Abstract
A modified procedure of cDNA-amplified fragment length polymorphism (cDNA-AFLP) combined with silver staining is described which generates clear band patterns from both fungal total RNA and poly-A RNA. The silver staining procedure takes only 25 min. Some 42–55 transcript-derived fragments (TDFs) were yielded from the total RNA of eight fungal pathogens. In comparison with the fingerprints generated from Mycosphaerella graminicola total RNA, those from poly-A RNA showed eight TDFs with an increased intensity and also seven additional TDFs. It suggests that poly-A RNA is preferable as starting material for ideal cDNA-AFLP analysis.
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Received 21 September 2005; Revisions requested 27 September 2005; Revisions received 21 November 2005; Accepted 23 November 2005
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Guo, JR., Schnieder, F. & Verreet, J.A. Differences Between the Fingerprints Generated from Total RNA and Poly-A RNA using a Modified Procedure of cDNA-AFLP and Silver Staining. Biotechnol Lett 28, 267–270 (2006). https://doi.org/10.1007/s10529-005-5529-9
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DOI: https://doi.org/10.1007/s10529-005-5529-9