Abstract
The operation of mitogen-activated protein kinase (MAPK) signal transduction pathways is one of the most important mechanisms for the transfer of extracellular information into the cell. These pathways are highly conserved in eukaryotic organisms. In fungi, MAPK pathways are involved in the regulation of a number of cellular processes such as metabolism, homeostasis, pathogenesis and cell differentiation and morphogenesis. Considering the importance of pathways, in the present work we proceeded to identify all the genes that are regulated by the signal transduction pathway involved in mating, pathogenesis and morphogenesis of Ustilago maydis. Accordingly we made a comparison between the transcriptomes from a wild-type strain and an Ubc2 mutant affected in the interacting protein of this pathway by use of microarrays. By this methodology, we identified 939 genes regulated directly or indirectly by the MAPK pathway. Of them, 432 were positively, and 507 were negatively found regulated. By functional grouping, genes encoding cyclin-dependent kinases, transcription factors, proteins involved in signal transduction, in synthesis of wall and cell membrane, and involved in dimorphism were identified as differentially regulated. These data reveal the importance of these global studies, and the large (and unsuspected) number of functions of the fungus under the control of this MAPK, providing clues to the possible mechanisms involved.
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Introduction
Signal transduction pathways are involved in the transfer of information from the exterior to the nucleus in all eukaryotic cells. Among them, we may cite the Mitogen-activated protein kinase (MAPK) signaling pathway that has important roles in several cellular processes such as transformation, proliferation, differentiation, development and apoptosis (reviewed by Zhang and Liu 2002). In lower eukaryotic organisms such as fungi, MAPK pathways are rather conserved, and among their functions, there are the regulations of gene expression during cellular responses, allowing the adaptation of the organisms to different environmental conditions. In these organisms, up to five different MAPK pathways have been described to be involved in different cellular processes; for example in Saccharomyces cerevisiae, MAPK pathway responding to different cues have been described: pheromone response, filamentous growth, high osmolarity, cell wall integrity and spore wall assembly (Gustin et al. 1998; Pan et al. 2000; Gancedo 2001; Palecek et al. 2002; Chen and Thorner 2007).
The MAPK pathways involve the operation of three protein kinases in hierarchical order: MAPKKK (MAP kinase kinase kinase), MAPKK (MAP kinase kinase) and MAPK (MAP kinase), which are sequentially activated by phosphorylation at specific sites. Normally these protein kinases become associated through an adaptor protein. The activation process initiates by the perception of a signal by a transmembrane receptor and the action normally of heterotrimeric G proteins that transfer the signal to MAPKKK that starts the cascade of phosphorylation reactions. Finally, the MAPK (MAP kinase) phosphorylates distinct transcription factors that regulate gene expression in response to the sensed stimuli (Brefort et al. 2009; Ruiz-Herrera et al. 2009; Raudaskoski and Kothe 2010; Vollmeister et al. 2011; Ruiz-Herrera and Campos-Góngora 2012).
Contrasting to other fungi, in U. maydis it has been described the existence of only two MAPK pathways. The best one characterized is involved in mating, pathogenesis and dimorphism (Brefort et al. 2009; Vollmeister et al. 2011). A second MAPK pathway involved in cell wall integrity (CWI) was described further on (Carbó and Pérez-Martín 2010), and the possible existence of a third one that would be involved in response to osmotic stress has been entertained (Perez-Nadales et al. 2014).
Previously, we isolated a mutant of U. maydis (CL211) that is unable to carry out the yeast-to-mycelium dimorphic transition, grows constitutively in the yeast-like form, and is non-virulent to maize (Martínez-Espinoza et al. 1997). A genetic analysis of the mutant demonstrated that it is affected in UBC2 (Ustilago bypass of cyclase; Mayorga and Gold, 2001) gene encoding the MAP kinase adaptor protein belonging to the best known MAPK pathway (Martínez-Espinoza et al. 2004) and that for facility, we refer to as PMM (pathogenesis, mating and morphogenesis).
U. maydis is a dimorphic Basidiomycota fungus and an attractive model organism for the analysis of different cellular processes (Bölker 2001; Brefort et al. 2009; Ruiz-Herrera et al. 2009; Vollmeister et al. 2011; Valdés-Santiago et al. 2012; Ruiz-Herrera and Campos-Góngora 2012, Ruiz-Herrera et al. 2013; Valdés-Santiago and Ruiz-Herrera 2014; León-Ramirez et al. 2014). In nature U. maydis infects maize (Zea mays L.) and teozintle (Z. mays subsp. parviglumis), where it completes its sexual life cycle. Under axenic conditions, the fungus is able to infect plant species phylogenetically unrelated to maize (León-Ramírez et al. 2004; Méndez-Morán et al. 2005, Martínez-Soto et al. 2013; Ruiz-Herrera et al. 2013). In addition, when incubated under defined environmental in vitro conditions, U. maydis performs a completely different sexual life cycle with the formation of basidiocarps (Cabrera-Ponce et al. 2012).
Considering the importance of MAPK pathways in the regulation of metabolism in fungi that there is almost no knowledge of the whole set of genes whose transcription is regulated by the MAPK pathways in U. maydis and in other fungi, and that the approaches to understand their operation have relied mostly on reverse genetics and biochemical techniques (Banuett and Herskowitz 1994; Mayorga and Gold 1999; Müller et al. 1999; Andrews et al. 2000; Müller et al. 2003; Martínez-Espinoza et al. 2004), we have proceeded to make an analysis of all the U. maydis genes regulated by the PMM MAPK pathway, making use of the CL211 ubc2 mutant affected in this system.
Materials and methods
Ustilago maydis strains and culture conditions
In this work, we used the wild-type strain FB2 (a 2 b 2 ; Banuett and Herskowitz 1989) and the CL211 strain (a 2 b 2 , ubc2; Martínez-Espinoza et al. 1997, 2004), a constitutive monomorphic yeast mutant deficient in the gene encoding the MAP kinase pathway-interacting protein UBC2 (Martínez-Espinoza et al. 1997, 2004; Mayorga and Gold 2001). The strains were maintained in 50 % glycerol in complete medium (MC; Holliday 1974) at −70 °C, and recovered in MC liquid medium. Growth in minimal medium (MM; Holliday 1974) took place at pH 7. Under these conditions, the wild-type strain and the mutant grow yeast-like (Ruiz-Herrera et al. 1995; Martínez-Espinoza et al. 1997).
Isolation of RNA and microarrays hybridization
Similar conditions to those described by Martínez-Soto and Ruiz-Herrera (2013) were used. Three independent cultures (biological replicates) of U. maydis strains (106 cells/ml) were grown in MM pH 7 at 28 °C under shaking conditions for 16 h. The cells were recovered by centrifugation, and RNA was isolated using Trizol (Invitrogen, Carlsbad, CA, USA) and purified with QIAGEN (Hilden, Germany) columns. RNA concentration was measured by absorbance at 260 nm with a Nanodrop (Termo Scientific, Waltham, MA, USA), and its integrity was determined by electrophoresis in denaturing agarose gels. The RNA samples coming from the three independent cultures were mixed together, and used for synthesis and subsequent labeling of cDNA, and microarray hybridization. These two last procedures were performed by Roche NimbleGen Inc. (Reykjavík, Iceland).
Microarray analysis
The type of microarrays used in this work were the same as previously described (Martínez-Soto and Ruiz-Herrera 2013) from NimbleGen, characterized by having five different oligonucleotides of 60 nt in length designed along the full gene length in duplicate, according to a design from Scott Gold (University of Georgia). It must be stressed that these conditions secure that data for each one of the 6,883 U. maydis genes represents an average of ten determinations. Scan and normalization of the data were done as described previously (Martínez-Soto and Ruiz-Herrera 2013). ArrayStar software from DNAStar was used for microarray analyses, P values were adjusted by the false discovery rate (FDR) method (Benjamini and Hochberg 1995) and P values <0.05 were considered differentially expressed. Genes whose expression values were higher (up-regulated) in the wild-type strain were considered to be positively regulated by the MAPK pathway, and those whose expression was higher in the mutant (down-regulated) were considered to be negatively regulated by the pathway. A value of twofold change up or down was considered the cutoff to determine whether a gene was positively or negatively regulated.
Functional grouping and search for specific genes
The functional annotation of the total regulated genes by the PMM MAPK pathway in U. maydis was performed with the Functional Catalogue (FunCat) online program (Ruepp et al. 2004), and with the aid of R statistical software; and supported by the MIPS Ustilago maydis Database (http://mips.helmholtz-muenchen.de/genre/proj/ustilago/), and the Ustilago maydis Database-Broad Institute (http://www.broadinstitute.org/annotation/genome/ustilago_maydis). The search of genes previously described and related to U. maydis dimorphism was made based on the data previously reported by Heimel et al. (2010) and Martínez-Soto and Ruiz-Herrera (2013). For handling of data sets, Venn diagrams were used (http://www.bioinformatics.lu/venn.php).
Search of domains in genes differentially regulated as clusters
For the identification of possible domains, and the presence of a signal peptide in proteins encoded by genes described as Unclassified, which are present in clusters, their amino acid sequences were analyzed with the Pfam (Punta et al. 2012), SMART (Letunic et al. 2012), and SignalP (Bendtsen et al. 2004) online programs; and supported by NCBI database (http://www.ncbi.nlm.nih.gov/).
Search of consensus sequences and binding sites for transcription factors
To identify consensus sequences for transcription factors in genes encoding cyclin-dependent kinases, protein kinases, serine/threonine protein kinases, and genes grouped in clusters, 1,000 base pairs upstream of the ATG site were analyzed using the JASPAR online program (http://jaspar.binf.ku.dk/) based on the S. cerevisiae genome. BLAST of NCBI page online (http://blast.ncbi.nlm.nih.gov/Blast.cgi), MIPS Ustilago maydis Database and Ustilago maydis Database-Broad Institute were used to identify U. maydis homologue genes for the transcription factors identified.
Results and discussion
To identify the genes regulated by the PMM MAPK pathway in U. maydis, we proceeded to make a comparison of the transcriptomes of the wild-type strain FB2 and the CL211 ubc2 mutant, both grown at pH 7. Under these conditions, the morphology of both strains is yeast-like, and the only variable involved is the mutation in the UBC2 gene. The results obtained revealed that of total 6,883 genes of U. maydis, 939 genes were differentially expressed between the two strains. Of these, 432 (46.0 %) were up-regulated and 507 (54.0 %) were down-regulated in the wild type (see Table S1), indicating that the former are positively regulated and the latter negatively regulated by the PMM MAPK pathway.
Functional grouping of the differentially regulated genes is shown in Fig. 1. The categories with the higher number of differentially expressed genes were Unclassified Proteins with 362 genes (38.6 %), and Metabolism and Energy with 202 genes (21.5 %). Genes grouped in this latter category were mainly related to general metabolism and metabolism of carbohydrates, amino acids and lipids; genes of the second one generally were found repressed, and those from the third one were generally overexpressed. On the other hand, 54 genes (5.7 %) were grouped into the Synthesis and Protein Fate category, and most of them were negatively regulated by PMM MAPK pathway (see Table S1). Six categories with a smaller number of differential genes showed relevance to the metabolic pathway analyzed here. These were the following: (1) Cell cycle and DNA Processing, with 38 differential genes (4.0 %); (2) Transcription, with 42 differential genes (4.5 %); (3) Cellular Transport, with 104 differential genes (11.1 %); (4) Signal Transduction Mechanism, with 41 differential genes (4.4 %); (5) Biogenesis of Cellular Components, with 50 differential genes (5.3 %); and (6) Differentiation and Cell Fate, with 21 differential genes (2.2 %). In the category of Cell Cycle and DNA Processing, a number of genes encoding cyclin-dependent kinases (cdk), cell-division cycle proteins (CDC), and other genes involved in cell cycle were found positively regulated by the PMM MAPK pathway (Table 1). For example, genes um03992 encoding Dip1–Don3 interacting protein, um03234 encoding Cdc5-serine/threonine-protein kinase, um10499 related to Hos4-subunit of the Set3 complex and um00277 encoding an M-phase inducer phosphatase were 2.8-, 2.9-, 3.0- and 4.6-fold up-regulated, respectively. DIP1 gene has been previously mutated in U. maydis, and the mutant strain was not affected in cytokinesis; but the double mutation in DIP1 and DON3 genes affected nuclear separation during mitosis. Moreover, DON3 has been associated with the formation of secondary septa in U. maydis (Sandrock et al. 2006). CDC5 gene has been reported in Schizosaccharomyces pombe as a cell cycle regulator that acts on the G2/M transition (Bernstein and Coughlin 1997). Also this gene has been described as necessary for initiation of DNA replication in S. cerevisiae (Kitada et al. 1993). The Set complex is a multiprotein complex involved in the mitotic cell cycle, as well as in sporulation in yeasts such as S. cerevisiae (Pijnappel et al. 2001). And the last gene, um00277, has been described to encode a CDC protein directly involved in cell cycle regulation (Millar and Russell 1992). Contrary, genes grouped in this same category and related to DNA processing were mostly negatively regulated by the MAPK pathway, for example genes encoding DNA helicases, DNA repair proteins and histone acetyltransferase (Table 1). Differential regulation by the PMM MAPK pathway of genes related to cell cycle supports studies performed on other eukaryotic organisms including fungi, which postulate that this pathway regulates positively or negatively the cell cycle of these organisms when sensing environmental or stress stimuli (Wilkinson and Millar 2000; Clotet and Posas 2007; Carbó and Pérez-Martín 2010).
Within the Transcription category, different genes encoding transcription factors were positively regulated by the PMM MAPK pathway (Table 2), including the following ones: um10426, encoding the PacC transcription factor; um15103, related to transcription factor AtfA; um03588, related to transcription factor Medusa; and um02052, the homologue of white collar 1 (WC1) gene from Neurospora crassa with 2.0-, 2.0-, 2.5- and 2.7-fold changes, respectively. Regarding PacC, it has been demonstrated that in most fungi, including U. maydis, pH sensing is carried out only by the PAL/RIM pathway, involving PacC (Ramon et al. 1999; Davis et al. 2000; Peñalva and Arst 2002; Lamb and Mitchell 2003; Aréchiga-Carvajal and Ruiz-Herrera 2005; Hua et al. 2010; Cervantes-Chávez et al. 2010; Franco-Frías et al. 2014). This observed increase in expression under the control of the MAPK pathway may be due to a cross talk between both signaling pathways (Fonseca-Garcia et al. 2012). The AtfA transcription factor has been described in A. nidulans and Schizosaccharomyces pombe as a bZIP-type transcription factor involved in response to different types of stress (Balázs et al. 2010). Medusa type transcription factors act in concert with other transcription factors and microRNAs forming a network that regulates the expression of a large number of genes, including some involved in the formation of tumors in animals (Guo et al. 2011). And WC1 gene in Neurospora is involved in the circadian feedback loops and light sensing (Ballario et al. 1996). The overexpression of transcription factors by the PMM MAPK pathway was expected since, as was mentioned above, this pathway activates different transcription factors that in turn regulate the expression of different genes or other transcription factors in response to the sensed stimuli. However, genes directly involved in RNA processing and grouped in this category were down-regulated, and among them, some involved in transcription, protein elongation, splicing factor, aminoacyl-tRNA hydrolases, etc. (Table 2). These latter data are in agreements with the negative effect of the PMM MAPK pathway on processes involved in DNA metabolism (see above).
Interestingly, most of the genes grouped in the Cellular Transport category that encode proteins involved in intracellular transport of different substances including metals such as, zinc, iron, calcium and sodium between organelles, or encode secretion proteins, such as effectors, were generally repressed by the PMM MAPK (Table 3). These data indicate that the cellular transport processes and protein secretion are mostly negatively regulated by the MAPK pathway during saprophytic growth of the fungus.
A category of genes with relevance considering the role of the MAPK pathway was the class of Signal Transduction Mechanism. Interestingly, genes associated within this category, as well as GTPases were grouped here, and most of them were positively regulated by the PMM MAPK pathway (Table 4). Among the genes overexpressed, we may cite the following: (1) um05656, related to Sok1 protein, a suppressor of kinases in S. cerevsiae (Ward and Garrett 1994); (2) um15092, encoding a probable Pbs2-tyrosine protein kinase of the MAP kinase kinase family that specifically phosphorylate threonine and tyrosine residues of the MAPK protein Hog1p, and is essential for the survival of the yeast under conditions of high osmolarity (Wurgler-Murphy et al. 1997); (3) um11007, that encodes a probable GTP-binding protein Rab5c, a member of the family of small GTPases that regulate membrane traffic, and whose role has been suggested in the mitotic cell cycle of eukaryotic cells (Singer-Krüger et al. 1994; Chiariello et al. 1999); and (4) um02382, encoding the Mfa1-a1-specific pheromone mating factor a1 (Bölker et al. 1992) with 3.3-, 3.6-, 5.1- and 13.2-fold change, respectively. Also, we identified genes encoding serine/threonine protein kinases and genes encoding MAPK proteins, for example: um03282, um10855, um04543, um15092 and Crk1 (um11410 with 2.9-fold change), described as a novel MAPK protein involved in the activation of the transcription factor Prf1, and therefore in mating and pathogenesis in U. maydis (Garrido et al. 2004). These results may be related to the recently described additional U. maydis MAPK pathways involved in the integrity of the cell wall, and perhaps the possible pathway responding to osmotic stress (Carbó and Pérez-Martín 2010; Perez-Nadales et al. 2014), and more important, the possible existence of a cross talk among them. In the category of Biogenesis of Cellular Component, different genes related to synthesis of the membrane and cell wall were found to be up-regulated by the PMM MAPK pathway (Table 5). Among these, we may cite genes related to cell wall biogenesis, e.g., those encoding chitin deacetylases, glucan synthases, chitinases and chitin synthases. One example is gene um01640 encoding GAS1, whose homologues have been described in Candida glabrata as of importance for cell wall biosynthesis (Weig et al. 2001). Also up-regulated was gene um05811 encoding KRE6, a protein involved in the synthesis of β 1,6-glucans. These results contrast with the observation that during U. maydis dimorphism induced by pH change, KRE6 was down-regulated (Martínez-Soto and Ruiz-Herrera 2013; Robledo-Briones and Ruiz-Herrera 2013). Finally, we may cite um04364 and um10211 encoding two Exg1-exo-β-1,3-beta-glucanases. Also some genes related to ribosome biogenesis were negatively regulated by the PMM MAPK pathway (see Table S1).
Finally, in the category Differentiation and Cell Fate, several genes related to dimorphism and cell morphology were found to be mostly up-regulated by the PMM MAPK pathway (Table 6). Among these genes, some related to actin may be important, taking into account their role in cell polarity and vesicle trafficking, for example: um11450 encoding Hgl1–Hgl1p is required for dimorphism and teliospore formation in U. maydis (Dürrenberger et al. 2001); um11246 is related to Bzz1, an actin assembly complex component involved in actin polymerization in S. cerevisiae (Soulard et al. 2002); um00896 encodes Kin7a-Kinesin-7a motor protein, probably involved in polar growth of hyphae; and um01671, related to Ysc84-protein, a member of a new class of actin-binding proteins described in yeasts as important for actin polarization and endocitosis (Robertson et al. 2009), which is involved in the organization of the actin cytoskeleton.
Regarding the possible nature of the transcription factors through which the PMM MAPK might control the expression of different genes, we found that the genes described as cyclin-dependent kinases, such as um10705, um02860 and um03234 (Table 1), posses the GGCCAT and TTGGT sequences in their promoter regions. These sequences are known to be binding sites for the Skn7 and Hap2 transcription factors, respectively. These data suggest that their regulation by the PMM MAPK pathway may occur through these two transcription factors, particularly Hap2 (um01597) that has already been described to be activated by the MAPK pathway (Brefort et al. 2009; Vollmeister et al. 2011), and has been described as a regulator of the master transcription factor, Prf1. Deletion of HAP2 affects mating and pathogenesis in U. maydis (Mendoza-Mendoza et al. 2009). The S. cerevisiae gene homologous to U. maydis Skn7 (um03346) have been described as involved in gene expression in response to changes in extracellular osmolarity, oxidative stress, thermal shock and cell wall integrity (Brown et al. 1993; Krems et al. 1996); we postulate that the PMM MAPK pathway through this transcription factor may regulate stress genes identified in this work (Fig. 1). Its homologues in C. albicans and Cryptococcus neoformans are important for virulence (Singh et al. 2004; Wormley et al. 2005); and its regulation in U. maydis has been described during A. thaliana infection (Martínez-Soto et al. 2013). In addition to binding sites for Hap2 and Skn7, we found the GATAA consensus sequence, which is recognized by the S. cerevisiae transcription factor Gln3 (see Tables 1, 4) involved in the expression of various genes in response to limiting nitrogen conditions (Minehart and Magasanik 1991; Xu et al. 1995). Accordingly we searched for potential homologues of this gene in U. maydis. The highest level of homology corresponded to gene um10417.
On the other hand and interestingly, 39 of the total of genes differentially regulated by the PMM MAPK pathway are grouped into twelve clusters; none of them was described as pathogenesis clusters by Kämper et al. (2006); and only one of them (Cluster V) has been described in U. maydis as involved in the biosynthesis of mannosylerythritol lipids (MELs) (Hewald et al. 2006) (Table 7). Moreover, for all genes grouped into clusters, we found in their promoter regions the presence of consensus sequences and binding sites for several transcription factors, and among the most represented are: AGGGG, binding sequence for MNS2, MSN4 and RGM1 transcription factors; AGACGC, binding sequence for ARG80 transcription factor; and also binding sequence for HAP2, GLN3, SKN7 and RIM101 or PacC (CGCCAAG) previously discussed. These data suggest that the regulation of gene clusters by the PMM MAPK pathway is through these transcription factors. All these transcription factors have been described in S. cerevisiae, e.g, MNS2 and MSN4 are transcriptional activators in stress response (Martínez-Pastor et al. 1996); RGM1 induces the expression of metabolism genes (Estruch 1991); and ARG80 is a transcriptional activator of arginine-responsive genes (Dubois et al. 1987). Also, possible homologous genes for MSN2, MSN4, RGM1 and ARG80 were identified in U. maydis (um00946, um12004, um02038 and um01224, respectively).
Agreeing with the fundamental role of the PMM MAPK pathway in the U. maydis dimorphic transition, we found that 60 of the 154 genes reported as differentially regulated during U. maydis dimorphism induced in vitro by a change in pH (Martínez-Soto and Ruiz-Herrera 2013) were regulated by the PMM MAPK pathway. Of these, 40 (67 %) were overexpressed and 20 (33 %) were repressed (see Table S2). In addition, 80 of the 345 genes reported as differential during U. maydis mycelial growth induced by the bE/bW heterodimer (Heimel et al. 2010) were found as differentially regulated by the PMM MAPK pathway; 60 of them (75 %) were up-regulated and 20 (25 %) down-regulated (see Table S3). Differential regulation of these genes suggests a mechanism for the regulation of fungal morphogenesis by this pathway.
In conclusion, these results of the first global analysis of the regulation of expression of the whole genome of a fungus, in this case U. maydis, demonstrate the importance of these studies for comprehension of the complex regulatory networks in these organisms. Accordingly, our data demonstrate that the PMM MAPK pathway positively regulates, directly or through specific transcription factors, genes involved in a large number of physiological processes in U. maydis, including the cell cycle, other signal transduction mechanisms, synthesis of membrane and cell wall, and dimorphism; and that negatively regulates DNA and RNA processing, and ribosome biogenesis, among other processes. Interestingly, these results demonstrate that the regulatory capacities of this pathway exceed what was previously known about its roles, showing the extreme complexity of its possible cross talks with other regulatory networks. No doubt that the data reported here will contribute to the understanding of the operation of the MAPK pathways in this and other fungi, and possibly even in higher organisms, considering that they are conserved in the eukaryotes.
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Acknowledgments
This work was supported by Consejo Nacional de Ciencia y Tecnología (CONACYT), Mexico. Thanks are given to Dr. Scott Gold for permission to use his microarray design, and QFB Claudia Geraldine León-Ramirez, IBQ Fernando Emilio Pérez-García, Biol. Mayela Fernanda Salazar-Chávez and M.S. Guillermo Antonio Silva-Martínez for assistance in some analyses. DMS is a doctoral student supported by a fellowship from CONACYT (México). JRH is Emeritus National Professor, México.
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Communicated by Erko Stackebrandt.
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Martínez-Soto, D., Ruiz-Herrera, J. Regulation of the expression of the whole genome of Ustilago maydis by a MAPK pathway. Arch Microbiol 197, 575–588 (2015). https://doi.org/10.1007/s00203-015-1087-2
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DOI: https://doi.org/10.1007/s00203-015-1087-2