Summary.
We tested whether the mechanisms of chromosome movement during anaphase in locust (Locusta migratoria L.) spermatocytes might be similar to those described for crane-fly spermatocytes. Actin and myosin have been implicated in anaphase chromosome movements in crane-fly spermatocytes, as indicated by the effects of inhibitors and by the localisations of actin and myosin in spindles. In this study, we tested whether locust spermatocyte spindles also utilise actin and myosin, and whether actin is involved in microtubule flux. Living locust spermatocytes were treated with inhibitors of actin (latrunculin B and cytochalasin D), myosin (BDM), or myosin phosphorylation (Y-27632 and ML-7). We added drugs (individually) during anaphase. Actin inhibitors alter anaphase: chromosomes either completely stop moving, slow, or sometimes accelerate. The myosin inhibitor, BDM, also alters anaphase: in most cases, the chromosomes drastically slow or stop. ML-7, an inhibitor of MLCK, causes chromosomes to stop, slow, or sometimes accelerate, similar to actin inhibitors. Y-27632, an inhibitor of Rho-kinase, drastically slows or stops anaphase chromosome movements. The effects of the drugs on anaphase movement are reversible: most of the half-bivalents resumed movement at normal speed after these drugs were washed out. Actin and myosin were present in the spindles in locations consistent with their possible involvement in force production. Microtubule flux along kinetochore fibres is an actin-dependent process, since LatB completely removes or drastically reduces the gap in microtubule acetylation at the kinetochore. These results suggest that actin and myosin are involved in anaphase chromosome movements in locust spermatocytes.
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References
GA Ault (1984) ArticleTitleUnipolar orientation stability of the sex univalent in the grasshoper (Melanoplus sanguinipes) Chromosoma 89 201–205 Occurrence Handle10.1007/BF00295000
JG Ault (1986) ArticleTitleStable versus unstable orientations of sex chromosomes in two grasshopper species Chromosoma 93 298–304 Occurrence Handle3698743 Occurrence Handle10.1007/BF00327587 Occurrence Handle1:STN:280:DyaL287ptFKmsA%3D%3D
JG Ault RB Nicklas (1989) ArticleTitleTension, microtubule rearrangements, and the proper distribution of chromosomes in mitosis Chromosoma 98 33–39 Occurrence Handle2766878 Occurrence Handle10.1007/BF00293332 Occurrence Handle1:STN:280:DyaL1Mzls1WltQ%3D%3D
K Ayscough (1998) Use of latrunculin-A, an actin monomer-binding drug R Vallee (Eds) Molecular motors and the cytoskeleton, part B. Methods in enzymology NumberInSeries298 Academic Press London 18–25 Occurrence Handle10.1016/S0076-6879(98)98004-1
AM Beier M Hauser (1981) ArticleTitleDie Chromosomenbewegung in der Anaphase Verh Dtsch Zool Ges 1981 85–96
K Belar (1929) ArticleTitleBeiträge zur Kausalanalyse der Mitose. II. Untersuchungen an den Spermatocyten von Chorthippus (Stenobothrus) lineatus Pans Z Wiss Biol Arch Entwicklungsmechanik 118 359–484
LA Cameron G Yang D Cimini JC Canman O Kisurina-Evgenieva A Khodjakov G Danuser ED Salmon (2006) ArticleTitleKinesin 5-independent poleward flux of kinetochore microtubules in PtK1 cells J Cell Biol 173 173–179 Occurrence Handle16636143 Occurrence Handle10.1083/jcb.200601075 Occurrence Handle1:CAS:528:DC%2BD28XktVCgu74%3D
W Chen D Zhang (2004) ArticleTitleKinetochore fibre dynamics outside the context of the spindle during anaphase Nat Cell Biol 6 227–231 Occurrence Handle15039774
JA Cooper (1987) ArticleTitleEffects of cytochalasin and phalloidin on actin J Cell Biol 105 1473–1478 Occurrence Handle3312229 Occurrence Handle10.1083/jcb.105.4.1473 Occurrence Handle1:CAS:528:DyaL2sXmtlWmsb8%3D
M Coue SL Brenner I Spector ED Korn (1987) ArticleTitleInhibition of actin polymerization by latrunculin A FEBS Lett 213 316–318 Occurrence Handle3556584 Occurrence Handle10.1016/0014-5793(87)81513-2 Occurrence Handle1:CAS:528:DyaL2sXitV2isbo%3D
AM Daub M Hauser (1986) ArticleTitleIn vivo effects of ortho-vanadate on spindle structure and dynamics of locust spermatocytes I Chromosoma 93 271–280 Occurrence Handle10.1007/BF00292749 Occurrence Handle1:CAS:528:DyaL28XhvVajtr0%3D
AM Daub M Hauser (1988) ArticleTitleTaxol affects meiotic spindle function in locust spermatocytes Protoplasma 142 147–155 Occurrence Handle10.1007/BF01290871
L Fabian A Forer (2005) ArticleTitleRedundant mechanisms for anaphase chromosome movements: crane-fly spermatocyte spindles normally use actin filaments but also can function without them Protoplasma 225 169–184 Occurrence Handle16228898 Occurrence Handle10.1007/s00709-005-0094-6
L Fabian X Xia DV Venkitaramani KM Johansen J Johansen DJ Andrew A Forer (2007) ArticleTitleTitin in insect spermatocyte spindle fibers associates with microtubules, actin, myosin and the matrix proteins skeletor, megator and chromator J Cell Sci 120 2190–2204 Occurrence Handle17591688 Occurrence Handle10.1242/jcs.03465 Occurrence Handle1:CAS:528:DC%2BD2sXosVOiuro%3D
A Forer (1969) Chromosome movements during cell division A Lima-de-Faria (Eds) Handbook of molecular cytology North-Holland Amsterdam 553–601
A Forer (1974) Possible roles of microtubules and actin-like filaments during cell-division GM Padilla IL Cameron A Zimmerman (Eds) Cell cycle controls Academic Press New York 319–336
A Forer L Fabian (2005) ArticleTitleDoes 2,3-butanedione monoxime inhibit non-muscle myosin? Protoplasma 225 1–4 Occurrence Handle15868207 Occurrence Handle10.1007/s00709-004-0077-z Occurrence Handle1:CAS:528:DC%2BD2MXjvVSqtb0%3D
A Forer JD Pickett-Heaps (1998) ArticleTitleCytochalasin D and latrunculin affect chromosome behaviour during meiosis in crane-fly spermatocytes Chromosome Res 6 533–549 Occurrence Handle9886773 Occurrence Handle10.1023/A:1009224322399 Occurrence Handle1:CAS:528:DyaK1MXisVygsA%3D%3D
A Forer J Pickett-Heaps (2005) ArticleTitleFibrin clots keep non-adhering living cells in place on glass for perfusion or fixation Cell Biol Int 29 721–730 Occurrence Handle16095930 Occurrence Handle10.1016/j.cellbi.2005.04.010 Occurrence Handle1:CAS:528:DC%2BD2MXpslymt7o%3D
A Forer PJ Wilson (2000) ArticleTitleEvidence that kinetochore fibre microtubules shorten predominantly at the pole in anaphase flea-beetle spermatocytes Chromosome Res 8 151 Occurrence Handle10780704 Occurrence Handle10.1023/A:1009298620707 Occurrence Handle1:CAS:528:DC%2BD3cXislSnt7k%3D
A Forer T Spurck JD Pickett-Heaps PJ Wilson (2003) ArticleTitleStructure of kinetochore fibres in crane-fly spermatocytes after irradiation with an ultraviolet microbeam: neither microtubules nor actin filaments remain in the irradiated region Cell Motil Cytoskeleton 56 173–192 Occurrence Handle14569597 Occurrence Handle10.1002/cm.10144
N Gawadi (1971) ArticleTitleActin in the mitotic spindle Nature 234 410 Occurrence Handle4945905 Occurrence Handle10.1038/234410a0 Occurrence Handle1:CAS:528:DyaE38Xps1Sktg%3D%3D
N Gawadi (1974) ArticleTitleCharacterization and distribution of microfilaments in dividing locust testis cells Cytobios 10 17–35
AB Ilagan A Forer (1997) ArticleTitleEffects of ultraviolet-microbeam irradiation of kinetochores in crane-fly spermatocytes Cell Motil Cytoskeleton 36 266–275 Occurrence Handle9067622 Occurrence Handle10.1002/(SICI)1097-0169(1997)36:3<266::AID-CM7>3.0.CO;2-5 Occurrence Handle1:STN:280:DyaK2s3itVClsg%3D%3D
T Ishizaki M Uehata I Tamechika J Keel K Nonomura M Maekawa S Narumiya (2000) ArticleTitlePharmacological properties of Y-27632, a specific inhibitor of Rho-associated kinases Mol Pharmacol 57 976–983 Occurrence Handle10779382 Occurrence Handle1:CAS:528:DC%2BD3cXjtVegu74%3D
B John GM Hewitt (1966) ArticleTitleKaryotype stability and DNA variability in the Acrididae Chromosoma 20 155–172 Occurrence Handle10.1007/BF00335205
K Katoh Y Kano M Amano K Kaibuchi K Fujiwara (2001) ArticleTitleStress fiber organization regulated by MLCK and Rho-kinase in cultured human fibroblasts Am J Physiol Cell Physiol 280 C1669–C1679 Occurrence Handle11350763 Occurrence Handle1:CAS:528:DC%2BD3MXksFKms74%3D
JV Kilmartin B Wright C Milstein (1982) ArticleTitleRat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line J Cell Biol 93 576–582 Occurrence Handle6811596 Occurrence Handle10.1083/jcb.93.3.576 Occurrence Handle1:STN:280:DyaL3s%2FgslChtg%3D%3D
JR LaFountain SuffixJr MA Janicke R Balczon GK Rickards (1992) ArticleTitleCytochalasin induces abnormal anaphase in crane-fly spermatocytes and causes altered distribution of actin and centromeric antigens Chromosoma 101 425–441 Occurrence Handle10.1007/BF00582837 Occurrence Handle1:CAS:528:DyaK38XltVGqtLs%3D
JR LaFountain SuffixJr CS Cohan AJ Siegel DJ LaFountain (2004) ArticleTitleDirect visualization of microtubule flux during metaphase and anaphase in crane-fly spermatocytes Mol Biol Cell 15 5724–5732 Occurrence Handle15469981 Occurrence Handle10.1091/mbc.E04-08-0750 Occurrence Handle1:CAS:528:DC%2BD2cXhtVGlsLfO
C Machado CE Sunkel DJ Andrew (1998) ArticleTitleHuman antibodies reveal titin as a chromosomal protein J Cell Biol 141 321–333 Occurrence Handle9548712 Occurrence Handle10.1083/jcb.141.2.321 Occurrence Handle1:CAS:528:DyaK1cXis1GltLg%3D
P Maddox A Straight P Coughlin TJ Mitchison ED Salmon (2003) ArticleTitleDirect observation of microtubule dynamics at kinetochores in Xenopus extract spindles: implications for spindle mechanics J Cell Biol 162 377–382 Occurrence Handle12900391 Occurrence Handle10.1083/jcb.200301088 Occurrence Handle1:CAS:528:DC%2BD3sXmt1Cruro%3D
W Margolin (2005) ArticleTitleBacterial mitosis: actin in a new role at the origin Curr Biol 15 R259–R261 Occurrence Handle15823528 Occurrence Handle10.1016/j.cub.2005.03.026 Occurrence Handle1:CAS:528:DC%2BD2MXjtV2isb4%3D
F Matsumura (2005) ArticleTitleRegulation of myosin II during cytokinesis in higher eukaryotes Trends Cell Biol 15 371–377 Occurrence Handle15935670 Occurrence Handle10.1016/j.tcb.2005.05.004 Occurrence Handle1:CAS:528:DC%2BD2MXlvFygt7s%3D
CE McClung (1902) ArticleTitleThe spermatocyte divisions of the Locustidae Kans Univ Sci Bull 1 185–231
JR McIntosh EL Grishchuk RR West (2002) ArticleTitleChromosome-microtubule interactions during mitosis Annu Rev Cell Dev Biol 18 193–219 Occurrence Handle12142285 Occurrence Handle10.1146/annurev.cellbio.18.032002.132412 Occurrence Handle1:CAS:528:DC%2BD38XptVOqsr8%3D
TJ Mitchison (1989) ArticleTitlePolewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence J Cell Biol 109 637–652 Occurrence Handle2760109 Occurrence Handle10.1083/jcb.109.2.637 Occurrence Handle1:CAS:528:DyaL1MXltlSjtbk%3D
PB Moens (1969) ArticleTitleThe fine structure of meiotic chromosome polarization and pairing in Locusta migratoria spermatocytes Chromosoma 28 1–25 Occurrence Handle5365542 Occurrence Handle10.1007/BF00325986 Occurrence Handle1:STN:280:DyaE3c%2Fos1amsg%3D%3D
OL Mohr (1914) ArticleTitleStudien über die Chromatinreifung der männlichen Geschlechtszellen bei Locusta viridissima Arch Biol 29 579–752
WM Morton KR Ayscough PJ McLaughlin (2000) ArticleTitleLatrunculin alters the actin-monomer subunit interface to prevent polymerization Nat Cell Biol 2 376–378 Occurrence Handle10854330 Occurrence Handle10.1038/35014075 Occurrence Handle1:CAS:528:DC%2BD3cXlt1SlsLw%3D
S Narumiya T Ishizaki M Uehata (2000) Use and properties of ROCK-specific inhibitor Y-27632 W Balch C Der A Hall (Eds) Regulators and effectors of small GTPases, part D: Rho family SeriesTitleMethods in enzymology NumberInSeries325 Academic Press London 273–284 Occurrence Handle10.1016/S0076-6879(00)25449-9
RB Nicklas JC Waters ED Salmon SC Ward (2001) ArticleTitleCheckpoint signals in grasshopper meiosis are sensitive to microtubule attachment, but tension is still essential J Cell Sci 114 4173–4183 Occurrence Handle11739650 Occurrence Handle1:CAS:528:DC%2BD3MXptlKrtL4%3D
V Niggli M Schmid A Nievergelt (2006) ArticleTitleDifferential roles of Rho-kinase and myosin light chain kinase in regulating shape, adhesion, and migration of HT1080 fibrosarcoma cells Biochem Biophys Res Commun 343 602–608 Occurrence Handle16554034 Occurrence Handle10.1016/j.bbrc.2006.03.022 Occurrence Handle1:CAS:528:DC%2BD28XivV2rsr8%3D
A Palazzo B Ackerman GG Gundersen (2003) ArticleTitleCell biology: tubulin acetylation and cell motility Nature 421 230 Occurrence Handle12529632 Occurrence Handle10.1038/421230a Occurrence Handle1:CAS:528:DC%2BD3sXjsF2iug%3D%3D
LV Paliulis RB Nicklas (2000) ArticleTitleThe reduction of chromosome number in meiosis is determined by properties built into the chromosomes J Cell Biol 150 1223–1232 Occurrence Handle10995430 Occurrence Handle10.1083/jcb.150.6.1223 Occurrence Handle1:CAS:528:DC%2BD3cXmslGmtbg%3D
H Qi U Rath D Wang YZ Xu Y Ding W Zhang MJ Blacketer MR Paddy J Girton J Johansen KM Johansen (2004) ArticleTitleMegator, an essential coiled-coil protein that localizes to the putative spindle matrix during mitosis in Drosophila Mol Biol Cell 15 4854–4865 Occurrence Handle15356261 Occurrence Handle10.1091/mbc.E04-07-0579 Occurrence Handle1:CAS:528:DC%2BD2cXpslKgu7g%3D
U Rath D Wang Y Ding YZ Xu H Qi MJ Blacketer J Girton J Johansen KM Johansen (2004) ArticleTitleChromator, a novel and essential chromodomain protein interacts directly with the putative spindle matrix protein skeletor J Cell Biochem 93 1033–1047 Occurrence Handle15389869 Occurrence Handle10.1002/jcb.20243 Occurrence Handle1:CAS:528:DC%2BD2cXhtVaqur3J
E Rebollo P Arana (1995) ArticleTitleA comparative study of orientation at behavior of univalent in living grasshopper spermatocytes Chromosoma 104 56–67 Occurrence Handle7587595 Occurrence Handle1:STN:280:DyaK28%2FhvFSmsQ%3D%3D
H Rees A Jamieson (1954) ArticleTitleA supernumerary chromosome in Locusta Nature 173 43–44 Occurrence Handle13119743 Occurrence Handle10.1038/173043a0 Occurrence Handle1:STN:280:DyaG2c%2FktVOjtw%3D%3D
H Ris (1949) ArticleTitleThe anaphase movement of chromosomes in the spermatocytes of the grasshopper Biol Bull 96 90–106 Occurrence Handle10.2307/1538001 Occurrence Handle18108895 Occurrence Handle1:STN:280:DyaH1M%2FhtVCqsw%3D%3D
GC Rogers SL Rogers DJ Sharp (2005) ArticleTitleSpindle microtubules in flux J Cell Sci 118 1105–1116 Occurrence Handle15764594 Occurrence Handle10.1242/jcs.02284 Occurrence Handle1:CAS:528:DC%2BD2MXjs1Chs7k%3D
M Saitoh T Ishikawa S Matsushima M Naka H Hidaka (1987) ArticleTitleSelective inhibition of catalytic activity of smooth muscle myosin light chain kinase J Biol Chem 262 7796–7801 Occurrence Handle3108259 Occurrence Handle1:CAS:528:DyaL2sXksFCksLg%3D
P Sampath TD Pollard (1991) ArticleTitleEffects of cytochalasin, phalloidin, and pH on the elongation of actin filaments Biochemistry 30 1973–1980 Occurrence Handle1899622 Occurrence Handle10.1021/bi00221a034 Occurrence Handle1:CAS:528:DyaK3MXnslalsQ%3D%3D
K Sampson JD Pickett-Heaps A Forer (1996) ArticleTitleCytochalasin D blocks chromosomal attachment to the spindle in the green alga Oedogonium Protoplasma 192 130–144 Occurrence Handle10.1007/BF01273885 Occurrence Handle1:CAS:528:DyaK28XmsFSrsr4%3D
JM Sanger B Mittal JS Dome JW Sanger (1989) ArticleTitleAnalysis of cell division using fluorescently labelled actin and myosin in living PtK2 cells Cell Motil Cytoskeleton 14 201–219 Occurrence Handle2692841 Occurrence Handle10.1002/cm.970140207 Occurrence Handle1:STN:280:DyaK3c7isVKksw%3D%3D
KE Sawin TJ Mitchison (1991) ArticleTitlePoleward microtubule flux in mitotic spindles assembled in vitro J Cell Biol 112 941–954 Occurrence Handle1999464 Occurrence Handle10.1083/jcb.112.5.941 Occurrence Handle1:STN:280:DyaK3M7lsVehtg%3D%3D
AC Schmit AM Lambert (1988) ArticleTitlePlant actin filament and microtubule interactions during anaphase-telophase transitions: effect of antagonist drugs Biol Cell 64 309–319 Occurrence Handle3228622 Occurrence Handle10.1016/0248-4900(88)90005-6 Occurrence Handle1:CAS:528:DyaL1MXkt1Sqs7k%3D
AC Schmit AM Lambert (1990) ArticleTitleMicroinjected fluorescent phalloidin in vivo reveals the F-actin dynamics and assembly in higher plant mitotic cells Plant Cell 2 129–138 Occurrence Handle2136631 Occurrence Handle10.1105/tpc.2.2.129 Occurrence Handle1:CAS:528:DyaK3cXhslGksbs%3D
PJ Sillers A Forer (1981) ArticleTitleAutosomal spindle fibres influence subsequent sex-chromosome movements in crane-fly spermatocytes J Cell Sci 49 51–67 Occurrence Handle7198129 Occurrence Handle1:STN:280:DyaL38%2Fns1Snuw%3D%3D
RV Silverman-Gavrila A Forer (2000a) ArticleTitleChromosome attachment to the spindle in crane-fly spermatocytes requires actin and is necessary to initiate the anaphase-onset checkpoint Protoplasma 212 56–71 Occurrence Handle10.1007/BF01279347
RV Silverman-Gavrila A Forer (2000b) ArticleTitleEvidence that actin and myosin are involved in the poleward flux of tubulin in metaphase kinetochore microtubules of crane-fly spermatocytes J Cell Sci 113 597–609 Occurrence Handle1:CAS:528:DC%2BD3cXhvFSgtr8%3D
RV Silverman-Gavrila A Forer (2001) ArticleTitleEffects of anti-myosin drugs on anaphase chromosome movement and cytokinesis in crane-fly primary spermatocytes Cell Motil Cytoskeleton 50 180–197 Occurrence Handle11807939 Occurrence Handle10.1002/cm.10006 Occurrence Handle1:CAS:528:DC%2BD38XlvVCgsQ%3D%3D
RV Silverman-Gavrila A Forer (2003) ArticleTitleMyosin localisation during meiosis I of crane-fly spermatocytes gives indications about its role in division Cell Motil Cytoskeleton 55 97–113 Occurrence Handle12740871 Occurrence Handle10.1002/cm.10112 Occurrence Handle1:CAS:528:DC%2BD3sXltVyjtbk%3D
I Spector NR Shochet Y Kashman A Groweiss (1983) ArticleTitleLatrunculins: novel marine toxins that disrupt microfilament organization in cultured cells Science 219 493–495 Occurrence Handle6681676 Occurrence Handle10.1126/science.6681676 Occurrence Handle1:CAS:528:DyaL3sXhslCntr0%3D
CJ Staiger M Schliwa (1987) ArticleTitleActin localisation and function in higher plants Protoplasma 141 1–12 Occurrence Handle10.1007/BF01276783 Occurrence Handle1:CAS:528:DyaL1cXht1ynsrc%3D
G Totsukawa Y Yamakita S Yamashiro DJ Hartshorne Y Sasaki F Matsumura (2000) ArticleTitleDistinct roles of ROCK (Rho-kinase) and MLCK in spatial regulation of MLC phosphorylation for assembly of stress fibers and focal adhesions in 3T3 fibroblasts J Cell Biol 150 797–806 Occurrence Handle10953004 Occurrence Handle10.1083/jcb.150.4.797 Occurrence Handle1:CAS:528:DC%2BD3cXmtVemu7k%3D
G Totsukawa Y Wu Y Sasaki DJ Hartshorne Y Yamakita S Yamashiro F Matsumura (2004) ArticleTitleDistinct roles of MLCK and ROCK in the regulation of membrane protrusions and focal adhesion dynamics during cell migration of fibroblasts J Cell Biol 164 427–439 Occurrence Handle14757754 Occurrence Handle10.1083/jcb.200306172 Occurrence Handle1:CAS:528:DC%2BD2cXhtVahsrs%3D
DL Walker D Wang Y Jin U Rath Y Wang J Johansen KM Johansen (2000) ArticleTitleSkeletor, a novel chromosomal protein that redistributes during mitosis, provides evidence for the formation of a spindle matrix J Cell Biol 151 1401–1412 Occurrence Handle11134070 Occurrence Handle10.1083/jcb.151.7.1401 Occurrence Handle1:CAS:528:DC%2BD3MXoslaq
MJD White (1935) ArticleTitleThe effects of X-rays on mitosis in the spermatogonial divisions of Locusta migratoria L Proc R Soc Lond Biol Sci 119 61–84 Occurrence Handle10.1098/rspb.1935.0076
PJ Wilson A Forer (1989) ArticleTitleAcetylated alpha-tubulin in spermatogenic cells of the crane fly Nephrotoma suturalis: kinetochore microtubules are selectively acetylated Cell Motil Cytoskeleton 14 237–250 Occurrence Handle10.1002/cm.970140210 Occurrence Handle1:CAS:528:DyaK3cXhvFWhtQ%3D%3D
PJ Wilson A Forer (1997) ArticleTitleEffects of nanomolar taxol on crane-fly spermatocyte spindles indicate that acetylation of kinetochore microtubules can be used as a marker of poleward tubulin flux Cell Motil Cytoskeleton 37 20–32 Occurrence Handle9142436 Occurrence Handle10.1002/(SICI)1097-0169(1997)37:1<20::AID-CM3>3.0.CO;2-L Occurrence Handle1:CAS:528:DyaK2sXjtleqtLw%3D
PJ Wilson A Forer C Leggiadro (1994) ArticleTitleEvidence that kinetochore microtubules in crane-fly spermatocytes disassemble during anaphase primarily at the poleward end J Cell Sci 107 3015–3027 Occurrence Handle7699001 Occurrence Handle1:CAS:528:DyaK2MXisFSksbc%3D
R Wong A Forer (2003) ArticleTitle‘Signalling’ between chromosomes in crane-fly spermatocytes studied using ultraviolet microbeam irradiation Chromosome Res 11 771–786 Occurrence Handle14712863 Occurrence Handle10.1023/B:CHRO.0000005753.97458.20 Occurrence Handle1:CAS:528:DC%2BD3sXptlGltL8%3D
WA Wrench JA Snyder (2001) ArticleTitleThe effect of cytochalasin J on kinetochore structure in PtK1 cells is mitotic cycle dependent Cell Biol Int 25 815–820 Occurrence Handle11482906 Occurrence Handle10.1006/cbir.2001.0758 Occurrence Handle1:CAS:528:DC%2BD3MXlsFynsLk%3D
EG Yarmola T Somasundaram TA Boring I Spector MR Bubb (2000) ArticleTitleActin-latrunculin A structure and function. Differential modulation of actin-binding protein function by latrunculin A J Biol Chem 275 28120–28127 Occurrence Handle10859320 Occurrence Handle1:CAS:528:DC%2BD3cXmsVKktr0%3D
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Correspondence: A. Forer, Biology Department, York University, 4700 Keele Street, Toronto, ON M3J 1P3, Canada.
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Fabian, L., Forer, A. Possible roles of actin and myosin during anaphase chromosome movements in locust spermatocytes. Protoplasma 231, 201–213 (2007). https://doi.org/10.1007/s00709-007-0262-y
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DOI: https://doi.org/10.1007/s00709-007-0262-y