Abstract
The influence of UV-B irradiation on photosynthetic oxygen evolution by isolated spinach thylakoids has been investigated using thermoluminescence measurements. The thermoluminescence bands arising from the S2QB - (B band) and S2QA − (Q band) charge recombination disappeared with increasing UV-B irradiation time. In contrast, the C band at 50°C, arising from the recombination of QA - with an accessory donor of Photosystem II, was transiently enhanced by the UV-B irradiation. The efficiency of DCMU to block QA to QB electron transfer decreased after irradiation as detected by the incomplete suppression of the B band by DCMU. The flash-induced oscillatory pattern of the B band was modified in the UV-B irradiated samples, indicating a decrease in the number of centers with reduced QB. Based on the results of this study, UV-B irradiation is suggested to damage both the donor and acceptor sides of Photosystem II. The damage of the water-oxidizing complex does not affect a specific S-state transition. Instead, charge stabilization is enhanced on an accessory donor. The acceptor-side modifications decrease the affinity of DCMU binding. This effect is assumed to reflect a structural change in the QB/DCMU binding site. The preferential loss of dark stable QB - may be related to the same structural change or could be caused by the specific destruction of reduced quinones by the UV-B light.
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Abbreviations
- Chl:
-
chlorophyll
- DCMU:
-
3-(3,4,-dichlorophenyl)-1,1-dimethylurea
- PS II:
-
Photosystem II
- QA :
-
first quinone electron acceptor of PS II
- QB :
-
second quinone electron acceptor of PS II
- Tyr-D:
-
accessory electron donor of PS II
- S0-S4 :
-
charge storage states of the water-oxidizing complex
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Hideg, É., Sass, L., Barbato, R. et al. Inactivation of photosynthetic oxygen evolution by UV-B irradiation: A thermoluminescence study. Photosynth Res 38, 455–462 (1993). https://doi.org/10.1007/BF00046774
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DOI: https://doi.org/10.1007/BF00046774