Abstract:
Objective and Design: The purpose of this study was to compare chemotactic activity of IL-8 alone with that of IL-8 reacted with rabbit α-macroglobulins (αM) in vivo.¶Methods: Initially the binding of recombinant human IL-8 (rhIL-8) to rabbit αM was studied. 125I-labeled rhIL-8 was incubated with αM, and electrophoresed on native 5% gels or SDS-polyacrylamide 4-20% gradient gels. Next, rhIL-8 or rhIL-8 bound to αM was administered via an endotracheal tube to rabbit's lungs.¶Treatment: An endotracheal tube was wedged into a segment of the lobe of each lung, and a sample instilled through the tube into this segment. After 4 h the lungs were lavaged.¶Results: rhIL-8 bound to αM retained its full chemotactic activity in vitro but exhibited a diminished ability to induce the influx of neutrophils into the rabbit lung.¶Conclusions: The data suggest that αM may facilitate IL-8 clearance from the lung.
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Received 3 January 2000; returned for revision 8 February 2000; accepted by G. W. Carter 28 April 2000
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Kurdowska, A., Fujisawa, N., Peterson, B. et al. Specific binding of IL-8 to rabbit α-macroglobulin modulates IL-8 function in the lung. Inflamm. res. 49, 591–599 (2000). https://doi.org/10.1007/s000110050636
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DOI: https://doi.org/10.1007/s000110050636