Abstract
To confer the ability to ferment cello-oligosaccharides on the ethanol-producing bacterium, Zymomonas mobilis, the β-glucosidase gene from EmRuminococcus albus, tagged at its N-terminal with the 53-amino acid Tat signal peptide from the periplasmic enzyme glucose–fructose oxidoreductase from Z. mobilis, was introduced into the strain. The tag enabled 61% of the β-glucosidase activity to be transported through the cytoplasmic membrane of the recombinant strain which then produced 0.49 g ethanol/g cellobiose.
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Revisions requested 9 November 2004; Revisions received 10 December 2004; Accepted 13 December 2004
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Yanase, H., Nozaki, K. & Okamoto, K. Ethanol production from cellulosic materials by genetically engineered Zymomonas mobilis . Biotechnol Lett 27, 259–263 (2005). https://doi.org/10.1007/s10529-004-8295-1
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DOI: https://doi.org/10.1007/s10529-004-8295-1