Abstract
A subgenomic library constructed from small Pstl restriction fragments (0.4 to 2.0 kb) yielded 83.18% low-copy clones. Using 17 random genomic and 5 heterologous probes in 65 probe-enzyme combinations, Restriction Fragment Length Polymorphism (RFLP) for nuclear DNA was studied in five desi and five kabuli type chickpea cultivars. Only two clones revealed polymorphism in the cultivars tested. No polymorphism in chickpea varieties was detected with four Random Amplified Polymorphic DNA (RAPD) markers studied. However, some degree of polymorphism between C. arietinum and its wild relative C. reticulatum was detected. The RFLP analysis of chloroplast and mitochondrial genomes showed no polymorphism.
Similar content being viewed by others
References
Muehlbauer, F J & Singh K B, In The Chickpea (MC Saxena, KB Singh, Editors), CAB International, Wallingford, Oxon (1987) pp 99–125.
Gaur PM & Slinkard AE, Theor Appl Genet, 80 (1990) 648.
Gill KS, Lubbers EL, Gill BS, Raupp WJ & Cox TS, Genome, 34 (1991) 362.
Havey MJ & Muehlbauer FJ, Tneor Appl Genet, 77 (1989) 395.
Helentijaris T, Slocum M, Wright S, Schaefer A & Nienhuis J, Theor Appl Genet, 72 (1986) 761.
McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR & Tanksley SD, Theor Appl Genet, 76 (1988) 815.
Song KM, Suzuki JV, Slocum MK, Williams PH & Osborn TC, Theor Appl Genet, 82 (1991) 292
Young ND, Zamir D, Ganal MW & Tanksley SD, Genetics, 120 (1988) 579.
Nienhuis J, Helentjaris T, Slocum M, Ruggero B & Schaefer A, Crop Sci, 27 (1987) 797.
Davis LG, Dibner MD & Battey JF, Basic methods in molecular biology, Elsevier Science Publishing Co. Inc., New York (1986).
Anderson MLM & Young BD, In Nudeic acid hybridisation -A practical approach (BD Hames, SJ Higgins, editors), IRL Press, Oxford (1985) pp 73–111.
Doyle JJ & Doyle JL, Focus, 12 (1990) 13.
Bernatzky R & Tanksley SD, Genetics, 112 (1986) 887.
Williams JG, Kubelik AE, Levak KJ, Rafalski JA & Tingey SC, Nucleic Acids Res, 18 (1990) 6531.
Miller JC & Tanksley SD, Theor Appl Genet, 80 (1990) 437.
Helentjaris T, King G, Slocum M, Sleden & Wegman S, Plant Mol Biol, 5 (1985) 109.
Kochert G, Halward T, Branch WD & Simpson CE, Theor Appl Genet, 81 (1991) 565.
Chase CD, Ortega VM & Vallejos CE, Theor Appl Genet, 81 (1991) 806.
Keim P, Shoemaker RC & Palmer RG, Theor Appl Genet, 77 (1988) 786.
Salimath PM, Bahl PN & Mehra RB, Plant Breed, 92 (1984) 52.
Moreno MT & Cubero JI, Euphytica, 27 (1978) 465.
Khairallah MM, Adams MW & Sears BB, Theor Appl Genet, 80 (1990) 753.
Ladizinsky G & Adler A, Theor Appl Genet, 48 (1976) 197.
Goodfellow PN, Nature, 359 (1992) 777.
Weissenbach J, Gyapay G, Dib C, Vignal A, Morissette J, Millasseau P, Vaysselx G & Lathrop M, Nature, 359 (1992) 794.
Weissing K, Kaemmer D, Weigand F, Epplen JT & Kahl G, Genome, 35 (1992) 436.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Udupa, S.M., Sharma, A., Sharma, R.P. et al. Narrow Genetic Variability in Cicer arietinum L. as Revealed by RFLP Analysis. J. Plant Biochem. Biotechnol. 2, 83–86 (1993). https://doi.org/10.1007/BF03262930
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03262930