Abstract
Of the four thermotolerant, osmotolerant, flocculating yeasts (VS1, VS2, VS3 and VS4) isolated from the soil samples collected within the hot regions of Kothagudem Thermal Power Plant, located in Khammam Dt., Andhra Pradesh, India, VS1 and VS3 were observed as better performers. They were identified as Saccharomyces cerevisiae. VS1 and VS3 were tested for their growth characteristics and fermentation abilities on various carbon sources including molasses at 30 °C and 40 °C respectively. More biomass and fermentation was observed in sucrose, fructose and glucose. Maximum amount of ethanol produced by VS3 containing 150 (g/l) of these substrates were 74, 73, and 72 (g/l) at 30 °C and 64, 61 and 63 (g/l) at 40 °C respectively. With molasses containing 14% sugar, the amount of ethanol produced by VS3 was 53.2 and 45 (g/l) at 30 °C and 40 °C respectively. VS3 strain showed 12% W/V ethanol tolerance. VS3 strain was also characterised for its ethanol producing ability using various starchy substrates in solid state and submerged fermentation. More ethanol was produced in submerged than solid state fermentation.
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Received: 22 March 1999
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Kiransree, N., Sridhar, M. & Venkateswar Rao, L. Characterisation of thermotolerant, ethanol tolerant fermentative Saccharomyces cerevisiae for ethanol production. Bioprocess Engineering 22, 243–246 (2000). https://doi.org/10.1007/PL00009114
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DOI: https://doi.org/10.1007/PL00009114