Abstract
Sorghum was inoculated withPseudomonas bacteria, including strains harboring an As-resistance plasmid, pBS3031, to enhance As-extraction by the plants.Pseudomonas strains (P.fluorescens 38a, P.putida 53a, and P.aureofaciens BS1393) were chosen because they are antagonistic to a wide range of phyto-pathogenic fungi and bacteria, and they can stimulate plant growth. The resistance of natural rhizospheric pseudomonads to sodium arsenite was assessed. Genetically modifiedPseudomonas strains resistant to As(III)/As(V) were obtained via conjugation or transformation. The effects of the strains on the growth of sorghum on sodium-arsenite-containing soils were assessed. The conclusions from this study are: (1) It is possible to increase the survivability of sorghum growing in sodium-arsenite-containing soil by using rhizosphere pseudomonads. (2) The presence of pBS3031 offers the strains a certain selective advantage in arsenite-contaminated soil. (3) The presence of pBS3031 impairs plant growth, due to the As-resistance mechanism determined by this plasmid: the transformation of the less toxic arsenate into the more toxic, plant-root-available arsenite by arsenate reductase and the active removal of arsenite from bacterial cells. (4) Such a mechanism makes it possible to develop a bacteria-assisted phytoremediation technology for the cleanup of As-contaminated soils and is the only possible way of removing the soil-sorbed arsenates from the environment.
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Abbreviations
- CFU:
-
Colony-Forming Unit
- LB:
-
Luria-Bertoni
- MIC:
-
Minimum Inhibitory Concentration
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Sizova, O.I., Kochetkov, V.V., Validov, S.Z. et al. Arsenic-contaminated soils genetically modifiedPseudomonas spp. and their arsemc-phytoremediation potential. J Soils & Sediments 2, 19–23 (2002). https://doi.org/10.1007/BF02991246
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DOI: https://doi.org/10.1007/BF02991246