Abstract
A thraustochytrid-like microorganism (strain 12B) was isolated from the mangrove area of Okinawa, Japan. On the basis of its ectoplasmic net structure and biflagellate zoospores we determined strain 12B to be a novel member of the phylum Labyrinthulomycota in the kingdom Protoctista. When grown on glucose/seawater at 28 °C, it had a lipid content of 58% with docosahexaenoic acid (DHA; 22:6 n−3) at 43% of the total fatty acids. It had a growth rate of 0.38 h−1. The DHA production rate of 2.8 ± 0.7 g l−1 day−1 is the highest value reported for any microorganism.
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T Aki K Hachida K Yoshinaga Y Katai T Yamasaki S Kawamoto T Kakizono T Yamaoka S Shigeta O Suzuki K Ono (2003) ArticleTitleThraustochytrid as a potential source of carotenoids J. Am. Oil. Chem. Soc. 80 789–794 Occurrence Handle1:CAS:528:DC%2BD3sXmvFGlsro%3D
Barclay WR (1992) Process for the heterotrophic production of microbial products with high concentrations of omega−3 highly unsaturated fatty acids. United States Patent 5,130,242
J Bezard JP Blond A Bernard P Clouet (1994) ArticleTitleThe metabolism and availability of essential fatty acids in animal and human tissues Reprod. Nutr. Dev. 34 539–568 Occurrence Handle1:CAS:528:DyaK2MXktlSgsbg%3D Occurrence Handle7840871
EG Bligh WJ Dyer (1959) ArticleTitleA rapid method of total lipid extraction and purification Can. J. Biochem. Physiol. 37 911–917 Occurrence Handle1:CAS:528:DyaG1MXhtVSgt70%3D Occurrence Handle13671378
RC Gaver CC Sweeley (1965) ArticleTitleMethods for methanolysis of sphingolipids and direct determination of long-chain bases by gas chromatography J. Am. Oil. Chem. Soc. 42 294–298 Occurrence Handle1:STN:280:CCqD1czmvVA%3D Occurrence Handle14279115
I Gill R Valivety (1997) ArticleTitlePolyunsaturated fatty acids, part 1: occurrence, biological activities and applications Trends Biotechnol. 15 401–409 Occurrence Handle1:CAS:528:DyaK2sXmslCksL4%3D Occurrence Handle9351284
RH Haschemeyer RJ Meysers (1972) Negative staining MA Hayat (Eds) Principles and Techniques of Electron Microscopy NumberInSeriesVol. 2 Van Nostrand Reinhold Co New York 114–117
R Kroes EJ Schaefer RA Squire GM Williams (2003) ArticleTitleA review of the safety of DHA45-oil Food Chem. Toxicol. 41 1433–1446 Occurrence Handle1:CAS:528:DC%2BD3sXntVGnsrc%3D Occurrence Handle12962995
Kyle DJ, Reeb SE, Sicotte VJ (1995) Infant formula and baby food containing docosahexaenoic acid obtained from dinoflagellates. United States Patent 5,397,591
TE Lewis PD Nichols TA McMeekin (1999) ArticleTitleThe biotechnological potential of thraustochytrids Mar. Biotechnol. 1 580–587 Occurrence Handle10.1007/PL00011813 Occurrence Handle1:CAS:528:DC%2BD3cXktFCitw%3D%3D Occurrence Handle10612683
Y Orikasa A Yamada R Yu Y Ito T Nishida I Yumoto K Watanabe H Okuyama (2004) ArticleTitleCharacterization of the eicosapentaenoic acid biosynthesis gene cluster from Shewanella sp. strain SCRC-2738 Cell Mol. Biol. 50 625–630 Occurrence Handle1:CAS:528:DC%2BD2cXhtVSis7fO Occurrence Handle15565743
D Porter (1989) Phylum Labyrinthulomycota L Margulis (Eds) Handbook of Protoctists Jones & Bartlett Publishers Boston 388–398
C Ratledge (2004) ArticleTitleFatty acid biosynthesis in microorganisms being used for single cell oil production Biochimie 86 807–815 Occurrence Handle10.1016/j.biochi.2004.09.017 Occurrence Handle1:CAS:528:DC%2BD2cXhtVKksLbK Occurrence Handle15589690
L Sijtsma ME de Swaaf (2004) ArticleTitleBiotechnological production and applications of the ω−3 polyunsaturated fatty acid docosahexaenoic acid Appl. Microbiol. Biotechnol. 64 146–153 Occurrence Handle10.1007/s00253-003-1525-y Occurrence Handle1:CAS:528:DC%2BD2cXis1Gls7c%3D Occurrence Handle14740186
A Singh OP Ward (1996) ArticleTitleProduction of high yields of docosahexaenoic acid by Thraustochytrium roseum ATCC 28210 J. Ind. Microbiol. 16 370–373 Occurrence Handle10.1007/BF01570118 Occurrence Handle1:CAS:528:DyaK28XkslSksrg%3D
ME Swaaf Particlede TC Rijk Particlede P Meer Particlevan der G Eggink L Sijtsma (2003) ArticleTitleAnalysis of docosahexaenoic acid biosynthesis in Crypthecodinium cohnii by 13C labeling and desaturase inhibitor experiments J. Biotechnol. 103 21–29 Occurrence Handle12770501
N Takakuwa M Kinoshita Y Oda M Ohnishi (2002) ArticleTitleExistence of cerebroside in Saccharomyces kluyveri and its related species FEMS Yeast Res. 2 533–538 Occurrence Handle1:CAS:528:DC%2BD38XovFGgsbs%3D Occurrence Handle12702269
R Vazhappilly F Chen (1998) ArticleTitleHeterotrophic production potential of omega−3 polyunsaturated fatty acids by microalgae and algae-like microorganisms Botanica Marina 41 553–558 Occurrence Handle1:CAS:528:DyaK1cXnvVahtL8%3D
T Yaguchi S Tanaka T Yokochi T Nakahara T Higashihara (1997) ArticleTitleProduction of high yields of docosahexaenoic acid by Schizochytrium limacinum strain SR21 J. Am. Oil. Chem. Soc. 74 1431–1434 Occurrence Handle1:CAS:528:DyaK2sXnsFGrtr8%3D
T Yokochi D Honda T Higashihara T Nakahara (1998) ArticleTitleOptimization of docosahexaenoic acid production by Schizochytrium limacinum SR21 Appl. Microbiol. Biotechnol. 49 72–76 Occurrence Handle10.1007/s002530051139 Occurrence Handle1:CAS:528:DyaK1cXhtVantbc%3D
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Received 7 October 2005; Revisions requested 7 October 2005; Revisions received 15 November 2005; Accepted 15 November 2005
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Perveen, Z., Ando, H., Ueno, A. et al. Isolation and Characterization of a Novel Thraustochytrid-like Microorganism that Efficiently Produces Docosahexaenoic Acid. Biotechnol Lett 28, 197–202 (2006). https://doi.org/10.1007/s10529-005-5335-4
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DOI: https://doi.org/10.1007/s10529-005-5335-4