Abstract
An indirect immunoassay for quantitative determination of ampicillin (range, 10–1000 ng/ml) in buffer or milk has been developed. Polyclonal antibodies were obtained against ampicillin conjugated with bovine serum albumin; the conjugate was synthesized by direct condensation using carbodiimide. The antibodies were specific for ampicillin and exhibited low cross-reactivity to other penicillins (azlocillin, 17%; penicillin G, 10%; piperacillin, 5%; and carbenicillin, 4%). Matrix effects were minimized by combining the use of a casein-supplemented buffer (content of casein, 1%) with sample dilution. Limit of detection for ampicillin in milk (diluted tenfold) was equal to 5.0 ng/ml (which corresponded to 50 ng/ml of the original sample).
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Translated from Prikladnaya Biokhimiya i Mikrobiologiya, Vol. 41, No. 6, 2005, pp. 668–675.
Original Russian Text Copyright © 2005 by Samsonova, Shchelokova, Ivanova, Rubtsova, Egorov.
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Samsonova, Z.V., Shchelokova, O.S., Ivanova, N.L. et al. Enzyme-Linked Immunosorbent Assay of Ampicillin in Milk. Appl Biochem Microbiol 41, 589–595 (2005). https://doi.org/10.1007/s10438-005-0107-4
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DOI: https://doi.org/10.1007/s10438-005-0107-4