Summary
More than 80 different gliadin electrophoretic patterns (spectra) have been found in 109 accessions of the diploid wheat Triticum monococcum. Each pattern consists of 15–20 gliadin bands. Some patterns are clearly related and might arise from one another through single mutations in the gliadin-coding loci. From the analysis of 15 grains of each, only 61 accessions were found to be uniform; others consisted of two or more grain variants differing in their gliadin spectrum. An analysis of F2 grains from three crosses between different accessions showed that groups (blocks) of components are jointly and codominantly inherited. Two independent major Gli loci were established. The close resemblance of the composition of some blocks of T. monococcum to some of those in polyploid wheats indicates that one locus in each T. monococcum genotype is located on chromosome 1A (Gli-A1) and the other on 6A (Gli-A2). However, the blocks of T. monococcum include more bands than corresponding (equivalent) blocks of polyploid wheats. Two out of 275 F2 grains of the cross k-14244 x k-20409 were found to have gliadin spectra which can be explained as a result of intralocus recombination. Also, a second gliadin-coding locus on chromosome 1A was found in the cross k-46140 x k-46753. This locus recombines with the main Gli-A1 locus with a frequency of about 22% and was clearly analogous to the additional Gli locus found earlier on chromosome 1A of certain polyploid wheats.
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References
Appleyard DB, McCausland J, Wrigley CW (1979) Checking the identity and origin of off-types in the propagation of pedigree wheat seed. Seed Sci Technol 7:459–466
Baboev SK, Filatenko AA, Iakobashvili ZA, Metakovsky EV (1990) Polymorphism of gliadin in diploid wheat species with an A genome (in Russian). Genetika 26:2166–2176
Chapman V, Miller TE, Riley R (1976) Equivalence of the A genome of bread wheet and that of Triticum urartu. Genet Res 27:69–76
Dvorak J (1979) The relationship between the genome of Triticum urartu and the A and B genomes of Triticum aestivum. Canad J Genet Cytol 18:371–377
Fedak G (1985) Alien species as sources of physiological traits for wheat improvement. Euphytica 34:673–680
Galili G, Felsenburg T, Levy AA, Altschuler Y, Feldman M (1988) Inactivity of high-molecular-weight glutenin genes in diploid and tetraploid wheats. Proc 7th Int Wheat Genet Symp (Cambridge) 1:81–86
Galili S, Galili G, Feldman M (1991) Chromosomal location of genes for Rubisco small subunit and Rubisco-binding protein in common wheat. Theor Appl Genet 81:98–104
Kasarda DD (1980) Structure and properties of α-gliadins. Ann Technol Agric 29:151–173
Kerby K, Kuspira J (1987) The phylogeny of the polyploid wheats Triticum aestivum (bread wheat) and Triticum turgidum (macaroni wheat). Genome 29:722–737
Kihara H (1954) Origin of wheat. Wheat Inf Serv 1:36–41
Kihara H (1982) Wheat studies —retrospect and prospects. Elsevier Scientific, Amsterdam
Konarev VG, Gavrilyuk IP, Gubareva NK, Peneva TI (1979) Seed proteins in genome analysis, cultivar identification and documentation of cereal genetic resources: a review. Cereal Chem 56:272–278
Kudryavtsev AM, Metakovsky EV, Sozinov AA (1988) Polymorphism and inheritance of gliadin components controlled by chromosome 6 A of spring durum wheat. Biochem Genet 26:693–703
Kuspira J, Maclagan J, Bhambhani RN, Sadasivaiah RS, Kim NS (1989) Genetic and cytogenetic analyses of the A genome of T. monococcum L. V. Inheritance and linkage relationships of genes determining the expression of 12 qualitative characters. Genome 32:869–881
Lafiandra D, Colaprico G, Kasarda DD, Porceddu E (1987) Null alleles for gliadin blocks in bread and durum wheat cultivars. Theor Appl Genet 74:610–616
Lagudah ES, Halloran GM (1988a) Phylogenetic relationships of Triticum tauschii the D genome donor to hexaploid wheat 1. Variation in HMW subunits of glutenin and gliadins. Theor Appl Genet 75:592–598
Lagudah ES, Halloran GM (1988b) Phylogenetic relationships of Triticum tauschii the D genome donor to hexaploid wheat 2. Inheritance and chromosomal mapping of the HMW subunits of the glutenin and gliadin gene loci of T. tauschii. Theor Appl Genet 75:599–605
Levi AA and Feldman M (1987) Increase in grain protein percentage in high-yielding common wheat breeding lines by genes from wild tetraploid wheat. Euphytica 36:353–359
Metakovsky EV (1990) The value of gliadin biotypes in commercial cultivars of wheat. In: Proc 4th Int Workshop on Gluten Proteins (Winnipeg) Bushuk W and Tkachuk R (eds) AACC, pp 569–580
Metakovsky EV (1991) Gliadin allele identification in common wheat 2. Catalogue of gliadin alleles in common wheat. J Genet Breed 45:325–344
Metakovsky EV and Baboev SM (1992) Polymorphism of gliadin and unusual gliadin alleles in Triticum boeoticum. Genome (in press)
Metakovsky EV, Iakobashvili ZA (1990) Homology of chromosomes of Triticum masha Dek. et Men. and Triticum aestivum L. as shown with the help of genetic markers. Genome 33:755–757
Metakovsky EV, Novoselskaya AYu (1991) Gliadin allele identification in common wheat 1. Methodological aspects of the analysis of gliadin pattern by one-dimensional polyacrylamide-gel electrophoresis. J Genet Breed 45:317–324
Metakovsky EV, Sozinov AA (1987) Organization, variability and stability of the family of gliadin-coding genes In: Lasztity R, Bekes F (eds) Wheat: genetic data. Proc 3rd Int Workshop on Gluten Proteins (Budapest, 1987) World Sci, pp 30–45
Metakovsky EV, Novoselskaya AYu, Kopus MM, Sobko TA, Sozinov AA (1984) Blocks of gliadin components in winter wheat detected by one-dimensional polyacrylamide gel electrophoresis. Theor Appl Genet 67:559–568
Metakovsky EV, Il'ina LG, Galkin AN, Koval SF, Sozinov AA (1987) Allelic variants of blocks of gliadin components in Saratov wheats (in Russian). Selektsia i Semenovod. 1:11–15
Metakovsky EV, Kudryavtsev AM, Iakobashvili ZA, Novoselskaya AYu (1989) Analysis of phylogenetic relations of durum, carthlicum and common wheats by means of comparison of alleles of gliadin-coding loci. Theor Appl Genet 77:881–887
Metakovsky EV, Chernakov VM, Shamanin VP (1990) Gliadin polymorphism in spring common wheat cultivars of the Omsk region (in Russian). Dokladi VASkHNIL 9:10–14
Metakovsky EV, Knezevic D, Javornik B (1991) Gliadin allele composition of Yugoslav winter wheat cultivars. Euphitica 54:285–295
Nevo E, Beiles A (1989) Genetic diversity of wild emmer wheat in Israel and Turkey. Theor Appl Genet 77:421–455
Payne PI, Jackson EA, Holt LM, Law CN (1984) Genetic linkage between endosperm storage protein genes on each of the short arms of chromosomes 1A and 1b in wheat. Theor Appl Genet 67:235–243
Pogna NE, Dal Belin Peruffo A, Boggini G, Corbellini M (1982) Analysis of wheat varieties by gliadin electrophoregrams II. Nature, origin and quality of biotypes present in six Italian common wheat varieties. Genet Agrar 36:143–154
Pomortsev AA, Netsvetaev VP, Sozinov AA (1985) Polymorphism of hordein in barley Hordeum vulgare (in Russian). Genetika 21:629–636
Porceddu E, Ceoloni C, Lafiandra D, Tanzarella OA, Scarascia Mugnozza GT (1988) Genetic resources and plant breeding: problems and prospects. Proc 7th Int Wheat Genet Symp (Cambridge) 1:7–22
Riley R (1965) Cytogenetics and the evolution of wheat. In: Hutchinson J (ed) Essays on crop plant evolution. University Press, Cambridge, pp 103–122
Sharma HC, Gill BS (1983) Current status of wide hybridization in wheat. Euphytica 32:17–31
Smith-Huerta NL, Huerta AJ, Barnhart D, Waines JG (1989) Genetic diversity in the wild diploid wheats Triticum monococcum var. boeoticum and T. urartu (Poaceae). Theor Appl Genet 78:260–264
Sobko TA (1984) Identification of a new locus which controls the synthesis of alcohol-soluble protein in the endosperm of winter common wheat (in Ukrainian). J Agric Sci (Kiev) 7:78–80
Sozinov AA, Poperelya FA (1979) Genetic classification of prolamines and its use for plant breeding. Ann Technol Agric 29:229–245
The TT (1973) Chromosome location of genes conditioning stem rust resistance transferred from diploid to hexaploid wheat. Nature 241:256
Waines JG, Payne PI (1987) Electrophoretic analysis of the high-molecular-weight glutenin subunits of Triticum monococcum, T. urartu, and the A genome of bread wheat (T. aestivum). Theor Appl Genet 74:71–76
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Communicated by Yu. Gleba
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Metakovsky, E.V., Baboev, S.K. Polymorphism and inheritance of gliadin polypeptides in T. monococcum L.. Theoret. Appl. Genetics 84, 971–978 (1992). https://doi.org/10.1007/BF00227412
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DOI: https://doi.org/10.1007/BF00227412