Abstract
By using highly regenerative calluses, we developed a stable transformation system in garlic (Allium sativum L.). The temperature and number of days of co-cultivation with Agrobacterium tumefaciens was shown to be an important factor in transient expression of the uid A gene. After a culture period of 5 months in selection medium containing hygromycin, 20 shoots were induced from ca. 1000 calluses, among which 15 plants expressed β-glucuronidase activity upon staining with X-Gluc. Shoots developed into transgenic garlic after 1 month. Integration of the uid A gene was confirmed by Southern blot analysis for genomic DNA of transgenic garlic plants.
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Received: 25 October 1999 / Revision received: 16 February 2000 / Accepted: 22 February 2000
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Kondo, T., Hasegawa, H. & Suzuki, M. Transformation and regeneration of garlic (Allium sativum L.) by Agrobacterium-mediated gene transfer. Plant Cell Reports 19, 989–993 (2000). https://doi.org/10.1007/s002990000222
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DOI: https://doi.org/10.1007/s002990000222