Abstract
Mature inflorescences of 76 samples of ten genera of native grasses were collected in the Ankara province of Turkey. Microscopic examination of individual seeds revealed the presence of endophytic fungi in four seed samples, The endophytes were isolated in pure culture and identified. Seeds were also ground and subjected to ELISA and HPLC analyses to confirm the levels of ergot alkaloids typically produced by endophytic fungi of grasses.
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References
Freeman, E.M. (1902) The seed fungus ofLolium temulentum L.Darnel Philos. Trans. 196:1–29.
Johnson, M.C., Pirone, T.P., Siegel, M.R. and Varney, DR. (1982) Detection ofEpichloe typhina in tall fescue by me;ins of enzyme linked immunosorbent assay.Phytopathology 72:647–650.
Latch, G.C.M. (1993) Physiological interaction of endophytic fungi and their hosts. Biotic stress tolerance imparted to grasses by endophytes.Agric. Ecosyst. & Environ. 44:143–156.
Musgrave, G., Latch, G.C.M. and Christensen, M.J. (1986) Purification and characterization of antigens of endophytic fungi isolated fromLolium perenne andFestuca arundinacea in New Zealand.N.Z. J. Agric. Res. 29:121–128.
Neil, J.C. (1941) The endophytesof Lolium andFestuca.N.Z.J. Sci. Technol. Sect. A 23:185–193.
Rosenthaler, J. and Munzer, H. (1976) 9,10-dihydroergotamine: production of antibodies and radioim- munoassay.Experientia 32:234–236.
Shelby, R.A. and Kelley, V.C. (1991) Detection of ergot alkaloids in tall fescue by competitive immunoassay with a monoclonal antibody.Food & Agric. Immunol. 3:169–177.
Shelby, R.A., Olsovska, J., Havlicek, V. and Flieger, M. (1997) Analysis of ergot alkaloids in endophyte- infected tall fescue by liquid chromatography/ electrospray ionization mass spectrometry.J. Agric. Food Chem. 45:4674–4679.
Siegel, MR., Latch, G.C.M., Bush, L.P., Fannin, F.F., Rowan, D.D., Tapper, B.A., Bacon, C.W. and Johnson, M.C. (1990) Fungal endophyte infected grasses. Alkaloid accumulation and aphid response.J. Chem. Ecol. 16:3301.
Siegel, M.R., Latch, G.C.M. and Johnson, M.C. (1985)Acremonium fungal endophytes in tall fescue and perennial ryegrass: Significance and control.Plant Dis. 69:179–183.
White, J.F. and Cole, G.T. (1985) Endophyte host associations in forage grasses. I. Distribution of fungal endophytes in some species ofLolium andFestuca.Mycologia 77:323.
Yates, S.G. and Powell, R.G. (1988) Analysis of ergopeptine alkaloids in endophyte-infected tall fescue.J. Agric. Food Chem. 36:337–340.
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Tunali, B., Shelby, R.A., Morgan-Jones, G. et al. Endophytic fungi and ergot alkaloids in native Turkish grasses. Phytoparasitica 28, 375–377 (2000). https://doi.org/10.1007/BF02981832
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DOI: https://doi.org/10.1007/BF02981832