Abstract
In order to isolate substances that inhibit the hemolytic activity of human serum against erythrocytes, we have evaluated whole plants of theOrostachys japonicus species with regard to its anti-complement activity, and have identified its active principles following activity-guided isolation. A methanol extract of theO. japonicus, as well as itsn-hexane soluble fraction, exhibited significant anti-complement activity on the complement system, which was expressed as total hemolytic activity. A bioassay-guided chromatographic separation of the constituents resulted in the isolation of three known compounds1–3 from the activen-hexane fraction. The structure of these compounds were analyzed, and they were identified as hydroxyhopanone (1), β-sitosteryl-3-O-β-d-glucopyranosyl-6′-O-palmitate (2), and β-sitosteryl-3-O-β-d-glucopyranoside (3), respectively. Of these compounds, compound2 exhibited potent anti-complement activity (IC50=1.0±0.1 μM) on the classical pathway of the complement, as compared to tiliroside (IC50=76.5±1.1 μM), which was used as a positive control. However, compounds1 and3 exhibited no activity in this system.
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Yoon, N.Y., Min, B.S., Lee, H.K. et al. A potent anti-complementary acylated sterol glucoside fromOrostachys japonicus . Arch Pharm Res 28, 892–896 (2005). https://doi.org/10.1007/BF02973873
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DOI: https://doi.org/10.1007/BF02973873