Abstract.
A fast method of determining ascorbic acid and isoascorbic acid by capillary zone electrophoresis with a photodiode array detector was developed. Response surface methodologies based on three-level, three-variable designs, such as the Box-Behnken design, central composite face-centered and full fractional design, were used comparatively for optimization of buffer pH, buffer concentration and operation voltage. Statistical interpretation of the variables concerning different responses, such as resolution and migration time of the last migrated analyte, were performed. The optimum conditions of these variables were predicted using a second-order polynomial model fitted to the results obtained by applying three designs. The response surface plots using three experimental designs revealed a separation optimum with Tris–HCl buffer of pH 8.5, a concentration of 50 mM, and an operation voltage of 30 kV. The significance of the statistical designs were confirmed by the generally good agreement obtained between predicted responses and actual experimental data. We concluded that experimental designs offer a rapid means of optimizing several variables and provide an efficient test for the robustness of the analytical method.
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A M Siouffi R Phan-Tan-Luu (2000) J Chromatograph A 892 75 Occurrence Handle10.1016/S0021-9673(00)00247-8 Occurrence Handle1:CAS:528:DC%2BD3cXntVSit74%3D
J Gabrielsson N O Lindberg T Lundstedt (2002) J Chemometrics 16 141 Occurrence Handle10.1002/cem.697 Occurrence Handle1:CAS:528:DC%2BD38XitFCksr8%3D
Y P Zhang K P Lee S H Kim A Gopalan Z B Yuan (2004) Electrophoresis 25 2711 Occurrence Handle10.1002/elps.200405969 Occurrence Handle1:CAS:528:DC%2BD2cXns1Sgsrk%3D
C V Suresh Bahu B C Chung Y S Yoo (2004) Anal Lett 37 2485 Occurrence Handle10.1081/AL-200029380
M Jimidar P F Aguiar S Pintelon D L Massart (1997) J Pharm Biomed Anal 15 709 Occurrence Handle10.1016/S0731-7085(96)01910-3 Occurrence Handle1:CAS:528:DyaK2sXjtFCgurw%3D
S Sentellas J Saurina (2003) J Sep Sci 26 875 Occurrence Handle10.1002/jssc.200301514 Occurrence Handle1:CAS:528:DC%2BD3sXlvV2qtbo%3D
S Sentellas J Saurina (2003) J Sep Sci 26 1395 Occurrence Handle10.1002/jssc.200301515 Occurrence Handle1:CAS:528:DC%2BD3sXovVahsrs%3D
J Gabrielsson N O Lindberg T Lundstedt (2002) J Chemometrics 16 141 Occurrence Handle10.1002/cem.697 Occurrence Handle1:CAS:528:DC%2BD38XitFCksr8%3D
Y X Zhang H Li J Havel (2005) Talanta 65 853 Occurrence Handle10.1016/j.talanta.2004.08.016 Occurrence Handle1:CAS:528:DC%2BD2MXoslCmtw%3D%3D
S Rudaz S Cherkaoui J Y Gauvrit P Lanteri J L Veuthey (2001) Electrophoresis 22 3316 Occurrence Handle10.1002/1522-2683(200109)22:15<3316::AID-ELPS3316>3.0.CO;2-R Occurrence Handle1:CAS:528:DC%2BD3MXovVyhtrc%3D
R Ficarra P Cutroneo Z Aturki S Tommasini P Ficarra (2002) J Pharm Biomed Anal 29 987
S Hillaert Y V Heyden W V Bossche (2002) J Chromatogra A 978 231 Occurrence Handle10.1016/S0021-9673(02)01388-2 Occurrence Handle1:CAS:528:DC%2BD38XotF2jsL4%3D
C Smadja I Le Potier P Chaminade C Jacquot M Taverna (2003) Chromatographia 58 79 Occurrence Handle1:CAS:528:DC%2BD3sXmsFWgur8%3D
A C Servais M Fillet P Chiap A M Abushoffa P Hubert J Crommen (2002) J Sep Sci 25 1087 Occurrence Handle10.1002/1615-9314(20021101)25:15/17<1087::AID-JSSC1087>3.0.CO;2-A Occurrence Handle1:CAS:528:DC%2BD3sXhtFKlsA%3D%3D
L M Avois P Mangin M Saugy (2003) J Chromatogr B 791 203 Occurrence Handle10.1016/S1570-0232(03)00222-8
Y L Loukas S Sabbah G K E Scriba (2001) J Chromatogra A 931 141 Occurrence Handle10.1016/S0021-9673(01)01179-7 Occurrence Handle1:CAS:528:DC%2BD3MXnsVSgtLk%3D
R Ragonese M Macka J Hughes P Petocz (2002) J Pharm Biomed Anal 27 995 Occurrence Handle10.1016/S0731-7085(01)00659-8 Occurrence Handle1:CAS:528:DC%2BD38XhtVKkt74%3D
M E P Hows D Perrett J Kay (1997) J Chromatograph A 768 97 Occurrence Handle10.1016/S0021-9673(97)00107-6 Occurrence Handle1:CAS:528:DyaK2sXisVelsrg%3D
T Liao S B Wu W C Wu H M Chang (2000) J Agric Food Chem 48 37 Occurrence Handle10.1021/jf990399e Occurrence Handle1:CAS:528:DyaK1MXnvVOgsLY%3D
Y J Tang M J Wu (2005) Talanta 65 794 Occurrence Handle10.1016/j.talanta.2004.08.013 Occurrence Handle1:CAS:528:DC%2BD2cXhtFams77I
J M Herrero-Martinez E Simo-Alfonso V I Deltoro A Calatayud G Ramis-Ramos (1998) Anal Biochem 265 275 Occurrence Handle10.1006/abio.1998.2861 Occurrence Handle1:CAS:528:DyaK1MXitVyntA%3D%3D
A Versari A Mattioli G P Parpinello S Galassi (2004) Food Control 15 355 Occurrence Handle10.1016/S0956-7135(03)00097-5 Occurrence Handle1:CAS:528:DC%2BD2cXivVCqtLg%3D
K Fukushi S Takeda S I Wakida M Yamane K Higashi K Hiiro (1997) J Chromatogr A 772 313 Occurrence Handle10.1016/S0021-9673(97)00047-2 Occurrence Handle1:CAS:528:DyaK2sXksVygsrw%3D
O K Choi J S Jo (1997) J Chromatogr A 781 435 Occurrence Handle10.1016/S0021-9673(97)00531-1 Occurrence Handle1:CAS:528:DyaK2sXlvVKlt7c%3D
M W Davey G Bauw M V Montagu (1996) Anal Biochem 239 8 Occurrence Handle10.1006/abio.1996.0284 Occurrence Handle1:CAS:528:DyaK28XksFCmt74%3D
V Harang M Tysk D Westerlund D Isaksson G Johansson (2002) Electrophoresis 23 2306 Occurrence Handle10.1002/1522-2683(200207)23:14<2306::AID-ELPS2306>3.0.CO;2-U Occurrence Handle1:CAS:528:DC%2BD38XmtFaqtr4%3D
D Ivanovic M Medenica B Jancic A Maenovic S Markovic (2004) Chromatographia 60 87 Occurrence Handle10.1365/s10337-004-0324-7
M I Jimidar T Vennekens W V Ael D Redlich M D Smet (2004) Electrophoresis 25 2876 Occurrence Handle10.1002/elps.200406031 Occurrence Handle1:CAS:528:DC%2BD2cXns1SgsLg%3D
U Alesolo L Gonzalez R M Jimenez R M Alonso (2003) J Chromatogr A 990 271 Occurrence Handle10.1016/S0021-9673(03)00055-4
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Gong, W., Zhang, Y., Choi, S. et al. Application of response surface methodologies in capillary electrophoresis. Microchim Acta 156, 327–335 (2006). https://doi.org/10.1007/s00604-006-0627-5
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DOI: https://doi.org/10.1007/s00604-006-0627-5