Abstract
Deep-sea corals provide important habitat for many organisms; however, the extent to which fishes and other invertebrates are affiliated with corals or other physical variables is uncertain. The Cape Fear coral mound off North Carolina, USA (366–463 m depth, 33° 34.4′N, 76° 27.8′W) was surveyed using multibeam sonar and the Johnson-Sea-Link submersible. Multibeam bathymetric data (2006) were coupled with in situ video data (2002–2005) to define habitat associations of 14 dominant megafauna at two spatial scales. Results suggested greater habitat specificity of deep-reef fauna than previously documented, with fishes showing greater affinity for certain habitat characteristics than most invertebrates. High vertical profile, degree of coral coverage, and topographic complexity influenced distributions of several species, including Beryx decadactylus, Conger oceanicus, and Novodinia antillensis on the smaller scale (30 × 30 m). On the broad scale (170 × 170 m), several suspension feeders (e.g., N. antillensis, anemones), detritivores (Echinus spp.), and mesopelagic feeders (e.g., Beryx decadactylus, Eumunida picta) were most often found on the south-southwest facing slope near the top of the mound. Transient reef species, including Laemonema barbatulum and Helicolenus dactylopterus, had limited affiliations to topographic complexity and were most often on the mound slope and base. Megafauna at deep-water reefs behave much like shallow-water reef fauna, with some species strongly associated with certain fine-scale habitat attributes, whereas other species are habitat generalists. Documenting the degree of habitat specialization is important for understanding habitat functionality, predicting faunal distributions, and assessing the impacts of disturbance on deep-reef megafauna.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Marine species are often predictably affiliated with certain habitats due to a combination of ecological, biological, and environmental factors operating over various temporal and spatial scales. Species-habitat associations are well-studied in shallow-water (<200 m) ecosystems, including seagrass beds (e.g., Orth et al. 1984; Attrill et al. 2000), coral reefs (e.g., Hixon and Beets 1993; Munday et al. 1997), and Sargassum spp. mats (e.g., Casazza and Ross 2008). Species-specific assemblages have also been documented using submersibles and ROVs in structurally complex habitats (e.g., deep-sea coral reefs, tubeworm aggregations, and mussel beds) along continental margins (200–4,000 m; see Levin et al. 2010). However, faunal associations with fine-scale habitat parameters (e.g., vertical profile, topographic complexity) that are often affiliated with shallow-water reef fauna are less well-known in the deep sea (>200 m). Detailed, topographic data produced by multibeam sonar have facilitated species-habitat association studies, and integrating these data with in situ video provides the opportunity to examine and map the distribution of fauna relative to habitat variables in deep-sea ecosystems (Wilson et al. 2007).
Biogenic habitats, such as deep-sea (cold-water) coral reefs, like shallow-water reefs, concentrate resources and increase local diversity (e.g., Jensen and Frederiksen 1992; Ross and Quattrini 2007). In the deep Atlantic Ocean, Lophelia pertusa is the dominant reef-building coral (Roberts et al. 2009), but other scleractinians, antipatharians, octocorals, and sponges attach to existing hardbottoms (e.g., Roberts et al. 2006, 2009) and form deep-reef habitat. Deep-water reefs harbor higher species richness, higher densities of organisms, and different species assemblages compared to surrounding non-reef habitats (e.g., Mortensen et al. 1995; Ross and Quattrini 2007), but the degree to which fauna are closely associated with deep-reef habitat varies by location (Ross and Quattrini 2009; Buhl-Mortensen et al. 2010). Despite increasing studies on the biodiversity of deep-sea reefs (e.g., Cordes et al. 2008; Roberts et al. 2008), little quantitative data exist on how habitat structure, particularly at fine spatial scales, influences the associated megafaunal communities (Roberts et al. 2008). The question remains whether deep-reef megafauna exhibit similar habitat-specific associations to species inhabiting shallow-water coral reefs.
Broadly defined habitat type (i.e., coral, cobble, and rubble) and depth are two important factors influencing abundance, diversity, and distribution of megafaunal species at deep-sea reefs. For example, species richness and abundance of sessile and mobile invertebrates were often comparable between L. pertusa and other structured habitats, such as coral rubble or rock, yet differed from mud, sand, and cobbles (Mortensen et al. 1995; Roberts et al. 2008). In addition, different substrates (outcrop, gravel, and boulders) with varying degrees of attached fauna influenced the distribution of the fish assemblages in the Gulf of Maine (Auster 2005). In contrast, depth, not habitat type, influenced the species composition of deep-reef fish assemblages in the northeastern Atlantic (Costello et al. 2005). Both differences in habitat structure and depth likely influenced the distribution of deep-reef fish assemblages of the southeastern US (SEUS; North Carolina through Florida; Ross and Quattrini 2007, 2009). Although these studies provided insight into broad scale distribution patterns at deep-sea reefs and nearby habitats, knowledge concerning fine-scale habitat affiliations is still lacking.
A characteristic fauna (particularly fishes) associated with deep-water reefs appears to distinguish SEUS and Gulf of Mexico (GOM) deep reefs from those in other regions (Ross and Quattrini 2007; Sulak et al. 2007). This unique fauna includes recently discovered species (McCosker and Ross 2007; Fernholm and Quattrini 2008; Nielsen et al. 2009; Mah et al. 2010; Anker and Nizinski 2011) and numerous species not previously known in those regions (Caruso et al. 2007; Ross and Quattrini 2007; Henry et al. 2008). This suggests that megafauna associated with deep reefs may have stronger affiliations with particular habitats within the SEUS compared to other deep reefs, such as those in more northern Atlantic latitudes (Auster 2005; Costello et al. 2005). This is further supported by observations of numerous fish species often in direct contact with coral surfaces, either lying on, burrowing, or sheltering within the coral framework (Ross and Quattrini 2007; Sulak et al. 2007). Such intimate contact seems less common or lacking in similar observations in other areas (e.g., of California, Tissot et al. 2006). Unique faunal associations at deep reefs coupled with a fauna typified by new range records and new species make the SEUS and GOM regions appealing for investigating species-specific, deep-reef habitat associations.
In this study, multibeam sonar and in situ video data were coupled to test whether deep-reef species exhibit affinities to particular habitat types at deep-sea reefs. Megafaunal-habitat associations were, therefore, examined at fine (10 s of m) to broad (100 s of m) spatial scales on a deep-sea coral mound off North Carolina, USA. We focused on the structural characteristics of the habitat rather than variable water mass parameters (e.g., temperature, dissolved oxygen) to facilitate comparisons with shallow-water reef studies. To determine whether megafaunal species associated with deep-sea corals were affiliated with certain habitat types: (1) habitat variables were derived from multibeam and video data, (2) dominant invertebrates and fishes were enumerated using submersible video, (3) megafaunal abundances were mapped with habitat variables, and (4) two statistical techniques were used to examine species’ distributions relative to habitat types.
Materials and methods
Coral-mound surveys
The Cape Fear (CF) coral mound, ~140 km east of Cape Fear, North Carolina (33° 34.4′N, 76° 27.8′W), was surveyed using the Johnson Sea-Link (JSL) submersible and multibeam sonar. The combination of good quality multibeam data and broad coverage of submersible dives (traversing 4.9 km) across this isolated mound made this site one of the best-surveyed deep-coral mounds in the SEUS region (Fig. 1a; for site descriptions see Partyka et al. 2007; Ross and Quattrini 2007, 2009). Nine JSL dives were conducted (2002–2005) across the mound in summer-fall (see Ross and Quattrini 2007 for more dive details). No multibeam data were available to help guide the JSL dives, so details of mound morphology could not be used to guide dives. Our overall dive objectives were to locate the coral mound, survey the habitats and fauna, and collect within Lophelia pertusa habitat. Although dives targeted Lophelia pertusa thickets, transects over other habitats were also conducted. Transects included all times when the submersible was moving across the bottom. Methods were standardized as much as possible by keeping the sub as close to the bottom as practical, maintaining slow speed, tilting the external camera downward (~30–50° toward seafloor), and videotaping on wide-angle view. The consistent camera field of view and motion of the JSL maintained consistency among dive videos so that data were comparable. For scale, two laser pointers were mounted (25 cm apart) on the camera. As a back-up, video was also recorded with a hand-held camera from the bow compartment of the JSL throughout each dive. Depth, temperature, salinity, date, and time were logged at ≤1 scan s−1 intervals using a Sea-Bird SBE 25 or 19 plus conductivity–temperature–depth (CTD) logger attached to the submersible. CTD data were overlain on the external videotapes. The submersible’s position was tracked irregularly (every 4 s to 10 min) during all dives from the surface support ship using a Trackpoint II USBL system (1% slant range error, JSL crew, HBOI, pers. comm.). Multibeam data were collected in 2006 using the Kongsberg-Simrad EM1002, a 95 kHz echosounder with 111 beams ping−1 over a maximum coverage sector of 150° (beam spacing was equidistant), mounted on the NOAA vessel Nancy Foster. Raw multibeam data were processed using CARIS HIPS and SIPS (v 6.1) to produce a 10-m-resolution bathymetric map.
Dive track processing
Post-processing of JSL dive tracks was completed to remove erroneous track data following Partyka et al. (2007). We used speed of the JSL and the depth logged by the CTD to guide the removal of erroneous positions. Given a maximum JSL speed of 1 knot (1 knot = 0.51 m s−1) (JSL crew, HBOI, pers. comm.) and a possible 1 knot current from the stern, the JSL could travel at its fastest predicted speed of 1.11 m s−1. As a conservative measure, this estimate was doubled, and location points that were more than 2.22 m s−1 away from previous locations were deleted. The location points were plotted in ArcGIS (v 9.2, ESRI) and further edited by averaging every three points along each track. Remaining dive track positions were then error-checked by viewing the internal and external JSL dive videos to ensure that depths of positions along the dive tracks obtained from the JSL CTD data matched the multibeam bathymetry. Video review also revealed whether the overall direction of travel and small-scale movements (e.g., turns, stops) of the JSL matched the plotted tracks. Although accuracy in position is important in georeferencing the dive track to the multibeam bathymetry and in obtaining habitat variables calculated from the digital terrain model, the smallest scale of terrain analysis (30 × 30 m, see “Digital terrain model analyses” section) was large enough that potential inaccuracies (<30 m) in the positional data would not influence the results.
Video analyses
Fourteen species of megafaunal fishes and invertebrates consistently associated with SEUS deep-sea reef habitats were selected for analysis. Of the 18 species of reef fishes observed on the CF mound (Ross and Quattrini 2009), eight dominant species of fishes were selected: alfonsino Beryx decadactylus (Berycidae), American conger eel Conger oceanicus (Congridae), blackbelly rosefish Helicolenus dactylopterus (Scorpaenidae), western roughy Hoplostethus occidentalis (Trachichthyidae), shortbeard codling Laemonema barbatulum (Moridae), coral hake Laemonema melanurum (Moridae), roughtip grenadier Nezumia sclerorhynchus (Macrouridae), and wreckfish Polyprion americanus (Polyprionidae). Mobile invertebrates analyzed included the following: sea urchins Echinus spp. (Echinidae), squat lobster Eumunida picta (Eumunididae), and spider crab Rochinia crassa (Epialtidae). Sessile invertebrates included the following: brisingid seastar Novodinia antillensis (Brisingidae), actinostolid anemones (Actinostolidae), and flytrap anemone Actinoscyphia saginata (Actinoscyphiidae). It was difficult to differentiate species of Echinus on video, including E. tylodes and E. gracilis, which have both been collected in the region, so these species were combined and reported as Echinus spp. In addition, A. saginata was the only species of anemone that could be accurately identified to species on video; all other anemones were identified to family.
Dominant megafaunal species were enumerated and identified to the lowest possible taxonomic level during nine dives when the JSL was transecting (see Ross and Quattrini 2007, 2009). Submersible transects were divided into 10 s segments (~5 m in length) so that variability in JSL movement (stopping to collect specimens or rapid speed) and poor video quality (zoomed or dark views) could be removed from analyses. These segments also accounted for the abrupt, fine-scale habitat changes that occurred along transects. We also deleted data if the JSL crossed its own track during any one dive. Individuals of each species were counted during each segment except actinostolid anemones, which were coded as absent, rare (1–10 individuals), common (10–100 individuals), or abundant (>100 individuals). Our counts were conservative to ensure that no individuals were counted more than once. Megafaunal abundances during each 10-s segment were georeferenced to the corrected dive track and plotted (ArcGIS v 9.2) onto CF bathymetry using time as a correlate.
Three general habitat types (modified from Partyka et al. 2007) were classified using video data and scientists’ observations: (1) soft/rubble = soft substrate with <50% rubble (dead, broken, unattached rock or bio-eroded coral pieces) coverage, (2) rubble = soft substrate with >50% rubble coverage, or (3) hard coral ≥50% coverage of intact branches or thickets of dead or live L. pertusa. No conspicuous octocorals were observed on this mound. Hard coral habitat was further differentiated by gradients of vertical profile, live coral coverage, and percent bottom coverage. Profile was characterized by coral height: low ≤0.5 m, moderate = 0.5–1 m, or high ≥1 m. Percent live coral classifications were defined as: low ≥0–10%, low-moderate ≥10–50%, moderate-high = 50–75%, or high ≥75%. Lastly, bottom coverage was measured by percent of seafloor covered by hard corals: low ≤50%, moderate = 50–75%, or high ≥75%. Time was recorded when the habitat changed. Using these times as correlates, habitat data were georeferenced to the dive tracks and then mapped (ArcGIS) using the Inverse Distance Weighted interpolation to 30 m on either side of each dive track to facilitate map readability.
Digital terrain model analyses
A digital terrain model (DTM) of the CF mound was created (ArcGIS) from the 10-m-resolution multibeam bathymetry (Fig. 2b) and used to calculate habitat variables at two spatial scales. Habitat variables were calculated across the DTM at fine (30 × 30 m) and broad (170 × 170 m) spatial scales using Landserf 2.2 (Wood 2005) and ArcGIS extension Benthic Terrain Modeler (NOAA, Oregon State University) software programs. Habitat variables (Table 1) calculated with these software packages included: aspect, bathymetric position index (BPI), curvature, fractal dimension, rugosity, and slope. Altitude was calculated by subtracting the depth of the JSL recorded by the CTD from average bottom depth at the base of the mound determined using the DTM.
Habitat calculations at two spatial scales were performed using a sliding window analysis. Analysis windows were based on the resolution of the multibeam data (Albani et al. 2004; Hartley et al. 2004; Wilson et al. 2007). Each pixel (10 m) on the DTM became a centroid in the analysis window, and the perimeter surrounding the central pixel consisted of either 3 or 17 pixels in both the x and y directions. The size of each analysis window was determined by multiplying the resolution (10 m) of the multibeam data by the number of pixels (e.g., 3 × 10 m = 30 m and 17 × 10 m = 170 m). Therefore, 30 × 30 m and 170 × 170 m analysis windows were established as fine and broad spatial scales, and habitat variables were then calculated within these areas across the DTM. Because of the multibeam resolution, 30 × 30 m was the smallest possible area in which a habitat variable could be calculated. This was also the smallest practical scale because a 1-pixel analysis window is unsatisfactory as it could capture the elevation errors that can occur within a DTM (Albani et al. 2004). The 170 × 170 m window was determined to be an appropriate measure for broad-scale analysis using measures of fractal dimension (Hartley et al. 2004; Wilson et al. 2007; Dolan et al. 2008). Fractal dimension (surface complexity) values at different spatial scales can denote the boundary between fine- and broad-scale terrain properties (Hartley et al. 2004; Wilson et al. 2007). We calculated fractal dimension in analysis windows of 9, 17, 33, and 65 pixel sizes. At window sizes >17 pixels, fractal dimension values changed little, so 17 pixels were used to denote the break between fine and broad-scale terrain properties (Hartley et al. 2004; Wilson et al. 2007).
Statistical analyses
Associations between megafaunal species and habitat variables were statistically analyzed using canonical correspondence analysis (CCA, Canoco v 4.5 [see ter Braak 1996; ter Braak and Šmilauer 2002]). CCA explains species distribution patterns by calculating the species centroid, or optimum location, among habitat variables and functions well with datasets that contain numerous zero values (ter Braak 1986, 1996). We considered the 10-s segments per dive as independent samples because fine-scale habitats and/or depth changed from segment to segment. Abundances per segment were square-root transformed to downweight abundant compared to rare individuals. Altitude and rugosity were log (X + 1) transformed to reduce skewed data distribution. Because negative values occurred in the BPI data, BPI values were standardized by adding a constant to each value to move the minimum value of the distribution to 1.0 before a log (X + 1) transformation was applied. Two CCA tests were performed; one test included habitat data from video and from 30 × 30 m DTM calculations, and the other test only included habitat data from 170 × 170 m DTM calculations. For each test, the biplot scaling option on inter-species distances was selected, and a Monte Carlo permutation tested significance of the first canonical eigenvalue and the sum of all canonical eigenvalues. Simple ordination plots were created (CanoDraw for Windows 4.4), displaying weighted averages of each species along categorical habitat types (as points) and quantitative habitat variables (as arrows, ter Braak 1986). The length of each arrow is correlated to the ordination axes, in that a longer arrow indicates a stronger correlation and explains more variation in species’ distribution patterns (ter Braak 1986). The resulting ordination diagrams combined with the low eigenvalues prompted us to perform two additional CCA tests that were similar to the first two tests except that they included fish abundance data only.
A second multivariate analysis based on eigensystem computation, ecological niche factor analysis (ENFA; Biomapper v 4.0, [Hirzel et al. 2004]), was used to supplement the CCA and obtain additional information on megafaunal-habitat associations. ENFA compares the observed distribution of a species within localities characterized by particular habitat conditions to a reference set of habitat variables describing the whole study area. Although ENFA results cannot infer causality, they can indicate which habitat conditions are highly associated with the observed species distribution (Hirzel et al. 2002). Deep-sea data are often limited, so ENFA is particularly useful because this analysis only requires presence data (Hirzel et al. 2002; Wilson et al. 2007).
ENFA was conducted on each species, except for Hoplostethus occidentalis because <10 individuals were observed. Landserf and ArcGIS raster grids of mapped habitat variables and abundance data were first imported into Biomapper. Floating point raster grids were then converted into an Idrisi (Eastman 1997) format using the Biomapper conversion tool and manual modification of document reference files. The Box-Cox function was applied to the quantitative habitat variables derived from the DTM in the Biomapper program to reduced skewed data. Species abundance data were analyzed as a Boolean dataset. Anemones were analyzed using the weighted species presence option in Biomapper because they were qualitatively coded into abundance categories.
ENFA results provide information on the habitat specificity of each species in the form of marginality and specialization output factors (see Hirzel et al. 2002). Marginality, explained by the first output factor, reveals how the distribution of a species differs from the mean habitat conditions of the study area. The higher the absolute value of each habitat coefficient, the further the distribution of a species departs from the mean value of that particular habitat variable. A negative value indicates the species is found in areas with values for habitat variables lower than the mean value. A positive value indicates a species is found in areas where values for habitat variables are higher than the mean value. The first output factor also explains a portion of the specialization (reported as %, Table S1), which describes the specificity of a species to a particular habitat value. The remaining output factors also indicate the degree of specialization of a species toward a particular habitat variable where higher absolute values of coefficients indicate a species will more likely be found within a particular habitat range. Biomapper also computes global marginality, specialization, and tolerance values. Global marginality values are generally 0–1; a value closer to 1 indicates that a species is found in habitats where conditions differ significantly from the mean of all habitats surveyed. Global specialization ranges from 1 to ∞; a higher number indicates greater specialization to a particular habitat within the study area. Finally, global tolerance values range from 0 to 1, with higher values indicating that a species has a more widespread distribution within the study area than expected for habitat specialists.
Results
Cape fear mound habitat
The CF coral mound is ~0.7 km2, exhibiting slopes up to 80° and rising ~100 m from the surrounding seafloor. This biogenic mound appears to have been created by the successive growth, collapse, and sediment entrapment of Lophelia pertusa. The top of the mound exhibits double peaks with one at 366 m and the other at 374 m depth. The average depth around the base of the mound is 463 m (450–480 m range; Fig. 1b). A tear-drop-shaped trench, seemingly current-scoured, with the narrow end facing northward occurs around the base of the mound. Although habitats were patchy, low profile, dead (90–100%), L. pertusa with >75% bottom coverage was the dominant fine-scale habitat type observed on the mound, particularly on the slope and the top (Fig. 2). Most of the moderate-to-high-profile, hard coral habitat was observed at ~5–45 m from the top of the mound and appeared to be concentrated on the south-southwest (up-current) facing slope (Fig. 2). The majority of the live coral was also concentrated in these areas where high-profile coral colonies were observed. Conversely, the north-northeast slope of the mound was covered with mostly soft/rubble, rubble, and low profile, dead (90–100%) hard coral habitats (Fig. 2).
Habitat values calculated at the 30 × 30 m and 170 × 170 m scales revealed fine- to broad-scale habitat changes across the mound (see Figs. S1–3). At the fine scale, the steepest gradient in slope (up to 86°) was observed on the south-southwest side of the mound, whereas the slope on the north-northeast side of the mound was more gradual. Broad-scale (170 × 170 m) slope values showed the same pattern as fine-scale slope values, but were calculated to be much lower (up to 25°). Rugosity and fractal dimension values indicated that the most complex terrain occurred on top of the 374-m peak and along the 420-m contour on the west and south-west sides of the mound. Finally, measures of curvature and BPI indicated that very few fine-scale crests, ridges, and valleys occurred across the mound; except that along-slope ridges following bathymetric contours (380–420 m) were common near the top on the south-southwest facing slope. At the broad scale, little variability was observed in curvature and BPI; rather these measurements illustrated the topographic highs and lows of the whole mound.
Species-habitat data
Fourteen dominant invertebrate and fish species were enumerated during 4 h of transect time (1,453 10-s segments) from the nine JSL dives. Dominant fishes were, in decreasing order of abundance, as follows: Beryx decadactylus (n = 69 individuals), Laemonema barbatulum (n = 23), Conger oceanicus (n = 18), Helicolenus dactylopterus (n = 12), Nezumia sclerorhynchus (n = 12), Polyprion americanus (n = 11), Laemonema melanurum (n = 10), and Hoplostethus occidentalis (n = 8). Dominant mobile invertebrates were, in decreasing order of abundance, as follows: Eumunida picta (n = 1,517), Echinus spp. (n = 545), and Rochinia crassa (n = 13). Actinostolid anemones, Actinoscyphia saginata (n = 3,350), and Novodinia antillensis (n = 16) were the most abundant sessile species attached to L. pertusa colonies. Although abundances were low for some species, they were included in analyses because little data exist on the majority of these species.
The species-habitat relationships were significant (CCA, Monte-Carlo, P = 0.002) in the CCA tests that included all megafaunal species. Also, results were similar between the CCA tests that included either habitat variables calculated at the fine scale or variables calculated at the broad scale. Approximately 60–70% of the variance in species-habitat data was explained by the first two canonical axes (Table 2). However, eigenvalues were small, and correlations were moderate for each canonical axis when all species were included in analyses. Reanalysis of the datasets using only the fish data yielded considerably higher eigenvalues and correlations (Table 2). Thus, the species-habitat relationship was strengthened, suggesting that fishes analyzed in this study showed greater affinity to habitat variables than the invertebrates. In addition, global specialization values for fishes were generally higher than those for most invertebrates, further supporting that fishes were more habitat-specific than the invertebrates observed in this study (ENFA, Table 3).
Both Beryx decadactylus and Conger oceanicus were most frequently observed on the south-southwest facing slope near the top of the coral mound (reef crest) (Figs. 3, 4a, b). These two species were associated with high-profile, hard coral habitat that had low-moderate to moderate-high percentages of live coral cover (CCA, Figs. 3, 4a, b). CCA also revealed that high values of BPI calculated at the fine scale, and high values of mean and profile curvature calculated at the broad scale were important habitat variables, indicating that these two species were associated with topographic highs (CCA, Fig. 3). ENFA (see Table S1) results were consistent with CCA, except ENFA also indicated that C. oceanicus was associated with high values of fractal dimension, suggesting that this species had an affinity for rough terrain. Conger oceanicus was frequently observed on the 374-m peak and along the most northern dive track where complex terrain was more common (Fig. 4b). In addition, C. oceanicus was often observed protruding from holes within the coral matrix. Both B. decadactylus and C. oceanicus had the highest marginality values of all species examined, indicating that these species occurred in habitats that differed most from the mean habitat conditions at the CF mound (Table 3). These preferred habitats of B. decadactylus and C. oceanicus, topographic highs of high-profile, live hard coral, were not as common as the lower profile, dead hard coral habitat that covered the majority of the mound (Fig. 2).
Laemonema melanurum had a similar distribution to B. decadactylus and C. oceanicus, except L. melanurum was often observed in moderate profile habitats and not as often in live coral habitats (CCA, Figs. 3, 4f). ENFA results were consistent with CCA results (Table S1). The global marginality value (0.54) indicated that this species occurred in habitat conditions that differed somewhat from the mean habitat conditions on the mound (ENFA, Table 3). This result is likely due to the association of L. melanurum with uncommon moderate-to-high-profile fine-scale habitats, as well as dead (90–100%) hard coral with 90–100% bottom coverage (Fig. 2), a commonly observed fine-scale habitat.
Hoplostethus occidentalis and Laemonema barbatulum were prevalent on steeper slopes on the north-northeast sides of the coral mound (CCA, Figs. 3, 4d, e). Hoplostethus occidentalis was restricted to this side of the mound, whereas L. barbatulum was also observed on the top of the mound and at the base of the south-southwest facing slope. These two species were often found in hard coral habitats generally characterized by low to moderate profile with a low percentage of bottom coverage near the base of the mound (CCA, Fig. 3). Associations with the base of the mound were indicated by affinities for low values of curvature and BPI calculated on the broad scale (Figs. 3, 4d, e). ENFA results were consistent with CCA (Table S1). A high global marginality value indicated that L. barbatulum occurred in a range of habitat conditions that were different from the mean conditions at the mound (ENFA, Table 3). This was most likely due to associations with fine-scale habitats not commonly observed on the mound (Fig. 2), such as a low percentage of hard coral bottom coverage, and rubble and sand/rubble habitats.
Helicolenus dactylopterus exhibited a distribution similar to H. occidentalis and L. barbatulum; however, H. dactylopterus was also frequently observed on the southwest facing slope (Figs. 3, 4c). ENFA results were similar to CCA results (Table S1), and further supported associations of H. dactylopterus with high slope values. A moderate global marginality value indicated that H. dactylopterus was often associated with habitat that was fairly common on the mound (ENFA, Table 3), such as low profile, dead (90–100%) hard coral habitat. However, this species was also observed in rubble habitat that was not as common as hard coral habitat.
Polyprion americanus and Nezumia sclerorhynchus were observed in numerous habitat types (Figs. 3, 4g, h). Of all fishes observed in this study, these two species exhibited the least affinities for a particular habitat type (CCA, Figs. 3, 4g, h). ENFA results, however, revealed that P. americanus and N. sclerorhynchus were associated with the south-southwest side of the mound (Table S1). This association can be seen in the abundance maps (Fig. 4g, h), but was not revealed in the CCA analysis (Fig. 3). Overall, global marginality values were the lowest of all fishes analyzed in this study, indicating that these two species were associated with habitat types that were common on the coral mound (ENFA, Table 3).
In general, invertebrates showed less affinity to particular habitat variables than fishes. The brisingid seastar, Novodinia antillensis, was the only exception, sharing a distribution similar to Beryx decadactylus and Conger oceanicus on the south-southwest facing slope near the top of the mound in high-profile, hard coral habitat with moderate-to-high live coral coverage (CCA, Figs. 3, 4m). Furthermore, individuals were notably absent from rubble and soft substrate habitats and were more frequently observed on branches of live coral compared to other invertebrates. ENFA results were consistent with CCA (ENFA, Table S1). Novodinia antillensis had the highest marginality and specialization values of all invertebrates analyzed in this study (ENFA, Table 3). These high values indicated that this species was found in particular habitat types that were not commonly observed on the coral mound: topographic highs of high-profile live hard coral.
The other mobile, megafaunal invertebrates were present in various areas across the mound and rarely showed affinities to particular habitat types. These invertebrates exhibited no particular associations with either high or low values of habitat variables in the CCA plot (Fig. 3). ENFA results and the distribution maps, however, indicated that a few habitat variables may influence the distribution of invertebrates. Higher altitude appeared to be important for invertebrates, as invertebrates were most abundant near the top of the coral mound (ENFA, Table S1). Most invertebrates examined here were generally absent or rare at the base of the mound and rarely observed on coral rubble or soft substrate (Figs. 3, 4i–n). Additionally, actinostolid anemones and A. saginata were concentrated on the south-southwest-facing slopes. Echinus spp. and E. picta were associated with higher percent coverage of coral and higher values of BPI and curvature, indicating associations with topographic highs (ENFA, Table S1). ENFA indicated that the north-northeast side of the mound was an important habitat variable for Rochinia crassa; however, the distributional maps indicated that this species was observed in all areas of the mound. Global ENFA values were similar among invertebrates and also revealed associations with habitat conditions that were most similar to the common habitat conditions on the mound (Table 3).
Discussion
Knowledge of species’ relationships to habitat variables and the degrees to which species depend on fine-scale habitat features are important for understanding habitat functionality, predicting faunal distribution and abundance patterns, and for assessing the impacts of habitat disturbance (Wilson et al. 2008). Habitat affiliations of fishes and mobile, megafaunal invertebrates on deep-sea coral reefs have been documented at fairly broad scales, such as reef versus non-reef or coral versus non-coral (e.g., Mortensen et al. 1995; Auster 2005; Ross and Quattrini 2007). Linking broad habitat classifications to species’ distributions has provided insight into occurrence and biodiversity patterns of species inhabiting different deep-reef substrates (rock or coral) and nearby habitats; however, a gap in our understanding of fine-scale habitat functionality remains (Roberts et al. 2008). This is in stark contrast to shallow-water reef ecosystems, where faunal affiliations with fine-scale habitat features, such as topographic complexity, proportion of live coral, and vertical profile, are well studied. This study shows that deep-reef megafauna are affiliated with particular fine-scale habitats, and that certain reef-associated species appear to be habitat specialists, whereas others are habitat generalists.
Species-habitat associations
At the broad scale, most species, including the majority of live Lophelia pertusa colonies, were often distributed within a particular reef zone: near the top of the CF mound on the south-southwest facing slope. This area of the mound, the reef crest, represents the up-current side directly impacted by the northward flowing Gulf Stream. Although there are no long-term oceanographic data from the CF mound, physical data from nearby moorings (~400 m depth) indicate that the predominant current direction on the bottom is northward, but that reversing currents and upwellings also impact these depths due to Gulf Stream meanders (Brooks and Bane 1983; J. Bane, pers. comm.). Also, the teardrop-shaped scoured trench around the base of the mound suggests a predominant northward bottom current. In addition to facing the current, the south-southwest slope near the top of the mound experiences accelerated current speeds as the current flows over the mound (pers. observ.). The interaction of topographic highs with accelerated currents, internal waves, and tidal signals enhances food supply to cold-water corals (Genin et al. 1986; Frederiksen et al. 1992; Thiem et al. 2006; Davies et al. 2009). We propose that the preferential occupation of the elevated up-current side of the CF mound is related to enhanced feeding opportunities.
The Gulf Stream likely influences the broad-scale distributions of benthic suspension feeders (e.g., L. pertusa, Novodinia antillensis, anemones) and species that actively prey in the water column. The interplay between currents and elevated topography is complex, enhancing feeding in different ways depending on feeding mode. Mobile megafauna that aggregated near the top of the coral mound are likely exploiting both the enhanced food supply delivered by the Gulf Stream as well as the vertically migrating, mesopelagic fauna that impinge on the bottom during the day (Gartner et al. 2008). Eumunida picta (as well as other species of squat lobsters, Wilson et al. 2007) prefers the tops of coral mounds and coral colonies. This species actively feeds on mesopelagic fauna when those organisms are near the bottom (pers. observ.). Beryx decadactylus also feeds mostly in the water column on mesopelagic fishes, pelagic shrimp, squid, and pelagic tunicates (Goldman and Sedberry 2011). This species may even follow mesopelagic fauna up into the water column at night (Gomes et al. 1998). Echinus spp. could be exploiting the phytodetrital material (Campos-Creasey et al. 1994) delivered by currents to the top of the mound.
Current speed and direction and elevated topography may be less important to species that do not require these factors for enhanced feeding opportunities. For example, the transient reef species, Laemonema barbatulum, was not particularly associated with the top of the coral mound, and was commonly found off-reef over soft substrate along the upper slope in the region (Quattrini and Ross 2006; Ross and Quattrini 2007). The distribution of this species relative to broad-scale habitat features, such as reef slope and flat, may be driven by a diet consisting of infaunal and benthic invertebrates (Gartner et al. 1997; Weaver and Sedberry 2001). Rochinia crassa was also not highly associated with a particular reef zone or other habitat characteristics. Like other species of Rochinia (Cordes et al. 2005), R. crassa is a likely scavenger; thus, this species would not be constrained to elevated topography or high-current areas.
Certain species were also predictably associated with fine-scale habitat features. For example, the distributions of Beryx decadactylus, Conger oceanicus, and Novodinia antillensis were influenced by vertical profile, high proportion of live coral, high percentage of coral coverage, and topographic complexity. These habitat variables are interrelated, and most commonly found at the top of the mound where live coral growth is enhanced. Species’ associations with these fine-scale habitat types are not an artifact of location because these species were not observed in low-profile coral areas that also occurred on the top of the mound (and on other coral mounds off the SEUS, pers. observ.). Beryx decadactylus was predictably associated with high-profile coral and often observed under rock ledges and coral thickets throughout deep-water habitats in the region (pers. observ.). Ledges, undercuts, and holes that are often associated with high vertical profile provide refugia from strong currents and predators as well as sites for reproduction and feeding (e.g., Hixon 1991; Menard et al. 2007). Conger oceanicus is also known to forage at night and utilize refugia during the day (Levy et al. 1988). In addition to burrowing in thickets of L. pertusa, C. oceanicus inhabits tilefish burrows, “pueblo habitats” in the walls of submarine canyons (Levy et al. 1988), and shipwreck components (pers. observ.). Ross and Quattrini (2009) noted that the high-relief profile provided by the Republic shipwreck, at ~490 m depth off Georgia, attracted both C. oceanicus and B. decadactylus. The high-relief structure was hypothesized to be the most important factor driving the fish assemblage similarity between North Carolina L. pertusa reefs and the Republic shipwreck. Finally, N. antillensis was predictably associated with high-profile habitat and often observed perched on the tops of live coral colonies. Occupying the highest profile habitats near the top of the mound in areas of the strongest currents would promote optimal feeding opportunities for this suspension feeder (Emson and Young 1994). Thus, the availability of extensive, complex, high-relief L. pertusa thickets or other similar structures appears to influence the distribution of C. oceanicus, B. decadactylus, and N. antillensis.
Shallow-water comparisons
The abundance and/or species richness of shallow-water (<200 m) reef megafauna are often highly correlated with various physical habitat parameters. Parameters that influence shallow-water fish and invertebrate species include habitat complexity (e.g., Luckhurst and Luckhurst 1978), vertical structure (e.g., Gratwicke and Speight 2005), reef size and type (e.g., Abele and Patton 1976; Ault and Johnson 1998a; Alexander et al. 2009), reef zone (e.g., Wilson 2001), proportion of live coral (e.g., Bell and Galzin 1984), and/or depth (e.g., Ault and Johnson 1998b; Friedlander and Parrish 1998). The distribution of mobile invertebrates is also often related to the type and size of sessile invertebrates as numerous species, many symbiotic, seek refuge in sponges (Henkel and Pawlik 2005), anemones (Nizinski 1989), and sea fans (Kissling and Taylor 1977). Moreover, abundances and distributions of species can vary due to the level of specialization that a species has to a certain habitat variable (Munday et al. 1997), highlighting the importance of documenting habitat associations at the species level. Although fine-scale habitat associations are lacking for the majority of deep-reef species, a few recent studies have indicated that certain mobile megafaunal invertebrates are closely tied to fine-scale, deep-reef habitat types such as coral species type and vertical relief (Mosher and Watling 2009; Lessard-Pilon et al. 2010). Our results also indicate that some deep-sea species are similarly influenced by habitat variables as shallow-water reef fauna, at least within the SEUS region.
Reef zone is one parameter often associated with shallow-water faunal distributions as successful feeding, recruitment, and/or competitive interactions often result in organisms occurring within a particular zone (e.g., reef crest, slope, flat [Robertson and Gaines 1986; Munday et al. 1997; Friedlander and Parrish 1998; Wilson 2001]). For example, detritivores were more abundant on shallow-water reef crests compared to other reef zones because of the higher quality and availability of particulate organic matter on the reef crest (Wilson 2001). Food resource availability may similarly drive the observed distribution patterns within particular zones at the CF mound. As noted above, several suspension feeders, detritivores, and mesopelagic feeders were found on the south-southwest facing slope near the top of the mound (or the reef crest). In contrast, most transient (not limited to primary reef occupancy) reef species (Laemonema barbatulum, Nezumia sclerorhynchus, Helicolenus dactylopterus, Rochinia crassa, and Polyprion americanus) were either associated with reef slope, reef flat, or lacked strong association with any particular zone. Transient shallow-water fishes are common in these zones, presumably for foraging, and often have limited affinities with topographic complexity (Friedlander and Parrish 1998). Further work is needed to understand the trophic dynamics of deep-reef megafauna, but our results suggest that local distributions of deep-reef species may change across reef zones according to their trophic guild as has been suggested in shallow-water reef studies (Friedlander and Parrish 1998; Wilson 2001).
Several species were affiliated with increased topographic complexity and high vertical profile, two reef parameters that create refugia for shallow-water species (Hixon 1991; Friedlander and Parrish 1998; Menard et al. 2007). Predation pressure is the primary factor driving refugium use on shallow-water reefs (Hixon and Beets 1993; Beck 1995; Friedlander and Parrish 1998). Likewise, invertebrates may seek refuge in complex structures on deep reefs to escape predation, particularly when molting or reproducing (Beck 1995). Both adult and juvenile squat lobsters have been collected from deep within coral thickets and coral rubble matrices along SEUS deep reefs (pers. observ.) and are often a common prey item of deep-reef associated fishes (e.g., Goldman and Sedberry 2011). For fishes, it is interesting to note that both shallow- and deep-water species utilizing refugia belong to the same orders (e.g., anguilliforms and beryciforms), and this appears to be a common trait in these taxonomic groups. Alternatively, these species may use refugia to maintain position in an optimal feeding location. Occupying refugia would lessen the metabolic costs of actively swimming in strong currents between feeding periods.
Proportion of live coral and percent cover of coral, rock, or rubble are two additional habitat variables that often influence reef species’ distributions. On shallow-water reefs, there are numerous highly specialized species that have obligate relationships with live corals (symbionts), including species that feed on corals. Live coral specialists appear to be less common on deep compared to shallow-water reefs, although a few occurrences have been reported: the corallivorous gastropod Coralliophila sp. (Cordes et al. 2008), corallivorous hippasterine seastars (Mah et al. 2010), the reef-aggregating polychaete Eunice norvegica (Roberts 2005), and the coral-specific resident ophiuroid Ophiocreas oedipus (Mosher and Watling 2009). At the CF mound, megafauna were strongly associated with the proportion of seafloor covered with coral, whether live or dead. Other observations of deep-water slope megafauna also suggested that the proportion of live coral may not be as important as the reef structure itself (Auster 2005). In fact, higher proportions of dead coral are often positively correlated with high diversity of macrofauna (Cordes et al. 2008) and megafauna (e.g., Roberts et al. 2008), although species assemblages associated with dead coral rubble and primary coral framework often differ (Jonsson et al. 2004; Ross and Quattrini 2007). While living coral is often considered a sign of a healthy ecosystem, it does not necessarily predict the biodiversity and abundance at deep reefs. Seafloor covered with a high proportion of dead hard coral thickets provides the necessary structure to support primary reef occupants (Harter et al. 2009). Furthermore, dead coral rubble around the reef is an important component of the deep-reef ecosystem that provides habitat for a diverse, yet different faunal assemblage.
Within the North Atlantic Ocean, the degree to which fishes are specifically associated with habitats might be similar between shelf and slope environments. The characteristic deep-reef ichthyofauna along the continental slopes of the SEUS and GOM (Ross and Quattrini 2007, 2009; Sulak et al. 2007) appears to mirror the habitat affinities of the sub-tropical hardbottom fauna along the shelf and shelf-edge in these regions (e.g., Quattrini and Ross 2006; Kendall et al. 2009). Conversely, many fish species in the higher, colder latitudes of the North Atlantic Ocean appear to exhibit less affinity for microhabitat reef structure at shelf depths (Auster et al. 1995) and also on deep reefs (Auster 2005; Costello et al. 2005). The degree of habitat specialization might decrease with increasing latitude regardless of depth (at least <1,000 m). Further work using similar methodology is needed to test whether this change in habitat specialization among deep-reef fauna corresponds to changes with latitude, as noted for several different faunal groups ranging from terrestrial to shallow-water marine environments (see Stevens 1989).
Further considerations
Understanding faunal habitat affiliations on coral reefs requires examination at multiple spatial scales because different ecological, biological, and physical processes influence the distributions of species (e.g., Albani et al. 2004; Wilson et al. 2007). Environmental variables such as temperature, surface water productivity, and dissolved oxygen could affect the distribution of fauna at broad spatial scales. Such data should be explored in future work, but it should be recognized that frequently measured long-term data are needed to fully capture the oceanographic conditions and their variability at deep coral mounds (Davies et al. 2009, 2010).
This study excluded microscale analyses of habitat associations, and our scale choices may have been too broad to determine invertebrate habitat affiliations, although they were appropriate in depicting fish distributions. Species-specific associations between mega-invertebrates (e.g., ophiuroids, shrimp) and substrates, such as anemones, sponges, octocorals, and antipatharians, are common and sometimes obligate (e.g., Nizinski 1989; Henkel and Pawlik 2005; Mosher and Watling 2009), emphasizing the importance of examining the relationships between these invertebrates and their preferred substrate type. Although video methodology worked well for quantifying the larger, mobile species, the smaller, more cryptic species deep within the reef matrix, such as ophidiiform fishes (Nielsen et al. 2009), ophiuroids (Brooks et al. 2007), and several species of squat lobsters (M. Nizinski, unpubl data) were not well documented. These smaller-scale associations may be better documented with zoomed-in video data and digital still imagery.
This study indicates greater habitat specificity of deep-reef megafauna than previously documented, supporting and expanding the work by Ross and Quattrini (2007, 2009). Some species are habitat generalists, while several deep-reef, mobile megafaunal species are habitat specialists. Our detailed examination of faunal-habitat associations at the CF coral mound in the SEUS region provides a basis for testing hypotheses concerning faunal-habitat relationships and community assembly processes at deep-sea reefs in other locations. Habitat usage at deep-water reefs likely changes within a region, depending upon such factors as habitat diversity, food availability, predator occurrence, depth of coral mound, competition, migration patterns, recruitment dynamics, and evolutionary history. Within the SEUS, we hypothesize that at broad spatial scales, distributions of deep-reef megafauna are governed by food availability and depth of coral mound. These distributions, however, will be influenced on the fine-scale by topographic complexity, vertical relief, and coral coverage. Future work should concentrate on multi-scale, multi-species analyses on other reef habitats along the continental slope to determine processes that influence the formation, stability, and connectivity of deep reef communities.
References
Abele LG, Patton WK (1976) The size of coral heads and the community biology of associated decapod crustaceans. J Biogeogr 3:35–47
Albani M, Klinkenberg B, Andison DW, Kimmins JP (2004) The choice of window size in approximating topographic surfaces from digital elevation models. Internat J Geogr Inform Sci 18:577–593
Alexander TJ, Barrett N, Haddon M, Edgar G (2009) Relationships between mobile macroinvertebrates and reef structure in a temperate marine reserve. Mar Ecol Prog Ser 389:31–44
Anker A, Nizinski M (2011) Description of a new deep-water species of Alpheus Fabricius, 1798 from the Gulf of Mexico (Crustacea, Decapoda, Alpheidae). Zootaxa 2925:49–56
Attrill MJ, Strong JA, Rowden AA (2000) Are macroinvertebrate communities influenced by seagrass structural complexity? Ecography 23:114–121
Ault TR, Johnson CR (1998a) Spatial variation in fish species richness on coral reefs: habitat fragmentation and stochastic structuring processes. Oikos 82:354–364
Ault TR, Johnson CR (1998b) Spatially and temporally predictable fish communities on coral reefs. Ecol Mono 68:25–50
Auster PJ (2005) Are deep-water corals important habitats for fishes? In: Freiwald A, Roberts JM (eds) Cold-water corals and ecosystems. Springer, Berlin, pp 747–760
Auster PJ, Malatesta RJ, LaRosa SC (1995) Patterns of microhabitat utilization by mobile megafauna on the southern New England (USA) continental shelf and slope. Mar Ecol Prog Ser 127:77–85
Beck MW (1995) Size-specific shelter limitation in stone crabs: a test of the demographic bottleneck hypothesis. Ecology 76:968–980
Bell JD, Galzin R (1984) Influence of live coral cover on coral reef fish communities. Mar Ecol Prog Ser 15:247–265
Brooks DA, Bane J (1983) Gulf Stream meanders off North Carolina during winter and summer 1979. J Geophys Res 88:4633–4650
Brooks RA, Nizinski MS, Ross SW, Sulak KJ (2007) Frequency of sublethal injury in a deepwater ophiuroid, Ophiacantha bidentata, an important component of western Atlantic Lophelia reef communities. Mar Biol 152:307–314
Buhl-Mortensen L, Vanreusel A, Gooday AJ, Levin LA, Priede IG, Buhl-Mortensen P, Gheerardyn H, King N, Raes M (2010) Biological structures as a source of habitat heterogeneity and biodiversity on the deep ocean margins. Mar Ecol 31:21–50
Campos-Creasey LS, Tyler PA, Gage JD, John AWG (1994) Evidence for coupling the vertical flux of phytodetritus to the diet and seasonal life history of the deep-sea echinoid Echinus affinis. Deep-Sea Res I 41:369–388
Caruso JH, Ross SW, Sulak KJ, Sedberry GR (2007) Deep-water chaunacid and lophiid anglerfishes (Pisces: Lophiiformes) off the south-eastern United States. J Fish Biol 70:1015–1026
Casazza TL, Ross SW (2008) Fishes associated with pelagic Sargassum and open water lacking Sargassum in the Gulf Stream off North Carolina. Fish Bull 106:348–363
Cordes EE, Hourdez S, Predmore BL, Redding ML, Fisher CR (2005) Succession of hydrocarbon seep communities associated with the long-lived foundation species Lamellibrachia luymesi. Mar Ecol Prog Ser 305:17–29
Cordes EE, McGinley MP, Podowski EL, Becker EL, Lessard-Pilon S, Viada ST, Fisher CR (2008) Coral communities of the deep Gulf of Mexico. Deep-Sea Res I 55:777–787
Costello MJ, McCrea M, Freiwald A, Lundalv T, Jonsson L, Brett BJ, van Weering TCE, de Haas H, Roberts JM, Allen D (2005) Role of cold-water Lophelia pertusa coral reefs as fish habitat in the NE Atlantic. In: Freiwald A, Roberts JM (eds) Cold-water corals and ecosystems. Springer, Berlin, pp 771–805
Davies AJ, Duineveld GCA, Lavaleye MSS, Bergman MJN, vanHaren H, Roberts JM (2009) Downwelling and deep-water bottom currents as food supply mechanisms to the cold-water coral Lophelia pertusa (Scleractinia) at the Mingulay Reef complex. Limnol Oceanogr 54:620–629
Davies AJ, Duineveld GCA, van Weering TCE, Mienis F, Quattrini AM, Seim HE, Bane JE, Ross SW (2010) Short-term environmental variability in cold-water coral habitat at Viosca Knoll, Gulf of Mexico. Deep-Sea Res I 57:199–212
Dolan MFJ, Grehan AJ, Guinan JC, Brown C (2008) Modeling the local distribution of cold-water corals in relation to bathymetric variables: adding spatial context to deep-sea video data. Deep-Sea Res I 55:1564–1579
Eastman JR (1997) Idrisi for windows. Version 2.0. Clark University Laboratory. Clark University Worcester, PA
Emson RH, Young CM (1994) Feeding mechanism of the brisingid starfish Novodinia antillensis. Mar Biol 118:433–442
Evans IS (1980) An integrated system of terrain analysis and slope mapping. Zeitschrift für Geomorphologie Suppl-Bd 36:274–295
Fernholm B, Quattrini AM (2008) A new species of Hagfish (Myxinidae: Eptatretus) associated with deep-sea coral habitat in the Western North Atlantic. Copeia 2008:126–132
Frederiksen R, Jensen A, Westerberg H (1992) The distribution of the scerlactinian coral Lophelia pertusa around the Faeroe Islands and the relation to internal tidal mixing. Sarsia 77:157–171
Friedlander AM, Parrish JD (1998) Habitat characteristics affecting fish assemblages on a Hawaiian coral reef. J Exp Mar Biol Ecol 222:1–30
Gartner JV, Crabtree RE, Sulak KJ (1997) Feeding at depth. In: Randall DJ, Farrel AP (eds) Deep-sea fishes. Academic Press, San Diego, pp 115–193
Gartner JV, Sulak KJ, Ross SW, Necaise AM (2008) Persistent near-bottom aggregations of mesopelagic animals along the North Carolina and Virginia continental slopes. Mar Biol 153:825–841
Genin AP, Dayton PK, Lonsdale PF, Spiess FN (1986) Corals on seamount peaks provide evidence of current acceleration over deep-sea topography. Nature 322:59–61
Goldman SF, Sedberry GR (2011) Feeding habits of some demersal fish on the Charleston Bump off the southeastern United States. ICES J Mar Sci 68:390–398
Gomes TM, Sola E, Gros MP, Menezes G, Pinho MR (1998) Trophic relationships and feeding habits of demersal fishes from the Azores: importance to multispecies assessment. ICES J Mar Sci 35:7–21
Gratwicke B, Speight MR (2005) The relationship between fish species richness, abundance and habitat complexity in a range of shallow tropical marine habitats. J Fish Biol 66:650–667
Harter SL, Ribera MM, Shepard AN, Reed JK (2009) Assessment of fish populations and habitat on Oculina Bank, a deep-sea coral marine protected area off eastern Florida. Fish Bull 107:195–206
Hartley S, Kunin WE, Lennon JJ, Pocock MJO (2004) Coherence and discontinuity in the scaling of species’ distribution patterns. Proc R Soc Lond B 271:81–88
Henkel TP, Pawlik JR (2005) Habitat use by sponge-dwelling brittlestars. Mar Biol 146:301–313
Henry LA, Nizinski MS, Ross SW (2008) Diversity, distribution, and biogeography of hydroid assemblages collected from deep-water coral habitats off the southeastern United States. Deep-Sea Res I 55:788–800
Hirzel AH, Hausser JDC, Perrin N (2002) Ecological-niche factor analysis: how to compute habitat-suitability maps without absence data? Ecology 83:2027–2036
Hirzel AH, Hausser J, Perrin N (2004) Biomapper 3.1. Lab. of conservation Biology, Department of Ecology and Evolution, University of Lausanne. URL: http://www.unil.ch/biomapper
Hixon MA (1991) Predation as a process structuring coral reef fish communities. In: Sale PF (ed) The ecology of fishes on coral reefs. Academic Press, San Diego, pp 475–508
Hixon MA, Beets JP (1993) Predation, prey refuges, and the structure of coral reef fish assemblages. Ecol Mono 63:77–101
Jenness J (2002) Surface areas and ratios from elevation grid (surfgrids.avx) extension for ArcView 3.x-version 1.2. Jenness Enterprises http://www.jennessent.com/arcview/grid tools.htm
Jensen A, Frederiksen R (1992) The fauna associated with the bank-forming deep-water coral Lophelia pertusa (Scleractinaria) on the Faroe Shelf. Sarsia 77:53–69
Jonsson LG, Nilsson PG, Floruta F, Lundälv T (2004) Distributional patterns of macro-and megafauna associated with a reef of the cold-water coral Lophelia pertusa on the Swedish west coast. Mar Ecol Prog Ser 284:163–171
Kendall MS, Bauer MJ, Jeffrey CFG (2009) Influence of hard bottom morphology on fish assemblages of the continental shelf off Georgia, southeastern USA. Bull Mar Sci 84:265–286
Kissling D, Taylor G (1977) Habitat factors for reef dwelling ophiuroids in the Florida Keys. In: Taylor DL (ed) Proceedings of 3rd international coral reef symposium, vol 1. University of Miami, Miami, pp 225–231
Lessard-Pilon SA, Podowski EL, Cordes EE, Fisher CR (2010) Megafauna community composition associated with Lophelia pertusa colonies in the Gulf of Mexico. Deep-Sea Res II 57:1882–1890
Levin LA, Sibuet M, Gooday AJ, Smith CR, Vanreusel A (2010) The roles of habitat heterogeneity in generating and maintaining biodiversity on continental margins: an introduction. Mar Ecol 31:1–5
Levy A, Able KW, Grimes CB, Hood P (1988) Biology of the conger eel Conger oceanicus in the Mid-Atlantic Bight. Mar Biol 98:597–600
Luckhurst BE, Luckhurst K (1978) Analysis of the influence of the substrate variables on coral reef fish communities. Mar Biol 49:317–323
Lundblad E, Wright DJ, Miller J, Larkin EM, Rinehart R, Naar DF, Donahue BT, Anderson SM, Battista T (2006) A benthic terrain classification scheme for American Samoa. Mar Geodesy 29:89–111
Mah C, Nizinski M, Lundsten L (2010) Phylogenetic revision of the Hippasterinae (Goniasteridae; Asteroidea): systematics of deep-sea corallivores, including one new genus and three new species. Zool J Linn Soc 160:266–301
Mandelbrot BB (1983) The fractal geometry of nature. W. H. Freeman and Company, New York
McCosker JE, Ross SW (2007) A new deepwater species of the snake eel genus Ophichthus (Anguilliformes: Ophichthidae) from North Carolina. Copeia 2007:783–787
Menard A, Turgeon K, Kramer DL (2007) Selection of diurnal refuges by the nocturnal squirrelfish, Holocentrus rufus. Environ Biol Fish 82:59–70
Mortensen PB, Hovland M, Brattegard T, Farestveit R (1995) Deepwater bioherms of the scleractinian coral Lophelia pertusa (L.) at 64° N on the Norwegian Shelf: structure and associated megafauna. Sarsia 80:145–158
Mosher CV, Watling L (2009) Partners for life: a brittle star and its octocoral host. Mar Ecol Prog Ser 397:81–88
Munday PL, Jones GP, Caley MJ (1997) Habitat specialisation and the distribution and abundance of coral-dwelling gobies. Mar Ecol Prog Ser 152:227–239
Nielsen JG, Ross SW, Cohen DM (2009) Atlantic occurrence of the genus Bellottia (Teleostei, Bythitidae) with two new species from the Western North Atlantic. Zootaxa 2018:45–57
Nizinski MS (1989) Ecological distribution, demography, and behavioral observations on Periclimenes anthophilus, an atypical symbiotic cleaner shrimp. Bull Mar Sci 45:174–188
Orth RJ, Heck KL Jr, van Montfrans J (1984) Faunal communities in seagrass beds: a review of the influence of plant structure and prey characteristics on predator: prey relationships. Estuaries 7:339–350
Partyka ML, Ross SW, Quattrini AM, Sedberry GR, Birdsong TW, Potter J (2007) Southeastern United States deep-sea corals (SEADESC) initiative: a collaborative effort to characterize areas of habitat-forming deep-sea corals. NOAA Tech Memo OER 1, Silver Spring, MD
Quattrini AM, Ross SW (2006) Fishes associated with North Carolina shelf-edge hardbottoms and initial assessment of a proposed marine protected area. Bull Mar Sci 79:137–163
Roberts JM (2005) Reef-aggregating behaviour by symbiotic eunicid polychaetes from cold-water corals: do worms assemble reefs? J Mar Biol Ass UK 85:813–819
Roberts JM, Wheeler AJ, Freiwald A (2006) Reefs of the deep: the biology and geology of cold-water coral ecosystems. Science 312:543–547
Roberts JM, Henry LA, Long D, Hartley JP (2008) Cold-water coral reef frameworks, megafaunal communities and evidence for coral carbonate mounds on the Hatton Bank, north east Atlantic. Facies 54:297–316
Roberts JM, Wheeler A, Freiwald A, Cairns SD (2009) Cold-water corals: the biology and geology of deep-sea coral habitats. Cambridge University Press, UK
Robertson DR, Gaines SD (1986) Interference competition structures habitat use in a local assemblage of coral reef surgeonfishes. Ecology 67:1372–1383
Ross SW, Quattrini AM (2007) The fish fauna associated with deep coral banks off the southeastern United States. Deep-Sea Res I 54:975–1007
Ross SW, Quattrini AM (2009) Deep-sea reef fish assemblage patterns on the Blake Plateau (Western North Atlantic Ocean). Mar Ecol 30:74–92
Stevens GC (1989) The latitudinal gradient in geographical range: how so many species coexist in the tropics. Am Nat 133:240–256
Sulak KJ, Brooks RA, Luke KE, Norem AD, Randall M, Quaid AJ, Yeargin GE, Miller JM, Harden WM, Caruso JH, Ross SW (2007) Demersal fishes associated with Lophelia pertusa coral and hard-substrate biotopes on the continental slope, northern Gulf of Mexico. In: George RY, Cairns SD (eds) Conservation and adaptive management of seamount and deep-Sea coral ecosystems. University of Miami, Florida, pp 65–92
ter Braak CJF (1986) Canonical correspondence analysis: a new eigenvector technique for multivariate direct gradient analysis. Ecol 67:1167–1179
ter Braak CJF (1996) Unimodal methods to relate species to environment. Centre for Biometry Wageningen (DLO Agricultural Mathematics Group), Wageningen
ter Braak CJF, Šmilauer P (2002) CANOCO reference manual and CanoDraw for windows user’s guide: software for canonical community ordination (version 4.5). Microcomputer Power, Ithaca
Thiem Ø, Ravagnan E, Fosså JH, Berntsen J (2006) Food supply mechanisms for cold-water corals along a continental shelf edge. J Mar Syst 60:207–219
Tissot BN, Yoklavich MM, Love MS, York K, Amend M (2006) Benthic invertebrates that form habitat on deep banks off southern California, with special reference to deep-sea coral. Fish Bull 104:167–181
Weaver DC, Sedberry GR (2001) Trophic subsidies at the Charleston Bump: food web structure of reef fishes on the continental slope of the southeastern United States. Am Fish Soc Symp 25:137–152
Wilson SK (2001) Multiscale habitat associations of detrivorous blennies (Blenniidae: Salariini). Coral Reefs 20:245–251
Wilson MFJ, O’Connell B, Brown C, Guinan JC, Grehan AJ (2007) Multiscale terrain analysis of multibeam bathymetry data for habitat mapping on the continental slope. Mar Geodesy 30:3–35
Wilson SK, Burgess SC, Cheal AJ, Emslie M, Fisher R, Miller I, Polunin NVC, Sweatman HPA (2008) Habitat utilization by coral reef fish: implications for specialists versus generalists in a changing environment. J An Ecol 77:220–228
Wood J (1996) The geomorphological characterisation of digital elevation models. PhD Thesis, University of Leicester
Wood J (2005) Landserf version 2.2. http://www.landserf.org
Acknowledgments
The NOAA Undersea Research Center at UNCW provided funds (to S.W. Ross, PI) for the multibeam mapping cruise on the NOAA vessel Nancy Foster. NOAA Office of Ocean Exploration largely funded (to S.W. Ross, lead PI) submersible fieldwork and some data analyses. Environmental Defense Fund (through D.N. Rader) and NOAA Habitat Conservation Division (through Miles Croom) supplied substantial funds for this project. Friends of the NC Museum of Natural Sciences administered funds and the South Atlantic Fishery Management Council provided support. We thank the personnel of the NOAA vessel Nancy Foster, the R/V Seward Johnson and Johnson-Sea-Link submersible. We also thank M.L. Partyka, A.M. Necaise, J.P. McClain-Counts, M. Rhode, E. Cordes and A. Davies for their helpful contributions. Finally, we acknowledge support of US Geological Survey and particularly thank G.D. Brewer for facilitating this research.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by J. P. Grassle.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Quattrini, A.M., Ross, S.W., Carlson, M.C.T. et al. Megafaunal-habitat associations at a deep-sea coral mound off North Carolina, USA. Mar Biol 159, 1079–1094 (2012). https://doi.org/10.1007/s00227-012-1888-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00227-012-1888-7