Abstract
This work for the first time describes a centrifugal technique for the production and manipulation of highly monodisperse water droplets (CV of droplet diameter below 2%) immersed in a continuous flow of immiscible oil. Within a given working range, droplet volumes (5–22 nL) and their mutual spacing is governed by the channel geometry and the frequency of rotation. Different regimes of liquid–liquid flows are presented. We also demonstrate capabilities like droplet splitting and sedimentation as well as the production of two colored droplets, thus setting the stage for a novel centrifugal platform for multiphase flows.
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1 Introduction
Emulsions are thermodynamically unstable multiphase systems consisting of at least two immiscible liquids. Within an emulsion, small droplets of the internal or discontinuous phase are dispersed throughout the other, so-called external or continuous phase. Despite their principal instability, emulsions can be set in metastable state where the droplets retain their integrity for extended periods of time if their phase interface is supported by surfactants. The production of emulsions constitutes an essential part of a large number of process engineering applications within the production chain of food, cosmetics, or pharmaceuticals. With the advent of microfluidic systems, droplet emulsions are also considered a promising candidate for analytical systems. As the size of the droplets often plays a vital role in the application, it is essential to accurately control the droplet dimensions and volume fractions within the multiphase system.
Standard processes use high shear forces to generate droplets, e.g. by rotor-stator, ultrasonic or high-pressure homogenizing systems. However, as the fundamental droplet formation mechanism is of statistical nature, these methods lead to broad droplet-size distributions. Another disadvantage of these conventional production techniques is the parasitic heat induced into the multiphase system which may, for instance, interfere with sensible pharmaceutical substances.
Droplets can also be generated by the surface tension controlled break-off issued from tiny orifices. In this case the droplet size is tightly correlated to the cross section of the orifice. Ideally, a constant flow rate thus allows to produce a highly monodisperse train of droplets. Following this scheme, several microfluidic methods for the production of droplets have been presented (Thorsen et al. 2001; Ganan-Calvo and Gordillo 2001; Anna et al. 2003; Sugiura et al. 2001; Link et al. 2004; Hessel et al. 2002). The controlled manipulation of droplets in the confining rigid boundaries of microchannels can also be applied for additional unit operations in droplet processing like splitting, merging, or separation. We here investigate the formation and the controlled manipulation of liquid–liquid flows on our recently introduced centrifugal microprocess engineering platform (Haeberle et al. 2005a, b, c; Ducrée et al. 2004, 2006).
This paper is structured as follows. Section 2 describes the physics of centrifugal droplet generation and its difference to pressure driven mechanisms. In Sect. 3, the mechanism of droplet formation in a centrifugal driven flow-focusing structure is presented. Section 4 describes the different types of multiphase flows realized with this approach and important unit operations for droplet processing like splitting of droplets, droplet sedimentation, and the production of biphasic droplets.
2 Physics of centrifugal droplet generation
In the here presented centrifugal multiphase flow platform (Haeberle et al. 2005d), liquid propulsion results from the centrifugal pseudoforce generated by the rotary motion of a microfluidic channel network. Considering a static case of a liquid plug resting within the rotating frame of reference, an equivalent pressure can be derived at the radial outer end of the plug, scaling with the square of the rotational frequency ω (Brenner et al. 2005). However, the hydrodynamics within a centrifugal liquid flow deviates significantly from pressure driven flows. In the following, two differences are highlighted that are of particular importance for multiphase liquid flows.
First, the centrifugal force represents a body force acting on each fluid volume element while the propulsion by an external pressure difference is transmitted by the surface. This, for instance, impedes the centrifugal pumping of gases due to their low density while enabling the active “falling” of particles or droplets throughout a carrier liquid of lower density.
Secondly, the centrifugally induced “artificial gravity” linearly increases with the radial distance from the center, leading to an internal pressure distribution in a liquid flow which settles below the (atmospheric) pressure at the open inlet and outlet of the channel (Kim and Kwon 2006). This unique pressure distribution eases the requirements on the sealing of the microchannels, allowing very high flow rates and throughputs (Haeberle et al. 2005).
A characteristic distribution of the relative pressure along the center of a microchannel (length 3 cm, width 300 μm, height 200 μm, radial position of the inlet 2 cm) for a pressure as well as a centrifugal driven flow is depicted in Fig. 1 (CFD-ACE+, ESI CFD, Inc., Huntsville, AL, USA). The volumetric flow rate of 172 μL s−1 of the simulated centrifugal flow for ω = 377 rad s−1 is imposed as an inlet boundary condition in the pressure driven flow. The two pressure distributions display a strikingly different character: the relative pressure distribution of the pressure driven flow exhibits a maximum at the inlet of approximately 1 bar. However, the relative pressure of the centrifugal flow coincides with the ambient pressure at the “open” inlet and outlet of the channel, respectively. In between, the relative pressure becomes negative (neglecting entrance effects).
These distinct centrifugal hydrodynamic conditions imply characteristic deviations regarding the droplet generation principles compared to a pressure-driven flow. For example, the issuing of gas bubbles into a continuous liquid flow at a flow-focusing junction has been intensively investigated in pressure driven systems (Garstecki et al. 2004, 2005). Evidently, this approach cannot directly be implemented on the centrifugal platform since the buoyancy prohibits a concurrent centrifugal propulsion of the gas phase in the continuous liquid phase, thus requiring an independent pressure source for the gas on the centrifugal platform (Haeberle et al. 2006).
The basic impact parameters governing the centrifugally induced droplet formation process at the junction of a flow-focusing structure (Anna et al. 2003) under rotation are depicted in Fig. 2. The two pulling forces, namely the centrifugal force (density) F ω and the hydrodynamic drag F d of the sheath flows φ o expel the water flow out of the central channel. The break-off of the water droplets is stimulated by the geometric constriction right after the junction as well as the inertia associated with the transversal component of the oil flows (F i).
The centrifugal force depends on the density difference of the two phases, the radial position of the nozzle and the square of the rotational frequency ω. The magnitude of the constricting oil flow depends on the radial position, the radial height, and the hydrodynamic resistance of the oil guiding channels as well as the square of ω.
The interfacial tension force F σ of the emerging liquid bridge between the droplet and the central inlet channel is the counterforce opposing the droplet break-off. F σ scales with the interfacial tension σ between the water and oil phase as well as the (minimum) circumference of the bridge indicated by the white line in Fig. 2 (assuming no bending along the channel height). During the droplet formation process, the overall interfacial area of the plug exposed to the focusing oil flow expands and thus also the impact of the hydrodynamic drag F d and inertial forces F i of the constricting oil flows φ o. F i tends to squeeze the interface back into the issuing nozzle until the plug exceeds a critical dimension. As soon as the plug is long enough, however, F i restricts the circumference of the bridge and therefore supports the droplet break-off.
The opposing pulling and interfacial force depend on the transient shape and volume of the evolving liquid plug and thus the droplet break-off takes place as soon as the plug exceeds a critical mass for a given (minimum) circumference of the liquid bridge. Since both the flow velocity of the plug as well as the critical mass depend on the frequency of rotation ω, the droplet generation frequency f as well as the droplet size (diameter d, projected surface area A) can be manipulated by changing ω. The droplet generation process on the centrifugal multiphase platform can therefore be described as a dripping nozzle in a frequency controlled artificial gravity field zω 2, assisted by symmetric outer strangulating and sweeping oil flows φ o. The coordinate z denotes the radial position from the center of rotation, amounting to about 3 cm in our system.
Following these hydrodynamic considerations, the diameter of the droplets d should shrink towards decreasing cross section of the junction as well as towards increasing oil flow rate φ o and centrifugal body force F ω . Also the droplet generation frequency f is supposed to increase with φ o and F ω . Since both, φ o and F ω , scale with ω 2, the rotational frequency ω acts as a dynamically adjustable setscrew for an online control of the droplet size d and droplet formation frequency f.
A dimensionless number that expresses the scaling of effects related to the viscous shear stress with respect to the interfacial tension (Link et al. 2004; Joanicot and Ajdari 2005) is the capillary number
with the viscosity of the continuous phase η, the interfacial tension between the two phases σ, and the velocity of the dispersed phase out of the channel into the junction area u (Tan et al. 2004).
Different modes of droplet formation are observed for different capillary numbers, Ca. For low Ca (< 1), droplets are formed directly at the junction of the structure. This droplet formation mechanism is referred to as the “dripping” regime (Utada et al. 2005). Towards large Ca, the “jetting regime” is entered where axially extended plugs of the dispersed liquid are injected into the continuous phase (Cramer et al. 2004). These jets are poorly stable and tend to break-off into droplets upon transient fluctuations within the jet (Rayleigh instability, Lord Rayleigh 1878). Due to the statistical nature of such a break-off, a broad droplet-size distribution is observed (Utada et al. 2005). As this work aims at the production of highly monodisperse droplet trains, the “dripping” mode is the method of choice.
However, the capillary number Ca does not take directly into account the special conditions in the centrifugal “artificial gravity” field zω² as well as the dimensions of the orifice at which the droplet breaks up into the continuous phase. The impact of the artificial gravity on the droplet formation is accounted by the Bond number
representing the ratio of centrifugal and interfacial effects on the droplet. ρ d describes the density difference between the two phases and L the characteristic length scale, i.e. L² the cross section of the issuing nozzle.
With typical angular frequencies of ω = 2πν = 100 rad s−1, common values of the centrifugally induced artificial gravity a range in the order of several 100 m s−2 with the gravitational acceleration g = 9.81 m s−2, i.e., a 10-fold increase of g (Eq. 2). Towards elevated frequencies ω, Bo increases and the impact of the surface tension reduces to promote the centrifugally induced “drawing” of jets from the nozzle. For a given set of liquids, dripping is thus linked to small capillary numbers Ca ∼ u ∼ ω²L 2 (Ducree et al. 2005) as well as small Bond numbers Bo ∼ aL² ∼ ω²L². As both, Ca as well as Bo, scale with ω²L 2, the dripping regime is favored by small cross sections L 2 of the issuing nozzles as well as small rotational frequencies ω.
Nevertheless, the capillary number Ca cannot be regarded as the primary control parameter for the droplet volume V d since it does not represent the exact details of the droplet formation mechanism in a microfluidic flow focusing geometry. The droplet volume V d in pressure driven flow focusing devices (FFD) has been described as a function of the flow rate Φ o and the viscosity η of the continuous as well as the pressure p within the dispersed phase at the junction (V d ∝ p /Φ o η) (Garstecki et al. 2004, 2005).
In the centrifugal driven FFD, the continuous flow rate Φ o as well as the pressure inside the dispersed phase p scale with ω 2 and thus V d ought to be independent of the frequency of rotation ω. However, our experimental investigations will reveal a change of the droplet diameter with ω. We consider the additional centrifugal volume force F ω resulting in a net force pulling the (more dense) dispersed phase out of the central channel as the main reason for that dependence, thus disclosing the different hydrodynamic nature of the droplet formation mechanisms in centrifugal and pressure driven systems.
3 Centrifugal emulsification
3.1 Channel design and system setup
The channel layout exhibits an inlet for the dispersed phase Φ w which is enclosed at an angle α by two outer “sheath” flows Φ o (Fig. 3). Downstream the central junction, the dispersed phase Φ w is guided by the sheath flows into the common channel carrying the emulsion within a given working range of flow conditions. Subsequently, the multiphase flow is driven along the droplet channel for further processing or characterization. The influence of the channel network on the flow rates in the inlet and outlet channels is quantified by their individual hydrodynamic resistances R o, R w of the inlet channels and the outlet channel R out.
The flow-focusing structure has been adapted to the needs of the centrifugal microfluidic approach. Therefore, the entire channel layout is arranged symmetrical to the radial droplet channel and placed on a disk-shaped module rotating at a frequency ω about the center of the disk. The characteristics of the produced emulsion, i.e., the droplet cross section A, the droplet diameter d, and the drop-to-drop spacing Δ, are measured optically (Grumann et al. 2005). A, d, and Δ can be influenced by the design parameters, mostly the flow resistances R o, R w, and R out as well as the frequency of rotation ω.
All microfluidic channel structures within this paper have been fabricated by high-precision milling into a cyclo-olefin copolymer (COC) substrate with a diameter of 115 mm and a thickness of 2 mm. The channels were sealed by another transparent copolymer foil, using a thermal diffusion bonding process. The contact angles of the used substances with the untreated polymer surface are listed in Table 1.
The complete setup comprises the microstructured polymer disk featuring the flow-focusing microchannels and rotating reservoirs that are mounted on top of the disk (Haeberle et al. 2005a). These reservoirs enable either the storage of a certain liquid volume on the rotating module (several mL) or the continuous injection of additional liquid during rotation. The collection of the multiphase flow after processing can also be realized in a batch-like fashion using onboard reservoirs rotating with the disk or in a continuous fashion by ejecting the liquid onto a stationary drainage ring.
Regarding the device, the main benefit of the centrifugal technology is the modular setup consisting of a robust actuation unit, e.g., a conventional centrifuge, and a passive rotating module without moving parts to process the liquids. The centrifugal propulsion of flow and thus the centrifugally stimulated break-off process then results from the pulse-free spinning motion of the rotor which is stabilized by its own angular momentum. Compared to conventionally pumped systems, the centrifugal principle tends to dampen high-frequency pressure perturbations by its inertial momentum, thus leading to an improved reproducibility of the break-off process.
3.2 Multiphase system
We investigate a suspension of DI water droplets in sunflower oil (W/O emulsion). The water is colored by 2 vol% ink to enhance the contrast of captured images. No additional surfactants have been added to the continuous phase to stabilize the emulsion since they reduce the interfacial tension and consequently also the required energy to form a droplet by shear stress. Therefore, the system without surfactant has the highest demand on the microfluidic structure in order to form a droplet and serves as a model system. Table 1 shows the properties of the water (W) and the oil (O) phase, respectively. The contact angle has been measured on a planar COC surface.
3.3 Droplet formation in the centrifugal field
Droplets are generated at the junction of the flow-focusing structure where two outer oil flows and an enclosed water column are contacted and then driven through the radial droplet channel. The stroboscopic picture sequence displayed in Fig. 4 shows the droplet formation process under rotation at this part of the structure (Grumann et al. 2005).
Oil exhibits superior wetting properties, i.e., a smaller contact angle θ within the microchannels made of COC (Table 1). Thus, the water plug has to be actively “pulled” out of the central channel by the centrifugal force F ω , i.e., a critical bond number Bo (Eq. 2) has to be surpassed by increasing the rotational frequency ω. To avoid jetting, the flow velocity u of the dispersed phase out of the central channel into the growing droplet has to be throttled by introducing an elevated hydrodynamic resistance of the central channel R w. As mentioned in Sect. 1, the throttling reduces the capillary number Ca (Eq. 1) in order to support the desired dripping.
Following the sequence in Fig. 4, the water plug moves towards the center of the focusing structure. With increasing length of the plug, its mass and the effective surface shaped by the interplay of the oil flows and the surface tension grows. As soon as the strangulation by this focusing arrangement and the “artificial gravity” of the centrifugal field on the growing droplet overcomes the surface tension, a droplet is formed and carried away by the ambient oil flow.
4 Generation and manipulation of multiphase flows
As described in the previous section, the droplet formation process can be controlled by the flow conditions at the junction, i.e., mainly by the flow velocities and their respective ratios. The flow velocities are statically defined by the channel resistances R o, R w, and R out and they can dynamically be adjusted by changing the angular frequency ω = 2πv (Haeberle et al. 2005b).
4.1 Droplet trains and segmented flow
In this section, we present three different structures for the production of water droplets in a continuous oil-flow (W/O emulsions). Additionally, their main characteristics, namely the distribution of the droplet diameters d, the spacing between the droplets Δ, and the droplet production rate f are studied. The droplet spacing Δ is connected to the ratio between the droplet generation frequency and the flow rate within the outlet channel, i.e. the ability of the system to remove the generated droplets from the junction area.
Depending on the droplet size, the generated multiphase flows in Fig. 5 can be categorized into three different types:
-
1.
Isolated droplet trains: The diameter of water droplets falls short of the channel height. Thus, the droplets retain a spherical shape minimizing the surface tension.
-
2.
Squeezed droplet trains: The (relaxed) diameter of the water droplets exceeds the channel height. This mode could be used to produce, e.g. nonspherical microparticles (Dendukuri et al. 2005).
-
3.
Segmented flow: The (relaxed) diameter of the water droplets is larger than the height and the width of the channel. This mode is of interest for analytical applications (Song et al. 2003; Shestopalov et al. 2004; Roach et al. 2005).
The channel structures for the production of these types of emulsions are all based on the earlier presented flow-focusing principle (Fig. 3). Only the hydrodynamic resistances of the water R w and oil R o channels as well the shape and resistance of the droplet channel R out are adjusted according to Table 2.
The hydrodynamic resistance of low-aspect-ratio microchannels with a length l, a width w, and a height h is given by
for a liquid with viscosity η (Geschke et al. 2004). In Table 2, the geometrical part R′ = R/12η = l/wh³ of the fluidic resistance R is listed to focus on the free design parameters of the flow-focusing structure.
Highly monodisperse droplet trains are generated within a certain window of rotational frequencies for all three emulsification structures (Fig. 5). At low frequencies of rotation, no droplets are formed since the water plug rests in the central channel or even withdraws due to the favored oil-wetting behavior within the channels (low Bond number, Bo). However, above a certain frequency threshold v low, a water plug protrudes into the junction region and breaks up into equally sized droplets within the working range of the structure.
The isolated structure produces the smallest droplets indicating the magnitude of the strangulating oil flow as one major parameter influencing the droplet size. The hydrodynamic resistance of the oil and the outlet channel are small in that design thus scaling up the oil flow through the flow-focusing structure (the cross section of the central channel is the same for all three structures: ∼ 340 × 110 μm2).
In Fig. 5, also the capillary numbers, Ca, are given for two structures within the working range. The values for Ca have been calculated using Eq. 1, identifying the droplet velocity in the droplet channel with the mean velocity of the dispersed phase into the junction area u. The low values of Ca indicate that the upper frequency limit v high of working range where separate droplets can still be discerned is not determined by the transition of the droplet generation effect from dripping to jetting (which occurs at even higher frequencies of rotation, see Fig. 6). Instead, the limitation is associated with the contact (Δ = d) and merging of the initially separate droplets which are not removed fast enough from the junction area by the continuous oil flow.
Two parameters, the characteristic droplet area A and the droplet spacing Δ, can directly be derived from the stroboscopic grayscale pictures. An image processing algorithm has been used to automatically determine A by changing the picture contrast and searching for the outline of the ink-darkened water droplet. The diameter of the droplets d is calculated by assuming round droplets.
This is certainly an approximation since the droplets are squeezed to an unknown extent between the upper and lower wall of the channel (depth ∼200 μm). Consequently, also the droplet volume can only be estimated by multiplying the characteristic area A with the measured channel depth.
Figure 7 shows the calculated droplet diameter d as well as the droplet spacing Δ of the squeezed and isolated droplets structures over the frequency of rotation ν. The squeezed-droplet structure displays a wider working range since the flow velocity within the droplet channel is higher and thus the droplets are removed faster from the junction to avoid contacting of the issued droplets. Towards increasing frequencies of rotation, the droplet generation rate grows while their size shrinks (Fig. 7). Above the upper limit of the working range v high, the droplets get in contact or even merge. This indicates the thermodynamic instability of the surfactant-free macro-emulsion displaying a droplet diameter in the range of several hundred micrometers (Windhab et al. 2005).
The high monodispersity of the droplet trains is depicted in Fig. 8. The figure displays the diameter of 100 droplets produced in the isolated-droplet structure at a rotational frequency of 12 Hz. The mean diameter \( \ifmmode\expandafter\bar\else\expandafter\=\fi{d} \) of 211.33 μm relates to a droplet volume of approx. 7.7 nL. An extremely small coefficient of variation (CV = standard deviation / mean) proves the high reproducible break-off process in the centrifugal flow focusing structure.
Another important parameter that cannot be directly derived from the stroboscopic picture sequences is the droplet generation frequency f. A special mode of operation of the emulsification structures is used to determine f by the number of droplets issued in a fixed time interval Δt. First, the rotational frequency ω is kept below the threshold frequency where no droplets are produced. Afterwards, ω is raised as fast as possible with our centrifuge to the desired value and droplets are ejected at the junction. The time period for the acceleration lasts approximately 0.45 s for the largest frequency step from 10 to 28 Hz since the maximum acceleration of the centrifuge amounts to 40 Hz s−1.
Now, the number of produced droplets n d,t and n d,t+Δt of two subsequent stroboscopic pictures can be evaluated (Fig. 9). Since only fully developed droplets are considered, this method leads to a certain discretization error. The delay Δt between two subsequent stroboscopic pictures is set by the known capture frequency of the stroboscopic camera to arrive at
for the droplet rate. The “droplet counting method” is limited by the minimum time delay between two pictures, the maximum display window of the optical setup and the radial velocity of the droplets within the droplet channel. These parameters restrict the possibility to count all produced droplets in the second picture because as soon as the droplet train leaves the display detail, the required information of the number of droplets cannot be determined.
As depicted in Fig. 9, the droplet generation frequency f ranges from several ten up to about 400 droplets per second. The throughput may appreciably be enhanced by operating several channels on one rotational module or even several modules as a vertical stack in parallel (Ducrée et al. 2004). Our platform thus allows the controlled production and processing of a small number of droplets as well as a fast production of monodisperse emulsions.
4.2 Droplet splitting
Besides the controlled production of droplets, also their manipulation is important to enable droplet-based fluidic applications. We first study the splitting of a droplet into several subdroplets of defined size. To this end, a second flow-focusing junction on an outer diameter of the droplet channel is used to hydrodynamically induce the splitting of droplets generated further upstream (Fig. 10).
Depending on the frequency of rotation, the droplet is again squeezed at the junction by two lateral oil flows Φ o. Below a critical frequency, the velocity of these flows and consequently also their constricting impact remains too small, and the droplets are just transiently squeezed and then relax back to its equilibrium shape right after the junction. Beyond the critical frequency of roughly 21 Hz for the presented channel layout in Fig. 10, the droplet is completely split at the junction, and two spatially separated droplets proceed in the downstream direction. A further increase of the frequency of rotation leads to such a reinforced flow focusing at the junction, even reproducibly stimulating the isolation of a satellite droplet.
4.3 Droplet sedimentation
Processing different immiscible liquids in multiphase flows also implies the simultaneous processing of fluids with different rheological properties in the same system. The emulsification process is also referred to as mixing as it can also be looked at as a first step to homogenize the phase distribution. We here investigate the reverse unmixing or separation process which constitutes an important unit operation within many applications. Our centrifugal platform enables simple separation methods based on density differences of the phases by an accelerated sedimentation in the centrifugal force field.
The ability to selectively control the different phases is shown with a flow-splitting structure (Fig. 11). The multiphase W/O-emulsion flow is split into two outlet channels. Owing to their slightly increased mass density (∼1%), the droplets are driven towards the outer wall within the guide channel. After the bending, the radially outwards pointing centrifugal force continues pressing them against the outer channel wall and consequently, the complete droplet train leaves the disk via the first outlet channel. The forced contact of the droplets with the channel walls under the extreme “artificial gravity” of the centrifugal field is a new droplet handling operation supplementing our centrifugal multiphase-fluidics platform.
4.4 Two-phase droplets
We produce two-phase droplets by enclosing two phases which are commonly guided to the junction of the flow-focusing structure into one droplet (Fig. 12). A structure to produce such two-phase droplets has been realized and tested with pure water and ink as the two phases to be enclosed in the droplet. The two dispersed phases are contacted in the central inlet channel prior to their injection into the junction of the flow focusing structure.
Bi-colored droplets break off at the junction and emerge into the outlet channel. The contacting and parallel flow of the two liquids in the central channel displayed tendencies to become unstable, e.g. by the displacement of one liquid by the other within the experiments, eventually leading to single-phase droplets. Thus, further improved structures are to be fabricated in the future to get more stable hydrodynamics at the junction.
The double-droplet capability opens several new areas of application. For example, regarding miscible liquids, mixing experiments within very small “reaction chambers” represented by the droplets can be accomplished (Song et al. 2003; Roach et al. 2005; Gunther et al. 2005; Zheng et al. 2003). Another application is the production of two-colored droplets followed by a polymerization step to fabricate bi-colored beads, e.g., for display technologies (Nisisako et al. 2004a, b).
5 Conclusion
Novel centrifugal microfluidic methods for the continuous production and manipulation of highly monodisperse trains of water droplets in an oil flow have been investigated. By changing the channel geometry and the frequency of rotation, the flow regime could be switched between monodisperse emulsions and segmented-flow and the droplet size and generation rate can be influenced. Key unit operations such as splitting of droplets, separation of droplets from the continuous phase, and the production of two-phase droplets are demonstrated.
We expect further benefits of porting multiphase flows to the centrifugal platform in the comparatively high discharge rates (Ducrée et al. 2004) as well as the facile integration of centrifugal droplet sedimentation, thus enabling novel applications in the fields of droplet-based analytics and microprocess engineering.
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Acknowledgements
The authors are grateful to the partial support by the German “Landesstiftung Baden-Württemberg gGmbH”.
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Haeberle, S., Zengerle, R. & Ducrée, J. Centrifugal generation and manipulation of droplet emulsions. Microfluid Nanofluid 3, 65–75 (2007). https://doi.org/10.1007/s10404-006-0106-7
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DOI: https://doi.org/10.1007/s10404-006-0106-7