Abstract
A range of species of four mixed bacterial cultures was studied by molecular systematics methods with the use of 16S rRNA genes. The cultures had been developed for application in minireactors, to degrade volatile organic compounds (VOCs): ethyl benzene, m-xylene, styrene, and o-xylene. A sample of 30 plasmid rDNA clones was obtained for each of the mixed cultures. The clones were analyzed by RFLP according to two restriction sites. Major variants of the 16S-rDNA sequences, corresponding to the most abundant species, were determined for each association. Sequencing of four clones of predominant 16S-rDNAs showed that the culture consuming ethyl benzene was dominated by Pseudomonas fluorescens; o-xylene, by Achromobacter xylosoxydans; styrene, by Pseudomonas veronii; and m-xylene, by Delftia acidovorans. Minor components of all four cultures were generally similar. They included species of the genera Sphingobacter, Rhizobium, Mesorhizobium, Pedobacter, and Paenibacillus. Sampling sequencing of genes for 16S rRNA cloned from total genomic DNA allowed quantitative determination of the composition of actual bacterial associations consuming VOCs in minireactors.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
REFERENCES
Utkin, I.B., Yakimov, M.M., Kozlyak, E.I., and Rogozhin, I.S., Prikl. Biokhim. Mikrobiol., 1991, vol. 27, no.6, pp. 785–803.
Bezborodov, A.M., Rogozhin, I.S., Ushakova, N.A., Kurlovich, A.E., Zagustina, N.A., and Popov, V.O., Prikl. Biokhim. Mikrobiol., 1998, vol. 34, no.2, pp. 265–269.
RF Patent no. 2090246, 1997.
Zhukov, V.G., Rogozhin, I.S., Ushakova, N.A., Zagustina, N.A., Popov, V.O., and Bezborodov, A.M., Prikl. Biokhim. Mikrobiol., 1998, vol. 34, no.4, pp. 370–376.
Maniatis, T., Sambrook, J., and Fritsch, E.F., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor: Cold Spring Harbor Laboratory, 1989.
Anzai, Y., Kim, H., Park, J.-Y., Wakabayashi, H., and Oyaizu, H., Int. J. Syst. Evol. Microbiol., 2000, vol. 50, no.7, pp. 1563–1589.
Ficker, M., Krastel, K., Orlicky, S., and Edwards, E., Appl. Environ. Microbiol., 1999, vol. 65, no.12, pp. 5576–5585.
Roy, S., Gendron, J., Delhomenie, M.-C., Bibeau, L., Heintz, M., and Brzezinski, R., Appl. Environ. Microbiol., 2003, vol. 61, no.1, pp. 366–373.
Greene, E.A., Kay, J.G., Jaber, K., Stehmeier, L.G., and Voordouw, G., Appl. Environ. Microbiol., 2000, vol. 66, no.12, pp. 5282–5289.
Oerther, D.B., Pernthaler, J., Schramm, A., Amann, R., and Raskin, L., Appl. Environ. Microbiol., 2000, vol. 66, no.5, pp. 2154–2165.
Christensen, B.B., Haagensen, J.-A.J., Heydorn, A., and Molin, S., Appl. Environ. Microbiol., 2000, vol. 68, no.5, pp. 2495–2502.
Stoffels, M., Amann, R., Ludwig, W., Hekmat, D., and Schleifer, K.-H., Appl. Environ. Microbiol., 1998, vol. 64, no.3, pp. 930–939.
Moller, S., Sternberg, C., Andersen, J., Christensen, B.-B., Ramos, J.-L., Givskov, M., and Molin, S., Appl. Environ. Microbiol., 1998, vol. 64, no.2, pp. 721–732.
Moller, S., Pedersen, A.-R., Poulsen, L.K., Arvin, E., and Molin, S., Appl. Environ. Microbiol., 1996, vol. 62, no.12, pp. 4632–4640.
Author information
Authors and Affiliations
Additional information
Translated from Prikladnaya Biokhimiya i Mikrobiologiya, Vol. 41, No. 2, 2005, pp. 176–184.
Original Russian Text Copyright © 2005 by Khomenkov, Shevelev, Zhukov, Kurlovich, Zagustina, Popov.
Rights and permissions
About this article
Cite this article
Khomenkov, V.G., Shevelev, A.B., Zhukov, V.G. et al. Application of molecular systematics to study of bacterial cultures consuming volatile organic compounds. Appl Biochem Microbiol 41, 154–161 (2005). https://doi.org/10.1007/s10438-005-0026-4
Received:
Issue Date:
DOI: https://doi.org/10.1007/s10438-005-0026-4