Abstract
Nickel ferrite (NiFe2O4) nanoparticles have been dispersed in chitosan solution in order to fabricate nanocomposite films. Horseradish peroxidase (HRP) has been immobilized onto this chitosan-NiFe2O4 nanocomposite film via physical adsorption. The size of the NiFe2O4 nanoparticles has been estimated using X-ray diffraction pattern and scanning electron microscopy (SEM) to be 40±9 nm. The chitosan-NiFe2O4 nanocomposite film and HRP/chitosan-NiFe2O4 bioelectrode have been characterized using SEM technique. The HRP/chitosan-NiFe2O4 nanocomposite bioelectrode has a response time of 4 s, linearity as 0.3 to 12mM of H2O2, sensitivity as 22 nA/mM. The effects of pH and the temperature of the immobilized HRP electrode have also been studied.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
E. C. Hurdis and J. H. Romeyn, Anal. Chem. 26, 320 (1954). http://dx.doi.org/10.1021/ac60086a016
S. Hanaoka, J. M. Lin and M. Yamada, Anal. Chim. Acta. 426, 57 (2001). http://dx.doi.org/10.1016/S0003-2670(00)01181-8
L. S. Zhang and G. T. F. Wong, Talanta 48, 1031 (1999). http://dx.doi.org/10.1016/S0039-9140(98)00312-9
C. Matsubara, N. Kawamoto and K. Takamura, Analyst 117, 1781 (1992). http://dx.doi.org/10.1039/an9921701781
Y. Xiao, H. X. Ju and H. Y. Chen, Anal. Biochem. 278, 22 (2000). http://dx.doi.org/10.1006/abio.1999.4360
L. Qian and X. Yang, Talanta 68, 721 (2006). http://dx.doi.org/10.1016/j.talanta.2005.05.030
P. He and N. Hu, Electroanalysis 16, 1122 (2004). http://dx.doi.org/10.1002/elan.200403000
M. Senel and M. F. Abasiyanik, Electroanalysis 22, 1765 (2010). http://dx.doi.org/10.1002/elan.200900644
S. Mahiuddin, A. Renoncourt, P. Bauduin, D. Touraud and W. Kunz, Langmuir 21, 5259 (2005). http://dx.doi.org/10.1021/la047161r
M. Şenel, A. Coşkun, M. F. Abasiyanik and A. Bozkurt, Chemical Papers, 64, 1 (2010). http://dx.doi.org/10.2478/s11696-009-0103-x
P. B. Beata, M. Jan and L. Andrzej, Electrochim. Acta. 5, 12173 (2006). http://dx.doi.org/10.1016/j.electacta.2005.03.084
K. Uzun, E. Çevik, M. Şenel, H. Sözeri, A. Baykal, M. F. Abasiyanik and M. S. Toprak, J. Nanopart. 10, 3057 (2010). http://dx.doi.org/10.1007/s11051-010-9902-9
A. G. Hens, J. M. F. Romero and M. P. A. Caballos, Trends Anal. Chem. 27, 394 (2008). http://dx.doi.org/10.1016/j.trac.2008.03.006
G. Zhao, J. J. Xu and H. Y. Chen, Electrochem. Commun. 8, 148 (2006). http://dx.doi.org/10.1016/j.elecom.2005.11.001
T. Zhang, B. Z. Tian, J. L. Kong, P. Y. Yang and B. H. Liu, Anal. Chim. Acta 489, 199 (2003). http://dx.doi.org/10.1016/S0003-2670(03)00758-X
A. Vijayalakshmi, Y. Tarunashree, B. Baruwati, S. V. Manorama, B. L. Narayana, R. E. C. Johnson and N. M. Rao, Biosens. Bioelectron. 23, 1708 (2008). http://dx.doi.org/10.1016/j.bios.2008.02.003
A. Kaushik, R. Khan, P. R. Solanki, P. Pandey, J. Alam, S. Ahmad and B. D. Malhotra, Biosens. Bioelectron. 24, 676 (2008). http://dx.doi.org/10.1016/j.bios.2008.06.032
S. Qu, J. Wang, J. L. Kong, P. Y. Yang and G. Chen, Talanta 71, 1096 (2007). http://dx.doi.org/10.1016/j.talanta.2006.06.003
D. G. Zhang, Z. W. Tong, G. Y. Xu, S. Z. Li and J. J. Ma, Solid State Sci. 11, 113 (2009). http://dx.doi.org/10.1016/j.solidstatesciences.2008.05.001
A. E. G. Cass, G. Davis, G. D. Francis and H. A. O. Hill, Anal. Chem. 56, 667 (1984). http://dx.doi.org/10.1021/ac00268a018
Y. Kinemuchi, K. Ishizaka, H. Suematsu, W. H. Jiang and K. Yatsui, Thin Solid Films 407, 109 (2002). http://dx.doi.org/10.1016/S0040-6090(02)00021-4
A. Baykal, N. Kasapoglu, Yuksel Koseoglu, M. S. Toprak and H. Bayrakdar, J. Alloys Comp. 464, 514 (2008). http://dx.doi.org/10.1016/j.jallcom.2007.10.041
L. Luo, Q. Li, Y. Xu, Y. Ding, X. Wang, D. Deng and Y. Xu, Sensors and Actuators B 145, 293 (2010). http://dx.doi.org/10.1016/j.snb.2009.12.018
J. D. Liaso, S. P. Lin and Y. T. Wu, Biomacro-molecules 6, 392 (2005). http://dx.doi.org/10.1021/bm0494951
R. Khan, A. Kaushink, P. R. Solanki, A. A. Ansari, M. K. Pandey and B. D. Malhotra, Anal. Chim. Acta. 616, 207 (2008). http://dx.doi.org/10.1016/j.aca.2008.04.010
O. Carp, L. Patron and A. Reler, Mater. Chem. Phys. 101, 142 (2007). http://dx.doi.org/10.1016/j.matchemphys.2006.03.002
N. Kasapoglu, A. Baykal, Y. Koseoglu and M. S. Toprak, Scripta Mater. 57, 441 (2007). http://dx.doi.org/10.1016/j.scriptamat.2007.04.042
A. Baykal, N. Kasapoğlu, Y. Köseoğlu, A. C. Başaran, H. Kavas and M. S. Toprak, Cent. Eur. J. Chem. 6, 125 (2008). http://dx.doi.org/10.2478/s11532-007-0070-4
A. M. El Sayed, Ceram. Int. 28, 363 (2002). http://dx.doi.org/10.1016/S0272-8842(01)00103-1
A. Baykal, N. Kasapoglu, Y. Koseoglu, M. S. Toprak and H. Bayrakdar, J. Alloys Comp. 464, 514 (2008). http://dx.doi.org/10.1016/j.jallcom.2007.10.041
T. Wejrzanowski, R. Pielaszek, A. Opalinska, H. Matysiak, W. jkowski and K. J. Kurzydłowski, Appl. Surf. Sci. 253, 204 (2006). http://dx.doi.org/10.1016/j.apsusc.2006.05.089
R. Pielaszek, “Analytical expression for diffraction line profile for polydispersive powders”, in: Proceedings of the XIX Conference, Appl. Crystallography, World Scientific 2004, ISBN 981-238-761-8.
H. L. Zhang, X. Z. Zou, G. S. Lai, D. Y. Han and F. Wang, Electroanalysis 19, 1869 (2007). http://dx.doi.org/10.1002/elan.200703942
Z. Tong, R. Yuan, Y. Chai, Y. Xie and S. Chen, J. Biotechnol. 128, 567 (2007). http://dx.doi.org/10.1016/j.jbiotec.2006.12.008
R. A. Kamin and G. S. Willson, Anal. Chem. 52, 1198 (1980). http://dx.doi.org/10.1021/ac50058a010
M. Senel, E. Cevik and M. F. Abasiyanik, Sensors and Actuators B 145, 444 (2010). http://dx.doi.org/10.1016/j.snb.2009.12.055
Z. Tong, R. Yuan, Y. Chai, Y. Xie and S. Chen, J. Biotech. 128, 567 (2007). http://dx.doi.org/10.1016/j.jbiotec.2006.12.008
Hai-Li Zhang, G. Lai, D. Han and A. Yu, Anal. Bioanal. Chem. 390, 971 (2008).
A. K. M. Kafi, G. Wu and A. Chen, Biosens. Bioelectron. 24, 566 (2008). http://dx.doi.org/10.1016/j.bios.2008.06.004
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Yalçıner, F., Çevik, E., Şenel, M. et al. Development of an Amperometric Hydrogen Peroxide Biosensor based on the Immobilization of Horseradish Peroxidase onto Nickel Ferrite Nanoparticle-Chitosan Composite. Nano-Micro Lett. 3, 91–98 (2011). https://doi.org/10.1007/BF03353657
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03353657