Abstract
Objective
Several studies have demonstrated differences in migraine patients when performing 1H-MRS; however, no studies have performed 1H-MRS in migraine without aura (MwoA), the most common migraine subtype. The aim of this 1H-MRS study was to elucidate whether any differences could be found between MwoA patients and controls by performing absolute quantification.
Materials and methods
1H-MRS was performed in 22 MwoA patients and 25 control subjects. Absolute quantification was based on the phantom replacement technique. Corrections were made for T1 and T2 relaxation effects, CSF content, coil loading and temperature. The method was validated by phantom measurements and in vivo measurements in the occipital visual cortex.
Results
After calibration of the quantification procedure and the implementation of the required correction factors, measured absolute concentrations in the visual cortex of MwoA patients showed no significant differences compared to controls, in contrast to relative results obtained in earlier studies.
Conclusion
In this study, we demonstrate the implementation of quantitative in vivo 1H-MRS spectroscopy in migraine patients. Despite rigorous quantification, no spectroscopic abnormalities could be found in patients with migraine without aura.
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References
Lance JW, Goadsby PJ (1998) Mechanism and management of headache. Butterworth-Heinemann, Boston
Silberstein SD, Lipton RB, Goadsby PJ (1998). In: Goadsby PJ (ed) Headache in clinical practice, edn. Isis Medical Media, Oxford, pp 69–112
Olesen J, Tfelt-Hansen P, Welch KMA (2000) The headaches. Williams & Wilkins, Philadelphia
The International Headache Society Classification Subcommittee: (2004) The international classification of headache disorders 2. Cephalalgia 24(S1): 1–160
Stovner LJ, Zwart JA, Hagen K, Terwindt GM, Pascual J (2006) Epidemiology of headache in Europe. Eur J Neurol 13(4): 333–345
Edmeads J, Mackell JA (2002) The economic impact of migraine: an analysis of direct and indirect costs. Headache 42(6): 501–509
Leonardi M, Steiner TJ, Scher AT, Lipton RB (2005) The global burden of migraine: measuring disability in headache disorders with WHOs classification of functioning, disability and health (ICF). J Headache Pain 6(6): 429–440
Schoenen J (1994) Pathogenesis of migraine: the biobehavioural and hypoxia theories reconciled. Acta Neurol Belg 94(2): 79–86
Schoenen J (1998) Cortical electrophysiology in migraine and possible pathogenic implications. Clin Neurosci 5(1): 10–17
Welch KM, Levine SR, D’Andrea G, Helpern JA (1988) Brain pH in migraine: an in vivo phosphorus-31 magnetic resonance spectroscopy study. Cephalalgia 8(4): 273–277
Welch KM, Levine SR, D’Andrea G, Schultz LR, Helpern JA (1989) Preliminary observations on brain energy metabolism in migraine studied by in vivo phosphorus 31 NMR spectroscopy. Neurology 39(4): 538–541
Barbiroli B, Montagna P, Cortelli P, Martinelli P, Sacquegna T, Zaniol P, Lugaresi E (1990) Complicated migraine studied by phosphorus magnetic resonance spectroscopy. Cephalalgia 10(5): 263–272
Sacquegna T, Lodi R, De Carolis P, Tinuper P, Cortelli P, Zaniol P, Funicello R, Montagna P, Barbiroli B (1992) Brain energy metabolism studied by 31P-MR spectroscopy in a case of migraine with prolonged aura. Acta Neurol Scand 86(4): 376–380
Barbiroli B, Montagna P, Cortelli P, Funicello R, Iotti S, Monari L, Pierangeli G, Zaniol P, Lugaresi E (1992) Abnormal brain and energy metabolism shown by 31P magnetic resonance spectroscopy in patients affected by migraine with aura. Neurology 42(6): 1209–1214
Montagna P, Cortelli P, Monari L, Pierangeli G, Parchi P, Lodi R, Iotti S, Frassineti C, Zaniol P, Lugaresi E, Barbiroli B (1994) 31P-Magnetic resonance spectroscopy in migraine without aura. Neurology 44(4): 666–669
Uncini A, Lodi R, Di Muzio A, Silvestri G, Servidei S, Lugaresi A, Iotti S, Zaniol P, Barbiroli B (1995) Abnormal brain and muscle energy metabolism shown by 31P-MRS in familial hemiplegic migraine. J Neurol Sci 129(2): 214–222
Lodi R, Montagna P, Soriani S, Iotti S, Arnaldi C, Cortelli P, Pierangeli G, Patuelli A, Zaniol P, Barbiroli B (1997) Deficit of brain and skeletal muscle bioenergetics and low brain magnesium in juvenile migraine: an in vivo 31P magnetic resonance spectroscopy interictal study. Pediatr Res 42(6): 866–871
Boska MD, Welch KM, Barker PB, Nelson JA, Schultz L (2002) Contrasts in cortical magnesium, phospholipid and energy metabolism between migraine syndromes. Neurology 58(8): 1227–1233
Watanabe H, Kuwabara T, Ohkubo M, Tsuji S, Yuasa T (1996) Elevation of cerebral lactate detected by localized 1H-magnetic resonance spectroscopy in migraine during the interictal period. Neurology 47(4): 1093–1095
Sandor PS, Dydak U, Schoenen J, Kollias SS, Hess K, Boesiger P, Agosti RM (2005) MR-spectroscopic imaging during visual stimulation in subgroups of migraine with aura. Cephalalgia 25(7): 507–518
Sarchielli P, Tarducci R, Preciutti O, Gobbi G, Pelliccioli GP, Stipa G, Alberti A, Capocchi G (2005) Functional 1H-MRS findings in migraine patients with and without aura assessed interictally. Neuroimage 24(4): 1025–1031
Dichgans M, Herzog J, Freilinger T, Wilke M, Auer DP (2005) 1H-MRS alterations in the cerebellum of patients with familal hemiplegic migraine type 1. Neurology 64(4): 608–613
Jacob A, Mahavish K, Bowden A, Smith ET, Enevoldson P, White RP (2006) Imaging abnormalities in sporadic hemiplegic migraine on conventional MRI, diffusion and perfusion MRI and MRS. Cephalalgia 26(8): 1004–1009
Schulz UG, Blamire AM, Corkill RG, Davies P, Styles P, Rothwell PM (2007) Association between cortical metabolite levels and clinical manifestations of migrainous aura: an MR-spectroscopy study. Brain 130(Pt12): 3102–3110
Gu T, Ma XX, Xu YH, Xiu JJ, Li CF (2008) Metabolite concentration ratios in thalami of patients with migraine and trigeminal neuralgia measured with 1H-MRS. Neurol Res 30(3): 229–233
Macri MA, Garreffa G, Giove F, Ambrosini A, Guardati M, Pierelli F, Schoenen J, Colonnese C, Maraviglia B (2003) Cerebellar metabolite alterations detected in vivo by proton MR spectroscopy. Magn Reson Imaging 21(10): 1201–1206
Ma Z, Wang SJ, Li CF, Ma XX, Gu T (2008) Increased metabolite concentration in migraine rat model by proton MR spectroscopy in vivo and ex vivo. Neurol Sci 29(5): 337–342
Grimaldi D, Tonon C, Cevoli S, Pierangeli G, Malucelli E, Rizzo G, Soriani S, Montagna P, Barbiroli B, Lodi R, Cortelli P (2010) Clinical and neuroimaging evidence of interictal cerebellar dysfunction in FHM2. Cephalalgia 30(5): 552–559
Naressi A, Couturier C, Castang I, de Beer R, Graveron-Demilly D (2001) Java-based graphical user interface for MRUI, a software package for quantitation of in vivo/medical magnetic resonance spectroscopy signals. Comput Biol Med 31(4): 269–286
Laudadio T, Mastronardi N, Vanhamme L, Van Hecke P, Van Huffel S (2002) Improved Lanczos algorithms for blackbox MRS data quantitation. J Magn Reson 157(2): 292–297
Vanhamme L, van den Boogaart A, Van Huffel S (1997) Improved method for accurate and efficient quantification of MRS data with use of prior knowledge. J Magn Reson 129(1): 35–43
Cavassila S, van Ormondt D, Graveron-Demilly D (2001) Cramer-rao bound analysis of spectroscopic signal processing methods. In: Yan H (eds) Signal processing for magnetic resonance imaging and spectroscopy, edn. Marcel Dekker, New York, pp 613–640
Tofts PS (2004) Spectroscopy: 1H metabolite concentrations. In: Tofts P (eds) Quantitative MRI of the brain: measuring changes caused by disease. John Wiley, Chichester, pp 299–340
Drost DJ, Riddle WD, Clarke GDAAPM MR Task Group #9: (2002) Proton magnetic resonance spectroscopy in the brain: report of AAPM MR Task Group #9. Med Phys 29(9): 2177– 2197
Kreis R (2004) Issues of spectral quality in clinical 1H magnetic resonance spectroscopy and a gallery of artifacts. NMR Biomed 17(6): 361–381
Tofts PS (1994) Standing waves in uniform water phantoms. J Magn Reson B 104(2): 143–147
Insko EK, Bolinger L (1993) Mapping of the rafiofrequency field. J Magn Reson A 103(1): 82–85
Helms G (2008) The principles of quantification applied to in vivo proton MR spectroscopy. Eur J Radiol 67(2): 218–229
Kreis R (1997) Quantitative localized 1H MR spectroscopy for clinical use. Prog Nucl Mag Res Sp 31: 155–195
Hennig J, Pfister H, Ernst T, Ott D (1992) Direct absolute quantification of metabolites in the human brain with in vivo localized proton spectroscopy. NMR Biomed 5(4): 193–199
Soher BJ, van Zijl PC, Duyn JH, Barker PB (1996) Quantitative proton MR spectroscopic imaging of the human brain. Magn Reson Med 35(3): 356–363
Helms G (2000) A precise and user-independent quantification technique for regional comparison of single volume proton MR spectroscopy of the human brain. NMR Biomed 13(7): 398–406
Hoult DI, Richards RE (1976) The signal-to-noise ratio of the nuclear magnetic resonance experiment. J Magn Reson 24(1): 71–85
Ernst T, Kreis R, Ross BD (1993) Absolute quantitation of water and metabolites in the human brain. I. Compartments and water. J Magn Reson B 102(1): 1–8
Lynch J, Peeling J, Auty A, Sutherland GR (1993) Nuclear magnetic resonance study of cerebrospinal fluid from patients with multiple sclerosis. Can J Neurol Sci 20(3): 194–198
Hetherington HP, Pan JW, Mason GF, Adams D, Vaughn MJ, Twieg DB, Pohost GM (1996) Quantitative 1H spectroscopic imaging of human brain at 4.1 T using image segmentation. Magn Reson Med 36(1): 21–29
Helms G (2003) T2-based segmentation of periventricular paragraph sign volumes for quantification of proton magnetic paragraph sign resonance spectra of multiple sclerosis lesions. Magn Reson Mater Phy 16(1): 10–16
Connelly A, Jackson GD, Duncan JS, King MD, Gadian DG (2004) Magnetic resonance spectroscopy in temporal lobe epilepsy. Neurology 44(8): 1411–1417
Lundbom N, Gaily E, Vuori K, Paetau R, Liukkonen E, Rajapakse JC, Valanne L, Hakkinen AM, Granstrom ML (2001) Proton spectroscopic imaging shows abnormalities in glial and neuronal cell pools in frontal lobe epilepsy. Epilepsia 42(12): 1507–1514
Mathews VP, Barker PB, Blackband SJ, Chatham JC, Bryan RN (1995) Cerebral metabolites in patients with acute and subacute strokes: concentrations determined by quantitative proton MR spectroscopy. AJR Am J Roentgenol 165(3): 633–638
Chang L, Ernst T, Tornatore C, Aronow H, Melchor R, Walot I, Singer E, Conford M (2001) Metabolite abnormalities in progressive multifocal leukoencephalopathy by proton magnetic resonance spectroscopy. Neurology 48(4): 836–845
Mlynarik V, Gruber S, Moser E (2001) Proton T1 and T2 relaxation times of human brain metabolites at 3 Tesla. NMR Biomed 14(5): 325–331
Schirmer T, Auer DP (2000) On the reliability of quantitative clinical magnetic resonance spectroscopy of the human brain. NMR Biomed 13(1): 28–36
Ozdemir MS, Reyngoudt H, De Deene Y, Sazak HS, Fieremans E, Delputte S, D’Asseler Y, Derave W, Lemahieu I, Achten E (2007) Absolute quantification of carnosine in human calf muscle by proton magnetic resonance spectroscopy. Phys Med Biol 52(23): 6781–6794
Keevil SF, Barbiroli B, Brooks JC, Cady EB, Canese R, Carlier P, Collins DJ, Gilligan P, Gobbi G, Hennig J, Kugel H, Leach MO, Metzler D, Mlynarik V, Moser E, Newbold MC, Payne GS, Ring P, Roberts JN, Rowland IJ, Thiel T, Tkac I, Topp S, Wittsack HJ, Wylezinska M, Zaniol P, Henriksen O, Podo F (1998) Absolute metabolite quantification by in vivo NMR spectroscopy: II. A multicentre trial of protocols for in vivo localised proton studies of human brain. Magn Reson Imaging 16(9): 1093–1106
Ethofer T, Mader I, Seeger U, Helms G, Erb M, Grodd W, Ludolph A, Klose U (2003) Comparison of longitudinal metabolite relaxation times in different regions of the human brain at 1.5 and 3 Tesla. Magn Reson Med 50(6): 1296–1301
Frahm J, Bruhn H, Gyngell ML, Merboldt KD, Hanicke W, Sauter R (1989) Localized proton NMR spectroscopy in different regions of the humanbrain in vivo. Relaxation times and concentrations of cerebral metabolites. Magn Reson Med 11(1): 47–63
Kreis R, Fusch C, Maloca P, Felbinger J, Boesch C (1994) Supposed pathology may be individuality: interindividual and regional differences of brain metabolite concentration determined by 1H MRS. In Proceedings of 2nd meeting of the society of magnetic resonance. San Francisco, USA, 45pp
Kreis R, Ernst T, Ross BD (1993) Absolute quantitation of water and metabolites in the human brain. II. Metabolite concentrations. J Magn Reson B 102(1): 9–19
Clark JB (1998) N-acetyl aspartate: a marker forn neuronal loss or mitochondrial dysfunction. Dev Neurosci 20(4–5): 271–276
Lange T, Dydak U, Roberts TP, Rowley HA, Bjeljac M, Boesiger P (2006) Pitfalls in lactate measurements at 3T. AJNR Am J Neuroradiol 27(4): 895–901
Clementi V, Tonon C, Lodi R, Malucelli E, Barbiroli B, Iotti S (2005) Assessment of glutamate and glutamine contribution to in vivo N-acetylaspartate quantification in human brain by 1H- magnetic resonance spectroscopy. Magn Reson Med 54(6): 1333–1339
Malucelli E, Manners DN, Testa C, Tonon C, Lodi R, Barbiroli B, Iotti S (2009) Pitfalls and advantages of different strategies for the absolute quantification of N-acetylaspartate, creatine and choline in white and grey matter by 1H-MRS. NMR Biomed 22(10): 1003–1013
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Reyngoudt, H., De Deene, Y., Descamps, B. et al. 1H-MRS of brain metabolites in migraine without aura: absolute quantification using the phantom replacement technique. Magn Reson Mater Phy 23, 227–241 (2010). https://doi.org/10.1007/s10334-010-0221-z
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DOI: https://doi.org/10.1007/s10334-010-0221-z