Abstract
The BhMIR32 xyn11A gene, encoding an extracellular endoxylanase of potential interest in bio-bleaching applications, was amplified from Bacillus halodurans MIR32 genomic DNA. The protein encoded is an endo-1,4-β-xylanase belonging to family 11 of glycosyl hydrolases. Its nucleotide sequence was analysed and the mature peptide was subcloned into pET22b(+) expression vector. The enzyme was over-expressed in a high density Escherichia coli culture as a soluble and active protein, and purified in a single step by immobilised metal ion affinity chromatography with a specific activity of 3073 IU mg−1.
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Acknowledgments
The authors gratefully acknowledge to Profs. Rajni Hatti-Kaul and Bo Mattiasson. This work was supported by CONICET, Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina) and by the Swedish International Development Cooperation Agency (SIDA).
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Martínez, M.A., Delgado, O.D., Baigorí, M.D. et al. Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans. Biotechnol Lett 27, 545–550 (2005). https://doi.org/10.1007/s10529-005-2879-2
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DOI: https://doi.org/10.1007/s10529-005-2879-2