Abstract
We examined soil N dynamics, including inorganic N concentration, net N transformation rates, and estimated plant N uptake (EPNU) from soil N budgets, and litterfall inputs, in five Japanese cedar plantation stands of different ages (5, 16, 31, 42, and 89 years) in the Mt Gomadan Experimental Forest (GEF). Net soil N mineralization and nitrification rates did not differ significantly between the youngest and oldest stands; soil moisture and inorganic N concentration were higher in the youngest stand. The EPNU was highest in the 16-year-old stand and lowest in the 31-year-old stand, and had a significant negative correlation with litter C:N ratio. The oldest (89-year-old) stand had a higher soil C:N ratio, lower proportion of nitrification rate to mineralization rate (%NIT), and higher estimated plant NH4 + uptake than did the other stands, indicating that changes of soil organic matter quality can alter soil N dynamics. These results suggest that as a Japanese cedar plantation develops, soil N dynamics can be altered by the quantity and quality of input litter and soil organic matter, and can generate the imbalance between N supply from soil and N demand by plant.
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Introduction
Internal nitrogen (N) cycling between plants and soil is very important in N retention of forested ecosystems (Vitousek and Howarth 1991; Davidson et al. 1992). One important indicator of internal N cycling is soil N availability, which often limits net primary production (NPP) in temperate forest ecosystems (Reich et al. 1997; Tateno et al. 2004). According to previous research, soil N availability is the potential of a soil to produce the inorganic N (mainly ammonium and nitrate N) available to plants. Soil N availability is regulated by N mineralization and nitrification rates, denitrification potential, and the physical absorption–desorption capacity of the soil, and atmospheric N deposition (Pastor et al. 1984; Davidson et al. 1992; Schimel and Bennett 2004; Booth et al. 2005). These factors are sensitive to tree species composition (Lovett et al. 2004), litterfall quality (Hobbie 1992), soil moisture and temperature (Stark and Firestone 1995), the quality of organic matter, for example carbon (C) to N ratio (C:N) (Lovett et al. 2004; Gundersen et al. 2006), climatic conditions (Dyer et al. 1990), and soil composition (Hall et al. 2004; Page and Mitchell 2008).
Internal N cycling is also sensitive to natural and anthropogenic disturbances (Aber et al. 2002; Galloway et al. 2004; LeBauer and Treseder 2008). Increased concern about human-related N loss in forest ecosystems has prompted research on how forest disturbance affects internal N cycling and N retention systems (Vitousek et al. 1997; Gundersen et al. 2006). Among the most serious disturbances is clear-cut harvesting, which causes large N losses via biomass removal and hydrological N leaching (Bormann and Likens 1979; Aber et al. 2002), most likely because of elimination of plant N uptake, and enhanced N mineralization and nitrification rates in soils resulting from increased soil moisture and temperature (Likens et al. 1969; Vitousek et al. 1982; Fisk and Fahey 1990; Burns and Murdoch 2005). Although the effects of forest disturbance on N cycling have been well-documented, the disturbance recovery processes during subsequent re-establishment of forest stands have not yet been clarified because long-term observations are required. Detailed knowledge of forest ecosystem recovery processes is crucial to clarifying biogeochemical functions in forest ecosystems and to developing better forest-management strategies.
Long-term observations before and after a disturbance, and chronosequential approaches, are needed to evaluate how forests develop after the disturbance. Recovery processes in forest ecosystems can be elucidated by comparing stands with similar features except “stand age” (i.e., years after disturbance and subsequent secondary growth or replanting; Johnson and Miyanishi 2008). Several studies examining N cycling in a wide range of forest ecosystems have used the chronosequence approach to document changes during forest re-establishment after clear-cutting, wildfire, or land use change (Idol et al. 2003; White et al. 2004; Bond-Lamberty et al. 2006; Yermakov and Rothstein 2006; Davidson et al. 2007). However, these studies were unable to provide a sufficient explanation of how tree growth processes affect N cycling, because they compared forest stands that differed in tree species composition, soil properties, geology, or climatic conditions.
Artificial forests account for 41% of all forest area in Japan, and Japanese cedar Cryptomeria japonica D. Don is the dominant plantation species (comprising approximately 45% of the artificial forest area in Japan). These plantation forests have undergone, and will continue to undergo, intensive disturbances, for example clear-cutting. Thus, clarifying the biogeochemical functions of forest ecosystems in Japan requires determining how clear-cutting and subsequent stand development affects internal N cycling in Japanese cedar plantations. Tateno et al. (2009) described chronological changes in biomass accumulation patterns and internal N cycling by examining biomass allocation and plant nutrient status in Japanese cedar stands of different ages in the Mt Gomadan Experimental Forest (GEF), central Japan. They found that soil N supply may limit plant growth and that N limitation may be enhanced under conditions of an imbalance between soil N supply and plant N demand 30 years after afforestation (Tateno et al. 2009). However, they did not explain the factors determining the changes in soil N dynamics.
In this study, we focused on soil N dynamics as an indicator of internal N cycling and the factors affecting these dynamics. We used the chronosequence approach to investigate changes in soil N dynamics during the development of Japanese cedar plantations. We compared soil N availability, including inorganic N concentrations, mineralization and nitrification rates, and annual N budgets, within the soil, and litterfall input, as factors affecting soil N dynamics among Japanese cedar stands of different ages. Our objectives were to clarify the patterns of soil N dynamics during stand development and to elucidate possible mechanisms altering soil N dynamics.
Materials and methods
Study site description
This study was conducted at the Mt Gomadan Experimental Forest (GEF) in Nara Prefecture, central Japan (34°04′N, 135°35′E, Fig. 1). The watersheds within GEF consist primarily of plantations of Japanese cedar (Cryptomeria japonica), and small areas of Japanese cypress (Chamaecyparis obtusa) plantations along the ridge. Because forest management is conducted on a watershed basis, stand ages are identical within each watershed but differ among watersheds. In GEF, two-year-old conifer seedlings are planted the year after clear-cutting. Hereafter, we define the stand age in 2004 as the years since clear-cutting and subsequent replanting. After forest clear-cutting, slash and logging debris are gathered and arranged into strips, and seedlings are planted. Further details of forest management in GEF can be found elsewhere (Fukushima and Tokuchi 2008, 2009).
The area overlays Cretaceous sedimentary rock composed of alternating sandstone, mudstone, and shale. Soils are classified as Cambisols or brown forest soils (Forest Soil Division 1976). Soil depth is shallow, in part because the forest slopes are very steep (average slope 30–70%; Fukushima and Tokuchi 2009). The mean annual temperature is 12.3°C, and the mean annual precipitation is 2,521 mm (averaged 1976–2005), as measured at the Wakayama Experimental Research Station, Field Science Education and Research Center, Kyoto University (34°04′N, 135°41′E, 533 m above sea level), located 4 km west of GEF (Field Science Education and Research Center 2007). Snowpack varies interannually and spatially, but is usually continuous from January to March, with depths <0.7 m on the upper slopes.
Plant censuses were conducted in five watersheds: JC5, JC16, JC31, JC42, and JC89, composed of 5, 16, 31, 42, and 89-year-old stands, respectively (Fig. 1). We established two subplots (20 × 20 m) on lower slopes with a southerly aspect and surveyed stand characteristics in 2002. Table 1 summarizes stand characteristics at each site.
Soil sampling
Soil samples were collected in the same watersheds as the plant censuses; sampling was conducted in April, July, September, and December 2003 and April 2004 (Fig. 1). We dug soil pits 50 cm deep and >1 m wide, with the exception of JC89, where soil did not extend below 30 cm because this layer contained mostly large gravel and cobbles and little fine soil. All soil pits were dug within plant census plots. Three replicates of each soil layer (0–10, 10–30, and 30–50 cm mineral soil) were collected in each watershed using a stainless steel hand trowel.
In the field, the soil samples were divided in half. One half was placed in a clean polyethylene bag in a cool box with refrigerant and brought to the laboratory at Kyoto University. The other half was also placed in a clean polyethylene bag, but it was returned to its original position for in situ incubation (‘buried bag method’, Eno 1960). The buried samples were dug up at the next soil sampling and brought to the laboratory. The incubation periods were designated season I (April–July 2003; 68–77 days); season II (July–September 2003; 92–96 days); season III (September–December 2003; 65–70 days); and season IV (December 2003–April 2004; 140–142 days). All soil samples were sieved through a 2-mm mesh to break up soil aggregates; gravel and fine roots were removed by hand and with tweezers. The treated soil samples were stored at 4°C before analysis.
In addition to soil sampling, we used an ion-exchange resin (IER) bag to determine the input and output of ionized soil solutes in each soil layer of each soil pit (Giblin et al. 1994). Each IER bag contained 7.5 g anionic resin and 7.5 g cationic resin within a polyvinyl chloride (PVC) pipe (inner diameter 5 cm, depth 1 cm) and was tightly wrapped with water-permeable nylon mesh. Four IER bags were installed at depths of 10, 30, and 50 cm in each stand (the 50 cm depth was unavailable at JC89) and retrieved at the next soil survey. The retrieved resins were opened, air-dried, and sealed before chemical analysis.
The soil bulk density of the samples was determined for each soil layer (0–10, 10–30, 30–50 cm; the 30–50 cm depth was unavailable at JC89) in each watershed in spring 2004, using 400 cm2 soil blocks.
Soil chemical analysis
Fresh soil samples (2–3 g) were oven-dried at 105°C for 48 h to determine water content. A 5-g (fresh mass) subsample of each soil sample was placed in a clean polyethylene bottle containing 50 mL 2 M KCl and shaken mechanically for 1 h to extract inorganic N. Extracts were filtered through Advantec no. 6 filter paper (Advantec MFS, Tokyo, Japan). Filtrates were frozen until colorimetric analysis for ammonium (NH4 +) and nitrate (NO3 −), which contains oxidized nitrite (NO2 −) concentrations, using a Bran + Luebee AutoAnalyzer III (BLTEC, Osaka, Japan). Soils incubated at the study sites were also extracted and analyzed as described above. The in-situ net N mineralization rate during incubation was calculated as the net change in NH4 + and NO3 − concentrations between the initial and incubated samples. In-situ net nitrification was calculated as the net change in NO3 − concentration. The remaining soil was air-dried before analysis for total C and N content, using an NC analyzer (NC-900; Shimadzu, Kyoto, Japan). Soil NH4 + and NO3 − concentrations were described as mgN kg-dry-soil−1, and net N mineralization and nitrification rates were described as mgN kg-dry-soil−1 per incubation period (seasons I–IV).
To measure absorbed N on the IER, a 5-g (air-dried mass) subsample of each resin was placed in a clean polyethylene bottle containing 100 mL 2 M KCl and shaken for 1 h. Extracts were filtered and analyzed for NH4 + and NO3 − concentrations as described above. Filtered extracts were adjusted to pH 7.0 with 1 M NaOH before chemical analysis, as the pH of most IER extracts was very low (<2.0).
Estimation of plant N uptake from soil N budgets
To calculate plant uptake of available inorganic N in soils, we used the following equation based on soil N budgets (Nadelhoffer et al. 1985; Tokuchi et al. 2002):
where i and j indicate soil depth (cm; i < j), produced N (i–j) was determined from in situ net N mineralization at the i–j soil layer, input N (i) and output N (j) were determined from N captured on IER bags at depths i and j, respectively, and ΔN pool (i–j) was the net increment in soil inorganic N content in the i–j soil layer.
Nitrogen input into the uppermost soil layer (0 cm) was estimated from atmospheric or throughfall NO3 − and NH4 + deposition data for 2005 and 2006 (Fukushima 2009). All terms in Eq. 1 are expressed as kgN ha−1 for a specific period, using soil bulk density. The calculation was performed for each soil layer and each form of N (NH4 + and NO3 −-N) during each season. Then annual plant N uptake was determined by summing all seasons throughout all soil layers. We used N mineralization rate to evaluate soil properties, and used EPNU as an indicator of internal N cycling in each stand.
Litterfall measurement
To estimate the mass of annual litterfall, ten 0.25-m2 traps with 1-mm nylon mesh were installed at a height of 1.3 m in all soil survey watersheds except JC5, where tree heights were too low (<1.3 m). Five litter traps were randomly placed within the plant census plots (20 × 20 m), with one established on each bank of the stream in each watershed. Fallen litter was collected monthly from April 2003 to April 2004. Samples were dried at 40°C for at least 2 weeks, sorted into needles, branches, other materials (cones and seeds), and litter from other species, and then weighed. The C and N content of needles was measured using an NC analyzer. We used the following chemical data as described by Igahara and Nakagawa (2002) and Inagaki et al. (2004) for branches, other materials, and other species: C content, 53.4, 53.2, and 51.9%; N content, 0.3, 1.0, and 1.5%, respectively.
Statistical analysis
Comparison of watersheds with the same climatic and geological conditions, for example those in GEF, enables straightforward analysis of how stand age affects N dynamics, and helps clarify stand development processes after clear-cutting and subsequent afforestation (Johnson and Miyanishi 2008; Tokuchi and Fukushima 2009). Our soil sampling design did not include replication of stand age, so our soil sampling and IER procedures within a watershed constitute pseudoreplication (Hurlbert 1984). This limits discussion of how stand development affects soil N dynamics. However, Japanese cedar plantation forests under the same climatic and geologic conditions have a relatively homogeneous soil environment, despite the effect of topography-mediated conditions (Hirobe et al. 1998; Tokuchi et al. 2000; Ichikawa et al. 2003). Thus, to compare soil N dynamics among forest watersheds of different ages, we established soil pits in each watershed at a similar position on a lower slope (i.e., a similar distance from a stream; Fig. 1), which enabled us to assess the effects of stand development in relation to the plant-soil-stream continuum and to compare these effects with findings from previous studies conducted in GEF (Fukushima 2009; Fukushima and Tokuchi 2009; Tateno et al. 2009; Tokuchi and Fukushima 2009) and other areas (Bohlen et al. 2001; Johnson et al. 2000).
The effects of stand age on soil moisture, inorganic N concentrations, net N mineralization rate, and net nitrification rate were analyzed using one-way analysis of variance (ANOVA), followed by Scheffe’s F test. The relationship between stand age and litterfall was analyzed using one-way ANOVA and Tukey’s post-hoc test. We also used best-fit regression and correlation analysis to examine the relationship between the soil C:N ratio and percentage nitrification and between C and N input by litterfall and EPNU. All statistical analysis was conducted by use of SPSS 10.0J (SPSS 1999).
Results
Soil moisture, inorganic N concentrations, and net N mineralization and nitrification rates
Soil moisture at 0–10 and 10–30 cm depths was significantly higher in JC5 than in the other stands at all soil depths (P < 0.05; Table 2). In deeper soil layers, soil moisture decreased in JC5 and increased in JC16 (Table 2). In JC31, JC42, and JC89, soil moisture did not differ significantly among soil layers (P > 0.05; Table 2).
The in-situ nitrification rate did not differ significantly among watersheds and soil layers, but the in-situ N mineralization rate differed significantly among soil layers in all watersheds, except in JC89, the oldest stand, and among watersheds at 30–50 cm depths (Table 2). The proportion of nitrified N to mineralized N (%NIT) exceeded 100% in almost all soil layers, indicating that NH4 + present at the start of the in-situ incubation was nitrified (Table 2). The lowest %NIT was observed at 0–10 cm depth in JC89 (70%).
Soil N budgets and estimated plant N uptake
Table 3 lists annual EPNU, input N, output N, produced N, and changes in N content. The JC5 stand received a total N deposition of 9.3 kgN ha−1 year−1. The closed-canopy JC31, JC42, and JC89 stands received total deposition of 13.9 kgN ha−1 year−1 via throughfall. For the JC16 stand, where the forest canopy was half-open, we used the arithmetic mean value between rainfall and throughfall (11.6 kgN ha−1 year−1 in total N). Output N from the bottom layer, estimated from extractable IER-captured NH4 + and NO3 −, was 23.5, 4.3, 12.0, 6.3, and 21.0 kgN ha−1 year−1, and mineralized N (i.e., produced N) in all soil layers was 30.6, 46.2, 21.3, 18.7, and 42.0 kgN ha−1 year−1 in JC5, JC16, JC31, JC42, and JC89, respectively. Based on these results, the EPNU was 18.0, 53.4, 24.8, 24.2, and 34.2 kgN ha−1 year−1 in JC5, JC16, JC31, JC42, and JC89, respectively (Table 3). Estimated plant uptake of NO3 − was highest in JC16, and that of NH4 + was highest in JC89.
Soil C:N ratio
The soil C:N ratio ranged from 10.8 to 16.6 and decreased at each stand as soil depth increased (Table 4; Tateno et al. 2009). The highest values were observed in JC89 (16.6 and 16.0 at 0–10 and 10–30 cm depths, respectively). There was a significant non-linear relationship between soil C:N ratio (CNsoil) and %NIT (Fig. 2), fitted by an exponential curve (R 2 = 0.580, P < 0.001) as follows:
Litterfall input
Annual litterfall mass was lowest in JC16 (2.5 t ha−1 year−1), highest in JC31 and JC42 (4.9 t ha−1 year−1), and intermediate in JC89 (4.2 t ha−1 year−1) (Fig. 3). Of the litter composition from all stands, needle litterfall was significantly greater in the three older stands (JC31, JC42, and JC89) than in the young stand (JC16). Branch litterfall was approximately three times greater in middle-aged stands (JC31 and JC42) than in the young stand (JC16). The amount of other materials, including cones and seeds, decreased in the order JC89 > JC42 > JC31 = JC16. Litter from other species, including broad-leaved deciduous trees and annual herbaceous plants, was significantly higher in JC16 than in the other stands.
Table 5 summarizes annual litterfall C and N amounts and the annual mean C:N ratio of litterfall. Both total C and N inputs and C:N ratio were higher in the middle-aged stands (JC31 and JC42) than in the younger (JC5) and older (JC89) stands. The relationship between EPNU and annual C input via litterfall was significantly negative (R 2 = 0.996, P < 0.001; Fig. 4a), with its r-squared being greater than that between annual litter N input and EPNU (R 2 = 0.919, P < 0.001). The correlation between EPNU and C:N ratio of litterfall was also significantly negative (R 2 = 0.958, P < 0.001; Fig. 4b).
Discussion
Effects of forest clear-cutting on soil N dynamics
Many studies have reported that when forests are clear-cut, reduced plant N uptake and enhanced microbial activity, for example that by autotrophic nitrifiers, result in high levels of NO3 − leaching and loss in soil water and streamwater (Likens et al. 1969; Vitousek et al. 1982; Fisk and Fahey 1990; Bradley 2001; Burns and Murdoch 2005). In GEF, stream NO3 − concentrations increased and peaked approximately 3 years after clear-cutting (Fukushima and Tokuchi 2008). However, we observed no significant increases in net N mineralization and nitrification rates in soil, despite the significantly higher soil moisture, especially at the 0–10 cm depth (Table 2), which is an important factor affecting soil microbial activity (Stark and Firestone 1995). Fisk and Fahey (1990) reported that enhanced net nitrification potential because of clear-cutting of northern hardwood forests in eastern North America began to decline 2 years after clear-cutting. Burns and Murdoch (2005) reported no increases in N mineralization rate or nitrification rate in clear-cut northern hardwood forests, because soil nitrification rates were high before clear-cutting. Also, the absence of increased soil net N mineralization or nitrification rates in JC5 at GEF may indicate that clear-cutting has a minimal effect on these rates, or that these effects last no longer than 5 years after clear-cutting. This JC5 watershed had significantly higher soil moisture and soil NO3 − content than other stands at the surface (0–10 cm depth) in almost all seasons, including summer, when the nitrification rate in all stands was high (Appendix A and B in the electronic supplementary material). Diminished plant activity (i.e., nutrient uptake and evapotranspiration rate) in JC5 could explain this, suggesting that cessation of plant N uptake may be the primary cause of the increased stream NO3 − concentration after clear-cutting (Fukushima and Tokuchi 2008).
Changes in soil N dynamics during forest development
Previous studies conducted in GEF have reported little change in atmospheric N input and stream NO3 − concentration in stands more than 16 years old (Fukushima and Tokuchi 2008; Fukushima 2009). In contrast, biomass accumulation rates increase after clear-cutting, peaking in stands approximately 30 years old and subsequently declining (Tateno et al. 2009).
With regard to soil N dynamics, EPNU exhibited non-linear trends as stand age increased in stands older than 16 years (Table 3). The JC16 stand exhibited the highest net N mineralization rate in all soil layers, resulting in the largest EPNU (Table 3; Appendix A in the electronic supplementary material). A possible factor determining this high mineralization rate in JC16 is the litter quality. A greater contribution from leaf litter of other species, including broad-leaved deciduous trees and annual herbaceous plants, which are likely to contain greater N concentrations than Japanese cedar needles, caused the lower C:N ratio of annual litterfall in JC16 (Table 5; Fig. 3). Many studies have reported that input litter with a high N concentration and a low C:N ratio could rapidly release inorganic N during the decomposition process and promote N mineralization in N-limited forest soil (Hobbie 1992; Chapin et al. 2002). This finding suggests that input of litter with a high N concentration could elevate the soil N status in JC16, and that the large supply of available inorganic N in the soil may support the early growth of planted Japanese cedars.
In contrast, plant N uptake estimated from the soil N budget declined remarkably in JC31 (Table 3), where Japanese cedars had the highest growth rate (i.e., productivity) of all the stands (Tateno et al. 2009). As reported by Tateno et al. (2009), an imbalance in the available N supply from soil (plant N uptake estimated from soil N budget) and plant N demand (biomass growth rate) can accelerate the N limitation status. This phase is crucial to elucidating the changes in internal N cycling during the development of Japanese cedar plantations.
The alteration of soil N dynamics in JC31 could be driven by both litter quantity and quality (Table 5). We found a negative relationship between EPNU and litterfall C (Fig. 4a) and N input. In contrast, previous studies reported a positive relationship (Pastor et al. 1984; Nadelhoffer et al. 1985; Tateno et al. 2004) or little relationship (Scott and Binkey 1997; Enoki et al. 1997) between EPNU or soil N mineralization and litterfall. They suggested that a more productive forest which produces larger amounts of litterfall would tend to be established on the soil of higher N availability. In GEF, however, we also found a significant negative correlation between EPNU and litter C:N ratio (Fig. 4b). During decomposition of organic materials with a higher C:N ratio, heterotrophic soil microbes require more N, resulting in a reduced net nitrification rate and/or a reduced proportion of nitrification to mineralization (Aber et al. 2003; Lovett et al. 2004). Moreover, at the beginning of the fresh litter decomposition process, labile C (for example water-soluble polysaccharides) leaches, as demonstrated by Nioh and Kutuna (1989), and can accelerate microbial N immobilization (Chapin et al. 2002). In addition, slowly decomposing branches comprise a significantly larger fraction of total litterfall in middle-aged stands (Fig. 3), which may enhance N immobilization during their decomposition, as reported by Vitousek et al. (1988). Therefore, although the relative importance of quality versus quantity of litterfall in its effect on soil N availability is still unknown, these results indicate that an increase in amounts of litterfall with higher C:N ratio and with a slowly decomposing fraction suppressed soil nitrification activity, and EPNU decreased in JC31.
Prescott (2002) reported that the canopy plays a key role in soil N processes as a litter producer. Because of the physical traits of Japanese cedar, the difference between its tree height and crown length (“clear-length”) increases with increasing tree height after canopy closure (Kanazawa et al. 1985; Takeshita 1985; Chiba 1990). As a result, the fall of relatively large branches with dead needles increases. In GEF, canopy closure typically takes approximately 20–30 years, during which branch litterfall increases (Fig. 3). This quantitative and qualitative change of litterfall is likely to have altered soil N dynamics and EPNU in a non-linear manner, and as a result biomass productivity declined.
The %NIT was high overall in GEF (more than 100%; Table 2); this is consistent with the findings of Hirai et al. (2006), who compiled data on soil N transformation rates in Japanese cedar forests across Japan. Of these, lower %NIT values and a higher contribution of estimated plant NH4 + uptake to total N uptake indicated that NH4 + dominated soil N dynamics in JC89. Moreover, of the inorganic N forms that serve as plant nutrients, NH4 + made a relatively higher contribution to soil inorganic N concentration in JC89 (Table 2). This is partly because the C:N ratio of soil organic matter was higher in JC89 than in the other stands (Table 4). In GEF, the %NIT was significantly and inversely correlated with mineral soil C:N ratios (Fig. 2). Similar trends between soil C:N ratios and net nitrification rate or %NIT have been reported across eastern North American (Goodale and Aber 2001; Aber et al. 2003) and European (MacDonald et al. 2002) forests, and Japanese cedar forests (Hirobe et al. 1998); this relationship can be explained as described above. Thus, an increase in soil C:N ratio with stand age after forest canopy closure is likely to result in NH4 + dominating in soil N dynamics.
White et al. (2004) reported that net N mineralization in mineral soil after clear-cutting and fire disturbance declined for the first 20 years in bigtooth aspen (Populus grandidentata) forests, and then increased to a maximum in the oldest (87-year-old) stand. These patterns were positively correlated with biomass accumulation, indicating that soil N dynamics can support continuous tree growth as stand age increases (White et al. 2004). In contrast, Tateno et al. (2009) reported that even a re-increase of net N mineralization in JC89 of GEF did not elevate the growth rate. One possible explanation of these conflicting findings may be related to the form of N preferred by plants (Haynes and Goh 1978; Nordin et al. 2001). Koba et al. (2003) investigated natural δ15N in plants and soils in a Japanese cedar plantation, and reported that Japanese cedars prefer NO3 −, particularly in high-nitrification sites on lower slopes of Mt Ryuoh, central Japan, where soil properties are similar to those in GEF. In this study, changes in the form of soil inorganic N in an old Japanese cedar stand (with elevated NH4 + availability) were shown, which may cause plant growth to remain limited by N.
Another possibility is the contribution of soil microbes to N dynamics. As soil C:N increases, N immobilization by soil microbes becomes an important pathway in soil N processes (Davidson et al. 1992; Kaye and Hart 1997; Bengtsson et al. 2003). In older GEF stands with higher soil C:N ratios, competition for inorganic N between plants and soil microbes may be more intense than in younger stands. As a result, Japanese cedars are likely to remain N limited even if EPNU increased in the old stands. These possibilities, however, cannot directly explain the reasons for the conflicts between the EPNU in soil and the growth rate of cedars in JC89. Further research is needed to elucidate the preference of the Japanese cedar for NH4 + or NO3 − as an N source and to furnish more details of soil N dynamics, presented as gross N transformation rate.
In conclusion, our findings reveal that as a Japanese cedar plantation develops, soil N dynamics are affected by both the quantity and the quality of input litter. A decline in plant N uptake, estimated from the soil N budget, is likely to be driven by plant canopy closure. These changes in soil N dynamics can generate an imbalance between N supply from soil and N demand.
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Acknowledgments
We are grateful to the staff of Wakayama Forest Research Station, FSERC, Kyoto University, for field survey support. We also thank Drs Takahito Yoshioka, Nobuhito Ohte, Keisuke Koba, and Muneto Hirobe for helpful suggestions. This study was supported by the Research Institute for Humanity and Nature (5-2 project), a Grant-in-Aid for Scientific Research (nos. 15380105 and 22780145) from the Ministry of Education, Culture, Sports, Science and Technology, a Sasakawa Grant for Science Fellows from the Japan Science Society, and the Division of Integrated Coastal Management, FSERC, Kyoto University.
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10310_2011_286_MOESM1_ESM.ppt
Supplementary Appendix A. Means of (a) soil moisture (b) NH4 + concentration, (c) NO3 − concentration, (d) net N mineralization rate, and (e) net nitrification rate in each watershed during all seasons. Bars indicate standard errors (n = 3). Left column: 0–10 cm depth; middle column: 10–30 cm depth; right column: 30–50 cm depth. Significant but complex differences appeared among both watersheds and seasons in soil moisture, NH4 + and NO3 − concentrations and net N mineralization and nitrification rates (Statistical results in Appendix B). In JC5, the youngest stand, soil moisture was higher than in other stands throughout all seasons, and NO3 − concentration was remarkably high in the summer (season II) in all layers. On the whole, at 0–10 and 10–30 cm depths, soil NH4 + and NO3 − concentrations tended to be higher in the summer (season II) and/or dormant (season IV) periods than in other seasons. At the 0–10 cm depth, two-way ANOVA revealed relatively high N mineralization and nitrification rates in summer (season II), irrespective of stand age (PPT 348 kb)
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Fukushima, K., Tateno, R. & Tokuchi, N. Soil nitrogen dynamics during stand development after clear-cutting of Japanese cedar (Cryptomeria japonica) plantations. J For Res 16, 394–404 (2011). https://doi.org/10.1007/s10310-011-0286-1
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DOI: https://doi.org/10.1007/s10310-011-0286-1