Abstract
The effect of UV-B irradiation at temperatures of 22 and 4 °C on flash induced oxygen yields, photochemical activity, and energy transfer in pea thylakoid membranes in the absence and presence of scavengers of reactive oxygen species (ROS) was studied. Three different scavengers were used: dimethyl sulfoxide (DMSO), histidine (His), and n-propyl gallate (nPG). As result of the UV-B treatment of isolated membranes, the flash oxygen yields were considerably affected — the amplitudes decreased and the oscillation pattern was lost. The analysis of the flash oxygen yields and initial oxygen burst showed alterations of a number of oxygen evolving centers in the S0 state as well as changes of decay kinetics of the oxygen burst under continuous irradiation. ROS scavengers exhibited more or less expressed protective effects, nPG being the most effective against UV-B induced damages of the flash oxygen yields. At both the temperatures, photosystem II (PS II) mediated electron transport was more sensitive to the UV-B treatment in comparison with photosystem I (PS I). The analysis of 77 K fluorescence spectra showed that the fluorescence ratio F735/F685 increased by the UV-B treatment probably due to a redistribution of excitation energy between both photosystems most likely caused by partial unstacking and due to a decrease of PS II fluorescence resulting from reaction center-type quenching. The nPG was the most powerful scavenger which protected the oxygen evolution capacity of PS II in the absence and presence of an exogenous electron acceptor to the highest extent.
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Abbreviations
- BQ:
-
1,4 benzoquinone
- Chl:
-
chlorophyll
- DCMU:
-
3-(3,4dichlorophenyl)-1,1-dimethyl urea
- DCPIP:
-
2,6-dichlorophenol-indophenol
- DMSO:
-
dimethyl sulfoxide
- His:
-
histidine
- MDA:
-
malondialdehyde
- MES:
-
2-[N-morpholino] ethanesulfonic acid
- nPG:
-
n-propyl gallate
- PS:
-
photosystem
- ROS:
-
reactive oxygen species
- Si :
-
redox states of water oxidizing complex
- Tricine:
-
N-[tris(hydroxymethyl) methyl] glycine
- Tris:
-
2-amino-2-hydroxymethyl-propane-1,3-diol
- UVBBE :
-
biological effectiveness of UV-B radiation
References
Andrizhiyevskaya, E.G., Chojnicka, A., Bautista, J.A., Diner, B.A., Van Grondelle, R., Dekker, J.P.: Origin of the F685 and F695 fluorescence in Photosystem II. — Photosynth. Res. 84: 173–180, 2005.
Barbato, R., Frizzo, A., Friso, G., Rigoni, F., Giacocometti, G.M.: Degradation of the D1 protein of photosystem II reaction centre by ultraviolet-B radiation requires the presence of functional manganese on the donor side. — Eur. J. Biochem. 227: 723–729, 1995.
Barta, C., Kalai, T., Hideg, K., Vass, I., Hideg, E.: Differences in the ROS-generation efficacy of various ultraviolet wavelengths in detached spinach leaves. — Funct. Plant Physiol. 31: 23–28, 2004.
Bruce, D., Samson, G., Carpenter, C.: The origins of nonphotochemical quenching of chlorophyll fluorescence in photosynthesis. Direct quenching by P680+ in photosystem II enriched membranes at low pH. — Biochemistry 36: 749–755, 1997.
Esterbauer, H., Cheeseman, K.: Determination of aldehyde lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. — Meth. Enzymol. 186: 407–431, 1990.
Fedina, I., Velitchkova, M., Georgieva, K., Demirevska, K., Simova, L.: UV-B response of green and etiolated barley seedlings. — Biol. Plant. 51: 699–706, 2007.
Fedina, I., Hidema, J., Velitchkova, M., Geprgieva, K., Nedeva, D.: UV-B induced stress in three rice cultivars. — Biol. Plant. 54: 571–574, 2010.
Friso, G., Spetea, C., Giacometti, G.M., Vass, I., Barbato, R.: Degradation of photosystem II reaction centre D1-protein induced by UV-B radiation in isolated thylakoids. Identification and characterization of C- and N- terminal breakdown products. — Biochim. biophys. Acta 1184: 78–84, 1994.
He, Y.Y., Häder, D.-P.: Involvement of reactive oxygen species in the UV-B damage to the cyanobacterium Anabaena sp. — J. Photochem. Photobiol. B-Biol. 66: 73–80, 2002.
Hideg, E., Sass, L., Barbato, R., Vass, I.: Inactivation of oxygen evolution by UV-B irradiation. A thermoluminescence study. — Photosynth. Res. 38: 455–462, 1993.
Hideg, E., Vass, I.: UV-B induced free radical production in plant leaves and isolated thylakoid membranes. — Plant Sci. 115: 251–260, 1996.
Hollosy, F.: Effects of ultraviolet radiation on plant cells. — Micron 33: 179–197, 2002.
Horton, P., Ruban, A.: Regulation of Photosystem II. — Photosynth. Res. 34: 375–385, 1992.
Jacob, B., Heber, U.: Photoproduction and detoxification of hydroxyl radicals in chloroplasts and leaves and relation to photoinactivation of photosystem I and II. — Plant Cell Physiol. 37: 629–635, 1996.
Jordan, B.R.: The effect of ultraviolet-B radiation on plants: a molecular perspective. — Adv. Bot. Res. 22: 97–162, 1996.
Kok, B., Forbush, B., McGloin, M.: Co-operation of charges in photosynthetic O2 evolution. I. A linear four step mechanism. — Photochem. Photobiol. 11: 457–475, 1970.
Krause, G.H., Weis, E.: Chlorophyll fluorescence and photosynthesis. The basis. — Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 313–349, 1991.
Lichtenthaler, H.K.: Chlorophylls and carotenoids: pigments of photosynthetic membranes. — Meth. Enzymol. 148: 350–382, 1987.
Mackerness, S.A.-H., Jordan, B.R., Thomas, B.: Reactive oxygen species in the regulation of photosynthetic genes by ultraviolet-B radiation (UV-B; 280–320 nm) in green and etiolated buds of pea (Pisum sativum L.). — J. Photochem. Photobiol. B-Biol. 48: 180–188, 1999.
Mishra, N.P., Francke, C., Van Gorkom, H.J., Ghanotakis, D.F.: Destructive role of singlet oxygen during aerobic illumination of the photosystem II core complex. — Biochim. biophys. Acta 1186: 81–90, 1994.
Miyao, M.: Involvement of active oxygen species in degradation of D1 protein under strong illumination in isolated subcomplexes of photosystem II. — Biochemistry 33: 9722–9730, 1994.
Mukai, K., Nagai, S., Ohara, K.: Kinetic study of the quenching reaction of singlet oxygen by tea catechins in ethanol solution. — Free Radical Biol. Med. 15: 752–761, 2005.
Nedunchezhian, N., Kulandaivelu, G.: Evidence for the ultraviolet- B (280–320 nm) radiation induced structural reorganization and damage of photosystem II polypeptides in isolated chloroplasts. — Physiol. Plant. 81: 558–562, 1991.
Rajagopal, S., Joly, D., Gauthier, A., Beauregard, M., Carpentier, R.: Protective effect of active oxygen scavengers on protein degradation and photochemical function in photosystem I submembrane fractions during light stress. — FEBS J. 272: 892–902, 2005.
Rajagopal, S., Murthy, S.D.S., Mohanty, P.: Effect of ultraviolet- B radiation on intact cells of the cyanobacterium Spirulina platensis: characterization of the alterations in the thylakoid membranes. — J. Photochem. Photobiol. B — Biol. 54: 61–66, 2000.
Renger, G., Völker, M., Eckert, H.J., Fromme, R., Hohm-Veit, S., Graber, P.: On the mechanism of photosystem II deterioration by UV-B irradiation. — Photochem. Photobiol. 9: 97–105, 1989.
Satoh, K., Oh-Hashi, M., Kashino, Y., Koike, H.: Mechanism of electron flow through the QB site in photosystem II. 1. Kinetics of the reduction of electron acceptors at the QB and plastoquinone sites in photosystem II particles from the cyanobacterium Synechococcus vulcanus. — Plant Cell Physiol. 36: 597–605, 1995.
Šnyrychová, I., Kós P.B., Hideg E.: Hydroxyl radicals are not the protagonists of UV-B-induced damage in isolated thylakoid membranes. — Funct. Plant Biol. 34: 1112–1121, 2007.
Sonoike, K.: Degradation of psaB gene product, the reaction center subunit of photosystem I, is caused during photoinhibition of photosystem I: possible involvement of active oxygen species. — Plant Sci. 115: 157–164, 1996.
Strid, A., Chow, W.S., Anderson, J.M.: UV-B damage and protection at molecular level in plants. — Photosynth. Res. 39: 475–489, 1994.
Szilard, A, Sass, L., Deak, Z., Vass, I.: The sensitivity of photosystem II to damage by UV-B radiation depends on the oxidation state of the water-splitting complex. — Biochim. biophys. Acta 1767: 876–882, 2007.
Vass, I., Kirilovsky, D., Etienne, A.-L.: UV-B radiation-induced donor- and acceptor-side modifications of photosystem II in the cyanobacterium Synechocystis sp. PCC 6803. — Biochemistry 38: 12786–12794, 1999.
Vass, I., Sass, L., Spetea, C., Bacou, A., Chanotakis D.F., Petrouleas.: UV-B-induced inhibition of photosystem II electron transport studied by EPR and chlorophyll fluorescence. Impairment of donor and acceptor side components. — Biochemistry 35: 8964–8973, 1996.
Vass, I., Szilard, A., Sicora, C.: Adverse effects of UV-B light on the structure and function of the photosynthetic apparatus. — In: Pessarakli M. (ed.): Handbook of Photosynthesis. 2nd Ed. Pp. 827–844. CRC Press, Taylor&Francis Group, Boca Raton — London — New York — Singapore 2005.
Velitchkova, M., Popova, A.: High light induced changes of 77 K fluorescence emission of pea thylakoid membranes with altered membrane fluidity. — Bioelectrochemistry 67: 81–90, 2005.
Xue, W., Li, X.Y., Zhu, J.T., Lin, L.S.: Effects of temperature and irradiance on photosystem activity during Alhagi sparsifolia leaf senescence. — Biol. Plant. 56: 785–788, 2012.
Zeinalov, Y.: An equipment for investigations of photosynthetic oxygen production reactions. — Bulg. J. Plant Physiol. 28: 57–67, 2002.
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Lazarova, D., Stanoeva, D., Popova, A. et al. UV-B induced alteration of oxygen evolving reactions in pea thylakoid membranes as affected by scavengers of reactive oxygen species. Biol Plant 58, 319–327 (2014). https://doi.org/10.1007/s10535-014-0402-0
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DOI: https://doi.org/10.1007/s10535-014-0402-0