Abstract
The immobilization of DNA on different solid supports has become an important issue in different fields ranging from medicine to analytical chemistry and, more recently, molecular electronics. Among the different immobilization procedures, adsorption is the simplest and the easiest to automate, avoiding the use of procedures based on previous activation/modification of the substrate and subsequent immobilization, which are tedious, expensive and time-consuming. Carbon-based materials are widely used for this task due to their electrochemical, physical and mechanical properties, their commercial availability, and their compatibility with modern microchip fabrication technology. Moreover, carbonaceous materials are widely used as transducers for electrochemical sensors. The knowledge of the adsorbed DNA morphology on carbon surfaces can be used to develop stable and functional DNA layers for their use in DNA analytical devices with improved properties. Presented here is a concise description of surface immobilization of DNA, oligonucleotides, and DNA derivatives by adsorption onto carbonaceous materials, and the properties of the DNA layer adsorbed on carbonaceous solid phase.
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Abbreviations
- A:
-
Adenine
- ABS:
-
Acetate buffer solution
- AFM:
-
Atomic force microscopy
- BDD:
-
Boron-doped diamond
- BLM:
-
Bilayer lipid membrane
- C:
-
Cytosine
- CNT:
-
Carbon nanotube
- CNTP:
-
Carbon nanotube paste
- CP:
-
Carbon paste
- dsDNA:
-
Double-stranded DNA or native DNA
- G:
-
Guanine
- GC:
-
Glassy carbon
- GC(ox) :
-
Anodized glassy carbon
- GEC:
-
Graphite epoxy composite
- HOPG:
-
Highly ordered pyrolytic graphite
- MWCNT:
-
Multi-wall carbon nanotube
- ODN:
-
Oligodeoxynucleotide
- PBS:
-
Phosphate buffer solution
- PG:
-
Pyrolytic graphite
- SCE:
-
Saturated calomel electrode
- ssDNA:
-
Single-stranded DNA or denatured DNA
- SWCNT:
-
Single-wall carbon nanotube
- T:
-
Thymine
References
Baltimore D (2001) Nature 409:814
Bowtell DDL (1999) Nature Genet [suppl] 21:25
Collins FS (1999) Nature Genet [suppl] 21:2
Lander ES (1999) Nature Genet [suppl] 21:3
Sanders GHW, Manz A (2000) Trends Anal Chem 19:364
Pividori MI, Merkoçi A, Alegret S (2000) Biosensor Bioelectron 15:291
Southern E, Mir K, Shchepinov M (1999) Nature Genet [suppl] 21:5
Paleček E (1958) Naturwiss 45:186
Paleček E (1960) Nature 188:656
Streat M, Malik DJ, Saha B (2004) Adsorption and ion-exchange properties of engineered activated carbons and carbonaceous materials. In: SenGupta AK, Marcus Y, Marinsky JA (eds) Ion exchange and solvent extraction, chap 1. Dekker, New York
Chu X, Kinoshita K (1997) Mater Sci Eng B 49:53
Regisser F, Lavoie MA, Champagne GY, Belanger D (1996) J Electroanal Chem 415:47
McCreery RL, Cline KK (1996) Carbon electrodes. In: Kissinger PT, Heineman WR (eds) Laboratory techniques in electroanalytical chemistry, chap 10. Dekker, New York
Hu IF, Karweik DH, Kuwana T (1985) J Electroanal Chem 188:59
Kepley LJ, Bard AJ (1988) Anal Chem 60:1459
Oliveira Brett AM, Chiorcea AM (2003) Langmuir 19:3830
Wu L, Zhou J, Luo J, Lin Z (2000) Electrochim Acta 45:2923
Alegret S (1996) Analyst 121:1751
Céspedes F, Martínez-Fàbregas E, Alegret S (1996) Trends Anal Chem 15:296
Adams RN (1958) Anal Chem 30:1576
Iijima S (1991) Nature 354:56
Sherigara BS, Kutner W, D'Souza F (2003) Electroanalysis 15:753
Tsui R, Siragusa L, Goronkin H (2000) C R Acad Sci Paris 1:875
Guo ZJ, Sadler PJ, Tsang SC (1998) Adv Mater 10:701
Xu J (2003) Proc IEEE 91:1819
Cai H, Cao XN, Jiang Y, He PG, Fang YZ (2003) Anal Bioanal Chem 375:287
Walther JH, Jaffe RL, Kotsalis EM, Werder T, Halicioglu T, Koumoutsakos P (2004) Carbon 42:1185
Li J, Ng HT, Cassell A, Fan W, Chen H, Ye Q, Koehne J, Han J, Meyyappan M (2003) Nano Lett 3:597
Oliveira Brett AM, Piedade JAP, Serrano SHP (2000) Electroanalysis 12:969
Wang J (1998) Biosensor Bioelectron 13:757
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York
Meinkoth J, Wahl G (1984) Anal Biochem 138:267
Reed KC, Mann DA (1985) Nucleic Acids Res 13:7207
Rasmussen SR, Larsen MR, Rasmussen SE (1991) Anal Biochem 198:138
Pang DW, Zhang M, Wang ZL, Qi YP, Cheng JK, Liu ZY (1996) J Electroanal Chem 403:183
Oliveira Brett AM, Vivan M, Fernandes IR, Piedade JAP (2002) Talanta 56:959
Oliveira Brett AM, Serrano SHP, Gutz I, La-Scalea MA, Cruz ML (1997) Electroanalysis 9:1132
Brett CMA, Oliveira Brett AM, Serrano SHP (1999) Electrochim Acta 44:4233
Oliveira Brett AM, Macedo TRA, Raimundo D, Marques MH, Serrano SHP (1998) Biosensor Bioelectron 13:861
Abreu FC, Goulart MOF, Oliveira Brett AM (2002) Biosensor Bioelectron 17:913
Lu X, Zhu K, Zhang M, Liu H, Kang J (2002) J Biochem Biophys Methods 52:189
Wang ZX, Liu DJ, Dong SJ (2001) Bioelectrochemistry 53:175
Wang ZX, Liu DJ, Dong SJ (2001) Biophys Chem 89:87
Pedano ML, Rivas GA (2003) Biosensor Bioelectron 18:269
Tang TC, Huang HJ (1999) Electroanalysis 11:1185
Wang HS, Ju HX, Chen HY (2001) Electroanalysis 13:1105
Wang HS, Ju HX, Chen HY (2002) Anal Chim Acta 461:243
Wang HS, Ju HX, Chen HY (2002) Electroanalysis 14:1615
Oliveira Brett AM, da Silva LA, Brett CMA (2002) Langmuir 18:2326
Zen JM, Chang MR, Ilangovan G (1999) Analyst 124:679
Napier ME, Thorp HH (1999) J Fluorescence 9:181
Xu C, Cai H, He P, Fang Y (2001) Analyst 126:62
Cai H, Wang Y, He P, Fang Y (2003) Anal Chim Acta 469:165
Zhu N, Cai H, He P, Fang Y (2003) Anal Chim Acta 481:181
Luo L, Liu J, Wang Z, Yang X, Dong S, Wang E (2001) Biophys Chem 94:11
Li Z, Wang H, Dong S, Wang E (1997) Anal Sci 13:305
Wang J, Jiang M, Mukherjee B (1999) Anal Chem 71:4095
Cai H, Zhu N, Jiang Y, He P, Fang Y (2003) Biosensor Bioelectron 18:1311
Wang J, Jiang M, Fortes A, Mukherjee B (1999) Anal Chim Acta 402:7
Nikolelis DP, Hianik T, Krull UJ (1999) Electroanalysis 11:7
Siontorou CG, Oliveira Brett AM, Nikolelis DP (1996) Talanta 43:1137
Tong Y, Han X, Song Y, Jiang J, Wang E (2003) Biophys Chem 105:1
Siontorou CG, Nikolelis DP, Tarus B, Dumbrava J, Krull UJ (1998) Electroanalysis 10:691
Brabec V, Dryhurst G (1978) J Electroanal Chem 89:161
Oliveira Brett AM, Chiorcea AM (2003) Electrochem Comm 5:178
Chiorcea AM, Oliveira Brett AM (2002) Bioelectrochemistry 55:63
Hashimoto K, Ito K, Ishimori Y (1994) Anal Chim Acta 286:219
Rusling JF, Zhou L, Munge B, Yang J, Estavillo C, Schenkman JB (2000) Faraday Discuss 116:77
Palecek E, Fojta M, Jelen F (2002) Bioelectrochemistry 56:85
Fojta M, Havran L, Kizek R, Billová S (2002) Talanta 56:867
Ivandini TA, Sarada BV, Rao TN, Fujishima A (2003) Analyst 128:924
Rao TN, Yagi I, Miwa T, Tryk DA, Fujishima A (1999) Anal Chem 71:2506
Wang J, Cai X, Wang J, Jonsson C, Palecek E (1995) Anal Chem 67:4065
Wang J, Cai X, Rivas G, Shiraishi H (1996) Anal Chim Acta 326:141
Wang J, Zhang XJ, Parrado C, Rivas G (1999) Electrochem Comm 1:197
Wang J, Cai X, Jonsson C, Balakrishnan M (1996) Electroanalysis 8:20
Wang J, Rivas G, Cai X, Dontha N, Shiraishi H, Luo D, Valera FS (1997) Anal Chim Acta 337:41
Wang J, Rivas G, Fernández JR, Lopez Paz JL, Jiang M, Waymire R (1998) Anal Chim Acta 375:197
Wang J, Kawde AN, Sahlin E, Parrado C, Rivas G (2000) Electroanalysis 12:917
Wang J, Chicharro M, Rivas G, Cai X, Dontha N, Farias PAM, Shiraishi H (1996) Anal Chem 68:2251
Palecek E, Fojta M, Tomschik M, Wang, J (1998) Biosensor Bioelectron 13:621
Cai X, Rivas G, Farias PAM, Shiraishi H, Wang J, Palecek E (1996) Anal Chim Acta 332:49
Wang J, Bollo S, Lopez Paz JL, Sahlin E, Mukherjee B (1999) Anal Chem 71:1910
Wang J, Gründler P, Flechsing GU, Jasinski M, Rivas G, Sahlin E, Lopez Paz JL (2000) Anal Chem 72:3752
Korbut O, Bucková M, Tarapcík P, Labuda J, Gründler P (2001) J Electroanal Chem 506:143
Korbut O, Bucková M, Labuda J, Gründler P (2003) Sensors 3:1
Erdem A, Kerman K, Meric B, Akarca US, Ozsoz M (1999) Electroanalysis 11:586
Erdem A, Meric B, Kerman K, Dalbasti T, Ozsoz M (1999) Electroanalysis 11:1372
Meric B, Kerman K, Ozkan D, Kara P, Erensoy S, Akarca US, Mascini M, Ozsoz M (2002) Talanta 56:837
Radi A (1999) Anal Chim Acta 386:63
Hu C, Hu S (2004) Electrochim Acta 49:405
Kara P, Kerman K, Ozkan D, Meric B, Erdem A, Nielsen PE, Ozsoz M (2002) Electroanalysis 14:1685
Dantoni P, Serrano SHP, Oliveira Brett AM, Gutz IGR (1998) Anal Chim Acta 366:137
Rosatto SS, De Oliveira Neto G, Kubota LT (2001) Electroanalysis 13:445
Lee TY, Kim HJ, Moon JO, Shim YB (2004) Electroanalysis 16:821
Gil ES, Kubota LT (2000) Bioelectrochemistry 51:145
Pividori M I, Merkoçi A, Alegret S (2003) Biosensor Bioelectron 19:473
Pividori M I, Merkoçi A, Barbé J, Alegret S (2003) Electroanalysis 15:1815
Pividori M I, Alegret S (2003) Anal Lett 36:1669
Erdem A, Pividori M I, del Valle M, Alegret S (2004) J Electroanal Chem 567:29
Pividori M I, Merkoçi A, Alegret S (2001) Biosensor Bioelectron 16:1133
Pividori M I, Merkoçi A, Alegret S (2001) Analyst 126:1551
Teh HF, Yang X, Gong H, Tan SN (2004) Electroanalysis 16:769
Li J, Chia LS, Goh NK, Tan SN (1999) J Electroanal Chem 460:234
Wang J, Cai X, Rivas G, Shiraishi H, Dontha N (1997) Biosensor Bioelectron 12:587
Wang J, Rivas G, Cai X (1997) Electroanalysis 9:395
Marrazza G, Chianella I, Mascini M (1999) Biosensor Bioelectron 14:43
Chiti G, Marrazza G, Mascini M (2001) Anal Chim Acta 427:155
Marrazza G, Chianella I, Mascini M (1999) Anal Chim Acta 387:297
Azek F, Grossiord C, Joannes M, Limoges B, Brossier P (2000) Anal Biochem 284:107
Bucková M, Labuda J, Sandula J, Krizková L, Stepánek I, Duracková Z (2002) Talanta 56:939
Labuda J, Bucková M, Heilerová L, Caniová-Ziaková A, Brandsteterová E, Mattusch J, Wennrich R (2002) Sensors 2:1
Labuda J, Bucková M, Jantová S, Stepánek I, Surugiu I, Danielsson B, Mascini M (2000) Fresenius J Anal Chem 367:364
Wang J, Kawde AN, Sahlin E (2000) Analyst 125:5
Wang J, Kawde AN, Erdem A, Salazar M (2001) Analyst 126:2020
Wang J, Kawde AN (2001) Anal Chim Acta 431:219
Matyshevska OP, Karlash AY, Shtogun YV, Benilov A, Kirgizov Y, Gorchinskyy KO, Buzaneva EV, Prylutskyy YI, Scharff P (2001) Mater Sci Eng C 15:249
Buzaneva E, Karlash A, Yakovkin K, Shtogun Y, Putselyk S, Zherebetskiy D, Gorchinskiy A, Popova G, Prilutska S, Matyshevska O, Prilutskyy Y, Lytvyn P, Scharff P, Eklund P (2002) Mater Sci Eng C 19:41
Dovbeshko GI, Repnytska OP, Obraztsova ED, Shtogun YV (2003) Chem Phys Lett 372:432
O'Connell MJ, Boul P, Ericson LM, Huffman C, Wang Y, Haroz E, Kuper C, Tour J, Ausman KD, Smalley RE (2001) Chem Phys Lett 342:265
Wang G, Xu JJ, Chen HY (2002) Electrochem Commun 4:506
Guo M, Chen J, Nie L, Yao S (2004) Electrochim Acta 49:2637
Wang J, Kawde AN, Musameh M (2003) Analyst 128:912
Wang J, Li M, Shi Z, Li N, Gu Z (2004) Electroanalysis 161:140
Xu Y, Jiang Y, Cai H, He PG, Fang YZ (2004) Anal Chim Acta 516:19
Pedano ML, Rivas GS (2004) Electrochem Commun 6:10
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Pividori, M.I., Alegret, S. DNA Adsorption on Carbonaceous Materials. In: Wittmann, C. (eds) Immobilisation of DNA on Chips I. Topics in Current Chemistry, vol 260. Springer, Berlin, Heidelberg. https://doi.org/10.1007/b136064
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DOI: https://doi.org/10.1007/b136064
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