Abstract
Batch fermentation by Streptomyces pristinaespiralis with the addition of adsorbent resins was used to increase the production of pristinamycin. In consideration of the adsorption capacity and the desorption ability, a polymeric resin, JD-1, was finally selected. The maximum production of pristinamycin in Erlenmeyer flasks went up to 1.13 from 0.4 g l−1, by adding 12% (w/v) resin JD-1 into the culture broth at 20 h after inoculation. In a 3 l bioreactor, pristinamycin fermentation with the addition of 12% (w/v) resin JD-1 at 20 h after inoculation reached 0.8 g l−1, which was a 1.25-fold increase over fermentation without resin.
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Acknowledgements
The work was financially supported by National Natural Science Foundation of China (Grant No. 20576122), Department of Science and Technology, Zhejiang Province, China (Grant No. 2004C13007) and Natural Science Foundation of Ningbo City, China (Grant No. 2005A610018).
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Jia, B., Jin, ZH., Lei, YL. et al. Improved production of pristinamycin coupled with an adsorbent resin in fermentation by Streptomyces pristinaespiralis . Biotechnol Lett 28, 1811–1815 (2006). https://doi.org/10.1007/s10529-006-9157-9
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DOI: https://doi.org/10.1007/s10529-006-9157-9