Abstract
A comparison betweeen the electropherograms of the spelt and wheat cultivars showed specific differences in the gliadin band patterns which provided the possibility of a clear classification into spelt or wheat. A special nomenclature was developed to be able to improve the presentation of the gliadin band pattern of spelt, which is different from that of wheat. This nomenclature, however, has not yet been applied to other cereals. The gliadin band patterns were presented in a schematic form. As a parameter for comparison, idealized band patterns of both wheat and spelt were developed by comparing the proportions of the bands of all available types. When comparing the gliadin band patterns of the spelt cross-breeds with their corresponding parental generations, it was noted that the same parental bands were not always transmitted and that the cross-breeds showed differences in the intensity, mobility, occurrence, and the splitting of single bands. In general it can be said that the band pattern of the daughter generation — even in the examined F5 and F6 generations — is more similar to the band pattern of the mother than to that of the father, which proves a maternal effect.
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References
Autran JC, Bourdet A (1976) Elektrophoretische Bestimmung der Gliadine zur Erkennung von Weizenmischungen. Getreide, Mehl und Brot 30:8–203
Baker RJ, Bushuk W (1978) Inheritance of differences in gliadin electropherograms in the progeny of ‘Neepawa’ and ‘Pitic 62’ wheats. Can J Plant Sci 58:325–329
Branlard G (1983) Study of genetic determination of 20 gliadin bands. Theor Appl Genet 64:155–162
Bushuk W, Zillman RR (1979) Wheat cultivar identification by gliadin electropherograms. II. Effects of environmental and experimental factors on the gliadin electropherogram. Can J Sci 59:281–286
Ciaffi M, Dominici L, Lafiandra D, Porceddu E (1992) Seed storage proteins of wild wheat progenitors and their relationships with technological properties. Hereditas 116:315–322
Dach Kevitch T, Redaelli R, Biancardi AM, Metakovsky EV, Pogna NE (1993) Genetics of gliadins coded by the group-1 chromosomes in the high quality bread wheat cultivar ‘Neepawa’. Theor Appl Genet 86:389–399
Galili G, Feldman M (1983) Genetic control of endosperm proteins in wheat. 2. Variation in high-molecular-weight glutenin and gliadin subunits of Triticum aestivum. Theor Appl Genet 66:77–86
Galili G, Feldman M (1985) Genetic control of endosperm proteins in wheat. 3. Allocation to chromosomes and differential expression of high-molecular-weight glutenin and gliadin genes in intervarietal substitution lines of common wheat. Theor Appl Genet 69:583–589
Günzel G (1976) Sortendifferenzierung bei Weizen mittels Polyacrylamidgel-Elektrophorese von Gliadin. J. Acker- und Pflanzenbau 143:37–83
Jackson EA, Holt LM, Payne PI (1983) Characterization of high-molecular-weight gliadin and low-molecular-weight glutenin sub-units of wheat endosperm by two-dimensional electrophoresis and the chromosomal localisation of their controlling genes. Theor Appl Genet 66:29–37
Jones RW, Taylor NW, Senti FR (1959) Electrophoresis and fractionation of wheat gluten. Arch Biochem Biophys 84:363–376
Joppa LR, Khan K, Williams ND (1983) Chromosomal location of genes for gliadin polypeptides in durum wheat, Triticum turgidum L. Theor Appl Genet 64:289–293
Kling Chl (1991) Prüfung alter deutscher Dinkelsorten als Basis für Züchtungsprogramme. Proc 2nd Hohenheimer Dinkelkol-loquium, University of Hohenheim, Germany. All Design Verlag, Stuttgart, pp 27–44
Mecham DK, Kasarda DD, Qualset CO (1978) Genetic aspects of wheat gliadin proteins. Biochem Genet 16:831–853
Mecham DK, Kasarda DD, Qualset CO (1985) Identification of Western U.S. wheat varieties by polyacrylamide gel electrophoresis of gliadin proteins. Hilgardia 53, 7:1–32
Metakovsky EV (1991) Gliadin allele identification in common wheat. II. Catalogue of gliadin alleles in common wheat. J Genet Breed 45:325–344
Metakovsky EV, Baboev SK (1992) Polymorphism and inheritance of gliadin polypeptides in T. monococcum L. Theor Appl Genet 84:971–978
Metakovsky EV, Novoselskaya AY, Kopus MM, Sobko TA, Sozinov AA (1984a) Blocks of gliadin components in winter wheat detected by one-dimensional polyacrylamide gel electrophoresis. Theor Appl Genet 67:559–568
Metakovsky EV, Novoselskaya AY, Sozinov AA (1984 b) Genetic analysis of gliadin components in winter wheat using two-dimensional polyacrylamide gel electrophoresis. Theor Appl Genet 69:31–37
Metakovsky EV, Akhmedov MG, Sozinov AA (1986) Genetic analysis of gliadin-encoding genes reveals gene clusters as well as single remote genes. Theor Appl Genet 73:278–285
Metakovsky EV, Iakobashvili ZA, Naskidashvili PP, Sozinov AA (1988) Polymorphism of gliadin and intraspecies heterogenity of wheat Triticum macha Dek. et Men. Cereal Res Commun 16:52–59
Metakovski EV, Vaccino P, Accerbi M, Redaelli R, Pogna NE (1993) Polymorphism and spontaneous mutations at the gliadin-coding loci. In: Gluten proteins 1993, Association of Cereal Research, Detmold, Germany, pp 218–226
Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Ann Rev Plant Physiol 38:141–153
Payne PI, Holt LM, Lawrence GJ, Law CN (1981/1982) The genetics of gliadin and glutenin, the major storage proteins of the wheat endosperm. Qualitas P1 Plant Food Hum Nutr 31:229–241
Payne PI, Jackson EA, Holt LM, Law CN (1984) Genetic linkage between endosperm storage protein genes on each of the short arms of chromosomes 1A and 1B in wheat. Theor Appl Genet 67:235–243
Redaelli R., Metakovsky EV, Davidov SD, Pogna NE (1994) Two-dimensional mapping of gliadins using biotypes and null mutants of common wheat cultivar ‘Saratovskaya 29’. Hereditas 121:131–137
Skerritt JH, Martinuzzi O, Metakovsky EV (1991) Chromosomal control of wheat gliadin protein epitopes: analysis with specific monoclonal antibodies. Theor Appl Genet 82:44–53
Sozinov AA, Poperelya FA (1980) Genetic classification of prolamines and its use for plant breeding. Ann. Technol Agric 29:229–245
Westermeier, R (1990) Elektrophorese-Praktikum, VHC Verlag, Germany
Woychick JH, Boundy JA, Dimler RJ (1961) Starch gel electrophoresis of wheat gluten proteins with concentrated urea. Arch Biochem Biophys 94:477–482
Wrigley CW, Sheperd KW (1974) Identification of Australian wheat cultivars by laboratory procedures. Examination of pure samples of grain. Aust J Exp Agric Anim Husb 14:796–804
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Communicated by H. F. Linskens
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Harsch, S., Günther, T., Rozynek, B. et al. Characterization of spelt (Triticum spelta L.) forms by gel electrophoretic analyses of seed storage proteins. I. The gliadins. Theoret. Appl. Genetics 94, 52–60 (1997). https://doi.org/10.1007/s001220050381
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DOI: https://doi.org/10.1007/s001220050381