Summary
A new fibroblast-like cell line, CyN, has been developed from muscle tissue of the marine fishCynoscion nebulosus (the spotted weakfish). A subline, CyN-1, was characterized fully and carried through 100 subcultures. CyN-1 is heteroploid and has a modal chromosome number of 49. The line is susceptible to LT-1, lymphocystis, eastern equine encephalitis and vesicular stomatitis viruses. Optimal growth occurred at 25° to 34° C in growth medium adjusted to 0.150m NaCl.
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This study was supported in part by the National Oceanographic and Atmospheric Administration, Office of Sea Grant No. 04-3-158-58.
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Middlebrooks, B.L., Ellender, R.D. & Wharton, J.H. Fish cell culture: A new cell line fromCynoscion nebulosus . In Vitro 15, 109–111 (1979). https://doi.org/10.1007/BF02618105
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DOI: https://doi.org/10.1007/BF02618105