Abstract
The seasonal savannas (cerrados) of Central Brazil are characterized by a large diversity of evergreen and deciduous trees, which do not show a clear differentiation in terms of active rooting depth. Irrespective of the depth of the root system, expansion of new foliage in deciduous species occurs at the end of the dry season. In this study, we examined a suite of leaf traits related to C assimilation, water and nutrients (N, P) in five deciduous and six evergreen trees that were among the dominant families of cerrado vegetation. Maximum CO2 assimilation on a mass basis (Amass) was significantly correlated with leaf N and P, and specific leaf area (SLA; leaf area per unit of leaf mass). The highest leaf concentrations of both nutrients were measured in the newly mature leaves of deciduous species at the end of the dry period. The differences in terms of leaf N and P between evergreen and deciduous species decreased during the wet season. Deciduous species also invested less in the production of non-photosynthetic leaf tissues and produced leaves with higher SLA and maintained higher water use efficiency. Thus, deciduous species compensated for their shorter leaf payback period by maintaining higher potential payback capacity (higher values of Amass) and lower leaf construction costs (higher SLA). Their short leafless period and the capacity to flush by the end of the dry season may also contribute to offset the longer payback period of evergreen species, although it may involve the higher cost of maintaining a deep-root system or a tight control of plant water balance in the shallow-rooted ones.
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Introduction
The central plains of Brazil are occupied by a complex of seasonal savannas, known locally as “cerrado.” The cerrados of central Brazil are the second most extensive plant formation in South America and cover 2.0×106 km2 of land area (Eiten 1972). Like other Neotropical savannas, the cerrado is characterized by strong rainfall seasonality, coupled with constantly high diurnal air temperatures. The soils are generally deep and well drained. Superficial soil layers dry out during the dry season that extends from May to September, while deeper soil layers exhibit a much higher degree of water constancy throughout the year (Franco 2002). The nutrient-poor, acid soils represent an additional limiting factor for plant growth in this region. P is particularly limiting in these ecosystems (Haridasan 2000, 2001).
The upper canopy of cerrado vegetation typically consists of 6- to 8-m-tall, deciduous and evergreen trees (Eiten 1972). The coexistence of tree species with contrasting phenological behaviors in savanna ecosystems has been taken as an indication of differences in depth of the root systems, whereby drought-deciduous species are expected to have more superficial root systems and a tight stomatal regulation of transpiration. Deep-rooted evergreen trees would be able to supply enough water to maintain high rates of transpiration and CO2 assimilation during the dry period (Goldstein et al. 1989). On the other hand, one could expect that deciduous species should have a less conservative strategy for water use because of the short payback interval (short leaf life-span), while evergreen species should have a more conservative strategy of water use (Lloyd and Farquhar 1994). Deciduous species could overcome this limitation if they are able to sustain higher photosynthetic rates per unit of water loss in relation to evergreen species. However, evergreen and deciduous cerrado trees may not show such contrasting strategies of water use and leaf C balance in response to the seasonal drought. Comparisons of hydrogen isotope composition of xylem water and soil water samples collected at different depths do not suggest a clear differentiation in terms of active rooting depth between deciduous and evergreen trees in the Cerrado (Table 1; Jackson et al. 1999). High atmospheric evaporative demand and hydraulic constraints result in strong stomatal limitation of transpiration and a prolonged midday depression of photosynthetic rates in both evergreen and deciduous species during both the wet and the dry seasons (Johnson et al. 1983; Franco 1998; Moraes and Prado 1998; Meinzer et al. 1999; Naves-Barbiero et al. 2000; Franco and Lüttge 2002). Indeed, evergreen and deciduous cerrado trees showed similar stomatal responses to changes in air saturation deficit (Bucci et al. 2004). Moreover, many deciduous trees remain leafless for only short periods, and are able to flush new leaves during the dry season (Rivera et al. 2002).
Photosynthesis is strongly affected by N availability. This reflects a combination of the larger proportional investment of N in photosynthetic machinery and the widespread N limitation in natural ecosystems. Studies in a range of ecosystems have shown that deciduous species generally have higher N concentrations per unit leaf mass and photosynthetic rates than evergreen species (Mooney and Gulmon 1982; Field and Mooney 1986; Sobrado 1991, 1994; Eamus and Cole 1997; Reich et al. 1992, 1997). On the other hand, some field studies have reported that photosynthesis is more limited by P availability in P-deficient soils (Reich and Schoettle 1988; Cordell et al. 2001). A detailed assessment of the nutrient relations of the selected species was beyond the scope of this study. However, measurements of leaf N and P content and specific leaf area (SLA; leaf area per unit of leaf mass) coupled with gas exchange measurements allowed us to address questions related to leaf C balance, patterns of leaf phenology and nutrient allocation in cerrado trees. We also measured seasonal changes in leaf δ13C, N and P, predawn and midday leaf water potential, as well as patterns of leaf phenology and leaf life-span. Foliar δ13C values of plants obtaining their CO2 from the same sources are considered to be an integrated, long-term estimate of intrinsic water use efficiency (WUE) (Ehleringer and Cooper 1988; Meinzer et al. 1990). Lower values of SLA generally reflect more investment of N in non-photosynthetic components (Field and Mooney 1986). Deciduous species are constrained by the shorter leaf life-span. Thus, we expected that deciduous species would invest less in the production of non-photosynthetic tissues and would produce leaves with higher SLA and higher N and P concentrations. Deciduous species should also have higher photosynthetic rates and WUE than evergreen species, because of the well-known linear relationship between photosynthesis and leaf N and the close correlation between N and P concentrations.
Materials and methods
Study site, plant material and leaf phenology
The study was carried out at the ecological reserve of the Instituto Brasileiro de Geografia e Estatística (IBGE), located approximately 33 km south of Brasilia. The study site (15°56′S, 47°53′W, altitude 1,100 m) is a seasonal savanna having a relatively continuous layer of perennial grasses with a discontinuous layer of deciduous and evergreen trees with a low and almost shrubby growth form. Most trees and shrubs have a thick bark, twisted trunks and scleromorphic leaves. Soils are deep, well-drained, strongly acid dystrophic latosols, with high Al content. The climate is Aw by Köppen’s classification and according to the weather station records of the IBGE ecological reserve, the average rainfall is 1,483 mm (1980–1997) with a distinct dry season from May to September. The dry season of 1995 was particularly severe. Although annual rainfall was 1,684 mm, it rained only 37 mm from May to September. Annual rainfall was 1,384 mm in 1996, but it rained a total of 112 mm from May to September. Average (diurnal) relative humidity is around 80% during the rainy season and drops to 55% during the dry season when daily minimum relative humidity reaches values as low as 15%. Mean annual temperature is about 22°C.
A total of 11 woody species were selected based on their phenology and abundance among dominant families of cerrado vegetation (Table 1). These species are commonly found throughout the cerrado domain. In a study of the floristic composition of 98 sites of cerrado and Amazonian savanna, encompassing most of the area of such vegetation in Brazil, these species occurred in 15% (Miconia ferruginata) to 82% (Qualea grandiflora) of the sites (Ratter et al. 1996).
Starting in September, 1995, branches or twigs of five individuals of each species were marked in such a way that individual leaves could be identified, their abscission noted and the formation of new mature leaves recorded. Five twigs or branches were marked on each individual where possible, since the growth habit of some species, with limited branching, provides few growing points. This was the case with Schefflera macrocarpa and Kielmeyera coriacea, for example. Monthly observations were carried out from September 1995 to November 1998. In order to define the duration of the leafless period, observations were made on a weekly basis from the end of June to the end of October, when four individuals of Dalbergia miscolobium were included, starting in June 1997. Based on these observations, pattens of leaf phenology were obtained, as well as estimates of leaf longevity.
Measurements of leaf gas-exchange and leaf water potential
Maximum CO2 assimilation (\(A_{\text{CO}_2 }\)), stomatal conductance at \(A_{\text{CO}_2 }\) (gwv), intrinsic WUE (\(A_{\text{CO}_2 }\)/gwv) and the ratio of intercellular to ambient CO2 partial pressure (pi/pa) were determined under ambient conditions with a portable photosynthesis system (301-PS CID, Vancouver, Wash.). The 301-PS was configured in the open system mode. Gas exchange measurements were carried out towards the end of the dry season (August) of 1996 and at the peak of the wet season (January and February) of 1997. Because of the small compound leaves, we used a LI-6200 (Li-Cor, Lincoln, Neb.) to measure gas exchange in Pterodon pubescens and D. miscolobium. These measurements were made only in January 1996 and problems with the humidity sensor prevented the calculation of gwv and pi/pa. Leaf gas exchange was measured in two or three sunlit leaves of four (Q. grandiflora in 1996) to six (all others) pre-marked individuals during a 2- to 4-h period between 0800 hours and 1200 hours.
predawn and midday leaf water potentials were used as indicators of plant water status. Leaf water potential was measured with a pressure chamber (model 3005-14442; Soil Moisture, Santa Barbara, Calif.) in August of 1996 and February of 1997. One leaf of three to six individuals was sampled at each time. Measurements were done in the same individuals used in the gas exchange measurements.
Isotopic and nutrient analysis
Several adult leaves per individual were selected and pooled by individual. The leaves were then transported to the laboratory where their area was determined. They were then oven-dried at 70°C and their dry leaf mass determined for calculation of SLA (ratio of leaf area to leaf dry mass). The dried leaves were finely ground and a subsample was taken for total N and P determinations. N was determined in diluted acid digests according to the micro-Kjeldhal procedure (Bataglia et al. 1978). Total extractable P was determined colorimetrically by complex formation with molibdovanadate according to Bataglia et al. (1978). An additional subsample was taken for δ13C determination. Samples were sent to Mountain Mass Spectrometry (Evergreen, Colo.), where the relative abundance of 13C and 12C in the CO2 produced from combustion of the samples was analyzed by mass spectrometry. C isotopic discrimination (Δ) was calculated as (δ13Cair–δ13Cplant)/(1+δ13Cplant), where δ13Cplant is the C isotope ratio of the plant material and δ13Cair is that of the air (−8‰), as described by Farquhar and Richards (1984).
Samples for C isotope and nutrient analysis were taken in September and November 1995, and March 1996. To relate leaf N and P to CO2 assimilation, additional samples for nutrient analysis were taken in January 1996 (P. pubescens and D. miscolobium) and January 1997 (all other species), when gas exchange measurements were performed.
Data analysis
Although we examined a large number of species, we recognise that we do not have enough species to clearly distinguish the four different phenological groups depicted in Table 1. Thus, we pooled briefly deciduous and deciduous species in a single functional group, as leaf life-span in all of them is <1 year and they remain leafless for at least several days. New leaves emerge after leaf abscission. The other six species were grouped as evergreens. They always maintain a green canopy and leaf life-span is at least 1 year. However, we kept all four phenological groups separated in most figures and tables and clear differences among them were highlighted.
The statistical package Statview for Windows 5.0 (SAS Institute) was used to analyze the data. A multivariate ANOVA (MANOVA) followed by the Fisher’s protected least significant difference multiple comparison test (Fisher’s) was applied to analyze the effects of leaf phenology and season on leaf N, P and Δ. Because the interaction term was significant, the variables were first log10 transformed to restore the additivity of the data (Sokal and Rohlf 1995). We did not have a complete C isotope data set for P. pubescens and Q. grandiflora. Thus, these two species were not included in the analyses. However, all the available data for these two species were included in the figures.
We used unpaired t-tests or Mann-Whitney U-tests to evaluate the differences between evergreen and deciduous in regard to SLA and \(A_{\text{CO}_2 }\) on a mass and leaf area basis and paired t-tests to evaluate the effect of drought on gas exchange parameters and leaf water potential of evergreen species. Significance was determined as P<0.05 unless otherwise noted.
Results and discussion
The timing of bud break was variable among evergreen species, while all deciduous species flushed by the end of the dry season (Table 1). Evergreen leaf-exchangers simultaneously shed the leaves and produced new ones, while the briefly deciduous species remained leafless for short periods of time of <3 weeks. The other deciduous species remained leafless for periods of about 3–6 weeks (Table 1). There was variation between the evergreen species with regard to leaf longevity. M. ferruginata retained its leaves for approximately 24 months, while Schefflera macrocarpa and Roupala montana tended to retain their leaves for approximately 18 months. Leaf life-span was about 12 months for the three evergreen leaf-exchangers and 10–11 months for the deciduous species. Although there was not a sharp separation in terms of active rooting depth between the two phenological groups in the dry season, most deciduous species were apparently extracting water from deeper soil layers than the evergreens (Table 1).
Deciduous and evergreen species had similar rates of \(A_{\text{CO}_2 }\) at the peak of the wet season (Table 1). gwvs were also similar between the two phenological types, ranging from 133 mmol m−2 s−1 to 228 mmol m−2 s−1. Differences in maximum CO2 assimilation and stomatal conductance between evergreen and deciduous trees are frequently small in savannas and dry deciduous forests (Goldstein et al. 1989; Sobrado 1991, 1994; Eamus and Cole 1997). Deciduous species invested less in the production of non-photosynthetic leaf tissues and produced leaves with higher SLA (t-test; P=0.016; Table 1). These differences were reflected in CO2 assimilation rates on a mass basis (Amass). Values of maximum Amass of deciduous and evergreen species averaged 92.9 μmol kg−1 s−1 and 62.7 μmol kg−1 s−1, respectively. These differences were statistically significant (t-test; P=0.019). Thus, deciduous species compensated their shorter leaf pay back period by an increase in the potential payback capacity (maximum Amass), when soil water was not limiting. Prado and Moraes (1997) reported Amass values within this range for 20 cerrado woody species at a site near the southern limit of cerrado vegetation, but they did not include information on leaf phenology. Amass increased with SLA for both evergreen and deciduous species (Fig. 1a). The relationship was similar to the general one fitted for data across biomes (Reich et al. 1999).
Photosynthesis is strongly affected by N availability and it is well known that photosynthetic capacity increases linearly with leaf N concentration (Field and Mooney 1986; Reich et al. 1995). Indeed, Eamus and Prior (2001) showed that, for a range of tree species from different seasonally dry ecosystems, leaf N increases with light-saturated \(A_{\text{CO}_2 }\), with no clear distinction between evergreen and deciduous species. This was also found in the present study. Maximum CO2 assimilation was significantly correlated with leaf N and leaf P on a mass basis (Fig. 1b, c) but not on an area basis (not shown). The close correlation between Amass and both leaf SLA and N suggests that decreasing SLA is associated with greater allocation of biomass to structural components of the leaf rather than metabolic components (Field and Mooney 1986; Reich et al. 1998). Indeed, using multiple linear regression, Amass was highly significantly related to the combination of leaf N and SLA (r=0.92, P=0.0006). As expected, there was also a significant correlation between leaf N and leaf P (Fig. 2) across species. Thus, deciduous and evergreen trees shared common relationships between leaf traits, which suggests that selective pressures impose strong constraints on functional trait variability in cerrado environments. On the other hand, the results of the MANOVA gives support to the hypothesis that evergreen and deciduous species have different combinations of leaf traits, at least in terms of leaf N, P and Δ and that these combinations are influenced by seasonality (Table 2).
Soil water availability is generally not a limiting factor during the wet season in cerrado ecosystems (Franco 2002). However, high atmospheric evaporative demand and hydraulic constraints impose a strong stomatal limitation of transpiration and a prolonged midday depression of photosynthesis during both the wet and the dry seasons (Franco 1998; Moraes and Prado 1998; Meinzer et al. 1999; Naves-Barbiero et al. 2000; Franco and Lüttge 2002). This would be particularly critical during the dry season, which is characterized by very low values of relative humidity (Franco 2002). Most evergreen species showed a decline in the maximum CO2 assimilation rates and stomatal conductances in the dry season, while leaf-to-air vapour pressure deficit (VPD) increased substantially (Fig. 3). The magnitude of the decrease was larger for stomatal conductance; as a result three species had a significant increase in \(A_{\text{CO}_2 }\)/gwv. On the other hand, pi/pa remained constant for five out of the six species. The only exception was Vochysia elliptica, where pi/pa showed a small, but significant increase by the end of the dry season. This deep-rooted species had the least variation in both predawn and midday leaf water potential (Fig. 4). Although statistically significant (t-test; P≤0.02), these seasonal differences in predawn and midday water potential were relatively small, when compared to trees of Venezuelan dry forests (Sobrado 1986) but similar to those of evergreen species of Australian savannas (Duff et al. 1997; Myers et al. 1997). Seasonal variations in predawn and midday water potential in evergreen cerrado species may partially reflect nocturnal transpiration that would limit plant recharge during night-time (Bucci et al. 2004).
We do not report measurements of gas exchange and of leaf water potential in deciduous species because they were leafless or had only very young leaves when we took the dry season measurements. However, other studies have reported that stomatal conductance in deciduous cerrado species also declined significantly during the dry season, while seasonal variations in leaf water potential were relatively small (Cardinot 1998; Maia 1999; Naves 2000; Bucci et al. 2004). This decrease in stomatal conductance in both phenological groups is mostly a response to the strong increase in air saturation deficit during the dry season (Bucci et al. 2004). Indeed, evergreen and deciduous cerrado species showed similar stomatal responses to changes in air saturation deficit (Bucci et al. 2004).
These seasonal changes in maximum \(A_{\text{CO}_2 }\) and gwv could affect WUE (CO2 assimilation/transpiration) of evergreen and deciduous species. Although WUE can be calculated from diurnal cycles of\(A_{\text{CO}_2 }\) and transpiration, we did not follow this approach because of the uncertainties involved in attempting to integrate instantaneous measurements to represent a whole season. Instead, we used changes in leaf Δ to assess WUE on a seasonal basis. Eleven of the 13 species studied by Mattos et al. (1997) in a cerrado area in southern Brazil showed a decrease in leaf Δ (higher WUE) during the dry season. The decrease ranged from 0.3‰ to 4‰ and it was statistically significant at the community level. They related their results to a larger decrease in gwv in comparison to\(A_{\text{CO}_2 }\). However, they based this conclusion on gas exchange results for only two of the 13 species. Although we were not able to detect a seasonal variation in leaf Δ at the community level, leaf Δ was significantly higher for deciduous species (Fig. 5a).
C isotope discrimination is largely dependent on the ratio of intercellular to ambient CO2 concentrations prevailing when the leaf C is assimilated (Farquhar et al. 1982, 1989). Thus a decrease in pi/pa should reflect diffusional limitations to CO2 uptake from reductions in stomatal conductance and/or an increase in carboxylation efficiency (higher CO2 assimilation rates; Ehleringer 1994). Higher foliar δ13C values were reported in shallow-rooted deciduous species of a Venezuelan seasonally tropical dry forest, when compared to foliar δ13C values of deep-rooted evergreen species (Sobrado and Ehleringer 1997). They related their results to a stronger stomatal control in the shallow-rooted deciduous species. On the other hand, Medina and Francisco (1994) observed an increase in foliar δ13C values toward the end of the dry season in both the evergreen Curatella americana and the deciduous Godmania macrocarpa in a Venezuelan savanna. They concluded that the similarity of WUE in both species were the result of higher photosynthetic capacity in G. macrocarpa and lower stomatal conductances in C. americana. The effects of drought on stomatal conductance are apparently similar for deciduous and evergreen cerrado trees (Bucci et al. 2004). Thus, differences between evergreen and deciduous cerrado trees in terms of WUE are probably the result of an increase in carboxylation efficiency, which could be achieved by producing new leaves with a higher N concentration. Indeed, there was a significant difference in leaf N and P between deciduous and evergreen species and a significant seasonal variation (Fig. 5b, c). The highest leaf N and P concentrations were measured in the newly mature leaves of deciduous plants that were produced at the peak of the dry season. It was also the period when the largest differences between deciduous and evergreen species were measured. The differences in terms of leaf N and P between the two phenological groups decreased during the wet season. There was also a significant relationship between leaf values of Δ and leaf N (r=0.41; P=0.026), as well as between Δ and P (r=0.48; P=0.007).
The use of leaf δ13C or Δ values as an integrated measure of WUE has to be examined with caution because it depends on assumption that CO2 concentration of the air (pa) and leaf-to-air VPD (ei − ea) will not change seasonally:
and
where a and b are 4.4‰ and 27‰, respectively (Farquhar et al. 1989).
Although one can perhaps assume small seasonal variations in pa, ei−ea increased substantially during the drought, from about 1.48 kPa to 2.40 kPa (Fig. 3). Deciduous species would still maintain higher WUE than evergreens at the end of the dry season, if we assume that both phenological groups would have similar values of leaf-to-air VPD.
One could still expect that C return per unit dry weight invested N and C should be higher in evergreens because leaves are photosynthetically active for longer periods (Sobrado 1991) and lower amortized leaf construction costs to replace the tree crown, provided that the leaves are held by more than a year (Givnish 2002). However, maximum C return in evergreens is greatly constrained by the combined effects of partial leaf loss and reductions in photosynthetic rates during the dry season (Franco 1998) and leaf damage by herbivores and pathogens (Marquis et al. 2002). Deciduous species and several evergreens flush leaves before the rains begin (Table 1), so that the most vulnerable stage of development (the new leaf stage) has already passed by the time the herbivorous insect activity begins (Marquis et al. 2002). However, leaf damage by pathogen attack is much higher than damage by herbivores in cerrado ecosystems and fully expanded leaves continued to accrue damage throughout their lives (Marquis et al. 2001, 2002). The lower SLA of the evergreen species also suggests larger leaf construction and maintenance costs and this should also be considered (Sobrado 1991; Eamus and Prior 2001).
Total daily water loss of deciduous and evergreen species showed little seasonal variation (Meinzer et al. 1999; Bucci et al. 2004). In the case of evergreens, partial leaf loss and partial stomatal closure was enough to compensate for the increase in evaporative demand of the air during the drought period. On the other hand, this would result in a significant decrease in daily C gain at the crown level. Thus, the effect of drought on plant WUE would be even more substantial, as the tree would not reduce water loss in the dry season, but it would fix less C. On the other hand, deciduous species would have to face the higher belowground costs associated with maintaining a deep-root system that characterizes most of them (Table 1). However, deciduous species remain completely leafless for only short periods (a few weeks to <2 months) and leaf flushing occurs primarily in the late dry season (Table 1; Maia 1999; Naves 2000; Rivera et al. 2002). This allows them to quickly achieve full crown development and maximal C gain when the rains begin. Moreover, the higher leaf N and P concentrations of the newly developed crown of deciduous species would potentially allow higher photosynthetic rates than evergreen trees at the end of the dry season and during the transition from the dry to the wet period.
Some shallow-rooted deciduous and evergreens are also able to flush before the onset of the rainy season (Table 1). In this case, a tight control of transpiration by reductions in stomatal conductance and partial or total leaf shedding coupled with osmotic adjustment and internal water storage may allow the plant to stabilize water balance (Bucci 2001; Eamus and Prior 2001). It has to be pointed out that only the upper soil layers are really depleted of water (Franco 2002). On the other hand, one could expect that the onset of leaf flush in these species would be greatly constrained in dry years and that small differences in root distribution may play a critical role.
In conclusion, Amass was significantly positively correlated with SLA, leaf N and P, which were higher in leaves of deciduous species. Thus, deciduous species compensated for their shorter leaf pay back period by maintaining higher potential payback capacity (higher values of maximum Amass) and lower leaf construction costs (higher SLA). The short leafless period of deciduous species may also partially offset the longer payback period of evergreen species, although it may involve the higher cost of maintaining a deep-root system or a tight control of plant water balance. On the other hand, evergreens may be greatly constrained in terms of producing leaves with a long life-span, because of accrued leaf damage by herbivores and pathogens and substantial reductions in stomatal conductance and partial leaf loss to stabilise water balance during the dry season.
References
Bataglia OC, Teixeira JPF, Furlani PR, Furlani AMC, Gallo JR (1978) Análise química de plantas. Circular no. 87. Secretaria de Agricultura, Coordenadoria de Pesquisa Agropecuária, Instituto Agronômico, São Paulo
Bucci SJ (2001) Arquitectura hidráulica y relaciones hídricas de árboles de sabanas neotropicales: efectos de la disponibilidad de agua y nutrientes. PhD thesis. Universidade de Buenos Aires, Buenos Aires
Bucci SJ, Goldstein G, Meinzer FR, Franco AC, Campanello P, Scholz FG (2004) Mechanisms contributing to seasonal homeostasis of minimum leaf water potential and predawn disequilibrium between soil and plant water potential in Neotropical savanna trees. Trees (in press)
Cardinot GK (1998) Efeitos de diferentes regimes de queimas nos padrões de rebrotamento de Kielmeyera coriacea Mart. e Roupala montana Aubl., duas espécies típicas do cerrado. Masters thesis. Universidade de Brasília, Brasília
Cordell S, Goldstein G, Meinzer FC, Vitousek PM (2001) Morphological and physiological adjustment to N and P fertilization in nutrient-limited Metrosideros polymorpha canopy trees in Hawaii. Tree Physiol 2:43–50
Duff GA, Myers BA, Williams RJ, Eamus D, O’Grady A, Fordyce IR (1997) Seasonal patterns in soil moisture, vapour pressure deficit, tree canopy cover and predawn water potential in a northern Australian savanna. Aust J Bot 45:211–224
Eamus D, Cole SC (1997) Diurnal and seasonal comparison of assimilation, phyllode conductance and water potential of three Acacia and one Eucalyptus species in the wet-tropics of Australia. Aust J Bot 45:275–290
Eamus D, Prior L (2001) Ecophysiology of trees of seasonally dry tropics: comparisons among phenologies. Adv Ecol Res 32:113–197
Ehleringer JR (1994) Variation in gas exchange characteristics among desert plants. In: Schulze E-D, Caldwell MM (eds) Ecophysiology of photosynthesis. Ecological studies series. Springer, Berlin Heidelberg New York, pp 361–392
Ehleringer JR, Cooper TA (1988) Correlations between carbon isotope ratio and microhabitat in desert plants. Oecologia 76:562–566
Eiten G (1972) The cerrado vegetation of Brazil. Bot Rev 38:201–341
Farquhar GD, Richards RA (1984) Isotopic composition of plant carbon correlates with water-use efficiency of wheat genotypes. Aust J Plant Physiol 11:539–552
Farquhar GD, O’Leary MH, Berry JA (1982) On the relationship between carbon isotope discrimination and the intercellular carbon dioxide concentration in leaves. Aust J Plant Physiol 9:121–137
Farquhar GD, Ehleringer JR, Hubick KT (1989) Carbon isotope discrimination during photosynthesis. Annu Rev Plant Physiol Mol Biol 40:503–537
Field C, Mooney H (1986) The photosynthesis–nitrogen relationship in wild plants. In: Givnish TJ (ed) On the economy of plant form and function. Cambridge University Press, Cambridge, pp 25–55
Franco AC (1998) Seasonal patterns of gas exchange, water relations and growth of Roupala montana, an evergreen savanna species. Plant Ecol 136:69–76
Franco AC (2002) Ecophysiology of cerrado woody plants. In: Oliveira OS, Marquis RJ (eds) The cerrados of Brazil: ecology and natural history of a neotropical savanna. Columbia University Press, New York, pp 178–197
Franco AC, Lüttge U (2002) Midday depression in savanna trees: coordinated adjustments in photochemical, efficiency, photorespiration, CO2 assimilation and water use efficiency. Oecologia 131:356–365
Givnish TJ (2002) Adaptive significance of evergreen vs. deciduous leaves: solving the triple paradox. Silva Fenn 36:703–743
Goldstein G, Rada F, Rundell P, Azocar A, Orozco A (1989) Gas exchange and water relations of evergreen and deciduous tropical savanna trees. Ann Sci For 46[Suppl]:448s–453s
Haridasan M (2000) Nutrição mineral de plantas nativas do cerrado. Rev Bras Fisiol Veg 12:54–64
Haridasan M (2001) Nutrient cycling as a function of landscape and biotic characteristics in the cerrados of Central Brazil. In: McClain ME, Victoria RL, Richey JE (eds) The biogeochemistry of the Amazon Basin. Oxford University Press, New York, pp 68–83
Jackson PC, Meinzer FC, Bustamante M, Goldstein G, Franco A, Rundel PW, Caldas L, Igler E, Causin F (1999) Partitioning of soil water among tree species in a Brazilian Cerrado ecosystem. Tree Physiol 19:717–724
Johnson C, Franco AC, Caldas LS (1983) Fotossíntese e resistência foliar em espécies nativas do cerrado: metodologia e resultados preliminares. Revta Brasil Bot 6:91–97
Lloyd J, Farquhar GD (1994) 13C discrimination during CO2 assimilation by the terrestrial biosphere. Oecologia 99:201–215
Maia JMF (1999) Variações sazonais das relações fotossintéticas, hídricas e crescimento de Caryocar brasiliense e Rapanea guianensis em um cerrado sensu stricto. Masters thesis. Universidade de Brasília, Brasília
Marquis RJ, Diniz IR, Morais HC (2001) Patterns and correlates of interspecific variation in foliar insect herbivory and pathogen attack in Brazilian cerrado. J Trop Ecol 17:1–23
Marquis RJ, Morais HC, Diniz IR (2002) Interactions among cerrado plants and their herbivores: unique or typical? In: Oliveira OS, Marquis RJ (eds) The cerrados of Brazil: ecology and natural history of a neotropical savanna. Columbia University Press, New York, pp 306–328
de Mattos EA, Reinert F, de Moraes JAPV (1997) Comparison of carbon isotope discrimination and CO2 and H2O exchange between the dry and the wet season in leaves of several cerrado woody species. Rev Bras Fisiol Veg 9:77–82
Medina E, Francisco M (1994) Photosynthesis and water relations of savanna tree species differing in leaf phenology. Tree Physiol 14:1367–1381
Meinzer G, Goldstein G, Grantz DA (1990) Carbon isotope discrimination in coffee genotypes grown under limited water supply. Plant Physiol 92:130–135
Meinzer FC, Goldstein G, Franco AC, Bustamante M, Igler E, Jackson P, Caldas L, Rundel PW (1999) Atmospheric and hydraulic limitations on transpiration in Brazilian cerrado woody species. Funct Ecol 13:273–282
Mooney HA, Gulmon SL (1982) Constraints on leaf structure and function in reference to herbivory. BioScience 32:198–206
Moraes JAPV, Prado CHBA (1998) Photosynthesis and water relations in cerrado vegetation. In: Scarano FR, Franco AC (eds) Ecophysiological strategies of xerophytic and amphibious plants in the Neotropics. (Oecologia Brasiliensis, vol 4.) PPGE-UFRJ, Rio de Janeiro, pp 45–63
Myers BA, Duff GA, Eamus D, Fordyce IR, O’Grady A, Williams RJ (1997) Seasonal variations in water relations of trees of differing leaf phenology in a wet–dry tropical savanna near Darwin, Northern Australia. Aust J Bot 45:225–240
Naves CC (2000) Relações hídricas e fotossíntese de duas espécies frutíferas do cerrado. Masters thesis. Universidade de Brasília, Brasília
Naves-Barbiero CC, Franco AC, Bucci SJ, Goldstein G (2000) Fluxo de seiva e condutância estomática de duas espécies lenhosas sempre-verdes no campo sujo e cerradão. Rev Bras Fisiol Veg 12:119–134
Prado CHBA, Moraes JAPV (1997) Photosynthetic capacity and specific leaf mass in twenty wood species of cerrado vegetation under field conditions. Photosynthetica 33:102–112
Ratter JA, Bridgewater S, Atkinson R, Ribeiro JF (1996) Analysis of the floristic composition of the Brazilian cerrado vegetation. II. Comparison of the woody vegetation of 98 areas. Edin J Bot 53:153–180
Reich PB, Schoettle AW (1988) Role of phosphorus and nitrogen on photosynthetic and whole plant carbon gain and nutrient use efficiency in Eastern white pine. Oecologia 77:25–33
Reich PB, Walters MB, Ellsworth DS (1992) Leaf life-span in relation to leaf, plant, and stand characteristics among diverse ecosystems. Ecol Monogr 62:365–392
Reich PB, Kloeppel BD, Ellsworth DS, Walters MB (1995) Different photosynthesis–nitrogen relations in deciduous hardwood and evergreen coniferous tree species. Oecologia 104:24–30
Reich PB, Walters MB, Ellsworth DS (1997) From tropics to tundra: global convergence in plant functioning. Proc Natl Acad Sci U S A 94:13730–13734
Reich PB, Ellsworth DS, Walters MB (1998) Leaf structure (specific leaf area) modulates photosynthesis–nitrogen relations: evidence from within and across species and functional groups. Funct Ecol 12:948–958
Reich PB, Ellsworth DS, Walters MB, Vose JM, Gresham C, Volin JC, Bowman WD (1999) Generality of leaf traits relationships: a test across six biomes. Ecology 80:1955–1969
Rivera G, Elliott S, Caldas LS, Nicolossi G, Coradin VTR, Borchert R (2002) Increasing day-length induces spring flushing of tropical dry forest trees in the absence of rain. Trees 16:445–456
Sobrado MA (1986) Aspects of tissue water relations and seasonal changes in leaf water potential components in evergreen and deciduous species coexisting in tropical dry forests. Oecologia 68:413–416
Sobrado MA (1991) Cost–benefit relationships in deciduous and evergreen leaves of tropical dry forest species. Funct Ecol 5:608–616
Sobrado MA (1994) Leaf age effect on photosynthetic rate, transpiration rate and nitrogen content in a tropical dry forest. Oecologia 96:19–23
Sobrado MA, Ehleringer JR (1997) Leaf carbon isotope ratios from a tropical dry forest in Venezuela. Flora 192:12–124
Sokal RR, Rohlf FJ (1995) Biometry: the principles and practice of statistics in biological research, 3rd edn. Freeman, New York
Acknowledgements
We thank the Reserva Ecológica do Instituto Brasileiro de Geografia e Estatística for logistic support and meteorological data. This investigation was supported by grants from the Conselho Nacional de Desenvolvimento Cientifico e Tecnológico and the PRONEX program, Brazil; the Interamerican Institute for Global Change Research; and a grant to A. C. Franco from the David Rockefeller Center for Latin American Studies at Harvard University
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Franco, A.C., Bustamante, M., Caldas, L.S. et al. Leaf functional traits of Neotropical savanna trees in relation to seasonal water deficit. Trees 19, 326–335 (2005). https://doi.org/10.1007/s00468-004-0394-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00468-004-0394-z