Abstract
Feverfew (Tanacetum parthenium) is a medicinal plant belonging to the Asteraceae family. To improve understanding terpene metabolism in feverfew, the relative gene expression of four key genes coding 3-hydroxy-3-methylglutarylcoenzyme A reductase (HMGR) and germacrene A synthase (GAS) from the mevalonic acid pathway (MVA), as well as 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) and hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) from the methyl erythritol phosphate pathway (MEP), were examined. Target organs and tissues included young leaves (not fully expanded), mature leaves (fully expanded), flowers, stems, roots, and glandular trichomes. HMGR, DXR, and HDR were isolated and sequenced for the first time in feverfew. Real-time quantitative PCR analysis revealed differential expression of these genes in feverfew tissues and developmental stages.
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Abbreviations
- DMADP:
-
dimethylallyl diphosphate
- DXP:
-
1-deoxy-D-xylulose-5-phosphate
- DXR:
-
1-deoxy-D-xylulose- 5-phosphate reductoisomerase
- FDP:
-
farnesyl diphosphate
- GAS:
-
germacrene A synthase
- HDR:
-
hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase
- HMGR:
-
3-hydroxy-3-methylglutaryl-coenzyme A reductase
- IDP:
-
isopentenyl diphosphate
- MEP:
-
methylerythritol phosphate
- MVA:
-
mevalonic acid
- ORF:
-
open reading frame
- RACE:
-
rapid amplification of cDNA ends
- RGE:
-
relative gene expression
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Acknowledgements: This work was financially supported by the University of Kurdistan and the Iran National Science Foundation. We gratefully thank Claudia Vickers (the Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Australia) for reading the manuscript and useful comments. Jafar Abdollahzadeh (the Department of Plant Protection, Faculty of Agriculture, University of Kurdistan, Iran) is acknowledged for phylogenetic analysis.
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Majdi, M., Karimzadeh, G. & Malboobi, M.A. Spatial and developmental expression of key genes of terpene biosynthesis in Tanacetum parthenium . Biol Plant 58, 379–384 (2014). https://doi.org/10.1007/s10535-014-0398-5
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DOI: https://doi.org/10.1007/s10535-014-0398-5