Abstract
The authors’ own and literature data are summarized on interaction of 17 irreversible organophosphorus inhibitors with different types of cholinesterases (ChE): erythrocyte acetylcholinesterase (AChE), serum butyrylcholinesterase (BuChE), and cholinesterase of the Pacific squidTodarodes pacificus, in the presence of 9 substrates. The kinetic analysis of the “substrate protective effect” based on A.P. Brestkin’s equation is performed, and the current interpretation of individual components of this process is done. An essential effect of the inhibitor structure on individual phases of the reaction is revealed. Among choline substrates, only formylcholine did not show a protective effect. The inability of an uncationic substrate, phenylacetate, to regulate ChE reactivity is confirmed. Among the studied ChE, the highest substrate protective effect was revealed in the Pacific squid ChE.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Sadykov, A.S., Rozengart, E.V., Abduvakhabov, A.A., and Aslanov, Kh.A.,Kholinesterazy. Aktivnyi tsentr i mekhanizm deistviya (Cholinesterases. Active Center and Action Mechanism), Tashkent, 1976.
Brestkin, A.P., Kuznetsova, L.P., Moralev, S.N., and Rozengart, E.V.,Kholinesterazy nazemnykh zhivotnykh i gidrobiontov (Cholinesterases of Terrestrial Animals and Hydrobionts), Vladivostok, 1997.
Rozengart, E.V., Basova, N.E., Khovanskikh, A.E., and Epshtein, L.M., Various Aspects of Substrate Specificity of Cholinesterase from Optical Ganglia of the Pacific SquidTodarodes pacificus, Zh. Evol. Biokhim. Fiziol., 1996, vol. 32, pp. 384–392.
Yakovlev, V.A. and Volkova, R.I., Study of Cholinesterase Active Centers using Organophosphorus Inhibitors,Dokl. Akad. Nauk SSSR, 1962, vol. 146, pp. 217–220.
Main, A.R. and Dauterman, W.C., Determination of Bimolecular Rate Constant for the Reaction between Organophosphorus Inhibitors and Esterases in the Presence of Substrate,Nature, 1963, vol. 198, pp. 551–553.
Brestkin, A.P., Volkova, R.I., and Rozengart, E.V., On the Protective Effect of Acetylcholine at Interaction of Serum Cholinesterase with Organophosphorus Inhibitors,Dokl. Akad. Nauk SSSR, 1964, vol. 157, pp. 1459–1462.
Volkova, R.I., the Mechanism of Interaction of Cholinesterase with Irreversible Organophosphorus Inhibitors in the Presence of Substrate,Biokhimiya, 1965, vol. 30, pp. 292–301.
Rozengart, E.V., Interaction of O-ethyl-S-alkylmethylthiophosphonates with Cholinesterase in the Presence of Acetylcholine,Biokhimiya, 1965, vol. 30, pp. 344–349.
Volkova, R.I., Kinetics of Interaction of Cholinesterase with Irreversible Organophosphorus Inhibitors in the Presence of Phenylacetate,Biokhimiya, 1968, vol. 33, pp. 369–374.
Bogolyubova, G.M., Karpinskaya, E.V., Kulikova, A.I.,et al., Interaction of Cholinesterase with Organophosphorus Inhibitors in the Presence of Substrates of Various Structure,Biokhimiya, 1970, vol. 35, pp. 3153–1159.
Bogolyubova, G.M., Karpinskaya, E.V., Kulikova, A.I., and Rozengart, V.I., Protective Effect of Substrates at Inhibition of Various Cholinesterases by Organophosphorus Inhibitor,Biokhimiya, 1971, vol. 36, pp. 1075–1081.
Zhukovskii, Yu.G., On the Kinetics of Cholinesterase Hydrolysis of Substrate at Irreversible Inhibition of the Enzyme,Izvestiya Akad. Nauk SSSR, Ser. Biol., 1984, no. 1, pp. 55–61.
Yakovlev, V.A. and Volkova, R.I., Kinetics of Cholinesterase Interaction with Irreversible Inhibitors,Dokl. Akad. Nauk SSSR, 1959, vol. 128, pp. 843–846.
Wilson, I.B. and Cabib, E., Acetylcholinesterase: Enthalpies and Entropies Activations,J. Amer. Chem. Soc, 1956, vol. 78, pp. 202–207.
Yakovlev, V.A.,Kinetika fermentativnogo kataliza (Kinetics of Enzymatic Catalysis), Moscow, 1965.
Volkova, R.I., Kinetics of Enzymatic Hydrolysis of Phenylacetate,Biokhimiya, 1967, vol. 32, pp. 1253–1259,
Albert, A. and Sergeant, E.,Konstanty ionizatsii kislot i osnovanii (Constants of Ionization of Acids and Bases), Moscow, 1964.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Basova, N.E., Rozengart, E.V. & Khovanskikh, A.E. Kinetic analysis of the “substrate protective effect” in cholinesterases of different origin. J Evol Biochem Phys 36, 130–137 (2000). https://doi.org/10.1007/BF02754325
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02754325