Abstract
3T3-L1 cells can be induced to differentiate by exposing cell culture to a medium containing 3-isobutyl-l-methyl-xanthine together with insulin and dexamethasone. We have previously observed that oxalomalate which inhibits aconitase thus blocking the citric cycle, also favours differentiation to adipocytes leading to fatty acids accumulation and increased synthesis of specific proteins. We compared the effects of 3-isobutyl-l-methyl-xanthme-insulin-dexamethasone and on the steady-state levels of mRNA of c/EBP, H-ferritin and aconitase/IRE-BP. does not affect the steady-state mRNA amount of c/EBP, H-ferritin and aconitase/IRE-BP, but the 3-isobutyl-l-methyl-xanthine-insulin-dexamethasone mixture is able to induce a five-fold increase of mRNA levels for aconitase/IRE-BP. This is the first finding correlating iron metabolism to adipocyte differentiation. Further studies will clarify a possible role for the aconitase inhibitor.
Riassunto
Le cellule 3T3-L1 possono essere indotte a differenziarsi dall’aggiunta al mezzo di coltura di 3-isobutil-l-metil-xantina-insulina-desametasone. Noi abbiamo precedentemente osservato che anche l’ossalomalato noto inibitorc della aconitasi, put) indurre 1a differenziazione di 3T3-L1 con accumulo di acidi grassi e aumento della sintesi di proteine specifiche. Nel presente lavoro sono stati paragonati i livelli di mRNA per c/EBP, H-ierritina e aconitasi/IRE-BP in 3T3-L1 differenziate in seguito a trattamento con mezzi contenenti 3-isobutil-1-metil-xantina-insulina-desametasone owero ON1A. L’OMA non altera i livelli degli mRNA analizzati, ma l’aggiunta di 3-isobutil-l-metil-xantina-insulina-desametasone al mezzo di coltura fa aumentare di circa cinque volte il livello di mRNA per aconitasi/IRE-BP. Questi sono i primi dati che mostrano una correlazione tra il metabolismo del ferro e la differenziazione degli adipociti. Ricerche in corso chiariranno se l’OMA svolga un ruolo in tale correlazione.
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Nella sedura del 13 dicembre 1996.
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Festa, M., Colonna, A., Ruffo, A. et al. Modulation of gene expression in differentiating 3T3-L1 preadipocytes. Rend. Fis. Acc. Lincei 8, 111–116 (1997). https://doi.org/10.1007/BF03002325
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DOI: https://doi.org/10.1007/BF03002325