Abstract
Background
Hyperpolarization-activated cyclic nucleotide (HCN) channels are pacemaker channels that regulate heart rate and neuronal rhythm in spontaneously active cardiac and neuronal cells. Interstitial cells of Cajal (ICCs) are also spontaneously active pacemaker cells in the gastrointestinal tract. Here, we investigated the existence of HCN channel and its role on pacemaker activity in colonic ICCs.
Methods
We performed whole-cell patch clamp, RT-PCR, and Ca2+-imaging in cultured ICCs from mouse mid colon.
Results
SQ-22536 and dideoxyadenosine (adenylate cyclase inhibitors) decreased the frequency of pacemaker potentials, whereas both rolipram (cAMP-specific phosphodiesterase inhibitor) and cell-permeable 8-bromo-cAMP increased the frequency of pacemaker potentials. CsCl, ZD7288, zatebradine, clonidine (HCN channel blockers), and genistein (a tyrosine kinase inhibitor) suppressed the pacemaker activity. RT-PCR revealed expression of HCN1 and HCN3 channels in c-kit and Ano1 positive colonic ICCs. In recordings of spontaneous intracellular Ca2+ [Ca2+]i oscillations, rolipram and 8-bromo-cAMP increased [Ca2+]i oscillations, whereas SQ-22536, CsCl, ZD7288, and genistein decreased [Ca2+]i oscillations.
Conclusions
HCN channels in colonic ICCs are tonically activated by basal cAMP production and participate in regulation of pacemaking activity.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Interstitial cells of Cajal (ICCs) are pacemaker cells and play an important role in the regulation of smooth muscle activity by producing and propagating slow waves, transducing neural input to smooth muscles, and acting as stretch receptors in the gastrointestinal (GI) tract [1–3]. Many neurotransmitters and hormones affect the frequency and configuration of slow waves, thus regulating GI motility [4]. ICCs generate spontaneous pacemaker potentials that correlate with peristaltic contractions in the small intestine [5]. The pacemaking mechanism has been well studied in cultured ICCs and intact mouse small intestine [6, 7]. The coupling between IP3-gated Ca2+ release from endoplasmic reticulum and reuptake of Ca2+ into mitochondria is linked to generation of pacemaker activity by periodically activating Ca2+-dependent membrane pacemaker ion channels [8]. Transient receptor potential (TRP) channels or Ca2+-activated Cl− channels were candidates as pacemaker channels in ICCs [9–12]. Recently it was found that Ano1, which encodes Ca2+-activated Cl− channels and was known as a selective marker of ICCs, plays an important role in pacemaking due to loss of slow waves by the knockout of Ano1 [13, 14]. However, Ano1 is also expressed in non-active secretory epithelial cells [15–17] of the lung, kidney and salivary glands. It was also reported that Ano1 acts as a regulator for proliferation of ICCs [18].
The frequency and configuration of GI pacemaker activity is affected by temperature and energy metabolism [19, 20]. Moreover, slow waves were found to be sensitive to intracellular cAMP level in the canine colon [21, 22]. Therefore, it was suggested that an intracellular metabolic process involving Ca2+ release from endoplasmic reticulum that is sensitive to cAMP levels triggers the colonic slow waves. cAMP is a second messenger and acts as an inhibitory agent in regulating GI motility [23]. However, in spontaneously active cardiac and neuronal cells, cAMP mediates excitatory responses that increase heart rates and neuronal firing rates. Hyperpolarization-activated cyclic nucleotide (HCN) channels are pacemaker channels that present in a variety of spontaneously active cells such as heart and neurons and play an important role in regulation of cell excitability [24, 25]. These channels are activated directly by cAMP [26]. ICCs are spontaneously active pacemaker cells and are rich in mitochondria, suggesting that HCN channels may exist in these cells. Thus, in this present study, we performed a series of functional and molecular experiments to investigate whether HCN channels exist in colonic ICCs and what physiological roles they have in the pacemaking activity of the mouse mid-colon.
Methods
Preparation of cells and tissues
The protocols and animal care used in these experiments were in accordance with the guiding principles approved by the ethics committee in Chosun University and the National Institutes of Health Guide, South Korea for the Care and Use of Laboratory Animals. Mice had free access to water and a standard mouse diet until the day of experimentation. Balb/C mice (5–8 days old) of either sex were anesthetized with ether and sacrificed by cervical dislocation. The small intestines from 1 cm below the pyloric ring to the cecum were removed and opened along the mesenteric border. The colon from below cecum to rectum were removed and mid region of the colon was used. Colon were opened along the mesenteric border. The luminal contents were washed away with Krebs–Ringer bicarbonate solution. The tissues were pinned to the base of a Sylgard dish and the mucosa was removed by sharp dissection. Small strips of intestinal muscle or colonic muscle were equilibrated in Ca2+-free Hank’s solution for 30 min, and the cells were dispersed with an enzyme solution containing 1.3 mg/ml collagenase (Worthington Biochemical Co., Lakewood, NJ, USA), 2 mg/ml bovine serum albumin (Sigma, St. Louis, MO, USA), 2 mg/ml trypsin inhibitor (Sigma), and 0.27 mg/ml ATP. Cells were plated onto sterile glass coverslips coated with poly l-lysine (2.5 μg/ml, Sigma) in 35 mm culture dishes. The cells were then cultured at 37 °C in a 95 % O2/5 % CO2 incubator in smooth muscle growth medium (SMGM, Clonetics Corp., San Diego, CA, USA), supplemented with 1 % antibiotics/antimycotics (Gibco, Grand Island, NY, USA) and 5 ng/ml murine stem cell factor (SCF, Sigma).
Patch-clamp experiments
The patch-clamp technique was tested using ICCs that showed the network-like structures in culture (2–3 days). The whole-cell configuration of the patch clamp technique was used to record membrane potentials (current clamp). Membrane potentials were amplified by Axopatch 200B (Axon Instruments, Foster, CA, USA). Command pulse was applied using an IBM-compatible personal computer and pClamp software (version 9.2, Axon Instruments). The data were filtered at 5 kHz and displayed on an oscilloscope and a computer monitor. Results were analyzed using Clampfit program (Axon Instruments) and GraphPad Prism software (version 2.01, GraphPad Software Inc., San Diego, CA, USA). All experiments pertaining to patch clamp were performed at 30 °C.
Collection of ICCs and RT-PCR
Cells which had the morphology of ICCs, triangular or spindle shapes with multiple branches, were collected (about 2–5 cells) by applying negative pressure to a cell in contact with a recording pipette, lifting the cell out of the bath, and immediately expelling the cell from the pipette by applying positive pressure into a 1.5 ml tube which contained Phosphate-buffered saline. The cells were spun down at 3000 rpm for 5 min and then lysis buffer was added. Total RNA was isolated using TRIzol reagent according to the manufacturer specifications (Invitrogen, CA, USA). cDNAs were produced from the total RNA using Superscript One-Step RT-PCR with Platinum Taq (Invitrogen). The primers used were as follows: HCN1 (Accession number NM_010408), forward 5′-GCCATGCTGAGCAAGCTGAGATTT-3′, reverse 5′-TCCGATCGAGTCGGTCAATAGCAA-3′ (with a product size of 325 bp); HCN2 (Accession number NM_008226), forward 5′-CACAGCCATGCTGACAAAGCTCAA-3′, reverse 5′-ATCTAGCCGGTCAATAGCCACAGT-3′ (with a product size of 325 bp); HCN3 (Accession number NM_008227), forward 5′-TCTGCAGTTTCTGGTCCCTATGCT-3′, reverse 5′-ACTGCTCCACCTGCTTGTACTTCT-3′ (with a product size of 308 bp); HCN4 (Accession number NM_001081192), forward 5′-ATCGTGGTGGAGGACAACACAGAAT-3′, reverse 5′-GACACAGCAGAAGCATCATGCCAA-3′ (with a product size of 337 bp); c-Kit (Accession number Y00864), forward 5′-GAGCCTTCCTGTGACAGTTCAAAT-3′, reverse 5′-TCTATTCTTGCGGATCTCCTCTTG-3′ (with a product size of 101 bp); Ano1 (Accession number NM_178642), forward 5′-AGGCCAAGTACAGCATGGGTATCA-3′, reverse 5′-AGTACAGGCCAACCTTCTCACCAA-3′ (with a product size of 213 bp); Myosin (Accession number NM_013607.2), forward 5′-GAGAAAGGAAACACCAAGGTCAAGC-3′, reverse 5′-AACAAATGAAGCCTCGTTTCCTCTC-3′ (with a product size of 233 bp); and PGP9.5 (Accession number NM_011670), forward 5′-GCCAACAACCAAGACAAGCTGGAA-3′, reverse 5′-GCCGTCCACGTTGTTGAACAGAAT-3′ (with a product size of 213 bp). The thermal cycler was programmed such that cDNA synthesis was followed immediately by PCR amplification. The cDNA synthesis was carried out at 45 °C for 30 min for the reverse transcription reaction followed by 94 °C for 5 min for the denaturation of the cDNA hybrid. The three-step cycling process was carried out as follows for 38 cycles: 94 °C for 30 s for denaturation, 60 °C for 30 s for annealing, and 72 °C for 30 s for the extension. The PCR products were visualized using 2 % agarose gel electrophoresis followed by ethidium bromide staining.
Measurement of intracellular Ca2+ concentration
Fluo-3/AM, which was initially dissolved in dimethyl sulfoxide and stored at −20 °C, was used for monitoring changes in the intracellular Ca2+ ([Ca2+]i) concentration. Cultured ICCs on coverslips (25 mm) were rinsed twice using bath solution (recipe below), followed by incubation in bath solution containing 1 μM fluo-3/AM with 5 % CO2 at 37 °C for 5 min, and were then rinsed 2 more times with bath solution. They were then mounted on a perfusion chamber and scanned every 0.4 s using a Nikon Eclipse TE200 inverted microscope equipped with a Perkin-Elmer Ultraview confocal scanner and a Hamamatsu Orca ER 12-bit CCD camera (200×). Fluorescence excitation occurred at a wavelength of 488 nm and the emitted light was observed at 515 nm. The temperature of the perfusion chamber containing the cultured ICC was maintained at 30 °C during the scanning of Ca2+ imaging. Variations of [Ca2+]i fluorescence emission intensity were expressed as F1/F0 where F0 is the intensity of the first imaging.
Reagents
The cells were bathed in bath solution containing (mM): KCl 5, NaCl 135, CaCl2 2, glucose 10, MgCl2 1.2 and HEPES 10 and was adjusted to pH 7.4 with Tris. The pipette solution contained 140 mM KCl, 5 mM MgCl2, 2.7 mM K2ATP, 0.1 mM Na2GTP, 2.5 mM creatine phosphate disodium, 5 mM HEPES, and 0.1 mM EGTA, adjusted to pH 7.2 with Tris. The drugs used were: tetrodotoxin, nicardipine, SQ-22536, dideoxyadenosine, rolipram, 8-bromo-cAMP, CsCl, ZD7288, zatebradine, clonidine, genistein, l-cis-diltiazem, KT-5720, and 8-pCPT-2′-O-Me-cAMP. ZD7288 and zatebradine were purchased from Calbiochem (San Diego, CA, USA) and the other compounds were purchased from Sigma.
Statistical analysis
Data are expressed as the means ± standard errors. Differences in the data were evaluated by the Student’s t test. A p value <0.05 was considered to indicate a statistically significant difference. The n values reported in the text refer to the number of cells used in the patch clamp experiments.
Results
Involvement of cAMP production in generating pacemaker potentials in ICCs
The patch clamp technique was tested with ICCs that had network-like structures in culture (2–3 days). In the current clamp mode, the ICCs generated pacemaker potentials (Fig. 1a). Under control conditions in the current clamp mode, the resting membrane potential, frequency and amplitude were −58.7 ± 2.5 mV, 7.9 ± 2.6 cycles/5 min, and 41.4 ± 7.9 mV, respectively (n = 23; Fig. 1a, d, e). To evaluate whether voltage-dependent Na+ and Ca2+ channels were involved in generating pacemaker potentials, we first treated with tetrodotoxin (TTX, 1 μM; n = 10) and nicardipine (1 μM; n = 9). We found that the resting membrane potential, frequency, and amplitude of pacemaker potentials were not changed by TTX and nicardipine (Fig. 1b, c), indicating that voltage-dependent Na+ and L-type Ca2+ channels were not involved in generating pacemaker potentials. Since HCN channels were activated directly by cAMP, we next examined the involvement of cAMP using SQ-22536 (100 μM; n = 6), dideoxyadenosine (100 μM; n = 6) (both adenylate cyclase inhibitors), rolipram (a cAMP-specific phosphodiesterase inhibitor, 100 μM; n = 7), and cell-permeable 8-bromo-cAMP (100 μM; n = 8). Both SQ-22536 and dideoxyadenosine decreased the frequency of pacemaker potentials and hyperpolarized the membrane. In contrast, rolipram and cell-permeable 8-bromo-cAMP increased the frequency of pacemaker potentials and depolarized the membrane (Fig. 2a–d). The values of the frequency induced by above drugs were significantly different from the control values (Fig. 2f). However, the values of the resting membrane potential induced by these drugs were not significantly different from control values even though they changed membrane potentials (Fig. 2e). These results suggest that basal intracellular cAMP plays an important role in regulating the pacemaker frequency in colonic ICCs.
The cellular action of cAMP is mediated mainly by protein kinase A (PKA) or cAMP-regulated guanine nucleotide exchange factor (Epac) [27]. Thus, to assess whether basal cAMP action was mediated by either PKA or Epac, we examined the effect of KT-5720 (a PKA inhibitor, 10 μM; n = 7) or 8-pCPT-2′-O-Me-cAMP (an Epac agonist, 100 μM; n = 7), and found that both KT-5720 and Epac agonist had no effects on pacemaker activity (Fig. 3a, b). Since cyclic nucleotide gated (CNG) channel is another downstream target of cAMP [28], we treated with l-cis-diltiazem, a CNG channel blocker (10 μM; n = 7). We found that l-cis-diltiazem also had no effect on pacemaker activity (Fig. 3c). The values of the resting membrane potential and frequency induced by the above drugs were not significantly different from control values (Fig. 3d, e). These results suggest that cAMP-dependent regulation of pacemaker frequency was not mediated by PKA, Epac or CNG channels.
The effects of HCN channel blockers on pacemaker activity in cultured colonic ICCs
To evaluate whether the cAMP-dependent regulation of pacemaker activity was mediated through HCN channels, we treated ICCs with HCN channel blockers such as CsCl (5 mM; n = 6), ZD7288 (10 μM; n = 8), zatebradine (10 μM; n = 8), and clonidine (100 μM; n = 6) [29]. In addition, there is a report that Src tyrosine kinase inhibitor, genistein, modulates HCN channels [30], we also treated genistein (10 μM; n = 5) to pacemaker potential generating colonic ICCs. We found that CsCl, ZD7288, zatebradine, clonidine, and genistein all decreased the frequency of pacemaker potentials (Fig. 4a–e). The responses to the above drugs on the pacemaker potentials in ICCs are summarized in Fig. 4f and g. The values of the frequency induced by the above drugs were significantly different from control values. These results suggest that the cAMP-dependent regulation of pacemaker activity may be mediated through HCN channels.
The expression of HCN1 and HCN3 channels in colonic ICCs
To support the above functional data, we performed RT-PCR to detect expression of HCN channels. There are four subtypes of the HCN channels: HCN1–HCN4. In this study, we used mouse heart and HEK-293 cells as positive and negative controls for HCN channels (n = 4; Fig. 5a). RT-PCR analysis revealed the mRNA transcripts for all four HCN channel subtypes in whole mount cultured colonic cells (n = 12; Fig. 5b). However, the mRNA transcripts for only HCN1 and HCN3 channel were detected in cultured c-kit and Ano1 positive isolated ICCs (n = 14; Fig. 5c).
The effects of cAMP-related drugs and HCN channel blockers on [Ca2+]i oscillations of ICCs
In cultured intestinal ICCs, the activity of pacemaker channels was found to be closely coupled with spontaneous [Ca2+]i oscillations [31]. Thus, to evaluate whether HCN channels activation is coupled with spontaneous [Ca2+]i oscillations, we measured spontaneous [Ca2+]i oscillations in cultured colonic ICCs that are connected with cell cluster and treated with 8-bromo-cAMP (100 μM; n = 6), rolipram (100 μM; n = 6), SQ-22536 (100 μM; n = 6), CsCl (5 mM; n = 6), ZD7288 (10 μM; n = 6), and genistein (10 μM; n = 4). ICCs were loaded with fluo3-AM and spontaneous [Ca2+]i oscillations were observed in a time series. The frequency of [Ca2+]i oscillations was 7.3 ± 1.6 cycles/5 min in the control (n = 34). Both 8-bromo-cAMP and rolipram increase spontaneous [Ca2+]i oscillations (Fig. 6a, b). In contrast, SQ-22536, CsCl, ZD7288, and genistein all decreased spontaneous [Ca2+]i oscillations (Fig. 6c–f). The values of the frequency induced by the above drugs were significantly different from control values. These results suggested that the basal activation of HCN channels may be coupled with spontaneous [Ca2+]i oscillations (Fig. 6g).
HCN channels were not involved in the generation of pacemaker potentials in intestinal ICCs
To evaluate whether HCN channels also involve in intestinal pacemaking, we treated intestinal ICCs with SQ-22536 (100 μM; n = 5), rolipram (100 μM; n = 5), 8-bromo-cAMP (100 μM; n = 9), CsCl (5 mM; n = 6), ZD7288 (10 μM; n = 5), zatebradine (10 μM; n = 5), clonidine (100 μM; n = 5), and genistein (10 μM; n = 6) and performed RT-PCR. We found that all the drugs had no effect on pacemaker potentials of intestinal ICCs (Fig. 7a–h). In RT-PCR analysis, the mRNA transcripts for four HCN channel subtypes were detected in whole-mount cultured intestinal cells (n = 6; Fig. 8a). However, none of the HCN channel subtypes were expressed in cultured c-kit and Ano1 positive intestinal ICCs (n = 10; Fig. 8b).
Discussion
In the present study, our data suggest that tonically activated HCN channels participate in regulating pacemaker activity in colonic ICCs. The major findings of this study are that basal intracellular cAMP production maintains the resting pacemaker activity in colonic ICCs and that HCN channel blockers inhibited the pacemaker activity and decrease intracellular Ca2+ oscillations. In addition, we observed that the expression of HCN channels by RT-PCR in colonic ICCs. To our knowledge, the findings of this study are the first to suggest that HCN channels may be present in colonic ICCs and regulate pacemaker activity.
HCN channels are a family of non-selective cationic channels that conduct Na+ and K+. These channels are comprised of four subtypes (HCN1–HCN4) and each subtype has six transmembrane domains containing intracellular N- and C-terminus. HCN channels are activated by hyperpolarization of the membrane and by direct binding of intracellular cAMP to a cyclic nucleotide-binding domain (CNBD) on the C-terminus [32]. Neurotransmitters can influence firing rates of active spontaneous cells by either increasing or decreasing cAMP levels through HCN channels. Cardiac sinoatrial cells have I f (funny)-currents that regulate heart rate mediated by HCN channels. Sympathetic β-adrenoceptor stimulation increases intracellular cAMP level and leads to increased f-channel activity followed by increased heart rate. Whereas parasympathetic muscarinic receptor stimulation decreases intracellular cAMP level and leads to a decrease in f-channel activity followed by decreased heart rate [33].
We thought the possibilities that HCN channels can exist in ICCs because ICCs are also active pacemaker cells. Thus, we were curious whether the pacemaker activity in colonic ICCs is regulated by intracellular cAMP. The intracellular cAMP level depends on the activity of two groups of enzyme: the adenylate cyclase that produces cAMP and the phosphodiesterase (PDE) that hydrolyzes cAMP [34, 35]. In this study, SQ-22536 and dideoxyadenosine, both adenylate cyclase inhibitors, decreased the frequency of pacemaker potentials in colonic ICCs. In contrast, rolipram, a specific cAMP-dependent PDE 4 inhibitor, and a cell-permeable 8-bromo-cAMP both increased the frequency of pacemaker potentials. These results strongly suggest that basal intracellular cAMP production maintains the resting pacemaker frequency in colonic ICCs. cAMP is a key second messenger that transmits information to many different effector proteins within the cell. The cellular action of cAMP is mediated by PKA or Epac. However, KT-5720, a PKA inhibitor, or 8-pCPT-2′-O-Me-cAMP, an Epac agonist had no effects on pacemaker activity, indicating that the regulatory effect of cAMP on pacemaker frequency in colonic ICCs is direct rather than being mediated by protein phosphorylation. This finding suggests that another pathway of cAMP-mediated regulation of pacemaker activity exist in colonic ICCs. CNG and HCN channels are other downstream target proteins of cAMP [36]. However, l-cis-diltiazem, a CNG channel blocker, did not change the pacemaker potentials. Thus, we postulated that HCN channels may be a target protein of cAMP for regulation of pacemaker activity in colonic ICCs. To support this hypothesis, we performed pharmacological studies. Pharmacologically, Cs+, ZD7288 and zatebradine are HCN channel blockers that are commonly used in identification of HCN channels in various cells [37]. It has also been reported that clonidine, a α2-adrenoceptor agonist, directly blocks the HCN channels activity in cardiac sinoatrial cells [38]. Regulation of HCN channel by tyrosine kinases of the Src family has been also reported in heart and neuron [39]. In this study, HCN channel blockers and the tyrosine kinase inhibitor, genistein suppressed pacemaker frequency in colonic ICCs. These above results suggest that HCN channels are under tonically activated by basal cAMP in colonic ICCs. The similar results have been reported in spontaneous firing GH3 cells where basal cAMP tonically activates HCN channels [40].
To confirm and support the functional data, we performed RT-PCR in colonic ICCs in cultured cells. The distribution of HCN channel subunits was both regional- and species-dependent. In the central nervous system, HCN1 channel was found to be expressed mainly in the photoreceptors, neocortex, and cerebellar cortex. HCN2 channel was expressed in nearly all brain tissues. HCN3 channel was found in the olfactory bulb and HCN4 channel was found in the thalamus and olfactory bulb. In the heart, all four HCN channel subunits are detected [41]. In sinoatrial nodes, the HCN4 channel is the major subunit in all species and the remaining small fraction of these channels is species dependent. HCN1 channel is dominant in rabbit, whereas the HCN2 channel is dominant in mouse and human [42]. In the GI tract of mice, enteric neurons expressed HCN1, HCN2, and HCN4 channels but not HCN3 channels [43]. Recently, Yang et al. [44] investigated the role HCN2 channels on spontaneous rhythmic activity of GI tract in mice. However, immunoreactivity of the anti-HCN2 antibody was only detected in myenteric neurons and not in ICCs. In our study, RT-PCR analysis revealed the presence of mRNA transcripts for all four HCN channels subtypes in the whole mount cultured colonic cells. However, in c-kit and Ano1 positive colonic ICCs, only mRNA transcripts for the HCN1 channel and HCN3 channel were expressed. Taken together these results suggest that ICCs also have HCN channels and have regional differences in distribution of HCN channels in mouse.
The pacemaking mechanism in intestinal ICCs is related to IP3-dependent intracellular Ca2+ oscillations that are coupled with the periodic activation of pacemaker channels. In this study, intracellular Ca2+ oscillations are increased by rolipram or 8-bromo-cAMP and decreased by SQ-22536 or dideoxyadenosine in colonic ICCs. In addition, HCN channel blockers suppressed spontaneous intracellular Ca2+ oscillations. This suggests the activation of HCN channels and intracellular Ca2+ oscillations are also closely coupled to generate pacemaker activity.
Finally we also performed pharmacological experiments and RT-PCR on intestinal ICCs to compare with the results from colonic ICCs. In contrast to colonic ICCs, all cAMP-related drugs and HCN channel blockers had no effect on the intestinal pacemaker frequency. HCN channels were not detected in the intestinal ICCs by RT-PCR. These results strongly suggest that there are regional differences in HCN channels and that different pacemaking mechanisms may exist between the small intestine and the colon.
In summary, periodically activated HCN channels by basal intracellular cAMP production are present in colonic ICCs and they regulate pacemaker activity. The different regional distribution in HCN channels between the intestine and colon suggest a cause for differential regulation of GI motility. Our results suggest that HCN channel may participate in regulation of pacemaking activity and may be an effective therapeutic target for abnormal colonic motility disorders. However, because our study was performed in cultured cells, further studies are require to confirm the existence of HCN channels and elucidate the fine precise mechanisms in native tissues.
References
Thomsen L, Robinson TL, Lee JC, Farraway LA, Hughes MJ, Andrews DW, et al. Interstitial cells of Cajal generate a rhythmic pacemaker current. Nat Med. 1998;4:848–51.
Ward SM, Sanders KM, Hirst GD. Role of interstitial cells of Cajal in neural control of gastrointestinal smooth muscles. Neurogastroenterol Motil. 2004;16:112–7.
Won KJ, Sanders KM, Ward SM. Interstitial cells of Cajal mediate mechanosensitive responses in the stomach. Proc Natl Acad Sci USA. 2005;102:14913–8.
Szurszewski JH. Electrical basis for gastrointestinal motility. In: Johnson LR, editor. Physiology of the gastrointestinal tract. New York: Raven Press; 1987. p. 383–422.
Der-Silaphet T, Malysz J, Hagel S, Larry Arsenault A, Huizinga JD. Interstitial cells of cajal direct normal propulsive contractile activity in the mouse small intestine. Gastroenterology. 1998;114:724–36.
Ward SM, Ordog T, Koh SD, Baker SA, Jun JY, Amberg G, et al. Pacemaking in interstitial cells of Cajal depends upon calcium handling by endoplasmic reticulum and mitochondria. J Physiol. 2000;525:355–61.
Malysz J, Donnelly G, Huizinga JD. Regulation of slow wave frequency by IP(3)-sensitive calcium release in the murine small intestine. Am J Physiol Gastrointest Liver Physiol. 2001;280:G439–48.
Sanders KM, Ördög T, Koh SD, Ward SM. A novel pacemaker mechanism drives gastrointestinal rhythmicity. News Physiol Sci. 2000;15:291–8.
Walker RL, Koh SD, Sergeant GP, Sanders KM, Horowitz B. TRPC4 currents have properties similar to the pacemaker current in interstitial cells of Cajal. Am J Physiol Cell Physiol. 2002;283:C1637–45.
Kim BJ, Lim HH, Yang DK, Jun JY, Chang IY, Park CS, et al. Melastatin-type transient receptor potential channel 7 is required for intestinal pacemaking activity. Gastroenterology. 2005;129:1504–17.
Tokutomi N, Maeda H, Tokutomi Y, Sato D, Sugita M, Nishikawa S, et al. Rhythmic Cl− current and physiological roles of the intestinal c-kit-positive cells. Pflűgers Arch. 1995;431:169–77.
Huizinga JD, Zhu Y, Ye J, Molleman A. High-conductance chloride channels generate pacemaker currents in interstitial cells of Cajal. Gastroenterology. 2002;123:1627–36.
Gomez-Pinilla PJ, Gibbons SJ, Bardsley MR, Lorincz A, Pozo MJ, Pasricha PJ, et al. Ano1 is a selective marker of interstitial cells of Cajal in the human and mouse gastrointestinal tract. Am J Physiol Gastrointest Liver Physiol. 2009;296:G1370–81.
Hwang SJ, Blair PJ, Britton FC, O’Driscoll KE, Hennig G, Bayguinov YR, et al. Expression of anoctamin 1/TMEM16A by interstitial cells of Cajal is fundamental for slow wave activity in gastrointestinal muscles. J Physiol. 2009;587:4887–904.
Caputo A, Caci E, Ferrera L, Pedemonte N, Barsanti C, Sondo E, et al. TMEM16A, a membrane protein associated with calcium-dependent chloride channel activity. Science. 2008;322:590–4.
Schroeder BC, Cheng T, Jan YN, Jan LY. Expression cloning of TMEM16A as a calcium-activated chloride channel subunit. Cell. 2008;134:1019–29.
Yang YD, Cho H, Koo JY, Tak MH, Cho Y, Shim WS, et al. TMEM16A confers receptor-activated calcium-dependent chloride conductance. Nature. 2008;455:1210–5.
Stanich JE, Gibbons SJ, Eisenman ST, Bardsley MR, Rock JR, Harfe BD, et al. Ano1 as a regulator of proliferation. Am J Physiol Gastrointest Liver Physiol. 2011;301:G1044–51.
Nakayama S, Torihashi S. Spontaneous rhythmicity in cultured cell clusters isolated from mouse small intestine. Jpn J Physiol. 2002;52:217–27.
Kito Y, Suzuki H. Effects of temperature on pacemaker potentials in the mouse small intestine. Pflűgers Arch. 2007;454:263–75.
Huizinga JD, Farraway L, Den Hertog A. Effect of voltage and cyclic AMP on frequency of slow-wave-type action potentials in canine colon smooth muscle. J Physiol. 1991;442:31–45.
Liu LW, Thuneberg L, Huizinga JD. Cyclopiazonic acid, inhibiting the endoplasmic reticulum calcium pump, reduces the canine colonic pacemaker frequency. J Pharmacol Exp Ther. 1995;275:1058–68.
Sanders KM. G protein-coupled receptors in gastrointestinal physiology IV. Neural regulation of gastrointestinal smooth muscle. Am J Physiol. 1998;275:G1–7.
DiFrancesco D. The contribution of the ‘pacemaker’ current (if) to generation of spontaneous activity in rabbit sino-atrial node myocytes. J Physiol. 1991;434:23–40.
Pape HC. Queer current and pacemaker: the hyperpolarization-activated cation current in neurons. Annu Rev Physiol. 1996;58:299–327.
DiFrancesco D, Tortora P. Direct activation of cardiac pacemaker channels by intracellular cyclic AMP. Nature. 1991;351:145–7.
de Rooij J, Zwartkruis FJ, Verheijen MH, Cool RH, Nijman SM, Wittinghofer A, et al. Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP. Nature. 1998;396:474–7.
Kaupp UB, Seifert R. Cyclic nucleotide-gated ion channels. Physiol Rev. 2002;82:769–824.
Baruscotti M, Bucchi A, Difrancesco D. Physiology and pharmacology of the cardiac pacemaker (“funny”) current. Pharmacol Ther. 2005;107:59–79.
Zong X, Eckert C, Yuan H, Wahl-Schott C, Abicht H, Fang L, et al. A novel mechanism of modulation of hyperpolarization-activated cyclic nucleotide-gated channels by Src kinase. J Biol Chem. 2005;280:34224–32.
Sanders KM, Koh SD, Ward SM. Interstitial cells of Cajal as pacemakers in the gastrointestinal tract. Annu Rev Physiol. 2006;68:307–43.
Biel M, Wahl-Schott C, Michalakis S, Zong X. Hyperpolarization-activated cation channels: from genes to function. Physiol Rev. 2009;89:847–85.
Verkerk AO, van Ginneken AC, Wilders R. Pacemaker activity of the human sinoatrial node: role of the hyperpolarization-activated current, I(f). Int J Cardiol. 2009;132:318–36.
Sunahara RK, Dessauer CW, Gilman AG. Complexity and diversity of mammalian adenylyl cyclases. Annu Rev Pharmacol Toxicol. 1996;36:461–80.
Beavo JA. Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms. Physiol Rev. 1995;75:725–48.
Craven KB, Zagotta WN. CNG and HCN channels: two peas, one pod. Annu Rev Physiol. 2006;68:375–401.
Robinson RB, Siegelbaum SA. Hyperpolarization-activated cation currents: from molecules to physiological function. Annu Rev Physiol. 2003;65:453–80.
Knaus A, Zong X, Beetz N, Jahns R, Lohse MJ, Biel M, et al. Direct inhibition of cardiac hyperpolarization-activated cyclic nucleotide-gated pacemaker channels by clonidine. Circulation. 2007;115:872–80.
Yu HG, Lu Z, Pan Z, Cohen IS. Tyrosine kinase inhibition differentially regulates heterologously expressed HCN channels. Pflűgers Arch. 2004;447:392–400.
Kretschmannova K, Gonzalez-Iglesias AE, Tomić M, Stojilkovic SS. Dependence of hyperpolarisation-activated cyclic nucleotide-gated channel activity on basal cyclic adenosine monophosphate production in spontaneously firing GH3 cells. J Neuroendocrinol. 2006;18:484–93.
Herrmann S, Stieber J, Ludwig A. Pathophysiology of HCN channels. Pflűgers Arch. 2007;454:517–22.
Marionneau C, Couette B, Liu J, Li H, Mangoni ME, Nargeot J, et al. Specific pattern of ionic channel gene expression associated with pacemaker activity in the mouse heart. J Physiol. 2005;562:223–34.
Xiao J, Nguyen TV, Ngui K, Strijbos PJ, Selmer IS, Neylon CB, et al. Molecular and functional analysis of hyperpolarisation-activated nucleotide-gated (HCN) channels in the enteric nervous system. Neuroscience. 2004;129:603–14.
Yang S, Xiong CJ, Sun HM, Li XS, Zhang GQ, Wu B, et al. The distribution of HCN2-positive cells in the gastrointestinal tract of mice. J Anat. 2012;221:303–10.
Acknowledgments
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) and funded by the Ministry of Education, Science and Technology [2012-0001335].
Conflict of interest
The authors disclose no conflicts.
Author information
Authors and Affiliations
Corresponding author
Additional information
P. K. Shahi and S. Choi contributed equally to this work.
Rights and permissions
Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
About this article
Cite this article
Shahi, P.K., Choi, S., Zuo, D.C. et al. The possible roles of hyperpolarization-activated cyclic nucleotide channels in regulating pacemaker activity in colonic interstitial cells of Cajal. J Gastroenterol 49, 1001–1010 (2014). https://doi.org/10.1007/s00535-013-0849-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00535-013-0849-3