Abstract
A biosensor based on stearic acid-graphite powder modified with sweet potato (Ipomoea batatas (L.) Lam.) tissue as peroxidase source was constructed and applied in organic solvents. Several parameters were studied to evaluate the performance of this biosensor such as stearic acid-graphite powder and tissue composition, type and concentration of supporting electrolyte, organic solvents, water/organic solvent ratio (% v/v) and hydrogen peroxide concentration. After selection of the best conditions, the biosensor was applied for the determination of hydroquinone in cosmetic creams in methanol. At the peroxidase electrode hydroquinone is oxidized in the presence of hydrogen peroxide and the radical formed was reduced back electrochemically at –180 mV vs Ag/AgCl (3.0 mol L–1 KCl). The reduction current obtained was proportional to the concentration of hydroquinone from 6.2 × 10–5 to 1.5 × 10–3 mol L–1 (r = 0.9990) with a detection limit of 8.5 × 10–6 mol L–1. The recovery of hydroquinone from two samples ranged from 98.8 to 104.1% and an RSD lower than 1.0% for a solution containing ¶7.3 × 10–4 mol L–1 hydroquinone and 1.0 × 10–3 mol L–1 hydrogen peroxide in 0.10 mol L–1 tetrabutylammonium bromide methanol-phosphate buffer solution (95:5% v/v) (n = 10) was obtained.
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Received: 13 January 2000 / Revised: 10 April 2000 / Accepted: 18 April 2000
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Fatibello-Filho, O., Cruz Vieira, I. Construction and analytical application of a biosensor based on stearic acid-graphite powder modified with sweet potato tissue in organic solvents. Fresenius J Anal Chem 368, 338–343 (2000). https://doi.org/10.1007/s002160000467
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DOI: https://doi.org/10.1007/s002160000467