Abstract
An in vitro method for obtaining plants of Acacia catechu has been developed using nodal explants from mature `elite' trees growing in the field. Maximum shoot bud development (eight to ten) from a single explant was achieved on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) (4.0 mg/l) and α-naphthaleneacetic acid (0.5 mg/l). Addition of adenine sulphate (25.0 mg/l), ascorbic acid (20.0 mg/l) and glutamine (150.0 mg/l) to the medium was found beneficial for maximum shoot bud induction. The shoot buds developed into healthy and sturdy shoots on MS medium containing BAP and kinetin at 1.0 mg/l. Excised shoots were rooted on 1/4-strength MS medium with indole-3-acetic acid at 3.0 mg/l and 1.5% sucrose to obtain complete plants.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Additional information
Received: 17 June 1997 / Revision received: 11 September 1997 / Accepted: 27 September 1997
Rights and permissions
About this article
Cite this article
Kaur, K., Verma, B. & Kant, U. Plants obtained from the Khair tree (Acacia catechu Willd.) using mature nodal segments. Plant Cell Reports 17, 427–429 (1998). https://doi.org/10.1007/s002990050419
Issue Date:
DOI: https://doi.org/10.1007/s002990050419