Abstract
Needle-to-stem unit rate (NESTUR) is a stem growth index of conifer seedlings that measures the efficiency of stemwood production per unit of foliage growth. The random amplified polymorphic DNA (RAPD) technique was applied to haploid DNA from the megagametophytes of a full-sib radiata pine cross to find markers linked to factors controlling the NESTUR trait. Using the bulked segregant analysis approach, 23 of 933 primers displayed putative linkage to factors controlling NESTUR. Based on the genotypic analysis of 174 individuals, two quantitative trait loci (QTLs) controlling NESTUR were identified at ANOVA P-levels of 0.01–0.001. The QTLs were identified by RAPD markers OPE-06450 and OPA-101200, which were linked to each other (r=7%), and UBC-333550, which was not linked to the other two. Linkage to components of NESTUR (increments in stem diameter and stem volume) was demonstrated for UBC-333550, while the others were not linked to NESTUR components.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Additional information
Received: 18 December 1996/Accepted: 24 January 1997
Rights and permissions
About this article
Cite this article
Emebiri, L., Devey, M., Matheson, A. et al. Linkage of RAPD markers to NESTUR, a stem growth index in radiata pine seedlings. Theor Appl Genet 95, 119–124 (1997). https://doi.org/10.1007/s001220050539
Issue Date:
DOI: https://doi.org/10.1007/s001220050539