Abstract
The genome sequencing project has generated and will continue to generate enormous amounts of sequence data. Since the first complete genome sequence of bacteriumHacmophilus influenzac was published in 1995, the complete genome sequences of 2 eukaryotic and about 22 prokaryotic organisms have been determined. Given this ever-increasing amounts of sequence information, new strategies are necessary to efficiently pursue the next phase of the genome project—the elucidation of gene expression patterns and gene product function on a whole genome scale. In order to assign functional information to the genome sequence, DNA chip technology was developed to efficiently identify the differential expression pattern of independent biological samples. DNA chip provides a new tool for genome expression analysis that may revolutionize many aspects of human life including new drug discovery and human disease diagnostics.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Flischmann, R. D.et al. (1995) Whole-genome random sequencing and assembly ofHaemorphilus influenzac Rd.Science 269: 496–512.
http://www.ncbi.nlm.nih.gov/Entrez/Genome/org.html.
Alwine, J. C., D. J. Kemp, and G. R. Stark (1977) Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.Proc. Natl. Acad. Sci. USA 74: 5350–5354.
Zinn, K., D. DiMaio, and T. Maniatis (1983) Identification of two distinct regulatory regions adjacent to the human β-interferon gene.Cell 34: 865–879.
St. John, T. P., and R. W. Davis (1979) Isolation of galactose-inducible DNA sequences fromSaccharomyces cercvisiae by differential plaque filter hybridization.Cell 16: 443–452.
Schena, M., D. Shalon, R. W. Davis, and P. O. Brown (1995) Quantitative monitoring of gene expression parterns with a complementary DNA microarray.Science 270: 467–470.
Hwang, S. Y. (1999) DNA chip technologies. Array of hope.Korean Soc. Med. Biochem. Mol. Biol. News 6: 19–23.
http://www.genechip.co.kr.
Duggan, D. J., M. Bittner, Y. Chen, P. Meltzer, and J. M. Trent (1999) Expressionprofiling using cDNA, microarrays.Nat. Genet. Suppl. 21: 10–14.
http://www.ncbi.nlm.nih.gov/CGAP/
Theriault, T. P., S. C. Winder, and R. C. Gamble (1999) Application of ink-jet printing technology to the manufacture of molecular arrays. pp. 101–120. In:DNA Microarrays. Oxford, UK.
http://www.affymetrix.com/
Hwang, S. Y. (1997) Whole genome analysis using DNA chips.Korean Soc. Med. Biochem. Mol. Biol. News 4: 23–34.
Lipshutz, R. J., S. P. A. Fodor, T. R. Gingeras, and D. J. Lockhart (1999) High density synthetic oligonucleotide arrays.Nat. Genet. Supp. 21: 20–24.
Singh-Gasson, S., R. D. Green, Y. Yue, C. Nelson, F. Blattner, M. R. Sussman, and E. Cerrina (1999) Maskless fabrifabri-cation of light-directed oligonucleotide microarrays using a digital micromirror array.Nat. Biotechnol. 17: 974–973.
http://www.nanogen.com.
Holler, M. J., E. Tu, A. Holmsen, R. G. Sosnowski, and J. Olconnel (1999) Active microelectronic arrays for DNA hybridization analysis. pp. 167–185. In:DNA Microarrays, Oxford, UK.
Debouck, C., and P. N. Goodfellow (1999) DNA microarrays in drug discovery and development.Nat. Cenet. Supp. 21: 48–50.
Bassett Jr., D. E., M. B. Eisen, and M. S. Boguski (1999) Gene expression informatics—it's all in your mine.Nat. Genet. Supp. 21: 51–55.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hwang, S.Y., Lim, G. DNA Chip technologies. Biotechnol. Bioprocess Eng. 5, 159–163 (2000). https://doi.org/10.1007/BF02936587
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02936587