Abstract
There are many advantages to carrying out electron paramagnetic resonance experiments at very high frequencies, either as part of a multifrequency strategy for solving problems or for special characteristics of high frequencies. These special characteristics include the potential for high point sensitivity, enhanced resolution, separation of similar species, altered sensitivity to motion, suppression of motional effects, and many others. This paper describes a three-millimeter-wavelength (W-band, 94 GHz) EPR spectrometer built for a multi-user facility and illustrates with some examples, most of them being disordered systems. One significant example is the oxidized primary reaction center, P +865 , isolated from the red photosynthetic bacteriaRhodobacter Sphaeroides R-26. The W-band technique applied to both centers isolated from bacteria grown from either deuterated or ordinary growth media allows extraction of the fullg anisotropy in these centers and sets the stage for multifrequency EPR spectroscopy to yield a full analysis of the various contributions to linewidths in these systems.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Grinberg O.Ya., Dubinskii A.A., Lebedev Ya.S.: Russian Chemical Reviews52, 850 (1983)
Belford R.L., Clarkson R.B. in: Magnetic Resonance of Carbonaceous Solids (Botto R.E., Sanada Y., eds.). ACS Advances in Chemistry Series229, 107–138. Washington DC: ACS 1993.
Belford R.L., Clarkson R.B., Cornelius J.B., Rothenberger K.S., Nilges M.J., Timken M.D. in: Electron Magnetic Resonance of the Solid State, (Weil J.A., ed.). CSC Symposium Series Vol. 1, 21. Ottawa: Canadian Society for Chemistry 1987.
Barra A.L., Brunel L.C., Robert J.B.: Chem. Phys. Lett.165, 107 (1990)
Haindl E., Möbius K., Oloff H.: Z. Naturforsch.40a, 169 (1985)
Box H.C., Freund H.G., Lilga K.T., Budzinski E.E.: J. Phys. Chem.74, 40 (1970)
Budil D.E., Earle K.A., Lynch W.B., Freed J.H. in: Advanced EPR in Biology and Biochemistry (Hoff A.J., ed.). Amsterdam: Elsevier 1989.
Lynch W.B., Earle K.A., Freed J.H.: Rev. Sci. Instrum.59, 1345 (1988)
Wagner R.J., White A.M.: Solid State Commun.32, 399 (1979)
Wang W., Clarkson R.B., Auteri F.P., Kovacs P., Williamson M.V., Belford R.L.: Am. Chem. Soc. Preprints, Div. Fuel Chem.37, 1141–1150 (1992)
Weber R.T., Disselhorst J.A.J.M., Prevo L.J., Schmidt J., Wenckebach W.Th.: J. Magn. Reson.81, 129 (1989)
Clarkson R.B., Wang W., Brown D.R., Crookham H.C., Belford R.L.: Fuel69, 1405 (1990)
Clarkson R.B., Wang W., Brown D.R., Crookham H.C., Belford R.L. in: Magnetic Resonance of Carbonaceous Solids (Botto R.E., Sanada Y., eds.). ACS Advances in Chemistry Series229, 507–528. Washington DC: ACS 1993.
Wang W.: Very High Frequency EPR: Instrument and Applications, Ph.D. Thesis, University of Illinois, Urbana-Champaign, IL, USA, April 1993 (Copies available through Dissertation abstracts, Ann Arbor, MI, USA)
Nilges M.J., Smirnov A.I., Belford R.L., Wei W., Clarkson R.B.: W-band (94 GHz) EPR System with Fast Sweep and Cryostat. 16th Intl. EPR Symposium at 35th Rocky Mountain Conference on Analytical Chemistry, Denver, CO, USA. July 27, 1993.
Poole Jr. C. P.: Electron Spin Resonance. A Comprehensive Treatise on Experimental Techniques. 2nd edition. New York: Wiley-Interscience 1983.
Wertz J. E., Bolton J. R.: Electron Spin Resonance. Elementary Theory and Practical Applications. New York: McGrow-Hill 1972.
Kuroda S., Motokawa M., Date M.: J. Phys. Soc. Japan44, 1797 (1978)
White L.K.: Ph. D. Thesis, University of Illinois, Urbana-Champaign, IL (1975)
Norris J.R., Uphaus R.A., Crespi H.L., Katz J.J.: Proc. Natl. Acad. Sci.68, 625 (1971)
Burghaus O., Plato M., Bumann D., Neumann B., Lubitz W., Möbius K.: Chem. Phys. Lett.185, 381 (1991)
Allen J.P., Feher G.: Proc. Natl. Acad. Sci. Us.81, 4795 (1984)
McElroy J.D., Feher G., Mauzarall D.C.: Biochim. Biophys. Acta267, 363–374 (1972)
Gerfen G., Griffin R.G.: MIT, private communication.
Prisner T.F., McDermott A.E., Un S., Norris J.R., Thurnauer M.C., Griffin R.G.: Proc. Nat. Acad. Sci. USA, accepted for publication.
Author information
Authors and Affiliations
Additional information
Adapted in substantial part from the PhD Dissertation of Wei Wang, University of Illinois, Urbana, April 1993.
Rights and permissions
About this article
Cite this article
Wang, W., Belford, R.L., Clarkson, R.B. et al. Very high frequency EPR — 94 GHz instrument and applications to primary reaction centers from photosynthetic red bacteria and to other disordered systems. Appl. Magn. Reson. 6, 195–215 (1994). https://doi.org/10.1007/BF03162490
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF03162490