Abstract
Small-scale vertical patterns of larval distribution were studied at a very nearshore larval fish assemblage, during the spring–summer period of several years, at two depth strata (surface and bottom) using sub-surface and bottom trawls. A total of 4,589 larvae (2,016 from surface samples and 2,573 from bottom samples) belonging to 62 taxa included in 22 families were collected. Most larvae belonged to coastal species. Although inter-annual variations in larval density and diversity could be found, total larval abundance was always higher near the bottom whereas diversity was higher at the surface. A marked distinction between the structure of surface and bottom assemblages was found. Sixteen taxa explained 95% of the similarity among surface samples. Larvae which contributed most to this similarity included species like clupeiformes, sparids and serranids, and also blenniids, tripterygiids and some labrids. In the bottom samples, fewer species were present, with only six taxa, almost exclusively from species which lay demersal eggs, contributing to 95% of the similarity between samples. Larvae present at the surface were significantly smaller than at the bottom. For some of the most abundant species found at the bottom, only small larvae occurred at the surface while the whole range of sizes was present at the bottom, indicating that larvae may be completing the entire pelagic phase near the adults’ habitat. These results indicate that larval retention near the reefs probably occurs for these species, although for others dispersal seems to be the prevailing mechanism.
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Introduction
Traditional sampling methods for ichthyoplankton studies are difficult to use in nearshore waters due to shallower depths, complex bottom topography (Smith et al. 1987) and wave action. This resulted in a poorer knowledge of coastal ichthyoplankton communities and their distribution patterns at small spatial scales. Several studies on nearshore larval assemblage composition and spatial distribution patterns have, however, been conducted in recent years on coral reefs (Smith et al. 1987; Kobayashi 1989; Boehlert and Mundy 1993; Leis 1993; Sponaugle and Cowen 1996; Kingsford and Finn 1997; Hendriks et al. 2001; Kingsford 2001; Wilson 2001; Sponaugle et al. 2003). In these environments, evidence is growing on the ability of larvae to actively modify their position in the water column which can result in larval retention in the vicinity of the reefs (Leis 1991a, b; Jones et al. 1999, 2005; Swearer et al. 1999, 2002; Cowen 2002; Leis and McCormick 2002; Sponaugle et al. 2002; Taylor and Hellberg 2003; Paris and Cowen 2004). Depth stratified sampling with plankton nets and light traps used in shallow waters directly over reefs (Doherty and Carleton 1997; Fisher and Bellwood 2002a; Fisher 2004; Hendriks et al. 2001; reviewed by Cowen 2002 and Leis and McCormick 2002) identified vertical distribution patterns, sometimes with a clear daily or ontogenetic basis (Leis 1986a, 1991a, b, 1993; Sponaugle and Cowen 1996; Sponaugle et al. 2003; Leis et al. 2006). In situ behavioural studies also revealed species-specific behaviours and showed that larvae of coral reef fish exhibit directional swimming capabilities and regulate their vertical position at a fine scale (Leis and Carson-Ewart 1999, 2000a; Leis and McCormick 2002; Leis 2004, 2006; Leis et al. 2006).
In temperate regions, extensive work has been done on ichthyoplankton composition and vertical distribution in oceanic or shelf waters (e.g. Kendall and Naplin 1981; Southward and Barret 1983; McGowen 1993; Moser and Smith 1993; Conway et al. 1997; Olivar and Sabatés 1997; Gray 1998; Somarakis et al. 2002; Sabatés 2004). Some studies showed evidence of vertical migration patterns for some species (for a review see Neilson and Perry 1990).
However, in nearshore waters little is known about the spatial distribution of fish larvae. Some studies on micro-scale distribution of larval fish have focused on only one species. Marliave (1981) found vertical migration patterns in Gibertidia sigalutes (Cottiidae) larvae within the first 3 m layer, in Vancouver Island. Jenkins et al. (1998, 1999) reported diurnal vertical migrations of Sillaginodes punctata (Sillaginidae) in nearshore waters. Breitburg (1989) studied in situ behaviour of Gobiosoma bosci (Gobiidae) in an oyster reef and suggested that pre-settlement schooling may be a common behaviour among temperate benthic fish species. Breitburg et al. (1995) performed field studies to examine the relationship between these aggregations and water flow and suggested that larvae actively respond to water flow patterns near reefs and that this may be determinant to understand the fine scale spatial patterns of distribution at settlement.
Differences in larval assemblages between inshore and offshore samples and, in some occasions, depth stratified patterns of distribution have been described by several authors (Boehlert et al. 1985; Cowen et al. 1993; McGowen 1993; Tilney et al. 1996; Gray and Miskiewicz 2000). Brewer and Kleppel (1986) suggested that vertical patterns of neritic fish larvae could contribute to their retention in nearshore waters. Marliave (1986) sampled the extreme nearshore over rocky reefs and found that larvae of intertidal fishes occurred more frequently along rocky shores than in adjacent sandy beaches. This author suggested that intertidal fish larvae are capable of resisting offshore and alongshore dispersal and may prefer more turbulent waters or avoid more laminar velocity gradients along sand or mud shores. Tilney et al. (1996) also suggested larval retention nearshore for some rock associated species present in the Tsitsikamma National Park Marine Reserve, South Africa.
More recently, Sabatés et al. (2003) found differences in patterns of larval distribution among species from a nearshore rocky fish assemblage in the north-west Mediterranean. Also, Vélez et al. (2005) described distinct vertical assemblages of nearshore fish larvae at Independencia Bay, Peru. These authors compared the larval composition at the surface and at 10 m depth and found that these assemblages were distinct even though a strong vertical mixing was present. However, the bottom assemblages were not sampled (the bottom at the sampling stations was at 22–25 m). For several species of this inshore assemblage, larvae were present at different developmental stages, suggesting retention in nearshore waters.
In this paper we describe the nearshore larval assemblages present at the Arrábida Marine Park (west coast of Portugal) where we have observed dense schools of larvae near the reefs at shallow depths (less than 15 m) during SCUBA diving. Our aims are (1) to investigate the composition of the coastal larval fish assemblages present during the spring–summer period; (2) to compare the structure of the assemblage and larval density at the surface and bottom depth strata; (3) to search for possible ontogenetic vertical distribution patterns.
Materials and methods
Study area
This study was carried out at the Arrábida Marine Park, between Sesimbra and Portinho da Arrábida, 30 km South of Lisbon (9°00′15″–9°03′48″W and 38°26′–38°27′N) (Fig. 1). Although located on the Portuguese west coast, the study site faces south, being protected from the prevailing north and north-west winds and waves. Relatively calm sea conditions exist throughout the year, allowing sampling in the very nearshore where wave action is negligible. Tidal currents parallel to the shoreline prevail. The nearby Sado estuary has little influence over this coastal area, given that during the spring and summer months the water flow is very reduced (Martins et al. 2002). The adjacent mountain chain of Arrábida is characterized by high vertical calcareous cliffs. Boulders of many different sizes, resulting from the disintegration of these cliffs, originate a highly heterogeneous rocky subtidal habitat where many benthic fish species occur (Gonçalves et al. 2003). In the extreme nearshore, the rocky sub-stratum extends offshore only for some tens of metres and depths are very shallow (maximum around 13 m).
Sampling procedure
Sampling was performed in the extreme nearshore (less than 50 m from shore) in the spring–summer period, when most coastal fish species breed. The surface larval assemblage was sampled in 1999 and 2000 with sub-superficial trawls, at 17 stations distributed along the study area (Table 1). The bottom assemblage was sampled at two locations in 2001 and 2002. In 2003, both depth strata were sampled (Table 1). Bottom sampling was performed along 14 days in 2001, 15 days in 2002 and 6 days in 2003, with an average of four dives per day. Surface samples were taken monthly, with a mean number of samples between nine (in 2003) and 12 (in 1999), taken in 1 or 2 days. Owing to logistic constraints, all samples were taken during the day, between 9 and 18 h, and at all tidal phases. All samples collected at each depth were considered as replicates since no longitudinal gradients in assemblage structure were found (unpublished results).
Surface samples consisted of 5-min sub-superficial (1 m depth) trawls using a standard plankton net with a 350 μm mesh size, 0.30 m mouth diameter and a mouth diameter:net length ratio of 1:5. A small 4.6 m semi-rigid inflatable boat towed the net at a distance of 20 m from the boat, and a speed of approximately 1.5 knots. Bottom sampling was performed with a plankton net attached to an underwater scooter. This net was similar to the one used at the surface trawls, but the mouth diameter:net length ratio was 1:3 due to manoeuverability reasons. The bottom plankton trawls were undertaken at a distance of approximately 0.50 m from the rocky substrate. After reaching the bottom, the diver opened the net and begun the trawl following a direction parallel to the shoreline, contouring obstacles when needed. Five minutes later the diver would close the net and slowly ascend to the surface. Sampling speed was approximately 1.5 knots. All samples were performed over the whole extent of the rocky bottom, from 4 to 13 m. In each bottom sample we followed the bottom contour at approximately the same depth. The average difference between maximum and minimum depths per bottom sample was 1.92 m (SD = 0.86). Hydrobios flowmeters were attached to both nets. Filtered volumes, sampling periods and number of larvae caught are shown in Table 1.
All samples were preserved in 4% saline formalin buffered with sodium borate for at least 1 month, before larvae were sorted and identified under a stereomicroscope to the lowest possible taxonomic level (species level when possible). We identified 94% of the larvae to family level (99% in the bottom samples and 88% in the surface samples), 86% to genus level (97% in the bottom samples and 71% in the surface samples) and 83% to species level (95% in the bottom samples and 69% in the surface samples).
Photographs were taken to help in the identifications, using a digital camera attached to a stereomicroscope. Body length (BL), corresponding to notochord length in pre-flexion larvae or to standard length in post-flexion larvae, was measured to the nearest 0.01 mm using a micrometer scale. For larvae larger than 15.0 mm measurements were made using a caliper. A total of 14.5% of larvae in the surface samples and 5.1% in the bottom samples were in bad condition and were not measured.
Data analysis
Composition and annual patterns of larval assemblages
Larval abundances were calculated for every taxa identified in each sample and are expressed as the number of larvae/1,000 m3. Two biodiversity indices were calculated for each sample, the Shannon diversity index (H′) using the natural logarithm in its formulation and the average taxonomic distinctness index (Delta*), which reflects the taxonomic spread of species among samples (Clarke and Warwick 2001). This index is based not just on the species abundances but also on the taxonomic distances between every pair of individuals; high Delta* values (maximum = 100) reflect high taxonomic diversity in the assemblage (Clarke and Warwick 2001). Equal step-lengths were assumed between each taxonomic level. Four taxonomic levels were used, from species to order. Mean values and standard deviation of these indexes were calculated for each year at each depth strata.
Annual differences in total larval abundances and diversity indexes were tested with one-way ANOVA and Student-Newman–Keuls tests for post hoc comparisons, when homoscedascity assumptions were met after being tested with the Levene’s test. If needed, variables were log (x + 1) transformed. When variances were heterogeneous, a Kruskall–Wallis ANOVA was used and post hoc comparisons were performed with the Dunn’s test. Using the same criteria, T-student tests or Mann–Whitney U tests were used for the comparisons of overall abundance and diversity indexes between surface and bottom samples.
Owing to a possible effect of tide on larval distribution (Neilson and Perry 1990; Cowen 2002) and logistical constraints (it was not possible to standardize tide situation), tidal phase was randomized in this study. However, we tested for a possible interaction between tide and depth on larval abundance using a factorial ANOVA, with tidal phase and depth strata as factors. Since no interaction between these factors was found, but, when pooled together, homoscedascity assumptions could not be met even after transformation (due to the great difference in variance between surface and bottom samples), we analysed these factors separately with one-way ANOVA and Student-Newman–Keuls tests for post hoc comparisons.
Differences in larval assemblages between depth strata
Using the relative abundance of each species, differences between the structure of surface and bottom assemblages were graphically displayed with a non-metric multidimensional scaling (MDS) two-dimensional plot. The ordination was based on a triangular matrix of Bray–Curtis similarities after a log (x + 1) data transformation. Samples in plots that are closer together are less distinct and a stress coefficient determines the relationship among samples from distinct groups (Clarke and Warwick 2001). Larvae which could not be identified were not considered in the analysis: 11.1% of the larvae present in surface samples (from which 87% were in the pre-flexion stage) and 0.58% of the larvae from the bottom samples (98% of which were in the pre-flexion stage). Six groups were considered in the analysis, corresponding to the different years sampled at each depth.
In order to test for differences between groups a one-way analysis of similarities (ANOSIM) was performed. High R values indicate differences between groups (Clarke and Warwick 2001). Similarity percentages analysis (SIMPER) was used to determine the species contribution to each group after log (x + 1) transformation of the data, assuming a cut-off at 95%. The MDS stress level was higher than 0.1 (Clarke and Warwick 2001), and therefore we performed a cluster analysis based on the Bray–Curtis similarities matrix with log (x + 1) transformed data. To simplify the cluster graphical interpretation, we used the average similarity contribution of each species to the average similarity within each year at each depth, according to the SIMPER results. In order to understand the species composition at each depth, since low R values were obtained in every pair-wise comparison between years in the same depth strata, inter-annual results were pooled together for the same depth before the SIMPER analysis.
Ontogenetic vertical distribution patterns
To access possible ontogenetic differences in the distribution of larvae between depth strata, the length of larvae of the most representative species was compared between the surface and bottom samples with T-student tests (the log x transformation was used when needed) or Mann–Whitney U tests (if variances were heterogeneous even after transformation). Developmental stage of each larva was categorized into pre-flexion, incomplete flexion and post-flexion stages following Leis and Carson-Ewart (2000b). We considered all larval stages from hatching, including yolk-sac larvae.
The PRIMER 5 programme was used for the calculation of diversity indexes and multivariate analyses. STATISTICA 7 (StatSoft, Inc. 2004) was used for all other statistics.
Results
Composition and annual patterns of larval assemblages
A total of 4,589 larvae (2,016 from the surface samples and 2,573 from the bottom samples) were collected belonging to 62 identifiable taxa included in 22 families (Table 2). Most larvae caught belonged to species whose adults live in nearshore waters laying demersal eggs (e.g. Blenniidae, Gobiidae, Tripterygiidae, some Labridae). However, there were also a few coastal larvae hatching from pelagic eggs (e.g. Sparidae, Serranidae) and species whose adults live and spawn in coastal and shelf waters like Sardina pilchardus, Trachurus trachurus and Engraulis encrasicolus.
Although variation in total larval abundance among years was apparent for both depth strata (Fig. 2), total larval abundance was always higher at the bottom than at the surface and overall differences were significant (Z = 6.214, P < 0.001). The inter-annual variation in larval density in the bottom samples was not significantly different (H = 4.26, df = 2, P = 0.12), but at the surface significant variations between years were found (F = 3.673, df = 2, P < 0.05), with larval densities observed in 2000 lower than both in 1999 (P < 0.05) and 2003 (P < 0.05). No differences of larval abundance were found among tidal phases at the surface (F = 0.46, df = 3, P = 0.71), but significant results were obtained at the bottom (F = 4.81, df = 3, P < 0.01) with more larvae occurring at low tide than at rising tide (P < 0.05).
Diversity was significantly higher in the surface samples (Table 3). Annual variation in diversity was significant at the bottom samples for both the Shannon diversity index and the average taxonomic distinctness index, with a decrease in the overall diversity in 2003 and an increase in taxonomic diversity in 2002. On the contrary, no significant changes in taxonomic diversity were found in the surface samples, but overall diversity exhibited a significant decrease in 2000 (Table 3).
Differences in larval assemblages between depth strata
The MDS graphical representation showed a clear distinction between the structure of surface and bottom assemblages (Fig. 3a), which was confirmed by the cluster analysis (Fig. 3b). ANOSIM revealed that these differences were significant (Global R = 0.46, P = 0.001; Table 4). There were low R values in every pair-wise comparison between years in the same depth strata, but all comparisons between any surface layer group with any bottom layer group revealed high values of R (above 0.50), showing significant differences between the structure of surface and bottom assemblages (Clarke and Warwick 2001).
The similarity percentages analysis (SIMPER) showed that surface assemblages included 16 taxa from which only six taxa explained 73.4% of the similarity among groups (Table 5). Larvae that contributed most to this similarity include sparids, serranids (Serranus sp.), blenniids (Parablennius pilicornis), clupeiformes (Sardina pilchardus), labrids (Coris julis) and tripterygiids (Tripterygion delaisi). In the bottom samples only six taxa were present, almost exclusively from coastal species which lay demersal eggs. The only exception was the sparid Boops boops which lays pelagic eggs but also breeds in nearshore waters. Gobiids dominated this assemblage with only two species, Pomatoschistus pictus and Gobius xanthocephalus, explaining together 73.79% of the similarity among groups (Table 5).
Ontogenetic vertical distribution patterns
Larvae present at the surface were significantly smaller than at the bottom (surface: mean = 2.99 mm, SD = 1.39, range 1.07–17.07, N = 1,724; bottom: mean = 7.24 mm, SD = 2.36, range 1.10–23.00, N = 2,442; Z = 48.62, P < 0.001). This overall pattern was found for most species present at the bottom with the exceptions of Callionymus spp., Sparidae sp.1 and Tripterygion delaisi (Table 6). Most larvae caught at the surface were small and undeveloped (83.0% of the larvae were less than 4 mm BL and 92.3% were in the pre-flexion stage). On the contrary, larvae caught at the bottom were larger (94.8% > 4 mm, see Fig. 4) and more developed (90.3% were in the flexion or post-flexion stages).
During the analysis of the size distribution of the most representative species (according to the SIMPER analysis), an interesting pattern emerged for those species which were present at both depths. In most cases, only small larvae occurred at the surface whereas all size classes were present at the bottom (Fig. 5). For species which were abundant at the bottom, larvae from 4 mm to the 10–12 mm or to the 18–20 mm size classes (depending on the species considered) were present. These patterns of small larvae at the surface and different size classes at the bottom could be observed in the gobiids Pomatoschistus pictus, Gobiusculus flavescens and Gobius niger; the sparid Boops boops and in labrids from the genus Symphodus. Symphodus melops larvae constituted 55.6% of the Symphodus larvae present at the bottom where they were present in the different size classes. Small Symphodus larvae (2–4 mm size class) present at the surface and included in the category Symphodus spp., may belong to either S. melops or Symphodus cinereus, since adults of both species are common at the study site and both larvae have similar pigmentation patterns when newly hatched (Quignard 1967, 1968; Fives 1976). Finally, Tripterygion delaisi, the third most abundant species at the bottom, represents an exception to this pattern, with an overlap of size-class distribution for larvae caught at the surface and at the bottom with slightly bigger larvae present at the surface (Fig. 5).
Discussion and conclusions
The very nearshore larval fish assemblages studied in the present work were exclusively composed by shore or shelf-dwelling species. Larvae from shore fish species included sparids, serranids, blenniids, gobiids, tripterygiids and labrids, reflecting the adult fish assemblage occurring at the study area (Henriques et al. 1999). Larvae from shelf-dwelling spawners were mainly clupeids, carangids and engraulids.
These results generally agree with Sabatés et al. (2003) who found nearshore larval assemblages at a rocky shore in the northwest Mediterranean to be essentially composed by shorefish species (also including gobiids, sparids, labrids, tripterygiids and a few shelf species). Other studies found similar results in other geographic areas: New Zealand (Kingsford and Choat 1989); Gulf of California (Brogan 1994); South Africa (Tilney et al. 1996); Peru (Velez et al. 2005). Larvae from slope or oceanic families that are abundant off the Portuguese coast, like myctophids or paralepidids (John and Ré 1993), were not found.
Most coastal species known to breed at the Arrábida Marine Park during the spring and summer period (Henriques et al. 1999) were present in our samples. However, there were a few exceptions, like clingfishes (family Gobiesocidae). Some authors have shown that clingfish species can be abundant near reefs (e.g. Marliave 1986; Kingsford and Choat 1989; Tilney et al. 1996; Sabatés et al. 2003). Using light-traps we have been able to confirm this as we caught many clingfish larvae from all size classes in the study area (unpublished results). A possible explanation for the fact that, although clingfish larvae are present in the area they were not collected in our samples, could be related to the short planktonic larval duration (PLD) of these fishes (15 days for Apletodon dentatus and 13 days for Lepadogaster candolii; Raventós and Macpherson 2001). The fast development of ontogenetic structures, which could contribute to an active behaviour of net avoidance, is probably associated with these short PLDs. In fact, these species hatch at a large size with an advanced stage of development and are probably able to actively swim and find shelter (e.g. hiding among algal tufts, Gonçalves et al. 2003), thus being able to avoid the net very early in life. This could explain why they were caught using light-traps, but absent from the bottom and surface sampling using nets.
Larval assemblages at the surface and at the bottom were clearly distinct, indicating that this very nearshore larval fish assemblage is vertically structured at a small scale (a few metres). The surface assemblage was much more diverse, being composed by coastal larvae hatching from both pelagic and demersal eggs. Although filtered volumes were also higher at the surface (due to the different net diameter: net length ratios of the nets), similarity was higher among bottom samples. This fact, together with the larger size of larvae collected in the bottom samples (larger larvae could be expected to more easily avoid the net actively than smaller larvae), seems to indicate that the detected differences in diversity were not due to the different filtered volumes between surface and bottom samples. The bottom assemblage was composed by a small number of exclusively nearshore reef-associated species laying demersal eggs (like gobiids, tripterygiids and labrids of the genus Symphodus). The exception was the sparid Boops boops, which is abundant in the study area and also breeds nearshore (Henriques et al. 1999), but spawns pelagic eggs.
At the surface, inter-annual fluctuations were detected, with the year 2000 presenting significant lower larval densities and diversities. These variations could be related to the inter-annual fluctuations of the North Atlantic Oscillation (NAO), since, coincidently, the year 2000 presented particularly high winter NAO Index values.Footnote 1 However, the pattern of variation was different at the bottom. Therefore, more data are needed to further analyse this relation, although it is possible that inter-annual differences could be caused by more general climatic fluctuations. This relation has been recently described for this study area concerning the adult rocky fish assemblage (Henriques et al. 2006). Despite these inter-annual fluctuations, overall larval density was always much higher at the bottom than at the surface.
Although tidal effects were not specifically addressed in this study and tidal phase was randomized in our sampling design, a preliminary analysis showed that samples collected in the bottom at low tide contained significantly higher larval abundances than at the rising tide. In future work, this possible tidal effect should be explored in order to evaluate if these patterns are consistent and determine if they are a simple concentration effect of larvae in a small water column at low tide and/or if they reflect any behavioural mechanism which allows these larvae to be retained nearshore. The fact that surface and bottom patterns were strikingly different indicates that larvae must have an active role in the observed patterns.
One could argue that the described vertical patterns occurred during the day and that they could be different at night, since it is well established that nocturnal ascent of larger larvae is one of the commonest patterns of diel vertical migration of coastal larval fish (Leis 1991a; Fisher 2004). However, night trawling at the surface in the same period of the year showed that, for the species considered, the patterns found during the day are maintained at night, with only small larvae found in sub-surface trawls (unpublished results).
The high diversity values found at the surface and the high density values found at the bottom indicate that, for a selected number of species, larvae school near the substrate at high densities. The gobies Pomatoschistus pictus and Gobius xanthocephalus dominated this assemblage. Several studies have documented the presence of Gobiidae larvae nearshore (Leis 1986a, 1993; Smith et al. 1987; Kingsford and Choat 1989; Kobayashi 1989; Gray 1993; Brogan 1994; Gray and Miskiewicz 2000; Kingsford 2001; Sabatés et al. 2003; Sponaugle et al. 2003), but little is known about the small-scale distribution patterns near the substrate in very nearshore waters. Moreover, some gobies are present nearshore at all size classes of their planktonic life in different environments. Leis et al. (1998) found this pattern in gobies occurring in shallow waters at Taiaro atoll and concluded that they completed their entire planktonic life cycle near the reefs. The same result was obtained by Leis et al. (2003) for several fish families (including the Gobiidae) in four lagoons at two atolls and one island in the French Polynesia. In temperate waters, Beyst et al. (1999) sampled the hyperbenthos at a maximum depth of 10 m, in subtidal and tidal marshes at the Dutch Delta, and found Pomatoschistus microps and Pomatoschistus lozanoi larvae within the full range of developmental sizes (3–20 mm). Drake and Arias (1991) sampled larvae in a shallow coastal inlet at south-west Spain and described that P. microps was the most abundant species with larvae ranging from 5 to 13 mm while Gobius paganellus ranged from 7 to 13 mm. Brogan (1994) also found larvae of reef-associated species to be present in all size classes near reefs at the Gulf of California.
The larvae from the surface assemblage were mostly small and undeveloped. This indicates that these larvae are essentially newly hatched, which is in accordance with the presence of spawning grounds for most of these species in the study area. The absence of bigger larvae could be indicative of net avoidance by more developed larvae at the surface, given the small size of the net. However, more developed larvae were caught near the bottom with a similar net. On the other hand, the light intensity is not much attenuated near the shallow bottom and therefore the ability of larvae to visually avoid the net is probably similar at both depths. Furthermore, samples collected at night also contained mostly less developed larvae (own unpublished results).
For some of the most abundant species occurring at the bottom, our results provide evidence of depth-related ontogenetic distribution patterns, with smaller larvae, mostly newly hatched, at the surface and larger and more developed larvae at the bottom. This is true for Pomatoschistus pictus, Gobiusculus flavescens, Gobius niger, Boops boops and probably for Symphodus melops. Moreover, larvae of these species were found at the bottom in the whole size range of their planktonic phase. Size at settlement varies with the species considered: around 17–18 mm for P. pictus; 12 mm for G. flavescens; and 9 mm for G. niger (Petersen 1919; Russell 1976). In Gobius xanthocephalus, size at settlement is unknown, but larvae were present in the bottom samples at up to the 14–16 mm size class. This indicates that this species is, most likely, also completing its planktonic life nearshore. In the case of B. boops, larvae settle within 16–18 days at a TL of 12 mm (Raventós and Macpherson 2001).
The vertical distribution of fish larvae may influence larval dispersal (Sponaugle et al. 2002; Paris and Cowen 2004; Leis 2006); in particular, remaining near the bottom where flow is reduced may favour larval retention near reefs (Leis 1986b; Steffe 1990; Breitburg 1989, 1991; Breitburg et al. 1995). Retention of larvae near reefs has been documented in recent years in different systems and is presently identified as an important mechanism of self-recruitment for some coral reef populations (e.g. Jones et al. 1999, 2005; Swearer et al. 1999, 2002; Sponaugle et al. 2002; Taylor and Hellberg 2003; Paris and Cowen 2004). One of the advantages of nearshore retention for coastal species is the ability to find a suitable habitat to settle. Dispersion may increase mortality since oceanographic processes influencing larval transport are variable, both temporally and spatially, and if larvae are not transported to an adequate habitat, they can be lost (Hickford and Schiel 2003). Although the length of larval life has been proposed as one of the primary determinants of dispersal ability (Thresher et al. 1989; Sponaugle et al. 2002; Lester and Ruttenberg 2005), this relationship is not universal (see Lester and Ruttenberg 2005). Nonetheless, larvae with a small PLD would have more difficulty in returning to coastal habitats after pelagic dispersal in the ocean and in choosing the right habitat to settle. The data available on PLDs for some of the main species found at our study site show that, for Boops boops and Symphodus melops, PLD is less than 19 days (Raventós and Macpherson 2001). For Pomatoschistus pictus and Gobius xanthocephalus, PLDs are not known, but for other gobies which occur at our study area somewhat longer times have been described (25 and 22 days for Gobius paganellus and Gobius cobitis, respectively; Gil et al. 1997; Borges et al. 2003).
When compared with larvae hatching from pelagic eggs, shorefish larvae hatching from demersal eggs are larger and typically have functional eyes, developed fins and guts, and better swimming abilities (Thresher 1984; Hickford and Schiel 2003). Therefore, retention is more likely to occur in these kinds of larvae (Sponaugle et al. 2002). However, larvae from some of these species seem to disperse. For instance, for the most abundant blenny at our site, Parablennius pilicornis, small larvae were very abundant in surface samples but almost no larvae were caught at the bottom. Drake and Arias (1991) also found only small Parablennius sp. larvae (3–5 mm) inshore. Some authors have suggested that blenniids disperse away from reefs (Brogan 1994). The long PLD (over 70 days at controlled conditions, personal communication by C. Faria) and well-developed pectoral fins of P. pilicornis larvae make them good candidates for dispersal. Larvae of this species are often found offshore in the upper layers of the water column (Olivar 1990).
Our results seem to indicate that, although PLD, size and development characteristics at hatching can be important in determining the larval capability to remain near the adults’ habitat in these coastal species, other factors must be involved. Larval swimming and sensory abilities and orientation capabilities may also have a strong impact in dispersal patterns as larvae can actively influence their position in the water column (Leis and Carson-Ewart 1999, 2000a; Victor and Wellington 2000; Montgomery et al 2001; Cowen 2002; Fisher and Bellwood 2002b; Leis 2002, 2006; Leis and McCormick 2002; Mora and Sale 2002; Myrberg and Fuiman 2002; Fisher and Wilson 2004; Fisher 2005).
The data presented herein indicate that, for some of the most abundant coastal fish species that occurred at our study site, larvae can complete their entire planktonic phase in the vicinity of the adults’ habitats. Moreover, they seem to be able to actively choose bottom habitats very early in their pelagic phase and not just in the pre-settlement stage, as is most commonly reported (Leis 2006). For many of these species, larvae are probably able to remain near the bottom, as soon as their swimming and sensory abilities develop. The observed vertical distribution patterns, combined with other factors, could influence horizontal positioning, promoting retention near the benthic rocky habitats at the study area. Larval distribution patterns depend on the interaction between physical oceanographic features and biological factors, like the adults’ behaviour and ecology, life history traits, PLD, larval behaviour and sensory capabilities (Cowen 2002; Leis 2002, 2006; Sponaugle et al. 2002), and are most likely species-specific. The extent to which the results presented in this paper could influence self-recruitment in these assemblages needs further investigation. For instance, the relationship often found in temperate waters between larval dispersal patterns and mode of spawning can in fact be stronger in sheltered coasts (Hickford and Schiel 2003). Although this relationship was not found in our study, the degree of larval retention near reefs can be favoured by the sheltered conditions of the Arrábida Marine Park shore. It is therefore possible that the vertical distribution patterns found in this study differ, for some species, from those found in more exposed shores (Leis 2006). Studies focusing on the active behaviour of larvae and their sensory and swimming abilities as well as comparisons of larval distributions for the same species in more exposed shores may further contribute to understand the very nearshore distribution patterns described in this paper.
Notes
See NAO Index data provided by the Climate Analysis Section, NCAR, Boulder, USA, Hurrell (1995) at http://www.cgd.ucar.edu/cas/jhurrell.
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Acknowledgments
This study was supported by the Portuguese Science and Technology Foundation [Fundação para a Ciência e a Tecnologia (FCT)] as part of the project POCTI/BSE/38350/2001 and through the Pluriannual Program (R&D Unit 331/94). FCT also supported the PhD grants of R. Borges (SFRH/BD/4852/2001) and R. Beldade (SFRH/BD/1013/2000). We would like to thank A. Chícharo, R. Ben-Hamadou and J. Maroco for their help with the statistical analysis, M. Barbosa, A. Catarino, J. Martins, N. Souza and S. Vizinho for assistance in the field and lab work and the Oceanographic Museum of Parque Natural da Arrábida for all the support. Sampling and laboratory procedures comply with current Portuguese legislation.
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Borges, R., Beldade, R. & Gonçalves, E.J. Vertical structure of very nearshore larval fish assemblages in a temperate rocky coast. Mar Biol 151, 1349–1363 (2007). https://doi.org/10.1007/s00227-006-0574-z
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DOI: https://doi.org/10.1007/s00227-006-0574-z