Abstract.
Treatment with hyperbaric O2 (HBO) ameliorates ischemia-reperfusion (I/R) injury. Since tumor necrosis factor-α (TNF-α) plays an important role in I/R injury, we hypothesized that the effect of HBO in I/R injury may be due to its ability to inhibit TNF-α production. In this study, one group of rats received HBO during 60 min of ischemia (HBO group, n=9), while control rats endured the same procedure but did not receive HBO (non-HBO, n=9). A group of sham-operated control rats (SHAM, n=6) underwent laparotomy without occlusion of the artery and HBO treatment. Intestinal I/R led to an increase in serum TNF-α concentration to [mean (SEM)] 165 (32) pg/ml (P<0.01 vs SHAM rats). HBO attenuated this increase [34 (9) pg/ml; P<0.05 vs non-HBO group]. Intestinal I/R also resulted in a marked increase in lung myeloperoxidase content [0.62 (0.04) U/g vs 0.17 (0.02) U/g of SHAM rats, P<0.01]. HBO suppressed this increase [0.40 (0.04) U/g, P<0.05 vs non-HBO rats]. HBO ameliorated the injury to the intestine and lung. The number of neutrophils sequestered in the lung was reduced in HBO rats compared to non-HBO rats [6.4 (0.9) neutrophils/per oil field and 10.9 (2) neutrophils/per oil field, respectively; P<0.05]. These findings demonstrate that HBO inhibits TNF-α production during intestinal I/R, and this reduced TNF-α production may be attributed to the beneficial effects of HBO.
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Yang, Zj., Bosco, G., Montante, A. et al. Hyperbaric O2 reduces intestinal ischemia-reperfusion-induced TNF-α production and lung neutrophil sequestration. Eur J Appl Physiol 85, 96–103 (2001). https://doi.org/10.1007/s004210100391
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DOI: https://doi.org/10.1007/s004210100391