Abstract
Benzo[e]pyrene is a pentacyclic aromatic hydrocarbon, which, unlike its structural isomer benzo[a]pyrene, is not a potent carcinogen or mutagen. The metabolism of benzo[e]pyrene was studied using the filamentous fungus Cunninghamella elegans ATCC 36112. C. elegans metabolized 65% of the [9, 10, 11, 12-3H]benzo[e]pyrene and unlabeled benzo[e]pyrene added to Sabouraud dextrose broth cultures after 120 h of incubation. Three major metabolites of benzo[e]pyrene were separated by reversed-phase high-performance liquid chromatography. These metabolites were identified by 1H and 13C NMR, UV-visible, and mass spectral analyses as 3-benzo[e]pyrenylsulfate, 10-hydroxy-3-benzo[e]pyrenyl sulfate, and benzo[e]pyrene 3-O-β-glucopyranoside.
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Received: 7 September 1995/Received revision: 14 November 1995/Accepted: 11 December 1995
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Pothuluri, J., Evans, F., Heinze, T. et al. Formation of sulfate and glucoside conjugates of benzo[e]pyrene by Cunninghamella elegans . Appl Microbiol Biotechnol 45, 677–683 (1996). https://doi.org/10.1007/s002530050747
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DOI: https://doi.org/10.1007/s002530050747