Abstract
To evaluate the ability of MR T2 mapping (8.5 T) to characterize ex vivo longitudinally, morphologically and quantitatively, alginate-based tissue engineering in a rat model of patellar cartilage chondral focal defect. Calibrated rat patellar cartilage defects (1.3 mm) were created at day 0 (D0) and alginate sponge with (Sp/C+) or without (Sp/C−) autologous chondrocytes were implanted. Animals were sacrificed sequentially at D20, D40 and D60 after surgery and dissected patellae underwent MRI exploration (8.5 T). T2 values were calculated from eight SE images by using nonlinear least-squares curve fitting on a pixel-by-pixel basis (constant repetition time of 1.5 s, eight different echo times: 5.5, 7.5, 10.5, 12.5, 15.0, 20.0, 25.0 and 30.0 ms). On the T2 map, acquired in a transversal plane through the repair zone, global T2 values and zonal variation of T2 values of repair tissue were evaluated versus control group and compared with macroscopic score and histological studies (toluidine blue, sirius red and hematoxylin-eosin). “Partial”, “total” and “hypertrophic” repair patterns were identified. At D40 and D60, Sp/C+ group was characterized by a higher proportion of “total” repair in comparison to Sp/C− group. At D60, the proportion of “hypertrophic” repair was two fold in Sp/C− group versus Sp/C+ group. As confirmed morphologically and histologically, the T2 map also permitted the distinction of three types of repair tissue: “total”, “partial” and “hypertrophic”. “Total” repair tissue was characterized by high T2 values versus normal cartilage (p<0.05). Zonal variation, reflecting the collagen network organization, appeared only at D60 for Sp/C+ group (p<0.05). “Hypertrophic” tissue, mainly observed at D60, presented high T2 global values without zonal variation with cartilage depth. These results confirm the potency of the MR T2 map (8.5 T) to characterize macroscopically and microscopically the patterns of the scaffold guided-tissue repair of a focal chondral lesion in the rat patella (“total”, “partial” and “hypertrophic”). On T2 map, three parameters (i.e. MRI macroscopic pattern, T2 global values and zonal variation of T2 values) permit to characterize chondral repair tissue, as a virtual biopsy.
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References
Hunziker EB (2002) Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects. Osteoarthritis Cartilage 10:432–463
Dougados M, Ayral X, Listrat V, et al. (1994) The SFA system for assessing articular cartilage lesions at arthroscopy of the knee. Arthroscopy 10:69–77
Alparslan L, Minas T, Winalski CS (2001) Magnetic resonance imaging of autologous chondrocyte implantation. Semin Ultrasound CT MR 22:341–351
Azer NM, Winalski CS, Minas T (2004) MR imaging for surgical planning and postoperative assessment in early osteoarthritis. Radiol Clin North Am 42:43–60
Watrin-Pinzano A, Ruaud JP, Cheli Y, et al. (2004) T2 mapping: an efficient MR quantitative technique to evaluate spontaneous cartilage repair in rat patella. Osteoarthritis Cartilage 12:191–200
Mlynarik V, Szomolanyi P, Toffanin R, Vittur F, Trattnig S (2004) Transverse relaxation mechanisms in articular cartilage. J Magn Reson.169:300–307
Watrin A, Ruaud JP, Olivier PT, et al. (2001) T2 mapping of rat patellar cartilage. Radiology 219:395–402
Loeuille D, Olivier P, Watrin A, et al. (2002) The biochemical content of articular cartilage: an original MRI approach. Biorheology 39:269–276
Dausse Y, Grossin L, Miralles G, et al. (2003) Cartilage repair using new polysaccharidic biomaterials: macroscopic, histological and biochemical approaches in a rat model of cartilage defect. Osteoarthritis Cartilage 11:16–28
McAlindon TE, Snow S, Cooper C, Dieppe PA (1992) Radiographic patterns of osteoarthritis of the knee joint in the community: the importance of the patellofemoral joint. Ann Rheum Dis 51:844–849
Hangody L, Rathonyi GK, Duska Z, Vasarhelyi G, Fules P, Modis L (2004) Autologous osteochondral mosaicplasty. Surgical technique. J Bone Joint Surg Am 86A:65–72
Brittberg M, Lindahl A, Nilsson A, Ohlsson C, Isaksson O, Peterson L (1994) Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 331:889–895
Mooney V, Ferguson AB Jr (1966) The influence of immobilization and motion on the formation of fibrocartilage in the repair granuloma after joint resection in the rabbit. J Bone Joint Surg Am 48:1145–1155
Jackson DW, Lalor PA, Aberman HM, Simon TM (2001) Spontaneous repair of full-thickness defects of articular cartilage in a goat model. A preliminary study. J Bone Joint Surg Am 83A:53–64
Breinan HA, Martin SD, Hsu HP, Spector M (2000) Healing of canine articular cartilage defects treated with microfracture, a type-II collagen matrix, or cultured autologous chondrocytes. J Orthop Res 18:781–789
Yoshioka M, Kubo T, Coutts RD, Hirasawa Y (1998) Differences in the repair process of longitudinal and transverse injuries of cartilage in the rat knee. Osteoarthritis Cartilage 6:66–75
Espanha MM, Lammi PE, Hyttinen MM, Lammi MJ, Helminen HJ (2001) Extracellular matrix composition of full-thickness defect repair tissue is little influenced by exercise in rat articular cartilage. Connect Tissue Res 42:97–109
Ibusuki S, Iwamoto Y, Matsuda T (2003) System-engineered cartilage using poly(N-isopropylacrylamide)-grafted gelatine as in situ-formable scaffold: in vivo performance. Tissue Eng 9:1133–1142
Wakitani S, Goto T, Young RG, Mansour JM, Goldberg VM, Caplan AI (1998) Repair of large full-thickness articular cartilage defects with allograft articular chondrocytes embedded in a collagen gel. Tissue Eng 4:429–444
Im GI, Kim DY, Shin JH, Hyun CW, Cho WH (2001) Repair of cartilage defect in the rabbit with cultured mesenchymal stem cells from bone marrow. J Bone Joint Surg Br 83:289–294
Miralles G, Baudoin R, Dumas D, et al. (2001) Sodium alginate sponges with or without sodium hyaluronate: in vitro engineering of cartilage. J Biomed Mater Res 57:268–278
Mierisch CM, Wilson HA, Turner MA, et al. (2003) Chondrocyte transplantation into articular cartilage defects with use of calcium alginate: the fate of the cells. J Bone Joint Surg Am 85A(9):1757–1767
Fragonas E, Valente M, Pozzi-Mucelli M, et al. (2000) Articular cartilage repair in rabbits by using suspensions of allogenic chondrocytes in alginate. Biomaterials 21:795–801
Sanders TG, Mentzer KD, Miller MD, Morrison WB, Campbell SE, Penrod BJ (2001) Autogenous osteochondral “plug” transfer for the treatment of focal chondral defects: postoperative MR appearance with clinical correlation. Skeletal Radiol 30:570–578
Goodwin DW (2001) Visualization of the macroscopic structure of hyaline cartilage with MR imaging. Semin Musculoskelet Radiol 5:305–312
Nieminen MT, Rieppo J, Toyras J, et al. (2001) T2 relaxation reveals spatial collagen architecture in articular cartilage: a comparative quantitative MRI and polarized light microscopic study. Magn Reson Med 46:487–493
Nishida T, Kubota S, Kojima S, et al. (2004) Regeneration of defects in articular cartilage in rat knee joints by CCN2 (connective tissue growth factor). J Bone Miner Res 19:1308–1319
Kawasaki K, Sugihara S, Nishida K, et al. (2004) Hoechst 33342 is a useful cell tracer for a long-term investigation of articular cartilage repair. Arch Histol Cytol. 67:13–19
Verstraete KL, Almqvist F, Verdonk P, et al. (2004) Magnetic resonance imaging of cartilage and cartilage repair. Clin Radiol 59:674–689
Roberts S, McCall IW, Darby AJ, Menage J, Evans H, Harrison PE, Richardson JB (2003). Autologous chondrocyte implantation for cartilage repair: monitoring its success by magnetic resonance imaging and histology. Arthritis Res Ther 5:R60–R73
Acknowledgments.
The authors thank Venkatesan Narayanan for his expert advice, Stéphanie Etienne for her technical assistance and Michel Thiery for his good care to animals. This work was supported by grants from the Contrat de Projet de Recherche Clinique (2000), the « Fondation pour la Recherche Médicale », the “Région Lorraine”, IT2B and ACI « CARTIGELIMAGE ».
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Watrin-Pinzano, A., Ruaud, JP., Cheli, Y. et al. Evaluation of cartilage repair tissue after biomaterial implantation in rat patella by using T2 mapping. MAGMA 17, 219–228 (2004). https://doi.org/10.1007/s10334-004-0071-7
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DOI: https://doi.org/10.1007/s10334-004-0071-7