Abstract
A simple and practical sensor of hydrogen peroxide (H2O2) was designed successfully. The mixture of horseradish peroxidase (HRP) and chitosan (Chit) are effectively immobilized on the surface of poly-L-leucine/polydopamine modified glassy carbon electrode (PL-LEU/PDA/GCE). Under the optimum conditions, the biosensor based on HRP exhibits a fast amperometric response (within 3 s) to H2O2. The linear response range of the sensor is 0.5–952.0 μmol L–1, with the detection limit of 0.1 μmol L–1 (S/N = 3) and the sensitivity of 0.23 A L moL–1 cm–2. The apparent Michaelis–Menten constant (k M app) of the biosensor is evaluated to be 0.12 mmol L–1, which suggests that the HRP-Chit/PL-LEU/PDA/GCE shows a higher affinity for H2O2. The sensor exhibits good sensitivity, selectivity, stability and reproducibility. The proposed method has been successfully applied to the determination of H2O2 in practical samples.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Luong, J.H.T., Nguyen, A.L., and Guilbault, G.G., Advances in Biochemical Engineering-Biotechnology, Berlin: Springer-Verlag, 1993, p. 85.
Lu, X.B., Zhou, J.H., Lu, W., Liu, Q., and Li, J.H., Biosens. Bioelectron., 2008, vol. 23, p. 1236.
Hurdis, E.C. and Romeyn, H., Jr., Anal. Chem., 1954, vol. 26, p. 320.
Matsubara, C., Kawamoto, N., and Takamura, K., Analyst, 1992, vol. 117 p. 1781.
Chen, S.H., Yuan, R., Chai, Y.Q., Zhang, L.Y., Wang, N., and Li, X.L., Biosens. Bioelectron., 2007, vol. 22, p. 1268.
Shi, C.G., Xu, J.J., and Chen, H.Y., J. Electroanal. Chem., 2007, vol. 610, p. 186.
Chang, M.C.Y., Pralle, A., Isacoff, E.Y., and Chang, C.J., J. Am. Chem. Soc., 2004, vol. 126, p. 15392.
Li, J., Dasgupta, P.K., and Tarver, G.A., Anal. Chem., 2003, vol. 75, p. 1203.
Zhang, L., Zhang, Q., Lu, X.B., and Li, J.H., Biosens. Bioelectron., 2007, vol. 23, p. 102.
Hamid, M. and Rehman, K., Food Chem., 2009, vol. 115, p. 1177.
Zhang, W.J. and Li, G.X., Anal. Sci., 2004, vol. 20, p. 603.
Ma, W. and Sun, D.M., Russ. J. Electrochem., 2007, vol. 43, p. 1382.
Liu, Y.G., Feng, X.M., Shen, J.M., Zhu, J.J., and Hou, W.H., J. Phys. Chem. B, 2008, vol. 2, p. 9237.
Wang, J., Anal. Chim. Acta, 1999, vol. 399, p. 21.
Jin, W. and Brennan, J.D., Anal. Chim. Acta, 2002, vol. 461, p. 1.
Shi, A.W., Qu, F.L., Yang, M.H., Shen, G.L., and Yu, R.Q., Sens. Actuat. B, 2008, vol. 129, p. 779.
Shen, J., Wang, W., Chen, Q., Wang, M.S., Xu, S.Y., Zhou, Y.L., and Zhang, X.X., Nanotechnology, 2009, vol. 20, p. 245307.
Wang, B., Zhang, J.J., Pan, Z.Y., Tao, X.Q., and Wang, H.S., Biosens. Bioelectron., 2009, vol. 24, p. 1141.
Roncali, J., Chem. Rev., 1992, vol. 92, p. 711.
Skotheim, T.A., Elsenbaumer, R.L., and Reynolds, J.R., 2nd ed., New York, Basel, Hong Kong: Marcel Delcker, 1998.
Groenendaal, L., Zotti, G., Auber, P.H., Waybright, S.M., and Reynold, J.R., Adv. Mater., 2003, vol. 15, p. 855.
Ma, W., Sun, D.M., Zhang, Z.X., and Ren, H.Z., Chin. J. Analysis Lab., 2005, vol. 24, p. 29.
Li, X. and Chen, M.F., Chin. J. Health Lab. Technol., 2009, vol. 19, p. 1770.
Zheng, X.Y., Guo, Y.H., Zheng, J.S., Zhou, X.H., Li, Q.L., and Lin, R.Y., Sens. Actuat. B, 2015, vol. 213, p. 188.
Salgado, R., Del Rio, R., and Del Valle, M.A., J. Electroanal. Chem., 2013, vol. 704, p. 130.
Palanisamy, S., Electrochim. Acta, 2014, vol. 138, p. 302.
Zhou, W.H., Lu, C.H., Guo, X.C., Chen, F.R., Yang, H.H., and Wang, X.R., J. Mater. Chem., 2010, vol. 20, p. 880.
Fu, Y.C., Li, P.H., Bu, L.J., Wang, T., Xie, Q.J., Xu, X.H., Lei, L.H., Zou, C., Chen, J.H., and Yao, S.Z., J. Phys. Chem. C, 2010, vol. 114, p. 1472.
Liu, K., Wei, W.Z., Zeng, J.X., Liu, X.Y., and Gao, Y.P., Anal. Bioanal. Chem., 2006, vol. 385, p. 724.
Lee, H., Dellatore, S.M., Miller, W.M., and Messersmith, P.B., Science, New York, N.Y., 2007, vol. 318, p. 426.
Kim, B., Son, S., Lee, K., Yang, H., and Kwak, J., Electroanal., 2012, vol. 24, p. 993.
Luczak, T., Electroanal., 2008, vol. 20, p. 1317.
Luczak, T., Electrochim. Acta, 2008, vol. 53, p. 5725.
Wang, F., Han, R., Liu, G.T., Chen, H., Ren, T., and Fang, H., J. Electroanal. Chem., 2013, vol. 706, p. 102.
Wang, A.J., Liao, Q.C., Feng, J.J., Yan, Z.Z., and Chen, J.R., Electrochim. Acta, 2012, vol. 61, p. 31.
Gu, Z.G., Yang, S.P., Li, Z.J., Sun, X.L., Wang, G.L., Fang, Y.J., and Liu, J.K., Anal. Chim. Acta, 2011, vol. 701, p. 75.
Hawley, M.D., Tatawawadi, S.V., Piekarski, S., and Adams, R.N., J. Am. Chem. Soc., 1967, vol. 89, p. 447.
Cui, L., Xu, M.Q., Zhu, J.Y., and Ai, S.Y., Synthetic Met., 2011, vol. 161, p. 1686.
Delvaux, M., Walcarius, A., and Demoustier-Champagne, S., Anal. Chim. Acta, 2004, vol. 525, p. 221.
Oungpipat, W., Alexander, P.W., and Southwell-Keely, P., Anal. Chim. Acta, 1995, vol. 309, p. 35.
Liu, M., Liu, R., and Chen, W., Biosens. Bioelectron., 2013, vol. 45, p. 206.
Niu, X., Zhao, H., Chen, C., and Lan, M., Electrochim. Acta, 2012, vol. 65, p. 97.
Kamin, R.A. and Wilson, G.S., Anal. Chem., 1980, vol. 52, p. 1198.
Chen, X., Li, C.C., Liu, Y.L., Du, Z.F., Xu, S.J., Li, L.M., Zhang, M., and Wang, T.H., Talanta, 2008, vol. 77, p. 37.
Chen, H.J. and Dong, S.J., Biosens. Bioelectron., 2007, vol. 22, p. 1811.
Xiao, Y., Ju, H.X., and Chen, H.Y., Anal. Chim. Acta, 1999, vol. 391, p. 299.
Lei, C.X., Hu, S.Q., Shen, G.L., and Yu, R.Q., Talanta, 2003, vol. 59, p. 981.
Ndangili, P.M., Waryo, T.T., Muchindu, M., Baker, P.G.L., Ngila, C.J., and Iwuoha, E.I., Electrochim. Acta, 2010, vol. 55, p. 4267.
Chut, S.L., Li, J., and Tan, S.N., Analyst, 1997, vol. 122, p. 1431.
Author information
Authors and Affiliations
Corresponding author
Additional information
Published in Russian in Elektrokhimiya, 2017, Vol. 53, No. 5, pp. 505–514.
The article is published in the original.
Rights and permissions
About this article
Cite this article
Zheng, X., Guo, Y., Zheng, J. et al. A hydrogen peroxide biosensor based on horseradish peroxidase/poly(L-leucine)/polydopamine modified glassy carbon electrode. Russ J Electrochem 53, 443–451 (2017). https://doi.org/10.1134/S1023193517050172
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1023193517050172