol or furfural (Dien et al. 2008; Kim et al. 2008; Tucker et al. 2004; Xiang and Runge 2014). However, as these processes typically require costly and severe chemical, biochemical, and/or mechanical treatments, their economy and fossil energy usage is less favorable. On the other hand, extracting the polymeric form of polysaccharides from DG for material use could be an alternative way to valorize DG, but few studies have considered this (Xiang et al. 2014), possibly because as such large amounts of DG have only recently become widely available due to ethanol production boost (Shapouri et al. 2003).
The most widely used method to fractionate hemicelluloses from plant biomass is using alkali or alkaline peroxide (Sun et al. 2002). The major hemicelluloses extracted from corn biomass are highly branched arabinoxylans (Ebringerová and Heinze 2000). Extensive studies have described the potential use of corn hemicelluloses for various types of polymeric materials such as films and emulsifying agents (Woo 2001; Whistler 1993). Unlike those relatively pure arabinoxylans directly extracted from corn bran or corn kernels, the hemicellulosic gum extracted from DG, however, could be considerably heterogeneous. The DG contains relatively high amounts of CP, which could be co-extracted with hemicelluloses and may affect the properties and processabilities of the DG gum. Methods to remove protein contamination in hemicellulosic materials were extensively studied (Izydorczyk and Biliaderis 1995) and one of the effective ways is using clay (Izydorczyk et al. 1991). However, CP may not necessarily be a contamination for the extracted DG gum materials, as it has been reported that protein can increase the corn fiber gum’s emulsifying properties (Yadav et al. 2007).
We recently reported a study fractionating DG into an alkaline-soluble hemicellulose-rich DG gum and an alkaline-insoluble protein-rich DG residue, and examined concurrent utilizations of DG gum as paper coatings and DG residues as animal feed (Xiang et al. 2014). In this study, we have expanded upon the previous work and conducted additional studies to evaluate the effects of different alkali extraction conditions on the yield and composition of the DG gum and residues. Additionally, the animal feed characterization of DG residue has been more thoroughly evaluated. We also proposed a novel utilization of the isolated gum as emulsifying agents and investigated the effects of purification on its emulsifying properties. Based on our approach to valorize DG, a new process was considered and its economic feasibility examined.
Experimental section
Fractionation of DG into DG gum and DG residue
Distiller’s grains (DG) samples were obtained dried from Didion Milling Inc. (Cambria, WI, USA). In order to create statistical models including temperature, alkaline concentration and reaction time as variables to predict the gum and residue yields and qualities, two sets of screening experiments with factorial matrices were designed. The first set of the experiments included a full 22 factorial matrix with constant reaction time of 3 h (temperature: 25 and 75 °C, alkaline: 1 and 5 %) as well as one central point (50 °C, 3 % and 3 h). The second set was designed to include the reaction time into the model and it had a 22 full factorial matrix with constant reaction temperature as 50 °C (alkaline: 1 and 5 %, reaction time: 1 and 5 h) including one central point with the same conditions as in the first matrix. Duplicate runs were performed for each combination and triplicate runs for the central point. Based on the experimental conditions described by the matrices, for each run, the DG and NaOH solution (1:20 solid to liquid weight ratio; 1:10 and 1:6 ratios were also tested for condition 50 °C, 3 % and 3 h) were mixed, and maintained at constant temperatures by stirring on a magnetic stirring hot plate for a desired reaction time. The mixture was then separated into alkaline soluble and insoluble fractions through centrifugation. The collected alkaline insoluble fraction or residue (DG residue) was washed with water and freeze-dried for further animal feed characterization. The alkali-soluble part was adjusted to pH 5.5 by 6 M HCl, concentrated to approximately one-third of its original volume on a rotary evaporator at reduced pressure, and slowly poured into a three-time volume of 95 % ethanol with constant stirring. The precipitated solid was separated and washed with 95 % ethanol through filter paper to give an alkali-soluble gum fraction (DG gum). Several conditions were investigated for DG gum purification process to remove CP. After the alkali-soluble fractions were adjusted to pH 5.5, bentonite clay (~20 g/L) was added. The solution was stirred at 500 rpm for 1 h, and then centrifuged and filtered to remove the clay. The clay free solution then was used for the concentration and ethanol precipitation steps.
Structural analysis of DG gum
Samples being determined for neutral sugar contents were hydrolyzed according to the procedure described previously (Min et al. 2014) and the hydrolysate was then run through a ICS-3000 Ion Chromatography (IC) System (Dionex, Sunnyvale, CA, USA) according to the method described by Xiang et al. (2014).
Crude protein content was determined by a combustion method according to AOAC official method 990.03 using a FlashEA 1112 Series CN analyzer (Thermo Electron, Waltham, MA, USA).
Molecular weights of DG gum were determined by gel permeation chromatography (GPC). DG gum samples were acetylated in a DMSO/N-methylimidazole/acetic anhydride system, and the acetylated DG gum samples were subjected to GPC analysis on a Shimadzu LC-20A LC system equipped with a refractive index (RI) detector (RID-M20A, Shimadzu, Columbia, MD, USA) using the conditions previously described (Xiang et al. 2014; Tobimatsu et al. 2013). The data acquisition and computation used Shimadzu LCsolution version 1.25 software.
FT-IR spectra of DG gum were collected on a Spectrum 100 FT-IR spectrometer (PerkinElmer, Waltham, MA, USA) equipped with a universal ATR sampling accessory. The spectrum was acquired in the range of 650–4,000 cm−1 with a total of eight scans.
DG gum emulsifying properties
DG gum samples or a gum arabic sample (MP Biomedicals, Santa Ana, CA, USA) were suspended (5.0 wt%) in an aqueous solution containing 10.0 wt% sucrose, 0.1 wt% sodium benzoate and 0.3 wt% citric acid, and the suspension was then put into a sonicator for 2 h to dissolve and ensure a homogeneous solution. The gum solution was then diluted 50 times into a 0.1 wt% gum solution and 0.2 wt% orange oil (Now Foods, Bloomingdale, IL, USA) was added. The gum and oil mixture were then mixed with high shear in a Waring 700 s Blender for 2 min. The final composition of the gum/oil emulsion was 0.1 wt% gum, 0.2 wt% orange oil, 10 wt% sucrose, 0.1 wt% sodium benzoate and 0.3 wt% citric acid. The surface-area-average diameter (droplet size, d 32) and zeta potential of the emulsion was measured right after the emulsions were prepared by a particle size analyzer (90Plus, Brookhaven, Long Island, NY, USA) according to the procedure described by Sun and Gunasekaran (2009). The turbidity of the emulsion was determined by a UV–Vis spectrophotometer (UVmini-1240, Shimadzu, Columbia, MD, USA) following the procedure described by Yadav et al. (2007). The turbidity of the diluted gum solution before adding oil was also measured in order to calculate a relative turbidity for the gum/oil emulsion to exclude the effects of original cloudiness of the DG gum solutions.
Animal feed characterization of DG residue
The ash content was determined according to the NREL LAP (NREL/TP-510-42622) using an Isotemp Muffle Furnace (Fisher Scientific). Crude fat content was determined by using hexane to extract the sample in a Soxhlet apparatus for 24 h according to AOAC official method 2003.06. CP content was determined by AOAC official method 990.03. Soluble CP was evaluated based on the procedure described by Krishnamoorthy et al. (1983).
Acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents were determined according to AOAC official method 973.18 and AOAC 2002.04. Acid detergent insoluble crude protein (ADICP) was determined by measuring the CP content in the ADF portion. In vitro true dry matter digestibility (IVTDMD) analysis was performed using raw DG as a control, and with DG residue to determine its feasibility to be considered as an animal feed. IVTDMD measurement was done following the procedure described by Goering and Van Soest (1970) except that the incubation time was 30 h. NDF digestibility (NDFD) was calculated based on NDF and IVTDMD as (Goeser and Combs 2009).
Model and economic analysis
Statistical models and analysis of variance were generated by JMP Pro 10 (SAS). Process mass and energy flows were estimated by Aspen Plus V7.3 (AspenTech). Optimum economic model was created and calculated by Matlab R2010a (MathWorks).
Results and discussion
Fractionation of DG into DG gum and DG residue
The chemical compositions and animal feed characterizations (Tables 1, 2) of the raw DG material used in this study were consistent with previous studies (Kim et al. 2010; Robinson et al. 2008). Similar to our previous studies (Xiang et al. 2014), the fractionated DG gum, in general, had significantly enriched levels of arabinan (15–19 %) and xylan (19–27 %) with highly reduced CP content (8–22 %), while the fractionated DG residue were overall consisted of abundant levels of cellulosic fibers (ADF) and CP depending on the conditions (Tables 1, 2 and Online Resource 1).
Yields and chemical compositions of the fractionated DG gum and DG residue were much dependent on the alkaline gum extraction conditions (Tables 1, 2 and Online Resource 1). To better understand the effects of the reaction conditions on gum extraction and to predict the theoretical optimum condition, multiple regression models at 95 % confidence interval were generated to fit the yields and compositional data of DG gum and DG residue within the range of the experimental matrices (temperature: 25–75 °C, NaOH conc.: 1–5 %, and reaction time: 1–5 h). The complete analysis of variance models may be seen in Online Resource 2, with regression models shown as Eq. (1) through (4) in Online Resource 3.
Based on the models, 3-D graphs at constant reaction time (3 h) and constant reaction temperature (50 °C) were generated (Figs. 1, 2). Overall, the extraction severity had a positive relationship with the yield and of DG gum, although the effect of extraction time on both DG gum yield and polysaccharide/CP ratio appeared to be less significant compared to those of extraction temperature and NaOH concentration. Our model predicted that DG gum could be obtained in the maximum yield of ~30 % by an extraction at ~60 °C, with 3 % NaOH for 5 h. The polysaccharide/CP ratio of DG gum had its maximum towards the high end of reaction severity; gums extracted at 75 °C with 5 % NaOH for 3 h had the highest carbohydrate content (~62 %) with the lowest CP level (~8 %) in this study.
Compared to DG gum, the yields and CP contents of DG residue varied more under the different extraction conditions. As the yield of DG gum increased with increased extraction severity, the yield of DG residue accordingly decreased. However, whereas CP levels in DG gum decreased with increased extraction severity, CP contents of DG residue also appear to decrease considerably, suggesting that severe alkaline conditions degraded the protein into soluble amino acids and/or small peptides (Saulnier et al. 1995).
Purification of DG gum
Gums from four selected conditions with highest (50–1.0–5, 21.8 %), lowest (75–5.0–3, 7.8 %) and medium (50–5.0–5, 16.9 % and 75–1.0–3, 14.0 %) CP contents were purified using bentonite clay (Izydorczyk et al. 1991). As can be seen from Table 1, CP contents of purified gums from the selected conditions were all decreased to about 8 %, whereas the total sugar contents were increased up to ~60 % (arabinan and xylan contents, ~20 and ~27 %), leading to similar chemical compositions for all selected purified DG gums. In addition, FT-IR analysis of the DG gum films determined that the peaks from N–H bending in amides at 1,547 cm−1 (Jagadeesh et al. 2011) vanished with the clay purification process (Online Resource 4). These results indicate the effective removal of CP by bentonite clay. The 8 % CP from all the selected purified gums that were not removed by bentonite clay might be referred to structural proteins such as hydroxyproline-rich glycoprotein that are linked through alkali resistant glycosidic bonds to hemicelluloses (Saulnier et al. 1995; Yadav et al. 2007), or a portion possibly even the subunits of a newly confirmed hemicelluloses-protein complex in plants (Tan et al. 2013).
Molecular weight distributions of DG gum
The molecular weights of some selected DG gum samples were determined by GPC (Table 3 and Online Resource 5). The averaged molecular weight (M w and M n) and the polydispersity index (PDI, M w/M n) of acetylated DG gum samples were in the range of the values reported for other isolated hemicelluloses or gums (Yadav et al. 2009 and Xiang et al. 2014). The averaged molecular weights of DG gum increased as the extraction temperature and alkaline concentration increased, indicating that high molecular mass portions of DG hemicelluloses were more effectively extracted under severe alkaline conditions (Saulnier et al. 1995). After purification with bentonite clay, the M w and M n of DG gum did not vary, but PDI decreased considerably. Thus, the clay treatment was effective to produce gums composed of arabinoxylans with narrower molecular mass distributions. Given that the purified gum of 75–5.0–3 had little CP removed but showed a decreased PDI, however, the clay might somewhat remove some short chain polysaccharides or fatty acids together with CP (Asselman and Garnier 2000).
DG gum emulsifying properties
The surface-area-average particle size and zeta potential profiles for emulsions without emulsifying agents (control) and emulsions aided with gum arabic and with DG gum from selected conditions (purified and unpurified) were measured and shown in Fig. 3. Gum arabic is a common emulsifying agent used in beverage making industry and thus was used to compare with DG gum. Similar to gum arabic, DG gum were able to effectively reduce the droplet size in the oil–water emulsion showing potential emulsifying properties (Fig. 3a). Among emulsions with unpurified DG gum, sample 75–5.0–3 with the lowest CP content had the smallest droplet size of about 570 nm, while sample 50–5.0–5 with the highest CP content had the largest droplet size of about 930 nm. However, comparing within the same alkali conditions, emulsions with unpurified DG gum had smaller droplet size than with purified DG gum demonstrating a more homogeneous emulsion. For zeta potential (Fig. 3b), all emulsions aided with DG gum had significant lower zeta potential (−6 to −12 mV) than gum arabic (−23.8 mV) showing possible higher tendency for the oil droplets to aggregate, creating less stable emulsions.
For the emulsion stabilities as described by the changes in turbidity over time (Yadav et al. 2007), when fresh prepared (Fig. 4a), most of the emulsions have similar or higher relative turbidities than the control sample except samples 75–1.0–3 and 50–1.0–5. After 1 and 7 days (Fig. 4b, c), the turbidity of the control sample quickly dropped from ~110 to ~20 cm−1 and to below 5 cm−1 suggesting significant oil droplet aggregation, whereas all the other samples maintained relatively higher turbidities indicating the effectiveness of DG gum and gum arabic to stabilize the oil–water emulsion. The comparable emulsion stabilities between DG gum and gum arabic emulsions contradicted the prediction from zeta-potential data (will further explain later in this section). Comparing within the same alkali conditions, after a short time of preparation, the purified gum emulsions had higher turbidities than unpurified gum emulsions. However, after 7 days, the turbidities of the unpurified gum emulsions were overall higher than the purified gum emulsions showing better emulsifying stability.
The DG gums without purification were overall showing potential emulsifying properties by comparing with gum arabic. The structures of gum arabic and DG gum are mainly polysaccharides-protein complex that the former one contains arabinogalactan-protein complex, while the latter one contains arabinoxylan–protein complex (Islam et al. 1997; Saulnier et al. 1995). As for oil–water emulsions aided with gum arabic, the protein with its hydrophobicity was proved to be anchored to the oil droplet, which helps the stable penetration of hydrophilic polysaccharides into the aqueous phase preventing the oil droplets aggregation by steric effects (Randall et al. 1988). The role of protein in aiding the emulsifying process for gum arabic might also be applied to DG gum, as suggested by the results that unpurified DG gum had better emulsion forming properties (smaller particle size) and emulsion stabilities than purified DG gum. Additionally, the higher zeta-potential of emulsions aided with gum arabic than DG gum might be explained by the different electrical charges of their polysaccharides groups, in which gum arabic had much higher uronic acid content (~20 wt%, Randall et al. 1988) than DG gum (~5 wt%, Xiang et al. 2014). However, polysaccharide steric effect instead of charge effect was found to hold the major responsibility for protein-anchored oil droplet repulsion (Randall et al. 1988), which could explain the fact that the low zeta-potential of DG gum emulsions did not affect their stabilities.
DG residue animal feed characterization
To effectively utilize all fractions of DG, the DG residue after gum extraction also needs to be used, and thus was characterized for its potential as animal feed. Animal feed provides the animal with energy and thus investigating its metabolizable energy (ME) level is a basis to evaluate animal feed value. The ME of animal feed is mainly calculated from the digestion of fat, CP, non-fiber carbohydrate and digestible portion of fibrous carbohydrate (Robinson et al. 2004; Weiss et al. 1992). Recent advances in animal feed science have developed several relationships to determine the energy level of feed by considering the contribution of each nutritional component (NRC 2001; Robinson et al. 2004). In this study, the equation developed by Robinson et al. (2004) was used to estimate the ME level of DG and DG residue, and the same approach was applied to DG before by Robinson et al. (2008). The calculation mainly involved several individual components including: (1) NDF, which represents the fibrous carbohydrates including cellulose, hemicelluloses, and lignin; (2) NDF digestibility (NDFD), which determines the digestible portion of NDF (Goering and Van Soest 1970; Van Soest et al. 1991); (3) ADF, representing the least digestible fibrous portion mainly consisting of cellulose and lignin; and (4) ADICP, which is the protein fraction remaining in ADF and indigestible to the animal.
The individual nutritional components and calculated ME for selected DG residues are determined and shown in Table 2. Compared to raw DG, the DG residue overall had decreased crude fat content, enriched fiber content, increased fiber digestibility, and slightly higher levels of metabolized energy (ME). NDF, ADF and NDFD of DG residue all increased compared to raw DG. With only minimal amount of lignin in DG, the increase of ADF indicated the increase of cellulose content in DG residue and was the main reason for the increase of NDF according to the data (Table 2). The increase of NDFD suggested the fiber portion of DG residue was more digestible by cows, which could be the result of increase in the accessibility of the recalcitrant cellulose from the alkaline treatment conditions (Hendriks and Zeeman 2009). Among different alkali extraction conditions, NDF and ADF had higher values at severer conditions, while the values of NDFD vary only slightly that even the mildest condition (25–1.0–3) could elevate the NDFD from 40 % of raw DG to 80 %. The final calculated ME of DG residue increased about 7–16 % compared to raw DG suggesting DG residue held more energy per unit weight than raw DG. Additionally, as already discussed in the earlier section, CP content of DG residue increased as the reaction severity decreases. Consequently, at less severe alkali conditions, DG residue displays improved yield, energy and nutritional value as well as significantly enhanced fiber digestibility suggesting its potential animal feed utilization.
Economic analysis
The DG gum’s commercial implementation as a potential film or emulsifying agents depends greatly on the economics of the extraction process. Unfortunately, the optimum yields or qualities of DG gum and DG residue do not share similar conditions. Consequently, an economic model incorporating DG gum and DG residue yields as well as the variable costs such as energy cost, raw material costs and others was developed in order to optimize the conditions and evaluate the economics of the process. The energy cost was calculated by Aspen Plus and the Aspen model process flow chart can be seen in Online Resource 6, of which a simplified version can be seen in Fig. 5. As shown in the chart, ethanol was recycled after gum precipitation, and the ethanol soluble protein was recovered into animal feed as proposed by Xiang et al. (2014), and some extra alkaline soluble portion (for gum precipitation) was collected by washing the DG residue. For the model, although the solid/liquid ratio from the experiment matrix (Table 1) was 1:20, experiments with reduced ratios (1:6 and 1:10) were conducted and shown similar yields of DG gum and DG residue compared to ratio 1:20 (Online Resource 7). Thus lower ratios could be used. Additionally, in an industrial process, materials will be recycled. Some other considerations for the model were also taken into account and the assumptions as the basis for the model were summarized:
-
1.
The model was based on a corn ethanol plant with WDG yield of 150,000 kg/day.
-
2.
Costs for machine, time, and labor were not taken into account.
-
3.
The model is only valid for the alkali extraction conditions in this study (temperature: 25–75 °C, alkali concentration: 1–5 % and reaction time: 1–5 h).
-
4.
Solid to liquid ratio for alkali extraction is assumed to be 1:6 and the better mixing of industrial reactor would give similar yield results compared to yields from the experiments.
-
5.
DG gum price was assumed to be 3 $/kg for the base case but was varied to determine its effect (Fig. 6). DG gum price was assumed the same for gums from different conditions; animal feed price was assumed the same for DG residue from different conditions.
As shown in Table 4 for the model calculation, the cost of raw materials, including mostly ethanol for precipitation, far exceeded other cost of productions. Additionally, unlike producing furfural from DG (Xiang and Runge 2014), animal feed production in this study did not have a significant impact on the net revenue. Thus, further improvement of the process should focus on increasing the yield of gum production and reducing the ethanol usage. The model predicted the highest revenue was 9,829 US$/day more than marketing DDG solely as animal feed amounting to an additional annual revenue of more than 3.5 million US$ for a corn ethanol plant with capacity of 150,000 kg WDG/day. The value of DDG increased from 0.25 US$/kg for animal feed to 0.44 US$/kg for combined gum and feed production. However, this calculation is based on the gum price of 3 US$/kg (a typical price for gum arabic). Since the gum price is not certain, a graph was generated to display the net revenue for different gum prices (Fig. 6), as shown from which, the gum price had to be at least 2.3 US$/kg in order to make profits.
Conclusions
The gum extracted by NaOH solution from DG contained about 50–60 % polysaccharides, mainly arabinoxylans, and 10–20 % CP. Alkaline extraction conditions (alkali concentration, temperature and reaction time) were shown to have significant effects on DG gum and DG residue yields and chemical compositions. High polysaccharide/CP ratio of DG gum was created at a high reaction severity, while high yield and CP content of DG residue appeared at a low severity. Bentonite clay was effective in removing CP from DG gum, but it was only able to lower the CP content to about 8 %.
Distillers’ grains gum had unique emulsifying properties potential for practical applications, which were affected by the purification process. Unpurified DG gum was superior to the purified gum with better emulsifying properties. Additionally, DG residue had higher fiber content, fiber digestibility and ME level than raw DG. However, the CP content, an important nutrition in animal feed, depended greatly on extraction conditions. From the economic analysis, the DG gum production process is highly economically feasible and would make the corn ethanol industry more profitable.
Abbreviations
- ADF:
-
Acid detergent fiber
- ADICP:
-
Acid detergent insoluble crude protein
- CP:
-
Crude protein
- DG:
-
Distillers’ grains
- ME:
-
Metabolizable energy
- NDF:
-
Neutral detergent fiber
- NDFD:
-
Neutral detergent fiber digestibility
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Acknowledgments
This work was supported by US Department of Agriculture, under contract USDA Critical Agricultural Material Grant (2013-38202-20400). GPC analyses were carried out at the Great Lakes Bioenergy Research Center with support from the funding from US Department of Energy, the Office of Science (BER DE-FC02-07ER64494). The authors would also like to give their appreciations to Dr. John Ralph and Dr. Ali Azarpira for GPC analyses, to Yi-cheng Wang and Dr. Sundaram Gunasekaran for FTIR and particle size analyses, and to Didion Milling Inc. for materials and valuable discussions.
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Xiang, Z., Anthony, R., Tobimatsu, Y. et al. Emulsifying properties of an arabinoxylan–protein gum from distillers’ grains and the co-production of animal feed. Cellulose 21, 3623–3635 (2014). https://doi.org/10.1007/s10570-014-0379-z
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DOI: https://doi.org/10.1007/s10570-014-0379-z