Abstract
The continuous fermentation of 1,3-propanediol from glycerol by Clostridium butyricum was subjected to cell recycling by filtration using hollow-fibre modules made from polysulphone. The performance of the culture system was checked at a retention ratio (dilution rate/bleed rate) of 5, dilution rates between 0.2 h−1 and 1.0 h−1 and glycerol input concentrations of 32 g l−1 and 56 g l−1. The near-to-optimum propanediol concentration of 26.5 g l−1 (for 56 g l−1 glycerol) was maintained up to a dilution rate of 0.5 h−1 and then decreased while the propanediol productivity was highest at 0.7 h−1. The productivity could be increased by a factor of four in comparison to the continuous culture without cell recycling. By application of the model of Zeng and Deckwer [(1995) Biotechnol Prog 11: 71–79] for cultures under substrate excess, it was shown that the limitations resulted exclusively from product inhibition and detrimental influences from the cell recycling system, such as shear stress, were not involved.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Additional information
Received: 20 October 1997 / Received revision: 12 December 1997 / Accepted: 14 December 1997
Rights and permissions
About this article
Cite this article
Reimann, A., Biebl, H. & Deckwer, WD. Production of 1,3-propanediol by Clostridium butyricum in continuous culture with cell recycling. Appl Microbiol Biotechnol 49, 359–363 (1998). https://doi.org/10.1007/s002530051182
Issue Date:
DOI: https://doi.org/10.1007/s002530051182