Abstract
Actinomycetes strain S-1 containing antioxidant components was isolated from the coast of Jeju Island in Korea and identified to the genus level using 16S ribosomal DNA sequencing and cellular fatty acids analysis as Nocardiopsis sp. (S-1). S-1 cultured supernatants showed high DPPH radical scavenging activities and hydroxyl radical scavenging abilities of up to 53%. Nutritional and culture conditions for antioxidant production were optimized. Galactose and yeast extracts were the best carbon and nitrogen sources for growth and antioxidant production. An initial medium acidity of pH 7.6, an incubation temperature of 25°C, a sodium chloride concentration of 2.5%, and an incubation time of 8 days were optimal. The DPPH scavenging activity of culture supernatants was 88% under optimum conditions. The S-1 strain can be useful for the functional fermented food and medicine industries.
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Ito N, Fukushima S, Tsuda H. Carcinogenicity and modification of the carcinogenic response by BHA, BHT and other antioxidants. CRC Crit. Rev. Toxicol. 15: 109–150 (1985)
Carocho M, Ferreira IC. A review on antioxidants, prooxidants and related controversy: natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food Chem. Toxicol. 51: 15–25 (2013)
Park EJ, Pezzuto JM. Antioxidant marine products in cancer chemoprevention. Antioxid. Redox. Signal. 19: 115–138 (2013)
Calabrese V, Cornelius C, Mancuso C, Lentile R, Stella AM, Butterfield DA. Redox homeostasis and cellular stress response in aging and neurodegeneration. Methods Mol. Biol. 610: 285–308 (2010)
Pandi-Perumal SR, BaHammam AS, Brown GM, Spence DW, Bharti VK, Kaur C, Hardeland R, Cardinali DP. Melatonin antioxidative defense: Therapeutical implications for aging and neurodegenerative processes. Neurotox. Res. 23: 267–300 (2013)
Lenaz G. Mitochondria and reactive oxygen species. Which role in physiology and pathology? Adv. Exp. Med. Biol. 942: 93–136 (2012)
Jena NR. DNA damage by reactive species: Mechanisms, mutation and repair. J. Biosci. 37: 503–517 (2012)
Imlay JA, Linn S. DNA damage and oxygen radical toxicity. Science 240: 1302–1309 (1988)
Kaiserová H, Simùnek T, van der Vijgh WJ, Bast A, Kvasnicková E. Flavonoids as protectors against doxorubicin cardiotoxicity: Role of iron chelation, antioxidant activity, and inhibition of carbonyl reductase. Biochim. Biophys. Acta 1772: 1065–1074 (2007)
Li Y, Xu Y, Liu L, Han Z, Lai PY, Guo X, Zhang X, Lin W, Qian PY. Five new amicoumacins isolated from a marine-derived bacterium Bacillus subtilis. Mar. Drugs. 10: 319–328 (2012)
Arumugam M, Mitra A, Jaisankar P, Dasgupta S, Sen T, Gachhui R, Kumar Mukhopadhyay U, Mukherjee J. Isolation of an unusual metabolite 2-allyloxyphenol from a marine actinobacterium, its biological activities and applications. Appl. Microbiol. Biotechnol. 86: 109–17 (2010)
Safer AM, al-Nughamish AJ. Hepatoxicity induced by the antioxidant food additive butylatedhydroxytoluene (BHT) in rats: An electron microscopial study. Histopathology 14: 391–406 (1999)
Ishikawa Y. Development of new types of antioxidants from microbial origin. J. Jpn. Oil Chem. Soc. 41: 762–767 (1992)
Karthik L, Kumar G, Bhaskara Rao KV. Antioxidant activity of newly discovered lineage of marine actinobacteria. Asian Pac. J. Trop. Med. 6: 325–332 (2013)
Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int. J. Syst. Bacteriol. 16: 313–340 (1966)
Waksman S. The Actinomyces Classification, Identification, and Description of Genera and Species. Williams and Wilkins Company, Baltimore, MD, USA. p. 327 (1961)
Prauser H. Aptness and application of colour for exact description of colours of Streptomyces. Zeitsch. Allgem. Mikrobiol. 4: 95–98 (1964)
Pridham TG, Gottlieb D. The utilization of carbon compounds by some Actinomycetalesas an aid for species determination. J. Bacteriol. 56: 107–114 (1948)
Miller L, Berger T. Bacterial Identification by Gas Chromatography of Whole Cell Fatty Acid: Hewlett-Packard Application Note. Hewlett-Packard Co., Avondale, PA, USA. pp. 228–241 (1985)
Reddy KK, Ravinder T, Kanjilal S. Synthesis and evaluation of antioxidant and antifungal activities of novel ricinoleate-based lipoconjugates of phenolic acids. Food Chem. 134: 2201–2207 (2012)
Margolin Y, Dedon PC. A general method for quantifying sequence effects on nucleobase oxidation in DNA. Methods Mol. Biol. 610: 325–340 (2010)
Fang C, Zhang J, Pang H, Li Y, Xin Y, Zhang Y. Nocardiopsis flavescens sp. nov., an actinomycete isolated from marine sediment. Int. J. Syst. Evol. Microbiol. 61: 2640–2645 (2011)
Wang X, Fang H, Huang Z, Shang W, Hou T, Cheng A, Cheng H. Imaging ROS signaling in cells and animals. J. Mol. Med. 91: 917–927 (2013)
Folbergrová J, Ješina P, Nùsková H, Houštìk J. Antioxidant enzymes in cerebral cortex of immature rats following experimentally-induced seizures: upregulation of mitochondrial MnSOD (SOD2). Int. J. Dev. Neurosci. 31: 123–130 (2013)
Ulrih NP, Adamlje U, Nemec M, Sentjurc M. Temperature- and pH-induced structural changes in the membrane of the hyperthermophilic archaeon Aeropyrum pernix K1. J. Membrane Biol. 219: 1–8 (2007)
Wang ZS, Gu YX, Yuan QS. Effect of nutrition factors on synthesis of superoxide dismutase, catalase, and membrane lipid peroxide levels in Cordyceps militaris mycelium. Curr. Microbiol. 52: 74–79 (2006)
Singh AB, Khaliq T. Chaturvedi JP, Narender T, Srivastava AK. Anti-diabetic and anti-oxidative effects of 4-hydroxypipecolic acid in C57BL/KsJ-db/db mice. Hum. Exp. Toxicol. 31: 57–65 (2012)
Raju R, Piggott AM, Quezada M, Capon RJ. Norcardiopsins C and D and nocardiopyrone A: New polypeptides from an Australian marine-derived Nocardiopsis sp. Tetrahedron 69: 692–698 (2013)
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Kim, MC., Lee, J., Kim, DH. et al. Isolation and identification of antioxidant producing marine-source actinomycetes and optimal medium conditions. Food Sci Biotechnol 23, 1629–1635 (2014). https://doi.org/10.1007/s10068-014-0222-1
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DOI: https://doi.org/10.1007/s10068-014-0222-1