Abstract
Main conclusion
The structure of the cotton uceA1.7 promoter and its modules was analyzed; the potential of their key sequences has been confirmed in different tissues, proving to be a good candidate for the development of new biotechnological tools.
Abstract
Transcriptional promoters are among the primary genetic engineering elements used to control genes of interest (GOIs) associated with agronomic traits. Cotton uceA1.7 was previously characterized as a constitutive promoter with activity higher than that of the constitutive promoter from the Cauliflower mosaic virus (CaMV) 35S gene in various plant tissues. In this study, we generated Arabidopsis thaliana homozygous events stably overexpressing the gfp reporter gene driven by different modules of the uceA1.7 promoter. The expression level of the reporter gene in different plant tissues and the transcriptional stability of these modules was determined compared to its full-length promoter and the 35S promoter. The full-length uceA1.7 promoter exhibited higher activity in different plant tissues compared to the 35S promoter. Two modules of the promoter produced a low and unstable transcription level compared to the other promoters. The other two modules rich in cis-regulatory elements showed similar activity levels to full-length uceA1.7 and 35S promoters but were less stable. This result suggests the location of a minimal portion of the promoter that is required to initiate transcription properly (the core promoter). Additionally, the full-length uceA1.7 promoter containing the 5′-untranslated region (UTR) is essential for higher transcriptional stability in various plant tissues. These findings confirm the potential use of the full-length uceA1.7 promoter for the development of new biotechnological tools (NBTs) to achieve higher expression levels of GOIs in, for example, the root or flower bud for the efficient control of phytonematodes and pest-insects, respectively, in important crops.
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Introduction
Plants are constantly challenged by different and concomitant types of abiotic and biotic stresses (Crisp et al. 2016). Crops with new and superior characteristics are intensely demanded by agricultural producers and plant breeders worldwide to overcome these drawbacks (Hickey et al. 2019). Genetic engineering tools have contributed to the development of these elite cultivars and some of them have already been made commercially available (Basso et al. 2019). Several genes of interest (GOIs) have already been characterized in proof-of-concept studies and have been associated with desirable agronomic traits, such as water-deficit tolerance, salinity, heavy metals, flooding, or resistance to nematodes, pest-insects, bacteria, fungi, and viruses (Limera et al. 2017). For the development of new biotechnological tools (NBTs) using these GOIs, transcriptional promoter sequences that stably and adequately control the expression of these genes are highly important (Limera et al. 2017). Promoters that drive high levels of constitutive, stress-induced, organ-specific, or triggered expression in a particular plant growth phase may be required in NBTs to maintain or improve the GOI features or plant phenotype (Lu et al. 2008a).
The constitutive promoter from the Cauliflower mosaic virus (CaMV) 35S gene is currently the most commonly used promoter in plant engineering (Odell et al. 1985). Both the wild-type 35S promoter and its enhanced version can drive high transcription levels in dicotyledonous but show reduced and unstable activity in monocots (Benfey et al. 1990; Weeks et al. 1993; Gupta et al. 2001). In contrast, monocot-derived promoters usually have their activity potentiated only in monocot plants, whereas dicot-derived promoters have lower activity in monocots (Park et al. 2010). Ubiquitin-promoter sequences are efficient to drive constitutively high expression levels of transgenes in both monocot and dicot plants. The Ubi1 from maize (Christensen et al. 1992), Ubi1 and 3 from rice (Wang and Oard 2003; Lu et al. 2008b), Ubq10 from A. thaliana (Grefen et al. 2010), uceA1.7 from cotton (Viana et al. 2011), Ubi4 and 9 from sugarcane (Wei et al. 2003), GmUbi from soybean (Hernandez-Garcia et al. 2009), and Ubi7 from potato (Garbarino et al. 1995) are some species-specific examples. Although there are several plant and virus promoters functionally characterized as constitutive, few of these promoters are validated in other crops or characterized for the transcription level in different tissues or stages of development of the transgenic plant. Additionally, gene stacking in a single transgenic plant requires a greater number of different promoters with high activity to avoid homology-dependent gene silencing when using multiple copies of the same promoter (Park et al. 2010). Furthermore, promoters with higher activity than those already available are required to obtain a greater accumulation of transcripts or proteins in specific plant organs (e.g., entomotoxic protein in cotton flower bud to control the cotton boll weevil) (Ribeiro et al. 2017).
Plant ubiquitin-conjugating (E2) enzymes have a conserved ubiquitin-conjugating domain responsible for modulating the post-transcriptional degradation of target proteins (Moon et al. 2004). In addition, E2 enzymes are also essential for plant immunity (Zhou et al. 2017). The E2 enzyme-related coding gene (Gohir.A11G023700) from cotton (Gossypium hirsutum) is highly expressed in different organs and tissues. The promoter sequence of this gene (named uceA1.7) was isolated and characterized as a constitutive promoter with activity higher than CaMV 35S in different tissues of transgenic Arabidopsis thaliana, such as root, stem, leaf, and flower bud (Viana et al. 2011). In addition, the long 5′-UTR of the gene, which contains an intron sequence, was considered essential to the high expression level of the gus reporter gene (β-glucuronidase). The uceA1.7 promoter has been patented (US8227588B2) and recently used to control Cry10Aa gene expression in the development of transgenic cotton resistant to cotton boll weevil (Anthonomus grandis) (Ribeiro et al. 2017).
In this study, we generated A. thaliana homozygous events stably overexpressing the gfp (green fluorescent protein) reporter gene driven by the full-length and four different modules of the uceA1.7 promoter and compared the transcriptional level and stability with the 35S promoter. The module 1 of the uceA1.7 promoter containing the core promoter and 5′-UTR, module 2 without the 5′-UTR, module 3 without the 5′-UTR and core promoter identified in this study, and module 4 containing only the core promoter sequence predicted previously by Viana et al. (2011) were evaluated. Our findings suggest the minimal promoter sequence, proper location of the core promoter, and reinforce that full-length uceA1.7 promoter containing the 5′-UTR is essential to drive higher transcriptional stability in different plant tissues.
Materials and methods
In silico analysis of the promoter and adjacent sequences
The genomic sequences corresponding to uceA1.7 (position A11:2079996..2084242; gene Gohir.A11G023700) were retrieved from Gossypium hirsutum v1.1 (Zhang et al. 2015) by the Phytozome v.12 database (Goodstein et al. 2012). Additional sequences, such as constitutive promoters from CaMV 35S (Odell et al. 1985; Somssich 2019), Cestrum yellow leaf curling virus (CmYLCV) (Sanger et al. 1990; Stavolone et al. 2003), Soybean chlorotic mottle virus (pIV_SoyCMV and pNCR_SoyCMV) (Hasegawa et al. 1989; Conci et al. 1993; Fukuoka et al. 2000), Figwort mosaic virus (FMV) (Sanger et al. 1990), Sugarcane bacilliform virus (SBCV) (Tzafrir et al. 1998), Banana streak virus (BSV) (Harper and Hull 1998), FMV full-length transcript (FLt) (Maiti et al. 1997), and the promoters flower-specific 1 (FS1) from cotton (Artico et al. 2014), petal-specific anthocyanidin synthase 1 (NtANS1) from Nicotiana tabacum (Lim et al. 2013) were also included in cis-regulatory elements analysis. Initially, some cis-acting regulatory DNA elements were identified using PlantCARE (Lescot et al. 2002), PLACE (Higo et al. 1999), and Plant-PAN 3.0 (Chang et al. 2008; Chow et al. 2019) online database with default parameters. All three databases are public and online resources of cis-regulatory elements identified in monocot and dicot plants, which are updated periodically. The Z score index (Ma et al. 2013) was also used to identify the cis-regulatory elements with a Z score ≥ 5, while their descriptions were retrieved from the PLACE database (Higo et al. 1999) (https://www.dna.affrc.go.jp/PLACE/place_seq.shtml). Based on the cis-regulatory elements identified in this study and previously by Viana et al. (2011), four modules for the uceA1.7 promoter were planned (Fig. 1).
Agrobacterium-mediated plant transformation
Binary vectors were synthesized by the company Epoch Life Science (Sugar Land, TX, EUA) and subsequently transfected into the A. tumefaciens strain GV3101. The gfp reporter gene was cloned under control of the full-length uceA1.7, CaMV 35S enhanced, and the four modules of the uceA1.7 promoter (Fig. 1b). Hygromycin phosphotransferase II (hptII) was used as a selection marker gene under control of the ubiquitin-ribosomal protein (ubi3) promoter from tomato (Fig. 1b). A. thaliana ecotype Col-0 was genetically transformed by the floral dip method (Clough and Bent 1998) and selected in vitro using 25 mg L−1 hygromycin B (Invitrogen) as described by Harrison et al. (2006). Resistant plants were screened by conventional PCR using specific primers (Suppl. Table S1). The generations (T1–T4) were advanced with independent events and six homozygous T4 events were chosen for the molecular characterization of the expression profile of the reporter gene in different plant tissues.
Tissue-specific expression level in transgenic events
Seeds from wild-type and transgenic A. thaliana lines were surface sterilized with 75% ethanol for 1 min, sterilized again with 2% sodium hypochlorite solution plus Tween-20 for 10 min, and subsequently rinsed six times with sterile water. Seeds were placed on plates containing half-strength MS medium (Murashige and Skoog 1962) supplemented with 0.8% agar and maintained in the dark for 3 days at 4 °C for stratification. Plants were maintained in a climate-controlled growth room at 22 ± 2 °C, 70–80% relative humidity, with the light intensity 100 μmol photons m−2 s−1, and 16/8-h light/dark photoperiod. Root, stem, leaf, silique, and flower bud tissues were harvested, ground in liquid nitrogen using mortar and pestle, and stored at − 80 °C. The total RNA was isolated using TRIzol Reagent (Invitrogen) according to the manufacturer’s instructions. The RNA concentration was estimated using a NanoDrop 2000 spectrophotometer (Thermo Scientific), and its integrity was evaluated with 1% agarose gel electrophoresis. RNA samples were treated with RNase-free RQ1 DNase I (Promega) according to the manufacturer’s instructions. Then 2 μg of DNase-treated RNA was used as a template for cDNA synthesis using Oligo-(dT)20 primer and SuperScript III RT (Life Technologies), according to the manufacturer’s instructions. The cDNA was quantified by spectrophotometry and diluted with nuclease-free water to 400 ng µL−1. RT-qPCR assays were performed in an Applied Biosystems 7500 Fast Real-Time PCR System (Applied Biosystems) using 400 ng of cDNA, 0.2 µM of each gene-specific primer (Suppl. Table S1) and GoTaq® qPCR Master Mix (Promega). Conditions for RT-qPCR included an initial 95 °C for 10 min, then 40 cycles of 95 °C for 15 s and 60 °C for 1 min, followed by melting curve analysis. The expression level was normalized using the Actin 2 endogenous reference gene. In a preliminary RT-qPCR assay using three reference genes (GAPDH, EF1, and Actin 2) and some cDNA samples, the Actin 2 reference gene showed the highest stability (Suppl. Table S1). Three biological replicates for each independent event and four plants for each biological replicate were used, while all samples were carried out in technical triplicate reactions. Primer efficiencies and target-specific amplification were confirmed by a single and distinct peak in melting curve analysis. The relative expression level was calculated using the delta–delta cycle threshold (∆∆CT) method (Schmittgen and Livak 2008).
Transcriptional stability over generations
The transcriptional stability of the gfp reporter gene driven by full-length uceA1.7, module 1, module 2, and 35S promoters was evaluated over the T5 and T6 generations from A. thaliana homozygous events. Three independent events from each promoter were selected, germinated in vitro, and grown in the growth chamber. Leaf, flower bud, and root tissues were harvested from adult plants, RNA was isolated, and cDNA was synthesized as described above. The expression profile was evaluated using real-time RT-qPCR, normalized with the Actin 2 reference gene, and represented as fold-change values calculated using the delta–delta cycle threshold (∆∆CT) method (Schmittgen and Livak 2008). Three biological replicates for each independent event and four plants for each biological replicate were used, while all biological replicates were carried out in technical triplicate reactions.
Results
In silico analysis of the promoter and adjacent sequences
The TATA-box, YR Rule, TSS, some other cis-regulatory elements, and a long 5′-UTR containing an intron sequence of the cotton uceA1.7 promoter were previously predicted by Viana et al. (2011). In this study, using in silico analysis, it was verified that this long 5′-UTR contains the core promoter and a short 5′-UTR with an intron sequence, both of which are rich in cis-regulatory elements (Suppl. File S1). Using the PlantCARE, Plant-PAN, and PLACE databases, several cis-regulatory elements were identified in the upstream region (843 bp length) of the uceA1.7 promoter, in the full-length uceA1.7 promoter sequence, in its 5′-UTR sequence, in its primary transcript (exon and intron sequences), and in its 3′-UTR and additional (378-bp length) downstream sequences (Suppl. Table S2). In the full-length uceA1.7 promoter sequence, 542 cis-regulatory elements were identified, while in its short 5′-UTR, 209 cis-regulatory elements were identified, compared to 428 cis-regulatory elements identified in the 35S promoter. In contrast, using a Z score index supported by high statistical significance (P value less than 0.05), 17 cis-regulatory elements (8 unique) were identified in the full-length uceA1.7 promoter sequence and 4 cis-regulatory elements (2 unique) in its 5′-UTR, while in the 35S promoter, 47 cis-regulatory elements (14 unique) were identified (Suppl. Tables S3, S4). A high number of cis-regulatory elements, 1083 and 326, respectively, were also identified in the uceA1.7 transcript sequence and in its 3′-UTR using the online databases (Suppl. Table S2). Similarly, using the Z score index 143 (40 unique) and 3 cis-regulatory elements were identified in the transcript sequence and its 3′-UTR, respectively (Table 2). The common cis-acting element in promoter and enhancer regions (PlantCARE), binding site for bZIP transcription factors (TFs), binding site for DOF zinc finger protein (Plant-PAN), core site required for binding of DOF proteins and tetranucleotide of mesophyll expression module 1 (PLACE) were the most represented in the full-length uceA1.7 promoter. In contrast, the binding sites for bZIP, MYB, AP2, and bHLH TFs were predominant in the 35S promoter (Suppl. Table S2). In comparison with other viral constitutive or tissue-specific promoters, using the Z score index, it was observed that full-length uceA1.7 promoter has a similar amount of cis-regulatory elements (8 unique or 17 redundant), but with slightly different characteristics (Suppl. Table S2). The following number of cis-regulatory elements were identified in other promoters as CmYLCV (eight unique or nine redundant), pIV_SoyCMV (eight unique or eight redundant), pNCR_SoyCMV (three unique or four redundant), FMV (11 unique or 13 redundant), SCBV (20 unique or 50 redundant), BSV (15 unique or 18 redundant), FLt (12 unique or 30 redundant), cotton flower-specific FS1 (seven unique or 11 redundant), and tobacco petal-specific NtANS1 (14 unique or 37 redundant). The most representative cis-regulatory elements of the uceA1.7 promoter sequence were the binding sites to TFs involved in hormonal signaling, while in the viral promoters analyzed were the binding sites to TFs involved in the plant defense response.
From this previous analysis of cis-regulatory elements and core promoters, four modules from the full-length uceA1.7 promoter were analyzed (Fig. 1a; Suppl. Fig. S1a to S1d; Suppl. File S2). In the modules 1 to 4, 395, 448, 343, and 169, cis-regulatory elements were identified based on three online databases (Suppl. Table S2). In contrast, using the Z score index, 13 (six unique) cis-regulatory elements were identified in modules 1 and 2, while in modules 3 and 4, only one and no cis-regulatory elements were identified, respectively (Suppl. Tables S3, S4). The minimal promoter sequence, the core promoter, the contribution of 5′-UTR in enhancing and stability of the transcription, putative uceA1.7 promoter module with higher tissue-specific activity were evaluated. Module 1 containing the core promoter and 5′-UTR, module 2 without the 5′-UTR, module 3 without the 5′-UTR and core promoter identified in this study, and module 4 containing only the core promoter sequence predicted previously by Viana et al. (2011) were synthesized and cloned into binary vectors to drive expression of the gfp reporter gene.
Plant transformation
Around 15 independent events of A. thaliana from each module were obtained overexpressing the gfp reporter gene driven by the full-length uceA1.7 promoter, its four modules, and the 35S promoter (Fig. 1b; Suppl. Fig. S1a). Transgenic plants were screened in MS medium containing hygromycin B and confirmed by conventional PCR (Suppl. Fig. S2a, b). Plant generations were advanced to achieve homozygous events. Six homozygous events from each promoter were selected for molecular characterization and the clone plants from each event were propagated in a growth chamber.
Tissue-specific expression level
Root, stem, leaf, silique, and flower buds were harvested from adult plants, and the expression profile of the gfp reporter gene was monitored using real-time RT-qPCR (Fig. 2a). The full-length uceA1.7 promoter showed higher activity in the root and flower buds than did the 35S promoter, while in the stem, leaf, and silique tissues, no differences were found (Fig. 2b). In contrast, module 4 presented the lowest activity among the four evaluated modules in both plant tissues evaluated, while modules 1 and 2 had the highest activity. However, both modules showed lower activity than the full-length uceA1.7 and 35S promoters in almost all tissues evaluated.
Transcriptional stability over generations
Three T5 and T6 independent events (homozygous) from module 1, module 2, full-length uceA1.7, and 35S promoter were evaluated with respect to transcriptional stability over these two generations conferred by the presence of 5′-UTR. Leaf, flower bud, and root tissues were harvested from adult plants and screened using real-time RT-qPCR (Fig. 3a–c). Module 1 showed greater transcriptional stability, similar to the full-length uceA1.7 promoter, while module 2 was more unstable over these two generations.
Discussion
Time-, tissue- and amount-specific transcriptional gene regulations play an important role in all biological processes and in plant development. These fine-tuning mechanisms are controlled mainly by interactions between promoter sequences and the numerous TFs (Liu and Stewart 2016). In addition, other proteins (co-activators) also interact with the operon sequences and act to modulate the transcriptional level (initiation, repression, or regulation of transcription). Typical encoding-protein promoters often contain the TATA-box domain located ~ 30–100 bp upstream of the transcription start site (TSS), which is considered essential to transcriptional initiation (Burley and Roeder 1996; Smale and Kadonaga 2003). Other motifs may also be present in this promoter region near the TATA-box (e.g., YR Rule, Y-Patch, and REGs) (Yamamoto et al. 2007). The region containing these motifs and the binding sites for the basic transcriptional machinery (e.g., RNA polymerase II and its subunits) is denominated the core promoter (Molina and Grotewold 2005). Proximal and distal regions of the core promoter contain several enhancers, repressors, insulators, and cis-regulatory element sequences that contribute to the regulation of gene expression (Bulger and Groudine 2011). These cis-regulatory elements are binding sites to TFs, and the amount, their features, and spacing between them define the temporal and spatial expression levels. In addition, these cis-regulatory elements are also found in the 5′-UTR, exons, introns, and 3′-UTR conferring more transcriptional stability of the gene or even acting in the initiation of transcription (Hernandez-Garcia and Finer 2014; Biłas et al. 2016). This genome region upstream of the gene containing all these transcriptional regulatory elements, which may or may not include the 5′-UTR, is named the minimal promoter sequence (or full-length promoter) and considered essential for correct transcription (local, temporal, and amount specific) of the target gene. In some cases, partial sequences from the full-length promoter (named promoter modules) containing specific cis-regulatory elements may confer greater constitutive, plant tissue- or stage-specific transcriptional activity (Wang et al. 2015). Smaller (compact) promoter sequences that have a high transcriptional activity that is constitutive, induced, tissue- or developmental stage-specific are of extreme relevance for the development of NBTs (Limera et al. 2017; Basso et al. 2019). Promoters with high activity in flower buds, roots and leaves are required for efficient control of pests that specifically attack these tissues, for example, cotton boll weevil, nematodes, and caterpillars in important crops (e.g., cotton, soybean, maize, and sugarcane) (Ribeiro et al. 2017; Wang et al. 2017).
The uceA1.7 promoter was isolated from cotton (Viana et al. 2011) and used to drive the stable expression of the Cry10Aa entomotoxic protein against the cotton boll weevil (Anthonomus grandis) (Ribeiro et al. 2017). Viana et al. (2011) verified that this promoter had a constitutive and higher activity than the viral 35S promoter, in different tissues of transgenic A. thaliana. Ribeiro et al. (2017) confirmed that this promoter has constitutive and high activity in cotton flower buds. In this work, we generated four modules from this promoter to identify its core promoter region, minimal promoter sequence, the relevance of its 5′-UTR, and the constitutive and tissue-specific expression levels in A. thaliana. Viana et al. (2011) identified by in silico prediction a TATA-box and the putative TSS sequence in the uceA1.7 promoter, highlighting the importance of the long 5′-UTR for the high expression of the gus reporter gene in A. thaliana events. However, supported by the recent cotton genome and transcriptome sequencing data available from the Phytozome database (Goodstein et al. 2012; Zhang et al. 2015), new TATA-box and TSS for the uceA1.7 promoter are suggested in this work. Consequently, the long 5′-UTR predicted initially corresponds, in fact, to the core promoter and a short 5′-UTR rich in cis-regulatory elements. In addition, the distribution of cis-regulatory elements associated with the transcriptional level conferred by each module of this promoter indicated the minimal promoter sequence and strong importance to the 5′-UTR for high expression level and stability. Curiously, overexpression of GOIs driven by the full-length uceA1.7 promoter in transgenic cotton showed high transcriptional stability in different plant tissues, development stages, and in flower buds of different sizes (unpublished data). Similar results were obtained using modules from the 35S promoter, indicating that the deletion of its core promoter strongly decreases transcriptional activity (Benfey and Chua 1990). In addition, we identified a transcriptional enhancer sequence downstream of the core promoter, and verified that tissue-specific gene expression depends on synergistic interactions among cis-regulatory elements. Our data also confirm that the full-length uceA1.7 promoter has higher activity in root and flower bud tissues compared to the 35S promoter, whereas it was equivalent in the other tissues (stem, leaf, and silique), supporting its use in the development of NBTs focused on these plant tissues.
Some promoters of viral origin have been successfully used in plant genetic engineering, primarily because they are compact sequences, rich in cis-regulatory elements, and already validated in several plant species (Acharya et al. 2014). However, at present, the level of target gene expression conferred by these promoters may not yet be high enough in the desired tissue (Artico et al. 2014; Lambret-Frotte et al. 2016; Ribeiro et al. 2017). Additionally, new promoters with high tissue-specific activity are currently demanded for the gene stacking associated with multiple agronomic traits in several crops (Dong and Ronald 2019). In silico analysis of cis-regulatory elements of the uceA1.7 promoter compared to flower-specific (NtANS1 and FS1) and viral promoters (35S, CmYLCV, SoyCMV, FMV, SBCV, and BSV) shows that these elements are not fully conserved and clearly related to tissue-specific or constitutive activity of the promoter. Thus, the overall contribution (and not specific or only specific) of these cis-regulatory elements may be more related to the typical regulation of gene expression (Hernandez-Garcia and Finer 2014). The use of synthetic promoters that combine classical promoters with enhancer sequences, partial sequences from tissue-specific promoters, regions rich in cis-regulatory elements from other promoters, introns, 5′-UTR, and double 3′-UTR is an interesting alternative that has shown promising results (van der Meer et al. 1990, 1992; Wang et al. 2015; Liu and Stewart 2016; Diamos and Mason 2018). Therefore, the use of the full-length uceA1.7 promoter in combination with enhancer sequences may lead to an increased tissue-specific expression. Since the cotton crop is the target of numerous insect pests and nematodes, the use of this promoter in the original culture for the development of NBTs to overcome these drawbacks is an additional possibility.
Conclusions
In the present study, we further characterized the cotton uceA1.7 promoter at both the structural level and the ability to control the tissue-specific expression of GOIs. Our data provided evidence of the correct location of a minimal portion of the uceA1.7 promoter required to initiate transcription properly (core promoter). The full-length uceA1.7 promoter containing the 5′-UTR was considered to be essential for higher transcriptional stability in different plant tissues. These findings highlighted the potential use of the full-length uceA1.7 promoter for the development of NBTs, mainly for cotton crops.
Author contribution statement
MFGS was the lead researcher for all the work and provided intellectual input and financial support. ITL-T planned the four uceA1.7 modules and designed the binary vectors. MFB performed the plant transformation, the advancement of generations and, helped by ITL-T, performed the real-time RT-qPCR assays. EOF performed the CaMV 35: green fluorescent protein (GFP) plant transformation and the advancement of generations. CEMP and TPR helped with the screening of plants and generation advancement. MFB, CB, and ACO performed all analyses of cis-regulatory elements from promoter sequences. MFB wrote the manuscript, while JAE, MAF and MFGS provided intellectual input. All authors read and approved the final version.
Abbreviations
- GOIs:
-
Genes of interest
- NBTs:
-
New biotechnological tools
- CaMV:
-
Cauliflower mosaic virus
- UTR:
-
Untranslated region
- TSS:
-
Transcriptional start site
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Acknowledgements
We are grateful to EMBRAPA, CAPES, CNPq, INCT PlantStress Biotech, and FAP-DF for providing financial support for this scientific research.
Funding
MFB is grateful to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for a post-doctoral research fellowship (PDJ 150936/2018-4). This work was supported by grants from CAPES, CNPq, FAP-DF, INCT, and EMBRAPA.
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425_2020_3348_MOESM1_ESM.tif
Supplementary file1 (TIF 6714 kb) Overview of the four uceA1.7 modules (highlighted in bold background) aligned with the full-length uceA1.7 promoter. a Module 1 containing 681 bp. b Module 2 containing 792 bp. c Module 3 containing 581 bp. d Module 4 containing 251 bp were planned around the core promoter elements predicted in this study or by Viana et al. (2011). ORF, open reading frame; SC, translation start codon; TSS, transcription start site; 5’ UTR, 5’ untranslated region
425_2020_3348_MOESM2_ESM.tif
Supplementary file2 (TIF 9023 kb) Genetic transformation of Arabidopsis thaliana mediated by Agrobacterium tumefaciens GV3101 strain. a Six independent events from each full-length promoter (uceA1.7 and CaMV 35S) or four uceA1.7 modules (Mod 1 to 4) were evaluated. b PCR detection of the transgene in A. thaliana T1 events using specific primers for the gfp (green fluorescent protein) reporter gene (Suppl. Table 1). Marker: 1.0-kb DNA ladder (Invitrogen® Cat. #10787018); WT, wild-type A. thaliana line used as a negative control for PCR and RT-qPCR assays; C+, DNA plasmid used as a positive control for the PCR assay
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Basso, M.F., Lourenço-Tessutti, I.T., Busanello, C. et al. Insights obtained using different modules of the cotton uceA1.7 promoter. Planta 251, 56 (2020). https://doi.org/10.1007/s00425-020-03348-8
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DOI: https://doi.org/10.1007/s00425-020-03348-8